TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 from Abcam

Product Specs
ItemTMRM Assay Kit (Mitochondrial Membrane Potential)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228569
Sample TypeAdherent cells, Suspension cells
Quantity1 Kit

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Abcam

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Cambridge CB2 0AX

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Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) SGLT2 Inhibitor Dapagliflozin Attenuates Cardiomyocyte Injury and Inflammation Induced by PI3Kα-Selective Inhibitor Alpelisib and Fulvestrant Under HyperglycemiaInt J Mol SciApril 17, 2026Vincenzo Quagliariello, Massimiliano Berretta, Matteo Barbato, Fabrizio Maurea, Maria Laura Canale, Andrea Paccone, Irma Bisceglia, Andrea Tedeschi, Marino Scherillo, Jacopo Santagata, et al.assessed using the potentiometric dye tetramethylrhodamine methyl ester (TMRM) in non-quench mode (Cat. N ab228569; AbCam, Milan, Italy). TMRM accumulates in mitochondria in a membrane potential-dependent manner and

(2) An iPSC-derived neuronal model reveals manganese’s role in neuronal endocytosis, calcium flux and mitochondrial bioenergeticsiScienceSeptember 19, 2025Dimitri Budinger, Sharmin Alhaque, Ramón González-Méndez, Chris Dadswell, Katy Barwick, Arianna Ferrini, Charlotte Roth, Conor J. McCann, Karin Tuschl, Fatma Al Jasmi, et al.DNase I,Invitrogen,Cat#18068015 SuperScript II Reverse Transcriptase,Invitrogen,Cat#18064014 TMRM assay kit,Abcam,Cat#ab228569 FM 1-43FX,Invitrogen,Cat#F35355 Alt-R® S.p. HiFi Cas9 Nuclease V3,Integrated DNA Technologies,Cat#1081061 Alt-R™ HDR Enhancer V2

(3) A sesquiterpene lactone, tomentosin, as a novel anticancer agent: orchestrating apoptosis, autophagy, and ER stress in colorectal cancerNaunyn-Schmiedeberg's Archives of PharmacologySeptember 1, 2025Sümeyra Çetinkaya, Ebru Güçlü, İlknur Çınar Ayan, Hasibe Vural, Hatice Gül Dursunwas evaluated to determine whether it triggers mitochondrial dysfunction. For this, the TMRM assay (ab228569, Abcam) was performed according to the manufacturer’s instructions. Briefly, TMRM working solution was

(4) Uric Acid, the End-Product of Purine Metabolism, Mitigates Tau-Related Abnormalities: Comparison with DOT, a Non-Antibiotic Oxytetracycline DerivativeBiomoleculesJune 28, 2025Bianca Andretto de Mattos, Rodrigo Hernán Tomas-Grau, Thaís Antonia Alves Fernandes, Florencia González-Lizárraga, Aurore Tourville, Ismaila Ciss, Jean-Michel Brunel, Rosana Chehin, Annie Lannuzel, Laurent Ferrié, et al.mM). Then, the cultures were exposed to tetramethylrhodamine methyl ester, perchlorate (TMRM; 50 nM; ab228569; Abcam, Cambridge, UK), and dihydrorhodamine 123 (DHR-123; 25 µM; D23806; Thermo Fisher Scientific)

(5) An acute microglial metabolic response controls metabolism and improves memoryeLifeDecember 3, 2024Anne Drougard, Eric H Ma, Vanessa Wegert, Ryan Sheldon, Ilaria Panzeri, Naman Vatsa, Stefanos Apostle, Luca Fagnocchi, Judith Schaf, Klaus Gossens, et al.suspensions were incubated with the mitochondrial probe tetramethylrhodamine TMRM 10 mM (Abcam, Cat# ab228569) for 30 min at 37°C before FACS acquisition. For challenging the ETC, the cell pellet was resuspended

