Beta Galactosidase Assay Kit (Senescence) ab228562 from Abcam

Product Specs
ItemBeta Galactosidase Assay Kit (Senescence)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228562
Quantity100 Test
Sample TypeAdherent cells, Suspension cells
ApplicationsEnzyme activity assay
Research AreaOncology
NCBI Full Gene Namegalactosidase beta 1
NCBI Gene AliasesEBP, ELNR1, MPS4B

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Website: http://www.abcam.com

(1) Targeting oncogene-induced senescence in ETV6::RUNX1 pre-leukemic cellsCell Death DiscoveryMarch 11, 2026Denise Acunzo, Mayla Bertagna, Giulia Risca, Linda Beneforti, Silvia Bresolin, Stefania Galimberti, Isidro Sánchez-García, Andrea Biondi, Giovanni Cazzaniga, Chiara Palmiflow cytometric analysis of senescence-associated β-Galactosidase (SA β-Gal) activity, we used the Senescence assay Kit (ab228562, Abcam) according to the manufacturer’s instructions. Briefly, after Lin- enrichment, the cells were

(2) Nrf2/Nlrp3 signaling in aging BMSCs: Traf6 intervention as a novel approach to osteoporosis treatmentRedox BiologyOctober 1, 2025Yajun Li, Yunshang Yang, Donglong Xia, Yiling Fang, Cheng Tang, Jingxian Yu, Dechun Geng, Zhirong Wang, Long Xiaothe medium was removed and replaced with 500 μL of fresh medium containing 1.5 μL of senescence dye (ab228562, Abcam) and incubated for 1–2 h at 37 °C in a constant temperature oven protected from light. After

(3) Heterochronic parabiosis uncovers AdipoR1 as a critical player in retinal rejuvenationScience advancesJuly 18, 2025Liu Y, Liu X, Lv J, Zhang Q, Yang Z, Chen X, Gu C, Zhang C, Zhuo Y, Su W.(no. 4414S, Cell Signaling Technology, Danvers, US). Cells were stained with the senescence assay (no. ab228562, Abcam) for β-GAL staining, following the manufacturer’s instructions. To assess mitochondrial ROS production

(4) Single-cell profiling unveils a geroprotective role of Procyanidin C1 in hematopoietic immune system via senolytic and senomorphic effectsNPJ AgingMay 2, 2025Xiuxing Liu, Yidan Liu, Yuehan Gao, Chun Zhang, Chenyang Gu, Jianjie Lv, Junying Wu, Wenru Suinstructions. Cells were collected for FCM analysis after being stained with the senescence assay kit (#ab228562, Abcam) for β-galactosidase (β-Gal) according to the manufacturer’s guidelines. For the apoptosis assay,

(5) Age‐Dependent Regulation of Hippocampal Inflammation by the Mitochondrial Translocator Protein in MiceAging CellApril 24, 2025Kei Onn Lai, Jia Hui Wong, Nevin Tham, Lauren Fairley, Roshan Ratnakar Naik, Yulan Wang, Sarah R. Langley, Anna M. BarronTo measure SA‐β‐Gal activity, cells were washed with PBS and incubated with senescence dye (Abcam, ab228562) for at 37°C 1 h, followed by two washes with assay buffer. Cells were fixed with 4% PFA for 10 min

(6) Pyrroloquinoline Quinone Reprograms the Single‐Cell Landscape of Immune Aging in Hematopoietic Immune SystemAging CellApril 7, 2025Xiuxing Liu, Chun Zhang, Jianjie Lv, Yidan Liu, Chenyang Gu, Yuehan Gao, Wen Ding, Hui Chen, Nanwei Xu, Hongbin Yin, et al.analysis. For the β‐galactosidase (β‐Gal) staining, cells were stained with the senescence assay kit (#ab228562, Abcam) and harvested for FCM analysis according to the manufacturer's instructions. For Annexin V and

