Hydroxyproline Assay Kit (Colorimetric) ab222941 from Abcam

Product Specs
ItemHydroxyproline Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222941
Quantity100 Test
Sample TypeUrine, Serum, Other biological fluids, Tissue Homogenate
Assay Time3h 0m
Detection Range0.1 - 1 µg

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(1) Ginsenoside Rg2 attenuates TGF-β1/Smad-mediated fibrotic remodeling in aged male mice with sarcopeniaJournal of Ginseng ResearchNovember 1, 2026Yu Liu, Yuxin Zou, Honglin Chen, Qi Shang, Kun Chen, Feng Lin, Peng Zhang, You Zhang, Weicheng Qin, Huiwen Liu, et al.. 2.12 Hyp assay The collagen content in the gastrocnemius (GAS) was quantified using the Hyp assay (Ab222941, Abcam, UK). The procedure was performed according to the manufacturer's instructions, and the absorbance

(2) Extracellular RNA clearance by non-canonical cell-surface MDA5 protects against lung fibrosisEMBO Mol MedOctober 12, 2026Takashi Ishii, Masafumi Horie, Hirokazu Urushiyama, Yusuke Murakami, Tomoya Narita, Ryutaro Fukui, Kensuke Miyake, Akira Saito, Naomi Yamashita, Keiki Kumano, et al.Ultra,Nacalai Tesque,Cat# 11644-40 Hydroxyproline Colorimetric Assay Kit,BioVision (now Abcam),Cat# K555 (ab222941) Mouse IFIH1/MDA5 ELISA Kit,LSBio,Cat# LS-F19794 Mouse TNF-alpha ELISA MAX Standard,BioLegend,Cat#

(3) Matrix Metalloproteinases Inhibitory Effects of Silver Diamine Fluoride: Influence of Time and Potassium Iodide, In VitroInternational Journal of Paediatric DentistrySeptember 18, 2026Lamis El Sharkasi, Lynne Bingle, Helen Rodd, Nicolas Martin, Samiya Subka, Chris DeeryAnalysis of Collagen Integrity Using Hydroxyproline Assay A hydroxyproline colorimetric assay kit (ab222941, Abcam, Cambridge, UK) was used to measure collagen degradation in the incubation media (natural saliva)

(4) Umbilical cord-derived mitochondrial transplantation restores tendon structure and function in a preclinical model of achilles tendinopathyJ Transl MedAugust 7, 2026Da-Yoon Kim, Mi Jin Kim, Min-Jeong Park, Mina Lim, Eun-Seo Back, Chang-Koo Yun, Seong Hun Kim, Hyuk Nam Kwon, Chaeyoung Lee, Kyunghoon Min, et al.distribution. Hydroxyproline content measurement Collagen content was quantified by hydroxyproline assay (Abcam, ab222941). Tendon tissues were snap-frozen, hydrolysed, and processed according to the manufacturer’s protocol.

(5) Endothelial GDF15 deficiency enhances barrier function and mitigates pulmonary fibrosisJCI InsightJuly 8, 2026Kristen Raffensperger, Marta Bueno, Brian J. Philips, Megan Miller, Máté Katona, Shuai Yuan, Adriana Estrada-Bernal, Byron Chuan, Pavan Suresh, Stephanie Taiclet, et al.manufacturer’s protocol. Hydroxyproline was quantified using a colorimetric hydroxyproline assay (Abcam, ab222941). EBD assessment. Animals received either saline or bleomycin (day 0). At day 7, the animals underwent

(6) A decline in skeletal muscle NOX4 abrogates exercise-induced adaptive homeostasis and exacerbates biological agingSci AdvJune 12, 2026Chrysovalantou E. Xirouchaki, Esther García-Domínguez, Eamon Coughlan, Meagan J. McGrath, Saveen Giri, Shuwei Liang, Florian Wiede, Anne Bigot, Junichi Sadoshima, Maria C. Gomez-Cabrera, et al.University of Iowa (Iowa City, IA 52242). The Mouse GDF-15 ELISA Kit (ab216947), Hydroxyproline Assay Kit (ab222941), Mouse ALT ELISA Kit (ab282882), Mouse AST ELISA Kit (ab263882), and OxyBlot Protein Oxidation Detection

