Hydroxyproline Assay Kit (Colorimetric) ab222941 from Abcam

Product Specs
ItemHydroxyproline Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222941
Sample TypeUrine, Serum, Other biological fluids, Tissue Homogenate
Assay Time3h 0m
Detection Range0.1 - 1 µg
Quantity100 Test

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Caffeic acid phenethyl ester ameliorates high-fat diet-induced muscle textural deterioration in grass carp (Ctenopharyngodon idellus) by modulating adipose-muscle crosstalk via myostatin-taz signalingAnimal NutritionJune 1, 2026Handong Li, Zhiqi Tian, Mingkui Wei, Li Tang, Hong Ji, Gen He, Jian Sunmuscle tissues The hydroxyproline content in fish muscle was quantified using a commercial assay kit (Ab222941, Abcam Trading (Shanghai) Co., Ltd., Shanghai, China). Muscle samples (100 mg) were homogenized in 100

(2) Dual knockdown of Alox15 and TGF-β1 by lipid nanoparticle-delivered siRNA in bleomycin-induced pulmonary fibrosisBiochem Biophys RepMarch 12, 2026Chia-Wei Chang, Jiun-Han Lin, Chen-Chi Liu, Tien-Wei Hsu, Han-Shui Hsu, Shih-Chieh Hungassay Collagen levels in murine lung tissues were measured using the hydroxyproline colorimetric assay kit (ab222941, Abcam, United Kingdom) according to the manufacturer's protocol. Briefly, 10 mg of lung tissue was

(3) Profibrotic predictive toxicology in the lungFrontiers in PharmacologyFebruary 18, 2026Pooja Singh, Rajesh Sinha, Veena B. Antonyhomogenized. Collagen content in mouse lung tissue and MiLO was assessed by the Hydroxyproline assay (ab222941, Abcam) following the manufacturer’s instructions. The soluble collagen levels in supernatants from

(4) Stimulator of Interferon Genes Is a Fine-Tuner, but Not a Prime Mover, of Kidney InflammationThe American Journal of PathologyFebruary 1, 2026Madiha Zahra Syeda, Emily S.H. Yeung, Lisa Y.Q. Hong, Suzanne L. Advani, Youan Liu, Laurette Geldenhuys, Ferhan S. Siddiqi, Veera Ganesh Yerra, Sri Nagarjun Batchu, Andrew AdvaniHALO. Hydroxyproline Content Kidney hydroxyproline was determined using a Hydroxyproline assay kit (catalog number ab222941; lot number 1086984-1; Abcam) and normalized to total protein measured by Quick Start Bradford 1 × Dye

(5) Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagyFuture Science OADecember 31, 2025Yan Zhang, Wen Ma, Fang Wanfor 20 min at 100 °C. The levels of hydroxyproline were measured by the hydroxyproline assay kit (#ab222941, Abcam) in accordance with the operation instruction. 2.11. Pathological examination Lung tissues

(6) Peptidyl-tRNA hydrolase 2 is a negative regulator of peripartum cardiomyopathy with heart failure in female miceNature CommunicationsDecember 19, 2025Vanessa Montoya-Uribe, Pooja Choubey, Chad B. Walton, Natalija Glibetic, Won Seok Yang, Femke J. Aan, Sarah Lindsey, Karolina Peplowska, Brenda Y. Hernandez, Joe W. Ramos, et al.controls were excised and LVs used to perform Hydroxyproline Assays following the manufacturer’s protocol (ab222941, Abcam, Waltham, MA, USA). Briefly, 10 N NAOH was added to each sample and subjected to 1 h of hydrolysis

(7) Aggravated effects of human parvovirus B19 NS1 protein on bleomycin‑induced pulmonary fibrosisMol Med RepDecember 16, 2025Tsai-Ching Hsu, Chih-Chen Tzang, Chia-Wei Kuo, Zhi-Han Wen, Der-Yuan Chen, Bor-Show Tzangprocessed and hydrolyzed according to the manufacturer's instructions using a Hydroxyproline Assay Kit (ab222941, Abcam). After homogenization, hydrolysis, and neutralization, samples were centrifuged at 10,000 ×