(6) EPRS1-mediated fibroblast activation and mitochondrial dysfunction promote kidney fibrosisExperimental & Molecular MedicineDecember 2, 2024Seung Seob Son, Hee Seul Jeong, Seong-Woo Lee, Eun Soo Lee, Jeong Geon Lee, Ji-Hye Lee, Jawoon Yi, Mi Ju Park, Min Sun Choi, Donghyeong Lee, et al.total protein concentration of each sample. To assess mitochondrial function in HK-2 cells, a TMRM kit (ab228569; Abcam) was used according to the manufacturer’s instructions. Hoechst 33342 (62249, Thermo Fisher Scientific)

(7) TXNDC5 Plays a Crucial Role in Regulating Endoplasmic Reticulum Activity through Different ER Stress Signaling Pathways in Hepatic CellsInternational Journal of Molecular SciencesJune 28, 2024Seyed Hesamoddin Bidooki, Cristina Barranquero, Javier Sánchez-Marco, Roberto Martínez-Beamonte, María J. Rodríguez-Yoldi, María A. Navarro, Susana C. M. Fernandes, Jesús Osadarespectively. 4.13. Mitochondrial Membrane Potential Assay The mitochondrial membrane potential assay (Ab228569 TMRM assay kit, Abcam, Waltham, MA, USA) was used to determine potential mitochondrial damage caused

(8) RAB7 deficiency impairs pulmonary artery endothelial function and promotes pulmonary hypertensionJournal of Clinical InvestigationFebruary 1, 2024Bryce Piper, Srimathi Bogamuwa, Tanvir Hossain, Daniela Farkas, Lorena Rosas, Adam C. Green, Geoffrey Newcomb, Nuo Sun, Jose A. Ovando-Ricardez, Jeffrey C. Horowitz, et al.cells (decreased ΔΨm). ΔΨm distribution was also determined using TMRM staining (TMRM Assay Kit, Abcam, ab228569) on adherent PAECs followed by nuclear staining with Hoechst 33342 (Thermo Fisher Scientific, H-1399).

(9) Mitochondrial Stress Links Environmental Triggers with Pro-Inflammatory Signaling in Crohn's DiseaseAntioxidants (Basel)December 13, 2023Flores Martín-Reyes, Manuel Bernal, Cristina Rodríguez-Díaz, Damaris Rodríguez-de Los Reyes, Ailec Ho-Plagaro, Francisca Rodríguez-Pacheco, Laura Camacho-Martel, Raquel Camargo-Camero, Francisco J. Rodríguez-González, Guillermo Alcain-Martínez, et al.for three weeks, and stained with 800 nM tetramethylrhodamine methyl ester (TMRM) (TMRM Assay Kit, ab228569, Abcam, Cambridge, UK) for assessment of mitochondrial membrane potential or 5 µM dihydroethidine (DHE)

(10) The Pesticide Chlordecone Promotes Parkinsonism-like Neurodegeneration with Tau Lesions in Midbrain Cultures and C. elegans WormsCellsMay 7, 2023Valeria Parrales-Macias, Patrick P. Michel, Aurore Tourville, Rita Raisman-Vozari, Stéphane Haïk, Stéphane Hunot, Nicolas Bizat, Annie Lannuzelsupplemented with glucose (5 mM). Thereafter, the cultures were exposed sequentially to the mitoprobe TMRM (ab228569; Abcam, Cambridge, UK) at 50 nM and the ROS indicator DHR-123 (D23806; Thermo Fisher Scientific) at

(11) A rapid microglial metabolic response controls metabolism and improves memorybioRxivApril 11, 2023Anne Drougard, Eric H Ma, Vanessa Wegert, Ryan Sheldon, Ilaria Panzeri, Naman Vatsa, Stefanos Apostle, Luca Fagnocchi, Judith Schaf, Klaus Gossens, et al.the cell suspension was incubated with the mitochondrial probe Tetramethylrhodamine TMRM (Abcam, Cat# ab228569) and fluorescent glucose analog 2-NBDG (Abcam, Cat# 235976) for 30min at 37degrees before FACS acquisition.