(7) Caffeic acid phenethyl ester ameliorates high-fat diet-induced muscle textural deterioration in grass carp (Ctenopharyngodon idellus) by modulating adipose-muscle crosstalk via myostatin-taz signalingAnimal NutritionJune 1, 2026Handong Li, Zhiqi Tian, Mingkui Wei, Li Tang, Hong Ji, Gen He, Jian Sunmuscle tissues The hydroxyproline content in fish muscle was quantified using a commercial assay kit (Ab222941, Abcam Trading (Shanghai) Co., Ltd., Shanghai, China). Muscle samples (100 mg) were homogenized in 100

(8) Regimen‐dependent glucocorticoid effects improve muscle performance without altering CNS physiology in mdx miceJ PhysiolMay 28, 2026Gretel S. Major, Jiayi Chen, Eva van den Berg, Deirdre L. Merry, Angus Lindsayhydroxyproline content of the left hemisphere of the diaphragm according to the manufacturer's instructions (AB222941, Abcam, Cambridge, MA, USA). Statistics All data are reported as mean ± SD unless otherwise stated.

(9) Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in PigsJACC Basic Transl SciApril 29, 2026Noreen Ahmed, Sandrine Parent, Charlotte A. René, Nevena Kovacina, Jennifer St. Amant, Melody Napoleone, Berenice Collet, Saad Kahn, David Courtman, Duncan J. Stewart, et al.Masson's trichrome staining. A subset of the tissue underwent analysis for hydroxyproline content (ab222941, Abcam), an indicator of collagen content and turnover.25,26 All animals completed the study with no

(10) Sushi, von Willebrand Factor Type A, EGF and Pentraxin Domain‐Containing Protein 1: A Novel Fibroblast‐Derived Circulating Biomarker Reflecting Cardiac FibrosisJ Am Heart AssocApril 27, 2026Naoya Kuwahara, Manabu Nagao, Yu Izawa, Kenta Kaneshiro, Hiromi Hashimura, Takeshi Yoshida, Takuo Emoto, Takayoshi Toba, Hidekazu Tanaka, Tatsuro Ishida, et al.neutralized with 10 N HCl. Hydroxyproline levels in the supernatant were measured using a kit (Abcam, #ab222941) following the manufacturer's protocol. Statistical Analysis Normality of continuous variables was

(11) VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblastsExperimental & Molecular MedicineApril 20, 2026Shan Huang, Qianwen Zhao, Tinghui Shao, Chenghao Zhu, Yujia Xue, Naxia Chen, Yue Zhang, Huihui Xu, Ming Kong, Rui Wangobservation. Hydroxyproline quantification Tissue hydroxyproline levels were quantified with a commercially available kit (ab222941, Abcam), as per vendor's recommendation. Briefly, heart tissues (~10 mg) were homogenized with the BeadBlaster

(12) Dual knockdown of Alox15 and TGF-β1 by lipid nanoparticle-delivered siRNA in bleomycin-induced pulmonary fibrosisBiochem Biophys RepMarch 12, 2026Chia-Wei Chang, Jiun-Han Lin, Chen-Chi Liu, Tien-Wei Hsu, Han-Shui Hsu, Shih-Chieh Hungassay Collagen levels in murine lung tissues were measured using the hydroxyproline colorimetric assay kit (ab222941, Abcam, United Kingdom) according to the manufacturer's protocol. Briefly, 10 mg of lung tissue was

(13) Modified fibrin sealant-cellulose efficacy on wound healing: Histopathological studyOpen Vet JFebruary 28, 2026Hussein I. Kareem, Abbas Hadi Jasim Al-Mahmoudi, Muslim F. Diwan, Hala Abbas Naji, Amir I. Towfikestimated using a commercially available colorimetric determination of hydroxyproline assay kit (Cat. No. ab222941, Abcam, Cambridge, UK). Bacterial swab culture Daily, sterile cotton swabs were used to collect the

