Lipid Peroxidation (4-HNE) Assay Kit ab238538 from Abcam

Product Specs
ItemLipid Peroxidation (4-HNE) Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab238538
Quantity32 Test, 96 Test
Sample TypeSerum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
Research AreaImmuno-oncology

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Website: http://www.abcam.com

(1) Unusual Switch of Cell Death Mode by Docosahexaenoic Acid (DHA) via Enhancing Endoplasmic Reticulum Peroxidation Induced by Lipophilic Iron ComplexAntioxidants (Basel)October 9, 2026Bo Shao, Zhi-Guo Sheng, Huan Li, Shun-Li Jiang, Gui-Zhi Han, Chen-Jun Shao, Ben-Zhan Zhufollowing standard procedures using commercially available diagnostic kits (4-HNE assay kit [Abcam, ab238538]). 2.6. Electron Spin Resonance (ESR) Spin-Trapping Studies We used Fe(III), AA, and H2O2, together

(2) Mitochondrial membrane lipid cardiolipin controls fiber-type adaptations in aging muscle via estrogen-related receptor γNature AgingSeptember 29, 2026Fabian Finger, Mikkel Frost, Shinya Watanabe, Tao Ma, Taewook Kang, Dipsikha Biswas, Ruben Zapata-Perez, Vincent Anton, Lucile Dollet, Lasse K. Markussen, et al.level of 4-HNE in muscle tissue lysates was measured with the 4-HNE Assay Kit (Abcam, catalog number ab238538), according to the manufacturer’s instructions. Statistics and reproducibility Unless otherwise stated,

(3) Lean adipocyte oxylipin signaling restrains breast cancer through ferroptosisScienceSeptember 10, 2026Meghan C. Curtin, Abigail E. Jackson, Mark D. Lee, Elisabeth A. Brown, J. Alan Maschek, David H. Lum, James E. Cox, Alana L. Welm, Keren I. HilgendorfELISA ELISA for 4-HNE was performed according to Abcam Lipid peroxidation Assay Kit instructions (ab238538). Briefly, wells were coated with 100 μL of 4-HNE conjugate and incubated overnight at 4 °C. The next

(4) ACT001 suppresses ox-LDL-mediated macrophage ferroptosis via activating the NRF2 pathway: An in vitro mechanistic study relevant to atherosclerosisExp Ther MedSeptember 7, 2026Yangyang Tao, Yuwu Chen, Yonghou Zhao, Jianbo Caigraphs. Measurement of 4-HNE Measurement of 4-HNE levels was performed using the 4-HNE assay kit (cat. no. ab238538, Abcam). In total, 50 µl standards or samples were added to each well of the 4-HNE conjugate-coated

(5) Apabetalone Drives a Metabolic Shift Towards Ketogenesis and Reduces Liver Steatosis in Diet-Induced Obesity MiceBiomedicinesJuly 22, 2026Laura M. Tsujikawa, Agostina Carestia, Sylwia Wasiak, Christopher D. Sarsons, Ravi Jahagirdar, Salman Azhar, Dean Gilham, Derek Li, Li Fu, Jan O. Johansson, et al.and stored at −80 °C until use. Lipid peroxidation was measured using the 4-HNE Assay Kit from Abcam (ab238538) following the kit protocol. For this assay, three hearts were pooled per replicate with three groups

(6) Danshen (Salvia miltiorrhiza Buge)-Gegen (Pueraria lobata (Willd.) Ohwi) Herb Pair Inhibits Ferroptosis After Ischemia-Reperfusion Injury Involving the Nrf2/System xc-/GPX4 AxisAntioxidants (Basel)July 17, 2026Yin Liu, Yan Wang, Xinyu Shi, Ruomei Che, Xiaoli HePeroxidation Activity Assay Kit (ab273334, Abcam, Cambridge, MA, USA) [33]. The intracellular 4-HNE (ab238538, Abcam, Cambridge, MA, USA) concentration was determined using the 4-HNE Assay Kit [39]. All samples,

(7) Melatonin reprograms antioxidant defenses to suppress ferroptosis via Homer1a/mGluR1-Nrf2/xCT signaling after retinal ischemia-reperfusionRedox BiolJuly 10, 2026Ya-Nan Dou, Yuwen Wen, Yuan Huang, Xiuquan Wu, Zehan Zhang, Guanyi Wang, Wangshu Chao, Dongyu Wei, Weihao Lv, Xiaowei Fei, et al.group using a malondialdehyde (MDA) lipid peroxidation assay kit (S0131 M, Beyotime®), 4-HNE assay kit (ab238538, Abcam), GSSG/GSH quantification kit II (G263,Dojindo®), SOD assay kit (S311, Dojindo®), ROS assay kit-highly