(8) Ferret model of bleomycin-induced lung injury shares features of human idiopathic pulmonary fibrosisnpj Regenerative MedicineNovember 21, 2025Shuang Wu, Ian Driver, Meihui Luo, Hikaru Miyazaki, Smitha Shambhu, Dimitry Popov, Liyuan Yang, Jing Wang, Jia Ma, Junfeng Guo, et al.isolation of total protein. The HYP content of each sample was measured using a hydroxyproline assay kit (Ab222941, Abcam, Waltham, MA) according to the manufacturer’s instructions. The HYP content of plasma is presented

(9) Fibroblast Has2 limits acute heart failure following myocardial infarction in male micePhysiological ReportsNovember 18, 2025Danielle T. Little, Kenneth R. Brittian, Caitlin Howard, Emma Pendergraft, Casey Colley, Ning Chen, Yu Yamaguchi, Richa Singhal, Joseph B. Moore, Marcin Wysoczynski, et al.HCl and hydroxyproline concentration in samples was observed using the Hydroxyproline Assay (Abcam Ab222941), as we previously reported (Little et al., 2025). 2.13 Hyaluronan isolation, sizing, and analysis Hyaluronan

(10) Homocysteine Exacerbates Pulmonary Fibrosis via Orchestrating Syntaxin 17 Homocysteinylation of Alveolar Type II CellsAdvanced ScienceSeptember 24, 2025Jiefeng Huang, Ke Fang, Wuyan Lu, Rongrong Wu, Yuanyuan Chen, Linxiao Li, Zihao Hu, Zixin Cai, Yu Jiang, Xinli Fan, et al.Tissues Tissue hydroxyproline content was measured using Hydroxyproline Assay kit (Colorimetric) (ab222941, Abcam). 10 n concentrated NaOH was added to the samples and hydrolyze at 120 °C for 1 h. Then, samples

(11) Exploring multiorgan mitochondrial dysfunction in the switch toward progressive MASLD in AMLN miceiScienceSeptember 19, 2025Meroni M, Paolini E, Longo M, Battistin M, Dondossola D, Ripolone M, Napoli L, Mosca E, Corti S, Dongiovanni P.Aldrich,Cat#C5138 Clarity Western ECL Substrate,Bio-Rad,Cat#1705061 Hydroxyproline Assay Kit (Colorimetric),Abcam,Cat#AB222941 Triglyceride Assay Kit - Quantification,Abcam,Cat#AB65336 Total Cholesterol & Cholesteryl Ester Colorimetric Assay Kit

(12) Mesenchymal stem cell-derived small extracellular vesicles facilitate repair of acute obstruction-induced colonic anastomosis injury by modulating early-stage inflammation in ratsStem cell research & therapyAugust 6, 2025Dong Z, Zhou S, Xia X, Yip HC, Leung KK, Chan MS, Chiu PW.concentration in the colonic anastomosis was measured spectrophotometrically using a hydroxyproline assay kit (ab222941, Abcam, Cambridge, UK) as per manufacturer’s instructions. Measurements were performed at 560 nm. Data

(13) The poly (ADP-ribose) polymerase inhibitor olaparib attenuates established pulmonary fibrosis in a large animal modelBMC Pulm MedAugust 5, 2025Habtamu B. Derseh, Andrew N. Davies, Alarna Young, Sylvie Bischof, Joseph Pelle, David Rudd, Michelle McIntosh, David Piedrafita, Robert J. Bischofbiochemical hydroxyproline assay was used to extrapolate the collagen content (Hydroxyproline Assay kit, ab222941; Abcam), and the assay was performed according to the manufacturer’s instructions. Primary lung fibroblast

