Protein Synthesis Assay Kit (Green) ab239725 from Abcam

Product Specs
ItemProtein Synthesis Assay Kit (Green)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab239725
Quantity100 Test
Sample TypeAdherent cells, Suspension cells

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) 8-Me-PIQ Expands Cord Blood CD34+ Hematopoietic Stem and Progenitor Cells via Metabolic Suppression and Inhibition of Ribosome BiogenesisStem Cell Reviews and ReportsApril 17, 2026Yu Li, Mengmeng Li, Bin Guo, Rongzhen Jiang(O-propargyl-puromycin, OP-puro) Protein synthesis rates were measured using the Protein Synthesis Assay Kit (Abcam, ab239725) according to the manufacturer’s instructions. Briefly, cells were resuspended in culture medium, and

(2) Age-related adiponectin resistance in human skeletal muscle dysfunction: in vivo and in vitro evidenceJournal of Translational MedicineMarch 5, 2026Surina Surina, Lucia Scisciola, Manuela Giovanna Basilicata, Ada Pesapane, Rosaria Anna Fontanella, Nunzia Balzano, Alberta Maria Maddalena Palazzo, Asad Zia, Giovanni Tortorella, Zeeshan Ulfat, et al.conducted according to the manufacturer’s instructions for the protein synthesis assay kit (Abcam, ab239725), and the measurements were carried out using a flow cytometer. Lipid droplet detection assay Cells

(3) Activating GCN2 and subsequently the Unfolded Protein Response with the small oral molecule NXP800 delays tumor growth in osteosarcomaCell Death DiscovFebruary 5, 2026Emma Racineau, Morgane Lallier, Anaïs Postec, Jérôme Amiaud, Rose-Anne Thépault, Régis Brion, Séverine Battaglia, Céline Charrier, Marie-Anne Colle, Bénédicte Brounais-Le Royer, et al.Beta-2-Microglobulin (B2M). The list of primers used is detailed in Table S3. OPP Protein Synthesis Assay Kit (ab239725, Abcam) was used for the detection of global nascent proteins of OS cell lines following the instructions

(4) TGFβ pathway represses hepatic ribosome biogenesis and protein synthesis by regulating p70S6K–S6RP proteinsCellular & Molecular Biology LettersJanuary 16, 2026Athanasios Stavropoulos, Vassiliki Stamatopoulou, Eleftherios Pavlos, Maria Manioudaki, Stratigoula Sakellariou, Constantinos Stathopoulos, Maria XilouriTable S2. Protein synthesis in Hepa1-6 cells was assessed using the Protein Synthesis Assay Kit (Abcam, ab239725), which employs the O-propargyl-puromycin (OP-puro) analog to label nascent polypeptides. Incorporated

(5) Paclitaxel-Loaded Polyelectrolyte Nanocarriers: Uptake Mechanisms, Cytotoxicity, and Genotoxicity in Human Endothelial and Breast Cancer CellsNanotechnol Sci ApplDecember 24, 2025Marzena Szwed, Anastazja Poczta-Krawczyk, Katarzyna Dominika Kania, Karol Bukowski, Katarzyna Bednarska-Szczepaniak, Agnieszka Marczak, Krzysztof SzczepanowiczCellTiter-Glo® Luminescent Cell Viability Assay (Promega, Madison, WI, USA), the Protein Synthesis Assay Kit (Green, ab239725; Abcam, Cambridge, UK), the MycoAlert™ Mycoplasma Detection Kit (Lonza, Walkersville, MD, USA), SYBR

