DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(81) 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and FunctionalizationAdvanced Healthcare MaterialsFebruary 16, 2025Meghan A Robinson, Sonia HY Kung, Khaled YM Youssef, Kali M Scheck, Robert H Bell, Funda Sar, Anne M Haegert, M Mahdi Asmae, Changfeng Cheng, Salina V Yeack, et al.at room temperature. Cells were rinsed another 3 times. 4′,6‐diamidino‐2‐phenylindole (DAPI, Abcam, ab228549) was diluted to 2.5 µM in PBS and added to the cells for 15 min in the dark at room temperature, and

(82) Interleukin-1 prevents SARS-CoV-2-induced membrane fusion to restrict viral transmission via induction of actin bundleseLifeFebruary 12, 2025Zheng X, Yu S, Zhou Y, Yu K, Gao Y, Chen M, Duan D, Li Y, Cui X, Mou J, et al.(1:400) Chemical compound,Actin-Tracker Green-488,Beyotime,C2201S,IF (1:100) Chemical compound,DAPI,Abcam,ab228549,IF (1:2000) Chemical compound,Antifade mounting medium,Vectorlabs,H-1400-10,N/A Chemical compound,Purified

(83) Engineering of Genetically Encoded Bright Near-Infrared Fluorescent Voltage IndicatorInt J Mol SciFebruary 8, 2025Xian Xiao, Aimei Yang, Hanbin Zhang, Demian Park, Yangdong Wang, Balint Szabo, Edward S. Boyden, Kiryl D. Piatkevichslices were washed with PBS three times and incubated with DAPI (1:2000 dilution in PBS; Abcam, Cat#ab228549-2m, Waltham, MA, USA) and mounted with ProLong™ Gold Antifade (Thermo Fisher, Cat# P36930, St. Bend,

(84) Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury ModelJournal of Visualized ExperimentsFebruary 7, 2025Marta Stetsiv, Matthew Wan, Shagun Prabhu, Rosa Guzzo, Archana SanjayScientific,22-363-172, "Cryofilm Type II C(10), 3.5 cm","Section Lab, Japan",CFS 105, DAPI Staining Solution,Abcam,ab228549, Digital Gram Scale,Toprime/ Amazon,B06X6LW4V9,Can use any brand scale to weigh mice Dissecting Forceps,Fisherbrand/

(85) Pharmacological Inhibition of N-Acylethanolamine Acid Amidase (NAAA) Mitigates Intestinal Fibrosis Through Modulation of Macrophage ActivityJournal of Crohn's and ColitisFebruary 4, 2025Maria Francesca Nanì, Ester Pagano, Paola De Cicco, Giuseppe Lucariello, Fabio Cattaneo, Francesca Paola Tropeano, Donatella Cicia, Rebecca Amico, Federica Raucci, Giuseppe Ercolano, et al.microscope slides using Mounting Medium (Cat. #AB104135, Abcam) and then counterstained with DAPI (Cat. #AB228549, Abcam) for nuclear staining. The images were acquired at room temperature and detected under 40× magnification

(86) 22 and 50 kHz rat ultrasonic vocalization playback reveals sex differences in behavior and cFos in brain regions associated with affective processingBehavioural Brain ResearchFebruary 1, 2025Sydney M. Bonauto, Kaya A. Patel, Jennifer A. Honeycutt(ab6564, Abcam)). Tissue was washed 3 × 5 min in PBS, then incubated for 30 minutes in 1:1000 DAPI (ab228549, Abcam) in PBS. Finally, brain sections were washed 3 × 5 min, mounted in glass slides, and coverslipped

(87) Definitive Evidence for the Identification and Function of Renin-Expressing Cholinergic Neurons in the Nucleus AmbiguusHypertensionFebruary 1, 2025Éva M. Fekete, Javier Gomez, Mina Ghobrial, Kathren Kaminski, Patricia C. Muskus, Carie R. Boychuk, Ana Hantke Hantke Guixa, Ibrahim Vazirabad, Michelle Xie, Azeez Ganiyu, et al.,ACD Bio,310043 Chemicals,, Propranolol,Millipore-Sigma,P0884-5G Methyl-Atropine,Millipore-Sigma,M1300000 Phenylephrine hydrochloride,Millipore-Sigma,P6126-5G DAPI,Abcam,Ab228549 ,, Key resource table