(12) Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 CellsAntioxidants (Basel)March 27, 2023Estefanía Caballano-Infantes, Ailec Ho-Plágaro, Carlos López-Gómez, Flores Martín-Reyes, Francisca Rodríguez-Pacheco, Bernard Taminiau, Georges Daube, Lourdes Garrido-Sánchez, Guillermo Alcaín-Martínez, Raúl J. Andrade, et al.tetramethylrhodamine methyl ester (TMRM) dye in live cells (TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569, Abcam, Cambridge, UK). Briefly, after incubation with MVs in a 96 wells microplate, HepG2 cells (2

(13) Morphological and mitochondrial changes in murine choroid plexus epithelial cells during healthy agingFluids and barriers of the CNSMarch 14, 2023Scarpetta V, Bodaleo F, Salio C, Agarwal A, Sassoè-Pognetto M, Patrizi A.or with10 µM Oligomycin A (HB4488, Hellobio) for 15 min, followed by co-incubation with 25 nM TMRM (ab228569, Abcam) and 100 nM MitoTracker Green (M7514, Thermofisher) for 45 min. For mitochondrial reactive oxygen

(14) Rescue of Dopamine Neurons from Iron-Dependent Ferroptosis by Doxycycline and Demeclocycline and Their Non-Antibiotic DerivativesAntioxidants (Basel)February 24, 2023Aurore Tourville, Sarah Viguier, Florencia González-Lizárraga, Rodrigo Hernán Tomas-Grau, Paola Ramirez, Jean-Michel Brunel, Mauricio Dos Santos Pereira, Elaine Del-Bel, Rosana Chehin, Laurent Ferrié, et al.cultures were then exposed for 35 min to tetramethylrhodamine methyl ester, perchlorate (TMRM; 50 nM; ab228569; Abcam, Cambridge, UK) together with dihydrorhodamine 123 (DHR-123; 25 µM; D23806; Thermo Fisher Scientific)

(15) A trans-kingdom T6SS effector induces the fragmentation of the mitochondrial network and activates innate immune receptor NLRX1 to promote infectionNature communicationsFebruary 16, 2023Sá-Pessoa J, López-Montesino S, Przybyszewska K, Rodríguez-Escudero I, Marshall H, Ova A, Schroeder GN, Barabas P, Molina M, Curtis T, et al.enterocolitica strains. Mitochondrial Membrane Potential was assessed with a TMRM assay kit (Abcam, ab228569) following manufacturer’s instructions. Briefly TMRM was added to the culture media and incubated for

(16) Inhibition of autophagy and MEK promotes ferroptosis in Lkb1-deficient Kras-driven lung tumorsCell death & diseaseJanuary 26, 2023Bhatt V, Lan T, Wang W, Kong J, Lopes EC, Wang J, Khayati K, Raju A, Rangel M, Lopez E, et al.(Cat#: M7514) were purchased from Invitrogen. TMRM Assay Kit (Mitochondrial Membrane Potential) (Cat#: ab228569) was purchased from Abcam. Tumor burden quantification Hematoxylin and eosin (H&E)-stained lung specimens

(17) Mitochondrial Dysfunction Associates With Acute T Lymphocytopenia and Impaired Functionality in COVID-19 PatientsFrontiers in immunologyJanuary 1, 2021Mo Y, To KK, Zhou R, Liu L, Cao T, Huang H, Du Z, Lim CYH, Yim LY, Luk TY, et al.,Thermo Fisher,Cat. No: M7514 MitoTracker™ Red CMXRos,Thermo Fisher,Cat. No: M7512 TMRM assay kit,Abcam,Cat. No: ab228569 MitoSOX™ Red Mitochondrial Superoxide Indicator,Thermo Fisher,Cat. No: M36008 "Fluo-4FF, AM, cell

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TMRM Assay Kit (Mitochondrial Membrane Potential) from Abcam

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TMRM Assay Kit (Mitochondrial Membrane Potential) from Abcam

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