DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(21) SNCA triplication disrupts proteostasis and extracellular architecture prior to neurodegeneration in human midbrain organoidsnpj Parkinson's DiseaseFebruary 16, 2026Elpida Statoulla, Maria Zafeiri, Kleanthi Chalkiadaki, Georgia Voudouri, Karmel S. Gkika, Christos Papazoglou, Thomas M. Durcan, Arkady Khoutorsky, Seyed Mehdi Jafarnejad, Sarah Maguire, et al.detailed list of antibodies can be found in Sup. Data 4. DRAQ5 (Abcam, #ab108410) or DAPI (Abcam, #ab228549) were used for nuclear staining. Imaging was performed using a LEICA SP5 and a NIKON A1R HD confocal

(22) Cell jamming transition is regulated by mitochondrial pyruvate transport and endocytosisbioRxivFebruary 13, 2026Alexandra Bermudez, Zoe Latham, Johnny Diaz, Weihong Yan, Jerry Chen, Dapeng Bi, Andrew Goldstein, Jimmy K Hu, Neil Y.C. Linanti-rabbit Alexa Fluor 647 (Invitrogen, A21042 and 4414S, respectively)—together with DAPI (Abcam, ab228549) and either phalloidin (Invitrogen, A12379) or actin stain (Invitrogen, R37110). Microscopy and Live-cell Imaging

(23) Obesity Reprogrammes Adipose Extracellular Vesicles to Induce Muscle Atrophy via miR-150-5p-Mediated Transcriptional SilencingJ Cachexia Sarcopenia MuscleFebruary 3, 2026Joshua M. J. Price, Michael Macleod, Thomas Nicholson, Caitlin M. Ditchfield, Bethy Airstone, Natalie Lachlan-Jiraskova, Edward T. Davis, Kostas Tsintzas, Simon W. Jonesat RT. Wells were then washed with PBS, after which DAPI staining solution (1:5000 in PBS, Abcam, #ab228549) was added for 5 min in the dark. Finally, wells were washed again with PBS, before Fluoromount Aqueous Mounting Medium

(24) Astrocytogenic bidirectional plasticity at spinal nociceptive synapses regulates acute nociceptive processingPainFebruary 1, 2026Sibel Ada, Laura Klinger, Hannah L. Teuchmann, Valeria Mussetto, Viktoria Hadschieff, Mira T. Kronschläger, Anna S.M. Siegert, Lidia Trofimova, Raphael Holzinger, Danijela Kurija, et al.×1 PBS (3 × 10 minutes) and incubated in ×1 PBS + 0.2% 40,6-diamidino-2-phenylindole (DAPI, Abcam, ab228549), protected from light, for 60 minutes. Slices were again washed with ×1 PBS (2 × 5 minutes) and mounted

(25) AX-2: A Promising Non-Hemolytic Protein of Bacillus thuringiensis with Potent Selective Cytotoxicity Against Breast Cancer CellsMoleculesJanuary 29, 2026Alain Cruz-Nolasco, Miguel Angel Peña-Rico, Sibel J. Estrada-Escobedo, Angel A. Ortela-Gregorio, Erick A. Juarez-Arellano, Genaro Vázquez-Victorio, Angelica S. Martinez-Ramirez, Michele Rorato Sagrillo, Roberto C. Vianna Santos, Luz Camacho, et al.RT. Excess liquid was removed by aspirating around the sample. Next, 1 µg/mL DAPI staining solution (ab228549, Abcam, Cambridge, UK) was added and incubated for 5 min. Antifade mounting media was added and coverslips

(26) VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung developmentFrontiers in Cell and Developmental BiologyJanuary 23, 2026Antony Hoarau, Andrew Frauenpreis, Randa Belgacemi, Emma Loeffler, Osshaya Maalouf, Ian A. Glass, Denise Al Alam, Soula Danopoulos,Thermo Fisher,MA5-13188,1/100 Claudin-5,LSBio,LS-C352946,1/200 Ki67,Epredia,RM-9106-S1,1/200 DAPI,Abcam,AB228549,1/1000 RNAscope assay,RNAscope assay,RNAscope assay,RNAscope assay APLN-C3,ACDB,44–9971-C3,1/50 Secondary

