DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
TypeStain
Sample TypeAdherent cells, Suspension cells
Quantity2 mL

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(161) Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 RevStructureOctober 1, 2023Elif Eren, Norman R. Watts, Davide Randazzo, Ira Palmer, Dan L. Sackett, Paul T. Wingfield11873580001 Nocodazole,Sigma-Aldrich,Cat# M1404-2MG Taxol (paclitaxel),Invitrogen,Cat# P3456 DAPI staining solution,Abcam,Cat# ab228549 Lipofectamine 3000,Invitrogen,Cat# L3000015 Opti-MEM,Gibco,Cat# 31985070 Subtilisin,Hampton Research

(162) Loss of Grin2a causes a transient delay in the electrophysiological maturation of hippocampal parvalbumin interneuronsCommunications BiologySeptember 19, 2023Chad R. Camp, Anna Vlachos, Chiara Klöckner, Ilona Krey, Tue G. Banke, Nima Shariatzadeh, Sarah M. Ruggiero, Peter Galer, Kristen L. Park, Adam Caccavano, et al.temperature. Slices were washed 3 times in PBS for 10 min then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 min before being washed 3 more times in PBS for 10 min. Slices were mounted on Superfrost

(163) Microglial SIRT1 activation attenuates synapse loss in retinal inner plexiform layer via mTORC1 inhibitionJournal of neuroinflammationSeptember 5, 2023Yao K, Mou Q, Lou X, Ye M, Zhao B, Hu Y, Luo J, Zhang H, Li X, Zhao Y.anti-Iba1 antibody (ab5076 and ab178846; Abcam; 1:200); anti-CD68 antibody (ab955; Abcam; 1:50); DAPI (ab228549; Abcam; 1:1000); anti-TSC2 antibody (#4308; cell signaling technology; 1:800); anti-HA-Tag antibody

(164) Curcumin-activated Olfactory Ensheathing Cells Improve Functional Recovery After Spinal Cord Injury by Modulating Microglia Polarization Through APOE/TREM2/NF-κB Signaling PathwayJournal of Neuroimmune PharmacologySeptember 2, 2023Chao Jiang, Zhe Chen, Xiaohui Wang, Yongyuan Zhang, Xinyu Guo, Hong Fan, Dageng Huang, Yuqing He, Xiangwen Tang, Yixiang Ai, et al.1:200, Abcam), pNF-κB (3033, 1:200, Cell Signaling Technology), APOE (ab183597, 1:200, Abcam) and DAPI (ab228549, 1:1000, Abcam). After incubation with the corresponding secondary antibodies, coverslips were washed

(165) Self-oxygenation of engineered living tissues orchestrates osteogenic commitment of mesenchymal stem cellsBiomaterialsSeptember 1, 2023Shabir Hassan, Ting Wang, Kun Shi, Yike Huang, Maria Elizabeth Urbina Lopez, Kaifeng Gan, Mo Chen, Niels Willemen, Haroon Kalam, Eder Luna-Ceron, et al.(Catalog No.: ab113851), primary antibodies for F-actin (Catalog No.: ab205) and DAPI (Catalog No.: ab228549) were purchased from Abcam and used as per the manufacturer’s protocol. Recombinant human fibroblast

(166) Long-term survival of LGR5 expressing supporting cells after severe ototoxic trauma in the adult mouse cochleaFrontiers in Cellular NeuroscienceAugust 24, 2023Natalia Smith-Cortinez, Ferry G. J. Hendriksen, Dyan Ramekers, Robert J. Stokroos, Huib Versnel, Louise V. StraatmanInvitrogen, A-21207), donkey-anti Goat-Alexa 488 (1/500, Abcam, AB150129), and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Lastly, specimens were washed in PBS and mounted in Vectashield Antifade

