DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(121) Choroid plexus CCL2‒CCR2 signaling orchestrates macrophage recruitment and cerebrospinal fluid hypersecretion in hydrocephalusActa Pharmaceutica Sinica BOctober 1, 2024Qiguang Wang, Fei Liu, Yue Li, Huan Zhang, Xin Qi, Ke Wu, Yi Zhang, Shenglan You, Wenke Liu, Xuhui Hui, et al.anti-rabbit Alexa 550-conjugated IgG (Jackson, 111-025-003). Then the sections were stained with DAPI (Abcam, ab228549) after washing 3 times in PBS at RT. At last, sections were mounted with an anti-fade fluorescence mounting

(122) The destruction of cytoplasmic skeleton leads to the change of nuclear structure and the looseness of lamin A submicroscopic networkHeliyonSeptember 30, 2024Zhenyu Yang, Xianglong Liu, Xiaoliang Li, Maurizio Abbate, Han Rui, Miao Guan, Zhenglong Sunwere washed three times with PBS for 20 min. The labeled cells were stained with DAPI (Abcam, Cat# ab228549) for 15 min, washed three times with PBS for 5 min and stored at 4 °C or imaged immediately. 4.3 Microscopy

(123) Estrogen-dependent gene regulation: Molecular basis of TIMP-1 as a sex-specific biomarker for acute lung injuryPhysiological reportsSeptember 1, 2024Almuntashiri S, Dutta S, Zhu Y, Gamare S, Ramírez G, Irineo-Moreno V, Camarena A, Regino N, Campero P, Hernández-Cardenas CM, et al.primary antibodies. Cells or lung sections were counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI) (ab228549, Abcam, Cambridge, MA). All digital images were visualized at room temperature using a Zeiss Observer

(124) FAT10 inhibits TRIM21 to down-regulate antiviral type-I interferon secretionLife Science AllianceSeptember 1, 2024Kritika Saxena, Katharina Inholz, Michael Basler, Annette AichemX-100/PBS. After two washing steps, A549 cells stably expressing GFP and/or mCherry were stained with DAPI (ab228549, 1:1,000; Abcam) and directly mounted on glass slides using Fluoromount-G mounting media (0100-01; Southern biotech

(125) Genome-wide CRISPR-Cas9 knockout screens identify DNMT1 as a druggable dependency in sonic hedgehog medulloblastomaActa Neuropathologica CommunicationsAugust 7, 2024Foteini Tsiami, Chiara Lago, Noemi Pozza, Federica Piccioni, Xuesong Zhao, Fabienne Lülsberg, David E. Root, Luca Tiberi, Marcel Kool, Jens Schittenhelm, et al.Thermofisher Scientific, A11035) for 1h at room temperature. Nuclei were counterstained with DAPI 10 mM (Abcam, ab228549). Sections and coverslips (Thermofisher Scientific, 15,747,592) were mounted with permanent mounting

(126) Exclusion of sulfide:quinone oxidoreductase from mitochondria causes Leigh-like disease in mice by impairing sulfide metabolismJournal of Clinical InvestigationAugust 1, 2024Eiki Kanemaru, Kakeru Shimoda, Eizo Marutani, Masanobu Morita, Maria Miranda, Yusuke Miyazaki, Claire Sinow, Rohit Sharma, Fangcong Dong, Donald B. Bloch, et al.diluted 1:500 in double-salt PBS containing Triton X-100 (0.3%). The sections were stained with DAPI (ab228549, Abcam) before mounting with Fluoromount-G medium (0100-01, Southern Biotech). The stained sections