(27) Optimal murine CD4+ T cell priming by mRNA-lipid nanoparticle vaccines requires endogenous antigen processingNature CommunicationsJanuary 16, 2026Julia E. Rood, Suh Kyung Yoon, Mary K. Heard, Stephen D. Carro, Emma J. Hedgepeth, Mary E. O’Mara, Michael J. Hogan, Nhu Le, Hiromi Muramatsu, Kieu Lam, et al.(Invitrogen A21131, diluted to 10 μg/mL) to boost NP signal, followed by staining with DAPI (Abcam AB228549, diluted 1:1000) for 5 minutes. Muscle sections were additionally stained with wheat germ agglutinin

(28) 3D immunolabelling of human aortic valves by optimized CUBIC tissue clearing and its application for neural mapping in aortic stenosisEuropean Heart Journal OpenJanuary 6, 2026Justine Bergeon, David Hupin, Jianjiang Hu, Sylvie Le Guyader, Anders Franco-Cereceda, Magnus Bäckconjugated to Alexa Fluor 647. Nuclear staining was performed using DAPI (1:15 000 dilution, Abcam, ab228549). α7nAchR were labelled with first with the primary antibody, anti-nicotinic acetylcholine receptor alpha 7

(29) Permeability-driven pressure and cell proliferation control lumen morphogenesis in pancreatic organoidsNature Cell BiologyJanuary 1, 2026Byung Ho Lee, Kana Fuji, Heike Petzold, Phil Seymour, Siham Yennek, Coline Schewin, Allison Lewis, Daniel Riveline, Tetsuya Hiraiwa, Masaki Sano, et al.4 °C. To stain nuclei, Hoechst solution (34580, Invitrogen) or 4,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) was added in 0.25% Triton, 1% BSA in PBS for 4 h at room temperature after the incubation with

(30) Multifunctional copper-based metal-organic frameworks/chitosan derivative/exosome composite sponge for skin healingiScienceDecember 19, 2025Heng Zhou, Jiaqi Wang, Yu Ding, Huan Liang, Tao Qin, Pengjiao Wang, Xiuli Gao, Xiaoqing HeLtd.",Cat# 01000433 BTC,"Titan Scientific Co., Ltd.",Cat# 011801124 DAPI,"Abcam Trading Co., Ltd.",Cat# ab228549 CS,"Shanghai Ron Biotechnology Co., Ltd.",Cat# R051510 TMG,"Titan Scientific Co., Ltd.",Cat# 01089722 EDC,"Titan

(31) Positive modulation of sigma-1 receptor: a new weapon to mitigate disease progression in amyotrophic lateral sclerosisTransl NeurodegenerDecember 15, 2025Julien Le Friec, Hugo Mourier, Simon Couly, Nicolas Cubedo, Kevin Dubois, Johann Meunier, Benjamin Delprat, Arnaud De Zordo-Banliat, Tahar Ayad, David Virieux, et al.Finally, sections were briefly washed with 1× TBS, incubated for 4 min with DAPI (dilution 1:30,000; ab228549; Abcam, Cambridge, UK) and washed in water. Slides were mounted using FluoroSaveTM Reagent (345789;

(32) Heat-shock pathway activation by TRC051384 protects spiral ganglion neurons from noise-induced hearing lossbioRxivDecember 11, 2025Jintao Yu, Miguel A. Ramirez, Yi-Zhi Wang, Seby Edassery, Maxwell Shramuk, Mary Ann Cheatham, Mark A. Rutherford, Leah J Welty, Jeffrey N Savasincubation, samples were washed three times in PBS (15 min each) and counterstained with DAPI solution (Abcam ab228549) for 5 min. Tissues were mounted using ProLong Gold Antifade Mountant (Thermo Scientific, Cat# P10144).

