ATPase Assay Kit (Colorimetric) ab234055 from Abcam

Product Specs
ItemATPase Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab234055
Quantity100 Test
Sample TypeCell Lysate, Tissue Lysate
ApplicationsEnzyme activity assay
Detection Range1 - 5 nmol/well

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(1) Sea buckthorn seed oil triggers mitochondrial dysfunction and apoptosis in Aspergillus niger by activating the AGE-RAGE-like signaling pathwayFrontiers in MicrobiologyApril 21, 2026Yanhua Xin, Aiyu Qin, Jie Yang, Yixin Shen, Jing Li, Lanying Guo, Bin Liang, Jing Suab228560), and ATPase activity was evaluated using the ATPase Assay Kit (Colorimetric) (Abcam, Cat. # ab234055). 2.14 Real-time fluorescence quantitative PCR analysis Transcript levels of AGE-RAGE signaling pathway

(2) Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosisRedox BiologyJanuary 30, 2026Qiaoyun Bai, Ningpo Ding, Rixin Feng, Fengxiang Shang, Zongqi Wang, Liangchang Li, Zhiguang Wang, Yihua Piao, Guangyu Jin, Yilan Song, et al.extracting total cellular protein, removing endogenous phosphate interference, using ATPase Assay Kit (Cat# ab234055, Abcam) to react in buffer containing ATP substrate, and measuring released inorganic phosphate by colorimetry.

(3) Structure and mechanism of an actin-dependent bacterial phosphoryl AMPylaseNature Chemical BiologyJanuary 1, 2026Tao-Tao Chen, Qiuhua Lu, Si-Ru Zheng, Jiaqi Fu, Jing Chen, Lina Kang, Juhong Wu, Jiwei Luo, Jiangyang Tong, Siying Li, et al.phosphate and pyrophosphate was respectively quantified by a colorimetric ATPase activity assay kit (Abcam, ab234055) and a fluorimetric pyrophosphate assay kit (YEASEN, 50115ES70) according to the manufacturer’s manual. To

(4) PAD2 disturbs cardiomyocyte calcium homeostasis by citrullinating SERCA2a protein in hemorrhagic shock induced arrhythmiaJournal of Trauma and Acute Care SurgeryAugust 1, 2025Lebin Gan, Rui Li, Qiang Wang, Jing Zhou, Mengwei Zhang, Minghong Leng, Junhui Zhao, Fenghe Yang, Shi Jia, Wei Huang, et al.ultracentrifugation. SERCA2a activity in 10 μg/mL samples was measured via ATPase assay kit (Abcam ab234055), calculating ATP hydrolysis normalized to protein content. Protein expression analyzed by immunoblotting. Statistical

(5) Au(I)-based compounds inhibit nsp14/nsp10 and nsp13 (helicase) to exert anti-SARS-CoV-2 propertiesJBIC Journal of Biological Inorganic ChemistryJune 18, 2025Jingxin Chen, Xueying Wei, Chun-Lung Chan, Kaiming Tang, Shuofeng Yuan, Hongyan Li, Hongzhe Sunactivities of gold-based compounds against SARS-CoV-2 helicase were assayed using a commercial assay kit (ab234055, Abcam). The experiments were performed following the manufacturer’s instruction. In brief, we pre-incubated

(6) FXYD2 marks and regulates maturity of β cells via ion channel-mediated signal transductionNat CommunJune 4, 2025Clarissa Tacto, Meghan Tahbaz, Andrew Salib, Shudi Wang, Fritz Cayabyab, Jinhyuk Choi, Kiyoka Kim, Yu Hamba, Harvey Perez, Paul D. Gershon, et al.instructions. Na+/K+ ATPase assay and Sodium assays Na+/K+ ATPase assay was performed using ATPase assay Kit (Abcam, ab234055). To determine the intracellular sodium concentration, Sodium Assay Kit (Abcam, ab211096) was used.