(14) Profibrotic predictive toxicology in the lungFrontiers in PharmacologyFebruary 18, 2026Pooja Singh, Rajesh Sinha, Veena B. Antonyhomogenized. Collagen content in mouse lung tissue and MiLO was assessed by the Hydroxyproline assay (ab222941, Abcam) following the manufacturer’s instructions. The soluble collagen levels in supernatants from

(15) PLK1 inhibition by volasertib suppresses key transcriptional regulators underlying fibroblast activation and pulmonary fibrosisAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyFebruary 1, 2026Priyanka Singh, Pradeep K. Patel, Rajesh K. Kasam, Ryan Lawson, Harshavardhana H. Ediga, Anil G. Jegga, Satish K. MadalaEstimation Total lung hydroxyproline content was measured using a colorimetric assay (Abcam, Cat. No. ab222941), following the manufacturer’s protocol and as previously described (32). In brief, lung tissue was

(16) Bleomycin-induced lung fibrosis and dysfunction is exacerbated by G6PD deficiencyAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyFebruary 1, 2026Christina Signoretti, Samuel Fatehi, Rhonda Drewes, Francesca Cendali, Monika Dzieciatkowska, D’Alessandro D’Alessandro, Yongho Bae, Sachin A. Guptetogether in Image J. Hydroxyproline assay: The Hydroxyproline assay kit was purchased from Abcam (Cat#ab222941), and the assay was performed as per the manufacturer’s protocol. Briefly, 10 mg/100 μL lung tissue

(17) Stimulator of Interferon Genes Is a Fine-Tuner, but Not a Prime Mover, of Kidney InflammationThe American Journal of PathologyFebruary 1, 2026Madiha Zahra Syeda, Emily S.H. Yeung, Lisa Y.Q. Hong, Suzanne L. Advani, Youan Liu, Laurette Geldenhuys, Ferhan S. Siddiqi, Veera Ganesh Yerra, Sri Nagarjun Batchu, Andrew AdvaniHALO. Hydroxyproline Content Kidney hydroxyproline was determined using a Hydroxyproline assay kit (catalog number ab222941; lot number 1086984-1; Abcam) and normalized to total protein measured by Quick Start Bradford 1 × Dye

(18) Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagyFuture Science OADecember 31, 2025Yan Zhang, Wen Ma, Fang Wanfor 20 min at 100 °C. The levels of hydroxyproline were measured by the hydroxyproline assay kit (#ab222941, Abcam) in accordance with the operation instruction. 2.11. Pathological examination Lung tissues

(19) Peptidyl-tRNA hydrolase 2 is a negative regulator of peripartum cardiomyopathy with heart failure in female miceNature CommunicationsDecember 19, 2025Vanessa Montoya-Uribe, Pooja Choubey, Chad B. Walton, Natalija Glibetic, Won Seok Yang, Femke J. Aan, Sarah Lindsey, Karolina Peplowska, Brenda Y. Hernandez, Joe W. Ramos, et al.controls were excised and LVs used to perform Hydroxyproline Assays following the manufacturer’s protocol (ab222941, Abcam, Waltham, MA, USA). Briefly, 10 N NAOH was added to each sample and subjected to 1 h of hydrolysis

(20) Aggravated effects of human parvovirus B19 NS1 protein on bleomycin‑induced pulmonary fibrosisMol Med RepDecember 16, 2025Tsai-Ching Hsu, Chih-Chen Tzang, Chia-Wei Kuo, Zhi-Han Wen, Der-Yuan Chen, Bor-Show Tzangprocessed and hydrolyzed according to the manufacturer's instructions using a Hydroxyproline Assay Kit (ab222941, Abcam). After homogenization, hydrolysis, and neutralization, samples were centrifuged at 10,000 ×

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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