Human Granzyme B Quantikine ELISA Kit DGZB00 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman Granzyme B Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDGZB00
Quantity1 Kit
ApplicationsELISA
Detection Range31.30 - 2000 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (50 uL)
Sensitivity4.8 pg/mL
Detection TargetGranzyme B
Assay Time3.5 hours
NCBI Full Gene Namegranzyme B
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Gene AliasesC11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Dissecting age-specific genetic architecture of vitiligo through integrative Post-GWAS analysisFront ImmunolMay 8, 2026Qingqing Si, Shenglan Wang, Junru Chen, Yuanyu Feng, Ruike Zhao, Dongjie Sunand TEF were determined using commercially available ELISA kits (KE00402, proteintech for RNASET2; DGZB00, R&D Systems for GZMB; and HT0124, Kendall Scientific for TEF) according to the manufacturers’ instructions.

(2) Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: insights from in vitro assaysFrontiers in ImmunologyFebruary 6, 2026Miriam Kelm, Nourhan Nasr, Sonja Bendig, Dieter Kabelitz, Marta Lustig, Heiko Trautmann, Anna Laqua, Christian Peters, Daniela Wesch, Hans-Heinrich Oberg, et al.Enzyme-linked immunosorbent assay Cell culture supernatants were analyzed for Interferon-γ (IFNγ; DIF50C), Granzyme B (GrzB; DGZB00), Perforin (QK8011), Granulysin (Gnly; DY3138), Perforin (Prf; QK8011) and soluble Fas-Ligand (sFAS-L;

(3) HBx hijacks the miR‐19a‐3p/BAMBI/TGF‐β1 axis to impair the anti‐tumour activity of CD4 + T cells in diffuse large B‐cell lymphomaClinical and Translational MedicineJanuary 5, 2026Xuecong Guo, Jianguo Li, Xiaofei Bai, Yinghui Huang, Xu Xu, Jiabang Yang, Zhenghao Sun, Wangcheng Zhu, Xudong Guo, Jie Chen, et al.Granzyme B, and TGF‐β1 were measured using human‐specific ELISA kits (Absin, # abs510007; R&D Systems, # DGZB00; Jianglai Bio, # JL10706) and mouse‐specific ELISA kits for syngeneic models (Absin, # abs520007; Jianglai

(4) Dipeptidyl peptidase 4 restoration facilitates anti-tumor immunity in KRAS-LKB1-mutant lung cancerCancer Research CommunicationsDecember 1, 2025Toshiyuki Tenma, Ryohei Yoshida, Hiraku Yanada, Kiichi Nitanai, Toshihiro Nagato, Kyohei Oyama, Nobunari Sasaki, Mizuki Homme, Chie Mori, Takayuki Ohkuri, et al.Signatures Database ontology gene set was used for the analysis. ELISA Human granzyme B (R&D Systems, DGZB00) ELISAs were performed according to the manufacturer’s instructions. Conditioned media were collected

(5) Dipeptidyl Peptidase 4 Restoration Facilitates Antitumor Immunity in KRAS-LKB1–Mutant Lung CancerCancer Res CommunDecember 1, 2025Toshiyuki Tenma, Ryohei Yoshida, Hiraku Yanada, Kiichi Nitanai, Toshihiro Nagato, Kyohei Oyama, Nobunari Sasaki, Mizuki Homme, Chie Mori, Takayuki Ohkuri, et al.Signatures Database ontology gene set was used for the analysis. ELISA Human granzyme B (R&D Systems, DGZB00) ELISAs were performed according to the manufacturer’s instructions. Conditioned media were collected

(6) Reduced ALDH1A1 expression in multiple myeloma cells increases resistance to daratumumab via downregulation of retinoic acidCell Mol Life SciOctober 7, 2025Sujie Zheng, Jing He, Xiaolu Song, Ye Peng, Wufeng Yuan, Xiangmin Tongand INF-gamma measurement Commercial enzyme-linked immunoabsorbent assay (ELISA) kits (NBP3-11726, DGZB00, and DIF50C, Novus Biologicals) were used measuring above granule contents, following protocols reported

(7) Cell-targeted PD-1 agonists are potent NK-cell inhibitorsFrontiers in ImmunologyAugust 22, 2025Harriet C. Pope, Ana L. Chiodetti, Alice Newey, Guillaume Rieunier, David X. Overton, Eduardo Mateos-Diaz, Tara M. Mahon, Giovanna Bossi, Hussein Al-Mossawi, Charlotte Viantsupernatant of NK92-PD-1 and NHDF cultures were collected at the indicated time point and measured by ELISA (DGZB00 and DY275-05, Bio-Techne Ltd.), according to the manufacturer’s protocol. Flow cytometry Samples were

(8) Immune targeting of triple-negative breast cancer through a clinically actionable STING agonist-CAR T cell platformCell Reports MedicineJuly 15, 2025Yuqing Zhang, Zehua Li, Jessica Ritter, Elliott J. Brea, Navin R. Mahadevan, Deborah A. Dillon, Sung-Rye Park, Eric Minwei Liu, Michael Y. Tolstorukov, William E. McGourty, et al.,R&D Systems,Cat# DIFNB0 Human IFNγ ELISA kit,R&D Systems,Cat# DIF50C Human Granzyme B ELISA kit,R&D Systems,Cat# DGZB00 Human TNFα ELISA kit,R&D Systems,Cat# DTA00D CellTiter-Glo Luminescent cell viability assay kit,Promega,Cat#