(127) Antibody–Drug Conjugate Sacituzumab Govitecan Enables a Sequential TOP1/PARP Inhibitor Therapy Strategy in Patients with Breast CancerClinical Cancer ResearchJuly 15, 2024Aditya Bardia, Sheng Sun, Nayana Thimmiah, James T. Coates, Bogang Wu, Rachel O. Abelman, Laura Spring, Beverly Moy, Phoebe Ryan, Mark N. Melkonyan, et al.for 1 hour. The cells were then washed twice with 2% BSA in PBS and counterstained with DAPI (Abcam, #ab228549). For flow cytometry detection of apoptotic cells, 1 × 106 cells were collected, and the staining was

(128) Exploring the Link Between Autophagy‐Lysosomal Dysfunction and Early Heterotopic Ossification in TendonsAdvanced ScienceJuly 1, 2024Chang‐He Gao, Qian‐Qian Wan, Jan‐Fei Yan, Yi‐Na Zhu, Lei Tian, Jian‐Hua Wei, Bin Feng, Li‐Na Niu, Kai Jiaodilution for 1 h at 37 °C. The cell nuclei were stained using 4′,6‐diamidino‐2‐phenylindole (DAPI; ab228549; Abcam) at room temperature for 15 minutes. For Alizarin Red S staining, the frozen sections were stained

(129) Pulsatile flow increases METTL14-induced m 6 A modification and attenuates septic cardiomyopathy: an experimental studyInternational journal of surgery (London, England)July 1, 2024Zhu S, Wang K, Yu Z, Tang W, Zhang Y, Shinge SA, Qiang Y, Liu H, Zeng J, Qiao K, et al.(ab6721; Abcam, Cambridge, MA, USA) was then added, followed by 4′,6-diamidino2-phenylindole (DAPI) (ab228549; Abcam, Cambridge, MA, USA) staining. For cellular immunofluorescence, CMECs were fixed with 4% paraformaldehyde

(130) Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flapsJournal of NanobiotechnologyJune 14, 2024Gaoxiang Yu, Jian Ding, Ningning Yang, Lu Ge, Nuo Chen, Xuzi Zhang, Qiuchen Wang, Xian Liu, Xuanlong Zhang, Xiaoqiong Jiang, et al.phalloidin-FITC (Actin-Tracker Green; Beyotime, C1033), and the nucleus was stained with DAPI (Abcam, ab228549). The Calcein/PI cell viability and cytotoxicity detection kit (C2015S) were employed, following the

(131) Exclusion of sulfide:quinone oxidoreductase from mitochondria causes Leigh-like disease in mice by impairing sulfide metabolismJ Clin InvestJune 13, 2024Eiki Kanemaru, Kakeru Shimoda, Eizo Marutani, Masanobu Morita, Maria Miranda, Yusuke Miyazaki, Claire Sinow, Rohit Sharma, Fangcong Dong, Donald B. Bloch, et al.diluted 1:500 in double-salt PBS containing Triton X-100 (0.3%). The sections were stained with DAPI (ab228549, Abcam) before mounting with Fluoromount-G medium (0100-01, Southern Biotech). The stained sections

(132) MiR-184 Mediated the Expression of ZNF865 in Exosome to Promote Procession in the PD ModelMolecular neurobiologyJune 1, 2024Liu C, Wang Y, Li JW, Zhu X, Jiang HS, Zhao H, Zhang LM.with a commercial TUNEL assay kit (Abcam; ab206386). Nuclei are stained with DAPI solution (Abcam; ab228549). There are three different fields for every section and four sections per group to analyze. Immunohistochemistry (IHC

(133) Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1Regenerative therapyJune 1, 2024Chen YT, Young TH, Wang YH, Huang CH, Gao YY, Huang TW.Plasma Membrane Stains (C10046, Thermo Fisher Scientific) and 4′,6-diamidino-2-phenylindole (DAPI) (ab228549, Abcam, Cambridge, UK) to demonstrate the cell morphology after 28 days of culture. For better visualization

