DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) ab238535 from Abcam

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ItemDCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates)
CompanyAbcam
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Catalog Numberab238535
Quantity96 Test

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Abcam

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(1) SRPX2 exhibits neuroprotective effects in neural stem cells: inhibition of OGD/R-stimulated apoptosis and oxidative stressOpen Life SciencesJanuary 23, 2026Pan Li, Jie Huafixed and blocked with goat serum for 1 h. Slices were further incubated with DCF/ROS detection kit (ab238535, Abcam) or beta-catenin (1:200, ab32572), SRPX2 (1:1,000, ab91584, abcam), under the manufacture’s guidelines.

(2) Dual targeting of iNOS and Src tyrosine kinase as a superior therapeutic strategy against soman-induced long-term neurotoxicity: multimodal biomarker, imaging, and neurobehavioral outcome analysesActa Neuropathologica CommunicationsJanuary 9, 2026Nyzil Massey, Suraj S. Vasanthi, Christina Meyer, Nikhil S. Rao, Daniel R. Thedens, Chong Wang, Sridhar Kannurpatti, Thimmasettappa Thippeswamy(ROS) levels were measured using the dichlorodihydrofluorescein (DCF) ROS/RNS Assay Kit (Abcam; Cat. #ab238535) according to the manufacturer’s instructions. Serum samples were centrifuged at 10,000 × g for 5 min

(3) Mitochondrial DNA‐Mediated Immune Activation After Resuscitation From Cardiac ArrestJournal of the American Heart AssociationJanuary 6, 2026Tyler J. Rolland, Emily R. Hudson, Luke A. Graser, Sumbule Zahra, Daniel Cucinotta, Swati D. Sonkawade, Umesh C. Sharma, Brian R. Weilmedia levels of reactive oxygen and nitrogen species were measured using a DCF‐based kit (Abcam, Ref: ab238535), and intracellular levels of 2′3′‐cyclic guanosine monophosphate–adenosine monophosphate (cGAMP; Invitrogen,

(4) Cannabidiol Mitigates Pollution-Induced Inflammatory, Oxidative, and Barrier Damage in Ex Vivo Human SkinBiomoleculesDecember 20, 2025Wannita Klinngam, Orathai Loruthai, Sornkanok Vimolmangkangan EnVision-Alpha plate reader (Revvity, USA). ROS were quantified using the DCF ROS/RNS Assay Kit (#ab238535; Abcam, Cambridge, MA, USA). Oxidative DNA damage marker 8-OHdG and PIP were measured by ELISA using

(5) Illuminating the Underlying Mechanism of Intracellular Optoelectronic Modulation Using Silicon NanowiresACS NanoOctober 28, 2025Tania Assaf, Layan Habib, Adi Sasson, Shiri Karni-Ashkenazi, Menahem Y. Rotenberg2-APB, with a 40 min incubation prior to imaging. Total ROS Detection The DCF ROS/RNS Detection Kit (ab238535, Abcam) was used to assess reactive species production. SiNWs were added to the imaging medium of cultured cells

(6) Exploring multiorgan mitochondrial dysfunction in the switch toward progressive MASLD in AMLN miceiScienceSeptember 19, 2025Meroni M, Paolini E, Longo M, Battistin M, Dondossola D, Ripolone M, Napoli L, Mosca E, Corti S, Dongiovanni P.and Hydrogen Peroxide concentrations in tissue lysates, following the manufacturer’s instructions (ab238535 and ab102500, respectively, Abcam). Lipid peroxidation was quantified by the measure of reactive aldehydic

(7) Mitigating organophosphate nerve agent, soman (GD), induced long-term neurotoxicity: Saracatinib, a Src Tyrosine Kinase inhibitor, as a potential countermeasureJ NeuroinflammationAugust 5, 2025Nyzil Massey, Suraj S. Vasanthi, Claire Holtkamp, Christina Meyer, Nikhil S. Rao, Luis G. Gimenez-Lirola, Chong Wang, Hyunmook Im, Avinash S. Bevoor, Sridhar Kannurpatti, et al.samples were measured using a dichlorodihydrofluorescein (DCF) ROS/RNS Assay Kit (Abcam; catalog # ab238535), following the manufacturer's instructions. Fluorescence intensity was quantified at an excitation