(33) Immunocompetent Murine Models Recapitulate the Heterogeneous Tumor-Immune Microenvironment of Human LiposarcomaClinical Cancer ResearchDecember 1, 2025Amanda M. Shafer, Emma Kenna, Lexi-Ann F. Golden, Ahmed M. Elhossiny, Kyle D. Perry, Jodi Wilkowski, Wei Yan, Brynn Kaczkofsky, Jake McGue, Scott C. Bresler, et al.Roche). See Supplementary Table S1 for antibody details. DAPI (4′,6-diamidino-2-phenylindole; Abcam, AB228549) was used to stain nuclei. mT/mG indicator mice samples were stained for GFP and tdTomato using the

(34) MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesisGenes & DevelopmentDecember 1, 2025Jonathon M. Muncie-Vasic, Tanvi Sinha, Alexander P. Clark, Emily F. Brower, Jeffrey J. Saucerman, Brian L. Black, Benoit G. Bruneauthe same blocking buffer with gentle rotation at 37°C. Secondary antibodies and 1 µg/mL DAPI (Abcam ab228549) were added in a blocking buffer consisting of PBS (Life Technologies 14190250), 5% normal donkey serum

(35) Loss of the RNA Binding Protein HuR in Early Murine Limb Mesenchyme Does Not Affect Development but Leads to Impaired Bone Homeostasis in AdulthoodThe FASEB JournalNovember 30, 2025Shijian Fu, Kirsty A. Johnson, Phaedra Winstanley‐Zarach, Ufuk Ersoy, Elena Adlmanninger, Benjamin T. McDermott, Craig Keenan, Aphrodite Vasilaki, Ioannis Kanakis, Peter I. Milner, et al.hairpin amplifier overnight at room temperature. The embryos' cell nuclei were then stained with DAPI (ab228549, Abcam) before imaging. HCR probe sets were designed following previously reported protocols [18, 19].

(36) Loss of the RNA Binding Protein HuR in Early Murine Limb Mesenchyme Does Not Affect Development but Leads to Impaired Bone Homeostasis in AdulthoodFASEB JNovember 20, 2025Shijian Fu, Kirsty A. Johnson, Phaedra Winstanley‐Zarach, Ufuk Ersoy, Elena Adlmanninger, Benjamin T. McDermott, Craig Keenan, Aphrodite Vasilaki, Ioannis Kanakis, Peter I. Milner, et al.hairpin amplifier overnight at room temperature. The embryos' cell nuclei were then stained with DAPI (ab228549, Abcam) before imaging. HCR probe sets were designed following previously reported protocols [18, 19].

(37) Altered Cell Surface N -Glycosylation Implicates Hypersialylation in Breast Cancer Brain MetastasisJournal of Proteome ResearchNovember 7, 2025Judith Nwaiwu, Wenjing Peng, Akhila Reddy, Xue Dong, Parisa Ahmadi, Jingfu Zhao, Yifan Huang, Waziha Purba, Peilin Jiang, Oluwatosin Daramola, et al.Paraformaldehyde was purchased from Fisher Scientific (Fair Lawn, New Jersey, USA). DAPI solution (Cat. no: AB228549) was acquired from Abcam (Waltham, MA, USA). Concanavalin A Alexa Fluor ™ 647 conjugate (Cat. no: C21421),

(38) Mitofusin 1 in mitochondrial quality control and anti-inflammatory responses in nucleus pulposus cells during disc degenerationScientific ReportsNovember 4, 2025Jae Won Shin, Young Mi Kang, Hak Sun Kim, Seong Hwan Moon, Kyung Soo Suk, Si Young Park, Byung Ho Lee, Ji Won Kwondilution (Invitrogen; Thermo Fisher Scientific) and 10 μM 4′,6-diamidino-2-phenylindole (DAPI; Abcam, ab228549, Milton, UK) for nuclear staining at room temperature (20–22 °C) for 1 h. The samples were examined

(39) Sex differences in response to kanamycin-induced ototoxicity in C57BL/6 J miceScientific ReportsOctober 31, 2025Natalia Smith-Cortinez, Louise V. Straatman, Ferry G. J. Hendriksen, Robert J. Stokroos, Huib Versnelantibodies donkey-anti-rabbit-Alexa 594 (1/500, Invitrogen, A-21207) and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Finally, the samples were washed in PBS and mounted in Vectashield

(40) Breaking the link between morphology and potency for mESCsCell BiosciOctober 23, 2025Yixin Fan, Xiaomin Wang, Ziwei Zhai, Tao Huang, Wei Li, Zechuan Liang, Zhaoyi Ma, Yu Fu, Pengli Li, Junqi Kuang, et al.(BBI) for 15 min at 37 °C. Washed the cells again with PBS, stained with DAPI stanning solution (Abcam, ab228549) for 10 min at room temperature. Images were captured with Confocal Microscope LSM 980 (Zeiss). Cell viability assay

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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