Fig 1: Metabolic enhancer and exercise independently protect against diet-induced liver injury. (A) Immunofluorescent staining of fibronectin in the livers of mice fed NCD or HFD supplemented with either ME or exercise. (B) Quantification of picro-sirius red (PSR) and trichrome staining livers. (C) Analysis of serum ALT activity (n = 8–11). (D,E) Quantitative real-time PCR analysis of mRNA hepatic abundance of indicated genes (n = 5–6). Scale bars, 200 µm. All data are shown as mean ± SD. *, denotes p < 0.05 by one-way ANOVA with Tukey multiple comparison testing using Prism 6 (GraphPad).