(7) PAD2 disturbs cardiomyocyte calcium homeostasis by citrullinating SERCA2a protein in hemorrhagic shock induced arrhythmiaJ Trauma Acute Care SurgMay 1, 2025Lebin Gan, Rui Li, Qiang Wang, Jing Zhou, Mengwei Zhang, Minghong Leng, Junhui Zhao, Fenghe Yang, Shi Jia, Wei Huang, et al.ultracentrifugation. SERCA2a activity in 10 μg/mL samples was measured via ATPase assay kit (Abcam ab234055), calculating ATP hydrolysis normalized to protein content. Protein expression analyzed by immunoblotting. Statistical

(8) Microglial progranulin differently regulates hypothalamic lysosomal function in lean and obese conditions via cleavage-dependent mechanismsJ NeuroinflammationMarch 7, 2025Chae Beom Park, Chan Hee Lee, Gil Myoung Kang, Se Hee Min, Min-Seon KimChemistry, #937), cathepsin D (Abcam, ab65302), cathepsin L (Immune Chemistry, #941), and v-ATPase (Abcam, ab234055) were assayed in the mediobasal hypothalamus (MBH) using commercial kits in accordance with the manufacturer’s

(9) Inhibition of Hsp90 K284 Acetylation Aalleviates Cardiac Injury After Ischemia-Reperfusion InjuryJournal of cardiovascular translational researchDecember 1, 2024Zhan D, Zhang N, Zhao L, Sun Z, Cang C.supernatant was subsequently harvested. Samples were reacted with Reaction Mix (ATPase Assay kit, Abcam, ab234055, England) at 25 °C for 30 min. Then, 30 µL ATPase Assay Developer was added to the reactions and incubated

(10) Urolithin A Protects Hepatocytes from Palmitic Acid-Induced ER Stress by Regulating Calcium Homeostasis in the MAMBiomoleculesNovember 26, 2024Gayoung Ryu, Minjeong Ko, Sooyeon Lee, Se In Park, Jin-Woong Choi, Ju Yeon Lee, Jin Young Kim, Ho Jeong Kwonmeasurement of ATPase activity. The ATPase activity was determined using an ATPase assay kit (Abcam, ab234055) according to the manufacturer’s instructions. 2.10. Lipid Droplet Staining Cells were seeded in 12-well

(11) The Pvc15 ATPase selectively associates effector proteins with the Photorhabdus virulence cassetteRoyal Society Open ScienceOctober 23, 2024Rhys Evans, Nicholas R. Waterfield530–570 nm, emission = 580–620 nm. 2.5. ATPase activity The colorimetric ATPase assay kit (Abcam, ab234055) was used. 15 ml of E. coli BL21(DE3) cultures were grown to OD600 = 1.0 and induced with 0.2% arabinose

(12) Myosin inhibitor reverses hypertrophic cardiomyopathy in genotypically diverse pediatric iPSC-cardiomyocytes to mirror variant correctionCell Reports MedicineMay 21, 2024Caroline Kinnear, Abdelrahman Said, Guoliang Meng, Yimu Zhao, Erika Y. Wang, Naimeh Rafatian, Neha Parmar, Wei Wei, Filio Billia, Craig A. Simmons, et al.Transfection Reagent,ThermoFisher,Cat# STEM00015 Myco Alert kit,Lonza,Cat# LT07-418 ATPase assay kit,Abcam,Cat# Ab234055 ,, Deposited data,Deposited data,Deposited data ,, Whole genome sequencing,European Genome-Phenome

(13) Anti-apoptotic MCL-1 promotes long-chain fatty acid oxidation through interaction with ACSL1Molecular cellApril 4, 2024Wright T, Turnis ME, Grace CR, Li X, Brakefield LA, Wang YD, Xu H, Kaminska E, Climer LK, Mukiza TO, et al.ab273315 Seahorse XF Cell Mito Stress Test Kit,Agilent,Cat# 103015-100 ATPase Assay Kit (Colorimetric),Abcam,Cat# ab234055 Malachite Green Phosphate Assay Kit,Millipore-Sigma,Cat# MAK307 Pierce BCA Protein Assay Kit,Thermo Fisher Scientific