(88) Unraveling the Microenvironment and the Pathogenic Axis of HIF‐1α–Visfatin–Fibrosis in Autoimmune Pancreatitis Using a Single‐Cell AtlasAdvanced ScienceJanuary 31, 2025Deyu Zhang, Congjia Ma, Zhen Wang, Yanfang Liu, Zaoqu Liu, Wanshun Li, Yue Liu, Chang Wu, Liqi Sun, Fei Jiang, et al.were incubated with multiple antibodies and counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI) (ab228549, Abcam, Cambridge, UK). Finally, the slides were observed under a fluorescence microscope. Cell Culture Human

(89) Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancerNat CommunJanuary 30, 2025Seunghyun Lee, Biancamaria Ricci, Jennifer Tran, Emily Eul, Jiayu Ye, Qihao Ren, David Clever, Julia Wang, Pamela Wong, Michael S. Haas, et al.(21112-1-AP)) followed by anti-rabbit-Alexa 488 secondary antibody (Abcam, ab150077, 1:1000) and DAPI (Abcam, ab228549) for an hour and mounted with anti-fade fluorescence mounting medium (Abcam). PyMT-mCherry tumor cells

(90) Intrathecal transplantation of human umbilical cord mesenchymal stem cells enhances spinal cord injury recovery: Role of miR‑124‑3p as a biomarkerExp Ther MedJanuary 22, 2025Yitong Zheng, Yongxin Wang, Wen Liu, Mujite A, Yabin Li, Xiaohu Ma, Mieradili Abulimiti, Nuerailijiang Maimaitiaili, Hu Qinincubation at 37˚C for 30 min. DAPI staining (dilution, 1:1,000; 10 min at room temperature; Abcam; cat. no. ab228549) was used to label cell nuclei. Following washing with PBS, the sections were observed and imaged under

(91) Rapid and scalable personalized ASO screening in patient-derived organoidsNatureJanuary 22, 2025John C. Means, Anabel L. Martinez-Bengochea, Daniel A. Louiselle, Jacqelyn M. Nemechek, John M. Perry, Emily G. Farrow, Tomi Pastinen, Scott T. Youngerantibody incubation, cardiac organoids were washed 5 × 3 min in 1X PBS. After secondary incubation, DAPI (AB228549, Abcam) was added at 1:1,000 for 15 min at room temperature, followed by three washes with 1X PBS. Antibody

(92) Enhancing Cytoplasmic Expression of Exogenous mRNA Through Dynamic Mechanical StimulationAdvanced Healthcare MaterialsJanuary 1, 2025Jiawen Chen, Aneri Patel, Mohammad Mir, Maria R. Hudock, Meghan R. Pinezich, Brandon Guenthart, Matthew Bacchetta, Gordana Vunjak‐Novakovic, Jinho Kimsolution (Cat. No. 30–2300) were purchased from ATCC. 4,6-diamidino-2-phenylindole (DAPI) (Cat. No. ab228549) was purchased from Abcam. EGFP mRNA-lipid nanoparticle (LNP) (Cat. No. PM-LNP-0021) was purchased from

(93) Small spheroids for head and neck cartilage tissue engineeringScientific ReportsDecember 30, 2024Sven Reutter, Johann Kern, Yvonne Jakob, Nicole Rotter, David Gvaramiamanufacturer’s instructions. Total cell nuclei were counterstained using a DAPI staining solution (ab228549, Abcam) (Figs. 1, 2, 3 and 4). Image analysis To analyze the viability, mitochondrial ATP staining,

(94) CD38 Coordinates with NF-κB to Promote Cochlear Inflammation in Noise-Induced Hearing Loss: the Protective Effect of ApigeninMolecular NeurobiologyDecember 27, 2024Da Liu, Na Sai, Ying Zhou, Ning Yu, Qing-qing Jiang, Wei Sun, Wei-ju Han, Weiwei Guo(Proteintech, 60,006–1-lg). Rinse thoroughly and seal the tablet with DAPI-containing glycerin (Abcam, ab228549). The specimens were observed by excitation light at 405 nm (blue), 488 nm (green), and 555 nm (red)