(167) Identification of a novel enhancer essential for Satb1 expression in T(H)2 cells and activated ILC2sLife science allianceAugust 1, 2023Nomura A, Kobayashi T, Seo W, Ohno-Oishi M, Kakugawa K, Muroi S, Yoshida H, Endo TA, Moro K, Taniuchi I.kept at −20°C until analysis. On the day of the analysis, thymocytes were stained with 1/100 of DAPI (ab228549; Abcam) at room temperature, for 30 min, and washed three times in PBS. Cover slips were mounted on

(168) Integrated single‐cell and spatial transcriptomic profiling reveals higher intratumour heterogeneity and epithelial–fibroblast interactions in recurrent bladder cancerClinical and Translational MedicineJuly 24, 2023Zhen‐Duo Shi, Zhuo Sun, Zuo‐Bin Zhu, Xing Liu, Jun‐Zhi Chen, Lin Hao, Jie‐Fei Zhu, Kun Pang, Di Wu, Yang Dong, et al.ab75813), CCL22 anti‐human Ab (Abcam, ab23772) and 4′,6‐diamidino‐2‐phenylindole staining solution (Abcam, ab228549). Following the manufacturer's instructions (Akoya, Opal Polaris 7 Color Automation IHC Detection Kit),

(169) Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated StrainVirusesJuly 21, 2023Sok Song, Gyu-Nam Park, Jihye Shin, Ki-Sun Kim, Byung-Hyun An, SeEun Choe, Song-Yi Kim, Bang-Hun Hyun, Dong-Jun AnAfter washing, cell nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam; catalog no. ab228549) at room temperature, followed by observation under a fluorescence microscope (200×). 2.6. Virus Growth

(170) Single‐cell landscape analysis reveals systematic senescence in mammalian Down syndromeClinical and Translational MedicineJuly 17, 2023Yao Chen, Yanyu Xiao, Yanye Zhang, Renying Wang, Feixia Wang, Huajing Gao, Yifeng Liu, Runju Zhang, Huiyu Sun, Ziming Zhou, et al.times with 1% BSA in PBS, the slides were incubated with secondary antibodies (Thermo Fisher) and DAPI (ab228549; Abcam) for 1 h at room temperature. TUNEL staining was performed using a one‐step TUNEL Apoptosis Detection

(171) Early maturation and hyperexcitability is a shared phenotype of cortical neurons derived from different ASD-associated mutationsTranslational PsychiatryJuly 6, 2023Yara Hussein, Utkarsh Tripathi, Ashwani Choudhary, Ritu Nayak, David Peles, Idan Rosh, Tatiana Rabinski, Jose Djamus, Gad David Vatine, Ronen Spiegel, et al.solution overnight at 4 °C. On the next day, they were washed in DPBS and incubated with DAPI (Abcam, ab228549, 1:1000) and corresponding secondary antibodies (Abcam, ab150084, ab150117, ab175711, 1:250) for 60

(172) Immunohistologic Features of Cerebral Venous Thrombosis Due to Vaccine-Induced Immune Thrombotic ThrombocytopeniaNeurology(R) neuroimmunology & neuroinflammationJuly 1, 2023Geeraerts T, Guilbeau-Frugier C, Garcia C, Memier V, Raposo N, Bonneville F, Gales C, Darcourt J, Voisin S, Ribes A, et al.temperature. In some instances, wheat germ agglutinin was used. Sections were then stained with DAPI (Abcam, ab228549) for 15 minutes. Sections were labeled with secondary antibodies (Oregon-Green-488 goat antirabbit IgG,

(173) Restoration of normal blood flow in atherosclerotic arteries promotes plaque stabilizationiScienceJune 16, 2023Schake MA, McCue IS, Curtis ET, Ripperda TJ Jr, Harvey S, Hackfort BT, Fitzwater A, Chatzizisis YS, Kievit FM, Pedrigi RM.mg),Apotex Corp.,Cat# 60505-2579-09 Prolong Gold antifade reagent,Invitrogen,Cat#P36930 DAPI,Abcam,Cat#ab228549 Hydrochloric Acid,Sigma-Aldrich,Cat#32031-500ML Ethanol,Decon Laboratories Inc.,Cat#2701 Acetone,Fisher

