Fig 1: Identification of discriminatory taxa and functional and associative patterns of the gut microbiota following serine supplementation in D-Gal-induced weaned piglets. (A and B) Linear discriminant analysis effect size (LEfSe) analysis showing differentially enriched taxa between groups (comparisons as indicated). (C) Prediction and analysis of microbial function in D-Gal group and Ser group. The color scale indicates the normalized relative level of each predicted function/phenotype (low to high, as shown in the legend). (D) Correlations among the gut microbiota and the indices of immunity, and serum biochemical indicators. The color scale represents the correlation coefficient from negative to positive, and deeper color intensity indicates a stronger association. Ctrl, basal diet (control); D-Gal, basal diet + D-galactose; Ser, basal diet + 1.00% serine + D-galactose. SIgA = secretory immunoglobulin A; SIgG = secretory immunoglobulin G; SIgM = secretory immunoglobulin M; TP = total protein; ALB = albumin; CREA = creatinine. ACE = abundance-based coverage estimator; Ctrl = control; D-Gal = D-galactose. ∗ P < 0.05 and ∗∗ P < 0.01 (n = 7).