(95) Prion protein regulates invasiveness in glioblastoma stem cellsBMC cancerDecember 18, 2024Prado MB, Coelho BP, Iglesia RP, Alves RN, Boccacino JM, Fernandes CFL, Melo-Escobar MI, Ayyadhury S, Cruz MC, Santos TG, et al.anti-mouse (#A28175, Invitrogen) secondary antibodies at a 1:1000 dilution, along with DAPI (Abcam, ab228549) at 1:1000, all in PBS with 2% normal goat serum and 0.2% Triton X-100. Coverslips were then washed

(96) Wanted: Dead or Alive Cells with Propidium Iodide Staining in Liver TissueInternational Journal of Molecular SciencesDecember 17, 2024Tim Christopher Krapoth, Gina Sophie Henle, Mihrije Avdyli, Berina Bektić, Katharina Maria Schwarzkopf, Larisa Bešić, Stefan Zeuzem, Christoph Welsch, Nico Kraus, Cristina OrtizResource,Source,Location,Identifier "4’,6-Diamidin-2-phenylindol (DAPI)",Abcam,"Campbridge, United Kingdom",Ab228549 Acetone p.a.,Sigma-Aldrich,"Saint Louis, MO, USA",32201-1L Alexa Fluor 488 phalloidin,Invitrogen,"Carlsbad,

(97) Phospho-regulation of ASCL1-mediated chromatin opening during cellular reprogrammingDevelopmentDecember 15, 2024Roberta Azzarelli, Sarah Gillen, Frances Connor, Jethro Lundie-Brown, Francesca Puletti, Rosalind Drummond, Ana Raffaelli, Anna PhilpottTriton X100-PBS containing 2% serum. Cells were stained with 1 μg/ml DAPI staining solution (Abcam, ab228549) for 20 min at room temperature. Imaging and image analysis Fluorescent images were taken using the

(98) Development of a fibrin-targeted theranostic for gastric cancerScience Translational MedicineDecember 11, 2024Shadi A. Esfahani, Li Ma, Shriya Krishna, Hua Ma, Shvan J. Raheem, Sergey Shuvaev, Nicholas J. Rotile, Jonah Weigand-Whittier, Avery T. Boice, Nicholas Borges, et al.Cell Signaling Technology) at 1/1000 dilution and for nuclei using 4′,6-diamidino-2-phenylindole (DAPI) staining solution (ab228549, Abcam). Microscopic evaluation was performed with a BioTeK Citation 5 microscopy system. The biomarker

(99) Definitive Evidence for the Identification and Function of Renin-Expressing Cholinergic Neurons in the Nucleus AmbiguusHypertensionDecember 2, 2024Éva M. Fekete, Javier Gomez, Mina Ghobrial, Kathren Kaminski, Patricia C. Muskus, Carie R. Boychuk, Ana Hantke Guixa, Ibrahim Vazirabad, Michelle Xie, Azeez Ganiyu, et al.,ACD Bio,310043 Chemicals,, Propranolol,Millipore-Sigma,P0884-5G Methyl-Atropine,Millipore-Sigma,M1300000 Phenylephrine hydrochloride,Millipore-Sigma,P6126-5G DAPI,Abcam,Ab228549 ,, Key resource table

(100) Fullerenol C60(OH)40 Nanoparticles and Ectoine Protect Human Nasal Epithelial Cells Against the Cytokine Storm After Addition of the Full-Length Spike Protein from SARS-CoV-2International Journal of NanomedicineNovember 21, 2024Malwina Sosnowska, Mateusz Wierzbicki, Barbara Nasiłowska, Totka Nikolaeva Bakalova, Klara Piotrowska, Barbara Strojny-Cieślak, Ewa Sawosz, Marta Kutwinwere washed three times with PBS and then stained with 5 µM 4′,6-diamidino-2-phenylindole (DAPI, No. ab228549, Abcam) for 15 min. After rinsing with PBS, the Ibidi Mounting Medium (No. 50001, Animalab, Teltow,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

Abcam

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DAPI Staining Solution from Abcam

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