Description
The Mouse Immunoglobulin Isotyping ELISA qualitatively detects Mouse Ig isotypes in mouse hybridoma cell culture supernatants.
Principle of the method
Biotinylated monoclonal anti-mouse isotype specific or light chain specific capture antibodies bind to the Streptavidin coated plate. Ig Isotype present in the positive control or sample binds to the specific capture antibodies and is simultaneously bound by the HRP-conjugated detector antibody. Following incubation unbound reagents and sample components are removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of soluble mouse Ig (H+L) present in the sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested. See manual for more information on validation