(14) Inhibition of BRD4 prevents peribronchial fibrosis in mice with cutaneous lewisite exposureFrontiers in Molecular BiosciencesJuly 30, 2025Huaxiu Zeng, Pooja Singh, Rajesh Sinha, Crystal T. Stephens, Aftab Ahmad, Mohammad Athar, Veena B. Antonyweighed, and homogenized. Collagen content was assessed by Hydroxyproline Assay Kit (Colorimetric) (Abcam, ab222941) following the manufacturer’s instructions. Briefly, samples and standards were incubated with chloramine-T

(15) Collagen hydroxylation couples NAD+/NADH dynamics to tumor dormancy and reactivationResearchSquareJuly 10, 2025Jose Javier Bravo-Cordero, Daniela De Martino, Jaclyn B. Zapateria, Jaclyn Jaclyn Drapela, Stanislav Matteson, Kailie Matteson, Duncan Humphrey, Taylor Humphrey, Betsy Ann Jonhston, Alessandra I. Varghese, et al.assay To assess hydroxyproline levels we used the Hydroxyproline Assay kit (Colorimetric) Abcam (Cat#ab222941). All tissue lysates were prepared from freshly harvested tissues or snap-frozen samples. To homogenize

(16) Regular aerobic exercise ameliorates radiation-induced pulmonary fibrosis: An animal studyClinical and Translational Radiation OncologyJuly 1, 2025Da Bin Ko, Seungwoo Cha, Jae Yong Jeon, Su SSan Kim, Won Kim, Sei Won Lee, Joongkee Min, Si Yeol Song, Seung Hak Leeanother direct indicator of pulmonary fibrosis, was estimated using the hydroxyproline (HYP) assay kit (ab222941, Abcam, USA). In brief, the tissue was homogenized (10 mg per 100 µL D.W), and 100 µL of the sample

(17) MicroRNAs regulate alveolar macrophage homeostasis and its function in lung fibrosisFrontiers in ImmunologyJune 23, 2025Nirmal Parajuli, Yi Yao, Namir Khalasawi, Congcong Yin, Qiong Zhang, Indra Adrianto, Aakash Hans, Li Zhou, Qing-Sheng Mihydroxyproline content was quantified using the colorimetric Hydroxyproline Assay Kit (Abcam, catalog # Ab222941), while H&E- and trichome-stained slides were imaged using the Olympus FSX100 Inverted Microscope (Olympus)

(18) Omaveloxolone, But Not Dimethyl Fumarate, Improves Cardiac Function in Friedreich's Ataxia Mice With Severe CardiomyopathyJournal of the American Heart AssociationJune 17, 2025Lili Salinas, Francisco Figueroa, Claire B. Montgomery, Phung N. Thai, Nipavan Chiamvimonvat, Gino Cortopassi, Elena N. DedkovaT5168 (Sigma Aldrich) as a control. Biochemical Assays Hydroxyproline was evaluated using Abcam kit (ab222941) according to manufacturer's instructions (Abcam, Cambridge, UK). ATP levels were assessed using a fluorometric

(19) Ferret model of bleomycin-induced lung injury shares features of human idiopathic pulmonary fibrosisBIORXIVMay 14, 2025Shuang Wu, Ian Drive, Meihui Luo, Hikaru Miyazaki, Smitha Shambhu, Dimitry Popov, Liyuan Yang, Jing Wang, Jia Ma, Junfeng Guo, et al.isolation of total protein. The HYP content of each sample was measured using a hydroxyproline assay kit (Ab222941, Abcam, Waltham, MA) according to the manufacturer’s instructions. The HYP content of plasma is presented

(20) Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosisNature communicationsMay 10, 2025Seasock MJ, Shafiquzzaman M, Ruiz-Echartea ME, Kanchi RS, Tran BT, Simon LM, Meyer MD, Erice PA, Lotlikar SL, Wenlock SC, et al.(Sigma, CS0030) following manufacturer instructions. Hydroxyproline Colorimetric Assay Kit (Abcam, ab222941) was used to measure collagen in lung lobes following kit instructions and optical density was measured

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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