(8) Dietary Tryptophan Supplementation Attenuates Lipopolysaccharide-Induced Acute Lung Injury in a Murine Model of ColitisNutrientsJune 23, 2026Hsiao-Ching Lai, Hitoshi Shirakawa, Afifah Zahra Agista, Yi-Ping Hao, Suh-Ching Yang, Ming-Tsan Lin, Sung-Ling Yeh, Chiu-Li YehArbor, MI, USA) as previously described [24]. 4-HNE levels were measured using an Assay Kit (cat. no. ab238538, Abcam, Cambridge, London, UK). TBARS and 4-HNE levels were expressed based on the amount of protein

(9) A Cu-based metal-organic framework based on desloratadine and siHIF-1α for achievement of genes/drugs/CDT combination therapy in hepatocellular carcinomaJournal of NanobiotechnologyApril 20, 2026Ding Ding, Zhe Huang, Lu Wang, Ziwen Wang, Juan Qin, Man Ni, Baiyu Yao, Yanchao Yangby incubation with HIF-1α (1:100; AF1009, Affinity), Ki67 (1:100; AF0198, Affinity), 4-HNE (1:100; ab238538, Abcam), 8-OHdG (1:100; ab285254, Abcam), p-PERK (1:200; DF7576, Affinity), p-IRE1α (1:100; AF7150,

(10) SGLT2 Inhibitor Dapagliflozin Attenuates Cardiomyocyte Injury and Inflammation Induced by PI3Kα-Selective Inhibitor Alpelisib and Fulvestrant Under HyperglycemiaInt J Mol SciApril 17, 2026Vincenzo Quagliariello, Massimiliano Berretta, Matteo Barbato, Fabrizio Maurea, Maria Laura Canale, Andrea Paccone, Irma Bisceglia, Andrea Tedeschi, Marino Scherillo, Jacopo Santagata, et al.measured using a human-specific ELISA-based lipid peroxidation assay (Lipid Peroxidation 4-HNE Assay Kit, ab238538; Abcam, Milan, Italy). Standards and samples were added to pre-coated 96-well plates and incubated according

(11) The HIF-2 transcription factor mediates resistance to ferroptosis in pancreatic cancerMol CellApril 2, 2026Maimon E. Hubbi, Catherine L. Wang, Yasir Suhail, Nadia L. Almasri, Jane Xie, Erin E. Hollander, Kshitiz, Alexander Muir, Ben Z. Stanger, Chi V. Dangcommercial assays Cell-Titer Glo,Promega,Cat# 7570 Lipid Peroxidation (4-HNE) Assay Kit,Abcam,Cat# ab238538 Dual Luciferase Assay Kit,Promega,Cat# E1910 Chromatin IP Kit,Cell Signaling Tech,Cat# 9003 RNeasy

(12) Therapeutic potential of dihydronicotinamide riboside (NRH) on obesity and glucose intolerance in miceNature CommunicationsMarch 25, 2026Marie Rumpler, Guido van Mierlo, Kasper T. Vinten, Maria Pilar Giner, Stefan Christen, Faisal Hayat, Mikhail V. Makarov, Vincent Gardeux, Julie Russeil, Bauke V. Schomakers, et al.measurements The detection of 4-HNE in mASCs, liver and eWAT was performed with a commercial kit (ab238538, ABCAM) using 100 mg of protein for cellular lysates and 200 mg for eWAT and liver lysates. Statistical

(13) Ferroptosis‐Driven Neuronal Damage Exacerbation in Diabetic Stroke: Implications of SLC7A11 InhibitionCNS Neuroscience & TherapeuticsMarch 12, 2026Siyuan Liu, Min Liu, Binbin Wang, Miao Sun, Huikai Yang, Mengyao Qu, Libin Ma, Likai Shi, Yuxiang Song, Peng Li, et al.and 4‐HNE were measured using the MDA Assay Kit (Nanjing Jiancheng, China) and the 4‐HNE Assay Kit (ab238538, Abcam, UK), in accordance with the manufacturer's protocols. Results for MDA and 4‐HNE were expressed