(174) Chitosomes Loaded with Docetaxel as a Promising Drug Delivery System to Laryngeal Cancer Cells: An In Vitro Cytotoxic StudyInternational Journal of Molecular SciencesJune 8, 2023Christian R. Moya-Garcia, Nicole Y. K. Li-Jessen, Maryam Tabrizian(cat. # ab65393), ALEXA647/EGFR (cat. # ab192982), ALEXA488/TUBIII (cat. # ab195879), and DAPI (cat. # ab228549) from Abcam (Cambridge, UK) were used to visualize the cell membrane, cytoskeleton, and nucleus, respectively.

(175) Enhancement of Collagen-I Levels in Human Gingival Fibroblasts by Small Molecule Activation of HIF-1αJournal of Agricultural and Food ChemistryMay 24, 2023Lucia Adriana Lifshits, Miryam Rabin, Ran Tohar, Francesca Netti, Matan Gabay, Marina Sova, Daniel Z. Bar, Evgeny Weinberg, Lihi Adler-Abramovich, Maayan Gal(ab150077, Abcam)), Goat Anti-Mouse IgG H&L (Alexa Fluor 647) (ab150115, Abcam), and DAPI Staining Solution (ab228549, Abcam). Cellular viability was assessed using a live/dead viability assay (04511, Merck) and CellTiter

(176) Zika virus triggers autophagy to exploit host lipid metabolism and drive viral replicationCell Communication and SignalingMay 19, 2023Gloria Stoyanova, Sidra Jabeen, Joselyn Landazuri Vinueza, Sounak Ghosh Roy, Richard A. Lockshin, Zahra Zakeriwashed with 1X PBS for 5 min and then stained with 4, 6 -diamidino-2-phenylindole (DAPI) (1 mM) (# ab228549, ABCAM) for 8 min. Cells were washed twice with 1 × PBS, mounted, and embedded in Fluoromount® and observed

(177) High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seqNature CommunicationsMay 12, 2023Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, et al.aliquot of nuclei was stained with DAPI (4′,6-diamidino-2-phenylindole) staining solution (Abcam, Cat # ab228549), loaded on a hemocytometer and observed under an inverted fluorescence microscope (Nikon Eclipse, Ts2-FL).

(178) Advanced cell-based products generated via automated and manual manufacturing platforms under the quality by design principle: Are they equivalent or different?HeliyonMay 3, 2023Duc M. Hoang, Quyen T. Nguyen, Trang T.K. Phan, Anh T.L. Ngo, Phuong T. Pham, Trung Q. Bach, Phuong T.T. Le, Hoa T.P. Bui, Liem Nguyen Thanhwere fixed with 4% paraformaldehyde (PFA) and stained with DAPI staining solution (1:5000, Abcam, #ab228549) after the assay. The ImageXpress® Micro Confocal High-content Imaging System (Molecular Devices) was

(179) The mitochondrial Ca 2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationbioRxivMay 3, 2023Emily Fernández García, Usha Paudel, Michael C. Noji, Caitlyn E. Bowman, Jason R. Pitarresi, Anil K. Rustgi, Kathryn E. Wellen, Zolt Arany, Jillian S. Weissenrieder, J. Kevin FoskettScientific, R37174), according to manufacturer’s instructions. After 15-min incubation, 4 mM DAPI (Abcam, ab228549) was added to the suspension and cells were analyzed by FACS with emission filters 530 and 440 nm for

(180) Selective inhibition of somatostatin-positive dentate hilar interneurons induces age-related cellular changes and cognitive dysfunctionPNAS NexusMay 1, 2023Lyu J, Nagarajan R, Kambali M, Wang M, Rudolph U.microscope. To verify viral placements and immunofluorescence labeling of mCherry, DAPI stain (Abcam, cat # ab228549) was added to free-floating 30-µm coronal sections using the concentration of 1 µm in the dark. The

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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