(7) Insulin-Like Growth Factor 2 Secreted from Mesenchymal Stem Cells with High Glutathione Levels Alleviates Osteoarthritis via Paracrine Rejuvenation of Senescent ChondrocytesBiomater ResFebruary 21, 2025Gun Hee Cho, Hyun Cheol Bae, Yu Jeong Lee, Ha Ru Yang, Hyewon Kang, Hee Jung Park, Sun Young Wang, You Jung Kim, Heun-Soo Kang, In Gyu Kim, et al.senescence-associated β-galactosidase (SA-β-Gal) kit was used to evaluate SA-β-Gal activity (Abcam, ab228562) according to the manufacturer’s instructions with slight modifications. Briefly, chondrocytes were

(8) Glioblastoma progression is hindered by melatonin-primed mesenchymal stromal cells through dynamic intracellular and extracellular reorganizationsTheranosticsFebruary 10, 2025Laura Olmedo-Moreno, Concepción Panadero-Morón, Jesús María Sierra-Párraga, Rubén Bueno-Fernández, Emily S. Norton, Yolanda Aguilera, Nuria Mellado-Damas, Patricia García-Tárraga, Raquel Morales-Gallel, María Antequera-Martínez, et al.activity was detected on attached MSCs (treated or not with melatonin) using a Senescence Assay Kit (ab228562, Abcam, Cambridge, UK), according to manufacturer's protocol. After proper staining, cells were collected

(9) Unraveling the Role of RSPRY1 in TGF-β Pathway Dysregulation: Insights into the Pathogenesis of Spondyloepimetaphyseal DysplasiaInternational Journal of Molecular SciencesJanuary 28, 2025Gozde Imren, Beren Karaosmanoglu, Bihter Muratoglu, Cansu Ozdemir, Gulen Eda Utine, Pelin Ozlem Simsek-Kiper, Ekim Z. Taskiranrecognized marker of cellular senescence, was detected using a fluorescence-based substrate (cat. no. ab228562, Abcam, Cambridge, UK). The cells were incubated for 90 min at 5% CO2 and 37 °C, then washed with the

(10) Piezo1 exacerbates inflammation‐induced cartilaginous endplate degeneration by activating mitochondrial fission via the Ca2+/CaMKII/Drp1 axisAging CellNovember 28, 2024Zhidi Lin, Guangyu Xu, Xiao Lu, Hongli Wang, Feizhou Lu, Xinlei Xia, Jian Song, Jianyuan Jiang, Xiaosheng Ma, Fei Zoustaining Senescence‐associated β‐galactosidase staining was performed by using a Senescence Assay Kit (ab228562, Abcam). CEP cells were incubated with senescence working solution according to the manufacturer's protocol.

(11) Optimization of Antiproliferative Properties of Triimine Copper(II) ComplexesJournal of Medicinal ChemistryNovember 14, 2024Katarzyna Choroba, Bartosz Zowiślok, Sławomir Kula, Barbara Machura, Anna M. Maroń, Karol Erfurt, Cristiana Marques, Sandra Cordeiro, Pedro V. Baptista, Alexandra R. Fernandessoftware). Evaluation of Cellular Senescence In this assay, a senescence assay kit (Beta Galactosidase, Fluorescence) (ab228562; Abcam, UK) was used. HCT116-DoxR cells were seeded in 24-well plates at a density of 5 × 105 cells/well

(12) Microfragmented abdominal adipose tissue‐derived stem cells from knee osteoarthritis patients aged 29–65 years demonstrate in vitro stemness and low levels of cellular senescenceJournal of Experimental OrthopaedicsJuly 1, 2024Freja Aabæk Hammer, Per Hölmich, Jan O. Nehlin, Kilian Vomstein, Lars Blønd, Lisbet Rosenkrantz Hölmich, Kristoffer Weisskirchner Barfod, Jasmin Baggeactivity assay kit for flow cytometry modified according to the manufacturer's protocol (Abcam, Cat# ab228562). At 70% confluency, passage 3 cells cultured in T75 flasks were split to passage 4 and transferred