(8) Splenic modulation of the early inflammatory response to regional and global ischemia/reperfusion injury in swineAmerican Journal of Physiology-Heart and Circulatory PhysiologyJuly 1, 2025Tyler J. Rolland, Emily R. Hudson, Luke A. Graser, Sumbule Zahra, Daniel Cucinotta, Brian R. Weilplasma levels of reactive oxygen and nitrogen species (ROS/RNS) were measured using a DCF-based kit (ab238535, Abcam, Cambridge, UK) following the manufacturer’s recommendations and as previously described.43,44 Statistical

(9) Kurarinone activates the Nrf-2/HO-1 signaling pathway and alleviates high glucose-induced ferroptosis in HK2 cellsJournal of Clinical Biochemistry and NutritionApril 16, 2025Chunmei Mawell as blocked with goat serum for 1 ‍h. Slices were further incubated with DCF/ROS detection kit (ab238535; Abcam, Cambridge, UK) according to the manufacturer’s guidelines. FCM detection For the FCM detection

(10) Proinflammatory cytokines, oxidative stress, and organ function as biomarkers of soman (GD) chronic neurotoxicitySci RepMarch 15, 2025Nyzil Massey, Suraj S. Vasanthi, Luis G. Gimenez-Lirola, Harm Tyler, Thimmasettappa Thippeswamy(DCF) ROS/RNS Assay Kit, suitable for biofluids, culture supernatant, and cell lysates (Abcam; Catalog#ab238535), was used to measure the levels of reactive oxygen species (ROS) in serum samples. The assay was conducted

(11) Quantification of Total Free Radicals in Drosophila Using a Fluorescence-Based Biochemical AssayBIO-PROTOCOLMarch 5, 2025Shahira Helal Arzoo, Rubaia Tasmin, Surya Jyoti Banerjeefrom Bloomington Drosophila stock center) Reagents 1. DCF ROS/RNS assay kit (Abcam, catalog number: ab238535); contains enough reagents to perform 96 assays (Table 1) 2. Bovine serum albumin (BSA) (Sigma-Aldrich,

(12) MANF facilitates breast cancer cell survival under glucose-starvation conditions via PRKN-mediated mitophagy regulationAutophagyJanuary 2, 2025Zhenchong Xiong, Lin Yang, Chao Zhang, Weiling Huang, Wenjing Zhong, Jiarong Yi, Jikun Feng, Xiazi Zouxu, Libing Song, Xi Wangand weights. ROS levels in fresh tumor tissues were detected using a DCF ROS/RNS Assay Kit (Abcam, ab238535). A 50-mg tissue sample was homogenized in 1 mL of PBS on ice. The obtained homogenate was then centrifuged

(13) Skeletal muscle reprogramming enhances reinnervation after peripheral nerve injuryNature CommunicationsOctober 25, 2024Pihu Mehrotra, James Jablonski, John Toftegaard, Yali Zhang, Shahryar Shahini, Jianmin Wang, Carey W. Hung, Reilly Ellis, Gabriella Kayal, Nika Rajabian, et al.Total free radical presence in 5 µg of each sample was measured using the DCF ROS/RNS Assay Kit (Cat # ab238535, Abcam) as per manufacturer recommended protocol. RNA sequencing and pathway analysis The global gene expression

(14) Early oxidative stress and DNA damage in Aβ-burdened hippocampal neurons in an Alzheimer's-like transgenic rat modelCommunications biologyJuly 14, 2024Foret MK, Orciani C, Welikovitch LA, Huang C, Cuello AC, Do Carmo S.{factor}={mU}/{mL}$$\end{document}GRActivity=ΔBT1−T2×0.9×V×Sampledilutionfactor=mU/mL DCF assay The DCF ROS/RNS fluorogenic Assays (Abcam, ab238535) was used to determine the levels of ROS and RNS by measuring the fluorescence intensity in cortical