(134) Antibody–Drug Conjugate Sacituzumab Govitecan Enables a Sequential TOP1/PARP Inhibitor Therapy Strategy in Patients with Breast CancerClin Cancer ResMay 6, 2024Aditya Bardia, Sheng Sun, Nayana Thimmiah, James T. Coates, Bogang Wu, Rachel O. Abelman, Laura Spring, Beverly Moy, Phoebe Ryan, Mark N. Melkonyan, et al.for 1 hour. The cells were then washed twice with 2% BSA in PBS and counterstained with DAPI (Abcam, #ab228549). For flow cytometry detection of apoptotic cells, 1 × 106 cells were collected, and the staining was

(135) Acupuncture Ameliorates Depression-Like Behaviors Through Modulating the Neuroinflammation Mediated by TLR4 Signaling Pathway in Rats Exposed to Chronic Restraint StressMolecular NeurobiologyMay 1, 2024Huili Jiang, Xianqi Long, Yu Wang, Xuhui Zhang, Lu Chen, Xinjing Yang, Bingcong Zhao, Ye Zhang, Yemao Chai, Tuya Baowashed five times with PBS. And the nucleus was restained with DAPI staining solution (1:1000, Abcam, ab228549, USA) and then incubated at room temperature for 5 min. The slides were washed with PBS for 3 times,

(136) Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healingNature communicationsApril 23, 2024Han X, Saengow C, Ju L, Ren W, Ewoldt RH, Irudayaraj J.Secondary Antibody (1:50). ROS/Superoxide detection assay kit (ab139476), calcein AM (ab141420), DAPI (ab228549) were purchased from Abcam. Human mesenchymal stem cell marker antibody panel was purchased from R&D systems

(137) Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assemblyJournal of Cell BiologyApril 1, 2024S. John Calise, Audrey G. O’Neill, Anika L. Burrell, Miles S. Dickinson, Josephine Molfino, Charlie Clarke, Joel Quispe, David Sokolov, Rubén M. Buey, Justin M. Kollmanthen with secondary antibodies diluted in PBS for 1 h at RT in the dark, and finally with 5 µM DAPI (ab228549; Abcam) in PBS for 20 min at RT in the dark. The slide was mounted with ProLong Glass Antifade Mountant

(138) Mitochondrial fusion and altered beta-oxidation drive muscle wasting in a Drosophila cachexia modelEMBO ReportsMarch 1, 2024Callum Dark, Nashia Ali, Sofya Golenkina, Vaibhav Dhyani, Ronnie Blazev, Benjamin L Parker, Kate T Murphy, Gordon S Lynch, Tarosi Senapati, S Sean Millard, et al.antibodies conjugated to Alexa 488 and Alexa 555 (Molecular Probes) were used at 1:200. DAPI 405 (Abcam, #ab228549) was used at 1:10,000, Phalloidin 647 (Abcam, #ab176759) was used at 1:10,000, HCS LipidTOXTM Red Neutral

(139) Nature-inspired peptide of MtDef4 C-terminus tail enables protein delivery in mammalian cellsScientific ReportsFebruary 26, 2024Lucia Adriana Lifshits, Yoav Breuer, Marina Sova, Sumit Gupta, Dar Kadosh, Evgeny Weinberg, Zvi Hayouka, Daniel Z. Bar, Maayan Galthe PFA. All samples were stained with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI Staining Solution ab228549, Abcam). Cav-1 (ab2910, Abcam), β-tubulin (05-661, Merck). Images were taken directly from the 96-well

(140) MiR-155 promotes compensatory lung growth by inhibiting JARID2 activation of CD34+ endothelial progenitor cellsPLOS ONEFebruary 23, 2024Li Zhao, Jing Peng, Li Zhuang, Zhiling Yan, Fei Liao, Yifan Wang, Shihao Shao, Weiwei Wangincubated for 1 hour at 37°C, and washed 3 times with PBS and then 1 time with distilled water. DAPI (ab228549, Abcam, UK) was added to the stained specimen, 50% buffered glycerol was added to mount the specimen,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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