(9) Thrombospondin-1 induces CD8+ T cell exhaustion and immune suppression within the tumor microenvironment of ovarian cancerJ Ovarian ResMay 10, 2025Haiyan Liang, Suwei ZhangCCL1 (#DY272), IL-4 (#D4050), TNF-α (#DTA00D), IL-1β (#DY201), IL-6 (#D6050B), PFN (#QK8011), GZMB (#DGZB00), IFN-γ (#QK285), and IL-2 (#QK202) (R&D Systems). Supernatants were harvested after 48 h of culture,

(10) Trabectedin Enhances the Antitumor Effects of IL-12 in Triple-Negative Breast CancerCancer immunology researchApril 2, 2025Schwarz E, Savardekar H, Zelinskas S, Mouse A, Lapurga G, Lyberger J, Rivaldi A, Ringwalt EM, Miller KE, Yu L, et al.(R&D Systems Cat. #DY285B-05), TNF-α (R&D Systems Cat. #DY210-05), and granzyme B (R&D Systems Cat. #DGZB00) by ELISAs according to the manufacturer’s instructions. Statistical analyses were performed in GraphPad

(11) RNA sensing induced by chromosome missegregation augments anti-tumor immunityMolecular CellFebruary 20, 2025Nobunari Sasaki, Mizuki Homme, Takahiko Murayama, Tatsuya Osaki, Toshiyuki Tenma, Tadaichi An, Yujiro Takegami, Tetsuo Tani, Patrick C. Gedeon, Yoshihisa Kobayashi, et al.(R&D systems, Cat.# DIF50C), human CXCL10 (R&D systems, Cat.# DIP100), human Granzyme B (R&D systems, Cat.# DGZB00), mouse IFN-β (R&D systems, Cat.# MIF00), mouse CXCL10 (R&D systems, Cat.# DY466), and 2’3’-cGAMP (Cayman

(12) A Novel Tetravalent Bispecific Immune Cell Engager Activates Natural Killer Cells to Kill Cancer Cells without Mediating FratricideAntibodies (Basel, Switzerland)September 10, 2024Yang G, Nikkhoi SK, Owji H, Li G, Massumi M, Cervelli J, Vandavasi VG, Hatefi A.(R&D Systems, Cat# DY210-05), IFN-γ (R&D Systems, Cat# DY285B-05), and granzyme B (R&D Systems, Cat# DGZB00) were measured using ELISA kits following the manufacturers’ protocols. 3. Results and Discussion 3.1.

(13) Evaluation of resazurin phenoxazine dye as a highly sensitive cell viability potency assay for natural killer cell‐derived extracellular vesicle‐based cancer biotherapeuticsJournal of Extracellular BiologyJuly 17, 2024Frederic St‐Denis‐Bissonnette, Shirley Qiu, Sarah E. Cummings, Melanie Kirkby, Yohannes Haile, Sarah Wassmer, Gauri Muradia, Jelica Mehic, Andrew Stalker, Amit Shrestha, et al.NK‐EVs’ corona: FasL/TNFSF6 (R&D Systems, cat#DFL00B), GNLY (Abcam, cat#ab256402), GzmB (R&D Systems, cat#DGZB00), PFN (Abcam, cat#ab46068) and IFN‐γ (R&D Systems, cat#DIF50C). Readings were made using the Microplate

(14) Define Critical Parameters of Trastuzumab-Mediated ADCC Assays via Assay Optimization Processes, Focusing on the Impact of Cryopreserved Effector Cells on Assay PerformanceCancersJune 27, 2024Peng H, Endo Y, Wu WJ.Human Perforin ELISA Kit (PRF1) (Abcam, Cat# ab46068, Waltham, MA, USA), Human Granzyme B Quantikine ELISA Kit (R&D Systems, Cat# DGZB00), Human IFN-gamma Quantikine ELISA Kit (R&D Systems, Cat# DIF50C), and Human TNF-alpha Quantikine ELISA Kit

(15) Chemotherapy-induced high expression of IL23A enhances efficacy of anti-PD-1 therapy in TNBC by co-activating the PI3K-AKT signaling pathway of CTLsScientific reportsJune 20, 2024Pan F, Liu J, Chen Y, Zhu B, Chen W, Yang Y, Zhu C, Zhao H, Liu X, Xu Y, et al.

(16) Structural and functional characterization of IgG- and non-IgG-based T-cell-engaging bispecific antibodiesFrontiers in ImmunologyMay 28, 2024Nishant Mohan, Safiat Ayinde, Hanjing Peng, Shraboni Dutta, Yi Shen, Vincent M. Falkowski, Thomas G. Biel, Tongzhong Ju, Wen Jin Wuarray Quantitative measurement of Granzyme B was performed using Human Granzyme B Quantikine ELISA Kit (cat# DGZB00, R and D Systems, Minneapolis, MN) and perforin was measured using Perforin (PRF1) Human ELISA Kit (cat#

(17) D2HGDH-mediated D2HG catabolism enhances the anti-tumor activities of CAR-T cells in an immunosuppressive microenvironmentMolecular TherapyMarch 1, 2022Quanjun Yang, Juan Hao, Mengyi Chi, Yaxian Wang, Jie Li, Jinlu Huang, Jianping Zhang, Mengqi Zhang, Jin Lu, Shumin Zhou, et al.the supernatant was collected and analyzed for secretion of the cytokines IFN-γ (DIF50C), granzyme B (DGZB00), and TNF-α (DTA00D) with cytokine-specific ELISA kits (R&D Systems, Minneapolis, MN, USA) according

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Granzyme B ELISA

Granzyme B ELISA

Granzyme B ELISA

Granzyme B ELISA

Human Granzyme B Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human Granzyme B Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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