Description
Our Caspase-3,7 Assay Kit, Colorimetric provides a simple and convenient method for assaying the activity of caspases 3 and 7, which is based on the hydrolysis of the labeled substrate Ac-DEVD-pNA (acetyl-Asp-Glu-Val- Asp p-nitroanilide) by Caspase-3 and 7, releasing the pNA (p-nitroaniline) moiety from the substrate. The released pNA has the max absorbance at 405 nm (εmM = 10.5) and its concentration is calculated by measuring the absorbance values at 405 nm or from a standard calibration curve prepared using the pNA Dye Standard, using a microplate reader or spectrophotometer