(14) Targeting NEDD8 suppresses surgical stress-facilitated metastasis of colon cancer via restraining regulatory T cellsCell Death & DiseaseJanuary 5, 2024Yi Jiang, Shenjia Gao, Hao Sun, Xinyi Wu, Jiahui Gu, Han Wu, Yun Liao, Ronen Ben-Ami, Changhong Miao, Rong Shen, et al.assay kit (Cot # C2006, Beyotime). ATPase activity in Tregs was measured by ATPase Assay Kit (Cot # ab234055, Abcam). ROS content in Tregs was measured by DCFDA/H2DCFDA–Cellular ROS Assay Kit (Cot # ab113851,

(15) M2 macrophage-derived exosomes suppress tumor intrinsic immunogenicity to confer immunotherapy resistanceOncoImmunologyDecember 31, 2023Naisheng Zheng, Tingting Wang, Qin Luo, Yi Liu, Junyao Yang, Yunlan Zhou, Guohua Xie, Yanhui Ma, Xiangliang Yuan, Lisong Shenin Supplementary Table S8. ATPase assay ATPase Assay Colorimetric kits were used for experiments (ab234055, Abcam). Briefly, reaction samples were prepared for each 10 min time point between 0 and 60 min. BiP

(16) Positive Allosteric Modulators of SERCA Pump Restore Dendritic Spines and Rescue Long-Term Potentiation Defects in Alzheimer’s Disease Mouse ModelInternational Journal of Molecular SciencesSeptember 12, 2023Anastasiya Rakovskaya, Alexander Erofeev, Egor Vinokurov, Ekaterina Pchitskaya, Russell Dahl, Ilya BezprozvannyATPase assays in HEK 293 lysates were carried out using the ATPase Assay Kit (Colorimetric; Cat. No. ab234055; Abcam, Cambridge, UK) as per the manufacturer’s instructions. Briefly, HEK 293 cells and either DMSO

(17) Positive Allosteric Modulator of SERCA Pump NDC-1173 Exerts Beneficial Effects in Mouse Model of Alzheimer’s DiseaseInternational Journal of Molecular SciencesJuly 4, 2023Russell Dahl, Amanda C. Moore, Caitlynn Knight, Colleen Mauger, Hua Zhang, Gary E. Schiltz, Wendy A. Koss, Ilya Bezprozvannyassays in mouse liver microsomes were carried out using the ATPase Assay Kit (Colorimetric; Cat. no. ab234055; Abcam, Cambridge, UK) as per the manufacturer’s instructions. Briefly, microsomes and either DMSO control

(18) Corneal Edema in Inducible Slc4a11 Knockout Is Initiated by Mitochondrial Superoxide Induced Src Kinase ActivationCellsJune 1, 2023Ogando DG, Kim ET, Li S, Bonanno JA.same mouse were pooled and homogenized in 30 µL assay buffer, provided in the ATPase Assay kit (Abcam #ab234055), using a plastic disposable pestle. After sonication, the sample was centrifugated at 10,000× g for

(19) M2 macrophage-derived exosomes suppress tumor intrinsic immunogenicity to confer immunotherapy resistanceOncoimmunologyMay 13, 2023Naisheng Zheng, Tingting Wang, Qin Luo, Yi Liu, Junyao Yang, Yunlan Zhou, Guohua Xie, Yanhui Ma, Xiangliang Yuan, Lisong Shenin Supplementary Table S8. ATPase assay ATPase Assay Colorimetric kits were used for experiments (ab234055, Abcam). Briefly, reaction samples were prepared for each 10 min time point between 0 and 60 min. BiP

(20) A Novel XRE-Type Regulator Mediates Phage Lytic Development and Multiple Host Metabolic Processes in Pseudomonas aeruginosaMicrobiology SpectrumDecember 21, 2022Xiang Long, Xiaolong Wang, Daqing Mao, Weihui Wu, Yi Luodescribed above for the SPD transport experiment. An ATPase assay kit (colorimetric, catalog number ab234055; Abcam) was used to monitor ATPase activity in the cell extracts. Galleria mellonella-killing assay. A

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ATPase Assay Kit (Colorimetric) from Abcam

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ATPase Assay Kit (Colorimetric) from Abcam

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