(14) Visnagin Protects Against Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting Oxidative Stress and Reducing FerroptosisInt J Med SciFebruary 18, 2026Sheng-Wen Wu, Chen-Yu Chiang, Chien-Ying Lee, Shiuan-Shinn Lee, Wen-Ying Chen, Chun-Jung Chen, Ching-Chi Tseng, Yin-Che Lu, Yu-Hsiang Kuan(4-HNE) by using the TBARS assay kit (Cat: 10009055, Cayman, MI, USA) and 4-HNE ELISA assay kit (Cat: ab238538, Abcam, MA, USA), respectively. Fe²⁺ concentrations were determined using an iron assay kit (Cat: ab83366,

(15) CREB5 Inhibits Neuronal Ferroptosis via Transactivating ApoL6 to Regulate Lipid Droplet Metabolism After Spinal Cord InjuryCNS Neuroscience & TherapeuticsFebruary 17, 2026Xiaolong Xi, Zhensen Chen, Chaojun Wang, Fei Wang, Xuedong Sunacquisition of precise quantitative data on ROS. Additionally, a specialized detection kit (ab118970, ab238538, Abcam) was used to measure the levels of lipid peroxidation products in lysates of primary neurons,

(16) Mitochondrial energetic failure underlies FLVCR1-related sensory neuropathyCommunications BiologyFebruary 14, 2026Francesca Bertino, Diletta Isabella Zanin Venturini, Eleonora Grasso, Joanna Kopecka, Chiara Salio, Barbara Gnutti, Ram Manohar Basnet, Stefania Bellini, Luca Mignani, Boxun Zhao, et al.cytosolic extracts, obtained as reported above, by using the Lipid Peroxidation (4-HNE) Assay Kit (Abcam; ab238538) that evaluates the 4-hydroxy-nonenal, one of the thiobarbituric reactive substances used as an indicator

(17) The effects of a (poly)phenol‐rich food intervention on markers of exercise‐induced inflammation and oxidative stress: A randomised controlled trialExp PhysiolJanuary 23, 2026Abrar Al Hebshi, Josh Thorley, Ana Rodriguez‐Mateos, Zicheng Zhang, Lewis J James, Tom Cliffordwas quantified as a biomarker of lipid peroxidation with a commercially available assay kit (cat. no. ab238538, Abcam, Cambridge, UK). 8‐Hydroxy‐2′‐deoxyguanosine (8‐OHdG) was quantified in urine as a biomarker

(18) Modified Sanjia Powder ameliorates cognitive impairment and exerts neuroprotective effects in 5 × FAD mice: insights from quantitative proteomicsFrontiers in NutritionJanuary 16, 2026Yifan Wang, Chenxi Liu, Xiaoyan Zheng, Zhiqiang Wang, Yufan Wang, Yi Geng, Jin Yang, Kaifeng Wei, Xiaoying ChenPeroxidation (MDA) Assay Kit (Sigma-Aldrich, MAK085) and Lipid Peroxidation (4-HNE) Assay Kit (Abcam, ab238538), following the manufacturer's instructions. 2.11 Statistical analysis Statistical analyses were performed

(19) Osteoarthritis pathogenesis Unveiled: DMT1 drives autophagy-dependent ferroptosis through a mir-17-5p/NEDD4 regulatory axisJournal of Orthopaedic TranslationJanuary 8, 2026Guanglei Zhao, Yue Shen, Jingchun Ma, Xin Huang, Jie Chen, Jingsheng Shi, Jun xia, Siqun Wang, Gangyong Huang, Weigang Wu4-HNE The MDA Assay kit (E-BC-K025-M, Elabscience), GSH Assay Kit (KTB1600, Abbkine) and 4-HNE Assay Kit (ab238538, Abcam), were employed for determination in accordance with the manufacturer's guidelines. The intracellular

(20) Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathwayCancer Cell InternationalJanuary 6, 2026Chenchen Yu, Tingting Zhang, Jialu Yuan, Yijing Su, Hongli Zhang, Liqin Xu, Xiaomin Li, Jianan Cui, Rui Xu, Yan Zhou, et al.Cystine uptake fluorometric assay kit (E-BC-F066) were purchased from Elabscience (Wuhan, China). Lipid peroxidation (4-HNE) assay (ab238538) and Glutamate assay (ab83389) were purchased from Abcam (Shanghai, China). The primary antibodies,

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Lipid Peroxidation (4-HNE) Assay Kit from Abcam

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Lipid Peroxidation (4-HNE) Assay Kit from Abcam

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