(15) Skin Rejuvenation Efficacy and Safety Evaluation of Kaempferia parviflora Standardized Extract (BG100) in Human 3D Skin Models and Clinical TrialBiomoleculesJune 29, 2024Wannita Klinngam, Phetploy Rungkamoltip, Ratjika Wongwanakul, Jaruwan Joothamongkhon, Sakkarin Du-a-man, Mattaka Khongkow, Udom Asawapirom, Tawin Iempridee, Uracha RuktanonchaiSupernatants were collected, and ROS and 8-OHdG levels were measured using the DCF ROS/RNS Assay Kit (#ab238535, Abcam, Cambridge, MA, USA) and 8-hydroxy 2-deoxyguanosine ELISA Kit (#ab201734, Abcam, Cambridge, MA,

(16) Loranthus tanakae Franch. and Sav. Attenuates Respiratory Inflammation Caused by Asian Sand DustAntioxidants (Basel)March 29, 2024Se-Jin Lee, So-Won Pak, A Yeong Lee, Woong-Il Kim, Sung-Wook Chae, Young-Kwon Cho, Je-Won Ko, Tae-Won Kim, Jong-Choon Kim, Byeong Cheol Moon, et al.Species (ROS) For ROS detection in BALF, a 2′, 7′-dichlorodihydrofluorescein (DCF) ROS/RNS assay kit (ab238535, Abcam) was performed according to the protocol of manufacturer. For intracellular ROS levels, NCI-H292

(17) Rhenium(I) Tricarbonyl Complexes of 1,10-Phenanthroline Derivatives with Unexpectedly High CytotoxicityInorganic ChemistryAugust 7, 2023Lucy E. Enslin, Kallol Purkait, Maria Dalla Pozza, Bruno Saubamea, Pierre Mesdom, Hendrik G. Visser, Gilles Gasser, Marietjie Schutte-Smithtreat the cells for 30 min (10 and 15 μM). The medium was removed, and 40 μM DCFH-DiOxy-Q (Kit No. ab238535, Abcam) in 10% FBS containing HEPES was added in each well followed by incubation at 37 °C for 30 min.

(18) Riluzole, a Derivative of Benzothiazole as a Potential Anti-Amoebic Agent against Entamoeba histolyticaPharmaceuticals (Basel)June 19, 2023Maritza Velásquez-Torres, José Guadalupe Trujillo-Ferrara, Marycarmen Godínez-Victoria, Rosa Adriana Jarillo-Luna, Víctor Tsutsumi, Virginia Sánchez-Monroy, Araceli Posadas-Mondragón, Roberto Issac Cuevas-Hernández, José Angel Santiago-Cruz, Judith Pacheco-YépezROS production was determined by employing a 2′, 7′-dichlorodihydrofluorescein (DCF) ROS assay kit (Ab238535, Abcam, Cambridge, UK). ROS production was quantified in trophozoites cultured in 3 × 104 cells of fresh

(19) Inhibition of glutaminolysis restores mitochondrial function in senescent stem cellsCell reportsNovember 29, 2022Choudhury D, Rong N, Ikhapoh I, Rajabian N, Tseropoulos G, Wu Y, Mehrotra P, Thiyagarajan R, Shahini A, Seldeen KL, et al.ab65342 Ammonia Assay Kit,Abcam,Cat# ab83360 Urea Assay Kit,Abcam,Cat# ab83362 DCF ROS/RNS Assay Kit,Abcam,Cat# ab238535 Senescence Detection Kit,Abcam,Cat# ab65351 Experimental models: Cell lines,Experimental models: Cell

(20) Fenugreek Seed Galactomannan Aqueous and Extract Protects against Diabetic Nephropathy and Liver Damage by Targeting NF-κB and Keap1/Nrf2 AxisToxicsJune 30, 2022Alsuliam SM, Albadr NA, Almaiman SA, Al-Khalifah AS, Alkhaldy NS, Alshammari GM.rat-specific assay or ELISA kits. The levels of ROS/RNS in tissue lysate were measured using a DCF-based kit (#ab238535; Abcam, Cambridge, CB2 0AX, UK) and as previously used by others [42,43,44]. Homogenates levels of malondialdehyde

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DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) from Abcam

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DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates) from Abcam

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