(13) Extracellular vesicles produced by irradiated endothelial or Glioblastoma stem cells promote tumor growth and vascularization modulating tumor microenvironmentCancer cell internationalFebruary 12, 2024Castellani G, Buccarelli M, D'Alessandris QG, Ilari R, Cappannini A, Pedini F, Boe A, Lulli V, Parolini I, Giannetti S, et al.chromogen. For the senescence detection by flow cytometry, a Senescence Assay Kit was purchased from Abcam (Ab228562, Abcam, Cambridge, UK) and the evaluation of β-gal activity in senescent cells were performed according

(14) SHP2 inhibitors maintain TGFβ signalling through SMURF2 inhibitionNPJ Precis OncolDecember 15, 2023Xianning Lai, Sarah Kit Leng Lui, Hiu Yan Lam, Yuta Adachi, Wen Jing Sim, Natali Vasilevski, Nicola J. Armstrong, Stephanie Claire Bridgeman, Nathan Michael Main, Tuan Zea Tan, et al.to the manufacturer’s protocol (Sigma-GALS). For fluorescent detection of Beta Galactosidase (Abcam ab228562), cells were treated as above and stained using the manufacturers protocols. Quantitative analysis was

(15) Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestionJournal of Experimental OrthopaedicsMarch 23, 2023Jasmin Bagge, Per Hölmich, Freja Aabæk Hammer, Jan O. Nehlin, Kilian Vomstein, Lars Blønd, Lisbet Rosenkrantz Hölmich, Kristoffer Weisskirchner Barfodβ-Galactosidase (SA-BGAL) activity assay kit for flow cytometry modified to manufacturer’s protocol (Abcam, Cat# ab228562). The cells were seeded in 24-wells with a seeding density of 1 × 105 cells per well and grown in expansion

(16) TGFβ1 Induces Senescence and Attenuated VEGF Production in Retinal PericytesBiomedicinesJune 14, 2022Dragana Avramovic, Sébastien A. Archaimbault, Alicia M. Kemble, Sabine Gruener, Mirjana Lazendic, Peter D. Westenskow(SRP6552-5UG) was purchased from Sigma (St. Louis, MO, USA). 2.3. Senescence Assay An assay kit from Abcam (ab228562) was used to identify senescent cells. Pericytes were seeded (5 × 105 cells per well) in a 24-well plate

(17) Gingerenone A Induces Antiproliferation and Senescence of Breast Cancer CellsAntioxidants (Basel, Switzerland)March 19, 2022Yu TJ, Tang JY, Shiau JP, Hou MF, Yen CH, Ou-Yang F, Chen CY, Chang HW.and fluorescence microscopy. For flow cytometry, senescent cells were mixed using a Senescence Detection Kit (Fluorometric) (ab228562; Abcam) [26] with the requirements of 37 °C and 60 min, measured by flow cytometer (Guava easyCyte),

(18) Apremilast prevents IL‑17‑induced cellular senescence in ATDC5 chondrocytes mediated by SIRT1International journal of molecular medicineMarch 1, 2021Wang B, Sun W, Bi K, Li Y, Li F.senescence-associate d-β-galactosidase (SA-β-gal) staining by a Cellular Senescence Assay kit (product code ab228562; Abcam) according to the instructions from the manufacturer. Briefly, the cells were quickly washed

(19) G-tract RNA removes Polycomb repressive complex 2 from genesNature structural & molecular biologyOctober 1, 2019Beltran M, Tavares M, Justin N, Khandelwal G, Ambrose J, Foster BM, Worlock KB, Tvardovskiy A, Kunzelmann S, Herrero J, et al.cell senescence The proportion of senescent cells was measured using the Senescence Assay Kit (Abcam ab228562). Cells were treated as described above, stained for 2 hours and washed according to the manufacturer’s

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Beta Galactosidase Assay Kit (Senescence) from Abcam

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Beta Galactosidase Assay Kit (Senescence) from Abcam

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