(6) Paclitaxel-Loaded Polyelectrolyte Nanocarriers: Uptake Mechanisms, Cytotoxicity, and Genotoxicity in Human Endothelial and Breast Cancer CellsNanotechnol Sci ApplDecember 24, 2025Marzena Szwed, Anastazja Poczta-Krawczyk, Katarzyna Dominika Kania, Karol Bukowski, Katarzyna Bednarska-Szczepaniak, Agnieszka Marczak, Krzysztof SzczepanowiczCellTiter-Glo® Luminescent Cell Viability Assay (Promega, Madison, WI, USA), the Protein Synthesis Assay Kit (Green, ab239725; Abcam, Cambridge, UK), the MycoAlert™ Mycoplasma Detection Kit (Lonza, Walkersville, MD, USA), SYBR

(7) Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signalingNat CommunOctober 8, 2025Jung Seok Hwang, JiHoon Kang, Jaehyun Kim, Kiyoung Eun, Sophia West, Hannah E. Bacho, Vanessa Avalos, Sydney Shuff, Dong M. Shin, Nabil F. Saba, et al.(ab109902), EZClick Global RNA Synthesis assay (ab228561), and EZClick Global Protein Synthesis assay (ab239725) were purchased from Abcam. High-Capacity cDNA Reverse Transcription Kit (4374966) was from Applied Biosystems

(8) Ribosome biogenesis is essential for hemogenic endothelial cells to generate hematopoietic stem cellsDevelopmentNovember 1, 2024Di Liu, Haizhen Wang, Haifeng Chen, Xitong Tian, Yuqing Jiao, Chi Wang, Yuhui Li, Zongcheng Li, Siyuan Hou, Yanli Ni, et al.potential. Protein synthesis assay (O-propargyl-puromycin, OP-puro) The Protein Synthesis Assay Kit (Abcam, ab239725) was used to measure translation rates in AECs, HECs and pre-HSCs. Briefly, E10.5 caudal half and E11.0

(9) Proinflammatory phenotype of iPS cell-derived JAK2 V617F megakaryocytes induces fibrosis in 3D in vitro bone marrow nicheStem Cell ReportsFebruary 13, 2024Niclas Flosdorf, Janik Böhnke, Marcelo A.S. de Toledo, Niklas Lutterbach, Vanesa Gómez Lerma, Martin Graßhoff, Kathrin Olschok, Siddharth Gupta, Vithurithra Tharmapalan, Susanne Schmitz, et al.NJ). For the analysis of protein biosynthesis with flow cytometry, the protein synthesis assay kit (ab239725, Abcam, Cambridge, UK) was used. Diff-Quik staining cells were centrifuged onto glass slides in the

(10) High content screening and proteomic analysis identify a kinase inhibitor that rescues pathological phenotypes in a patient-derived model of Parkinson’s diseasenpj Parkinson's DiseaseDecember 1, 2022Nasia Antoniou et al.Scientific, Inc.). Protein synthesis assay For detection of total protein synthesis, an assay Kit (ab239725; Abcam) was used that utilizes a cell permeable analog of puromycin, which once inside the cell, stops

(11) Matrine, a potential c-Myc inhibitor, suppresses ribosome biogenesis and nucleotide metabolism in myeloid leukemiaFrontiers in pharmacologyJanuary 1, 2022Zhong WJ, Ma L, Yang F, Cao J, Tan J, Li B.gRNAs are in Supplementary Table S11. Global nascent proteins detection Protein Synthesis Assay Kit (ab239725, Abcam, UK) was used for the detection of global nascent proteins of leukemia cells following the instructions

(12) Ronin governs the metabolic capacity of the embryonic lineage for post-implantation developmentEMBO reportsNovember 4, 2021Salewskij K, Gross-Thebing T, Ing-Simmons E, Duethorn B, Rieger B, Fan R, Chen R, Govindasamy N, Brinkmann H, Kremer L, et al.instructions. Flowjo software (TreeStar) was used for data analysis. OP‐puro assay The OP‐puro assay (Abcam, ab239725) was carried out following the manufacturer’s instructions. TMRE assay The cells were stained with

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Protein Synthesis Assay Kit (Green) from Abcam

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Protein Synthesis Assay Kit (Green) from Abcam

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