Fig 1: Muc5achigh airway secretory cells communicate with CD4+ T cells and neutrophils in inhaled-allergen-experienced lungs(A) Representative histogram plots depicting surface expression patterns of MHC class II, costimulatory molecules CD40, CD80, and CD86, and coinhibitory molecules PD-L1 and PD-L2 on distinct epithelial cells from LH mice at baseline.(B) Heatmap depicting fold change in surface expression levels of specified APC-related molecules on distinct epithelial cells from LH mice 24 h post-OVA challenge normalized to their baseline counterparts. N.D., not detected above FMO control. Mann-Whitney test.(C and D) mRNA levels of Muc5ac (C) and CXCL5 (D) in fluorescence-activated cell-sorted epithelial cells isolated from LH mice 24 h post-OVA challenge. One-way ANOVA with Fisher’s LSD test. *p ≤ 0.05.(E) Representative immunofluorescent micrographs showing anatomical location of Scgb1a1+ secretory epithelial cells (green), CD4+ T cells (red), and Ly6G+ neutrophils (magenta) in LH lungs 8 h post-OVA challenge. DAPI (blue) was used as a counterstain to identify lung structures. Data represent n = 3 mice/time point, two experiments.(F–I) t-SNE projection of scRNA-seq data depicting expression patterns for designated markers of interest by different subsets of epithelial cells identified in airways of adult asthmatic and healthy humans as displayed on the interactive web portal: https://asthma.cellgeni.sanger.ac.uk/.14All data in (B)–(D) have n = 4 mice, 2 experiments, and mean ± SEM.