DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(41) Immunocompetent Murine Models Recapitulate the Heterogeneous Tumor-Immune Microenvironment of Human LiposarcomaClin Cancer ResSeptember 10, 2025Amanda M. Shafer, Emma Kenna, Lexi-Ann F. Golden, Ahmed M. Elhossiny, Kyle D. Perry, Jodi Wilkowski, Wei Yan, Brynn Kaczkofsky, Jake McGue, Scott C. Bresler, et al.Roche). See Supplementary Table S1 for antibody details. DAPI (4′,6-diamidino-2-phenylindole; Abcam, AB228549) was used to stain nuclei. mT/mG indicator mice samples were stained for GFP and tdTomato using the

(42) Isotope-encoded spatial biology identifies plaque-age-dependent maturation and synaptic loss in an Alzheimer’s disease mouse modelNature CommunicationsSeptember 1, 2025Jack I. Wood, Maciej Dulewicz, Alicja Szadziewska, Sophia Weiner, Junyue Ge, Katie Stringer, Sneha Desai, Lydia Fenson, Diana Piotrowska, Gunnar Brinkmalm, et al.(Thermo Fisher Scientific, #A-21209)). Nuclei were counterstained with 4ʹ,6-diamidino-2- phenylindole (Abcam, #ab228549) for 5 minutes at RT. For structural amyloid analysis, tissues were stained with two luminescent conjugated

(43) A Decoy Oligodeoxynucleotides Disturbing Forkhead Box O3 Mediated ctnna2 Transcriptional Repression Prevents Postoperative Neurocognitive Disorder in MiceCNS Neurosci TherAugust 26, 2025Zhixin Wu, Dongkun Xie, Jing Zhao, Jianshuai Zhao, Huiqing Liu, Dong Xing, Tingting Gu, Yaru Guo, Dan Wang, Zhihong Lu, et al.room temperature. The slides were washed and cover‐slipped with mounting media containing DAPI (Abcam, ab228549, 1:10000) for observation under a confocal laser scanning microscope (FV10, Olympus, Japan). 2.6.2

(44) Yes-Associated Protein (YAP) Is Associated With Disease Severity and Visual Prognosis in Epiretinal MembraneInvest Ophthalmol Vis SciAugust 18, 2025Hyun Seung Yang, Yong-Ha Jo, Dongwoo Kim, Suho Choi, Byung Gil Moon, Youngseop Lee, Minji Kim, Donghoon Koo, Jeongmin Kimstaining was performed by incubating tissue sections with 4′,6-diamidino-2-phenylindole (DAPI, 1:400, ab228549; Abcam, Cambridge, UK) for nuclear staining, along with primary antibodies against rabbit anti-COL1

(45) NXF1 suppresses progression of endometrial cancer by interacting with the SRSF3 to regulate SP4 splicingiScienceAugust 15, 2025Nan Meng, Lingjun Li, Xueqin Yang, Hui Tang, Jizhong Wang, Songbai Zhang, Zongbao Xia, Jia Yao, Qi Zhang, Changrong Hu, et al.and recombinant proteins" ,, Lipofectamine™ 2000,Invitrogen™,11668019 DAPI Staining Solution,Abcam,ab228549 Lipofectamine™ RNAiMAX,Thermo Fisher Scientific,13778030 Co-IP lysis buffer,Beyotime,P0038 protein A/G plus agarose

(46) GPD1L as a potential biomarker associated with Treg cell infiltration and lipid metabolism in clear cell renal cell carcinomaJ Transl MedAugust 6, 2025Ming Yang, Dejiang Pang, Chuhui Gong, Kangping Song, Hongbo Ma, Yu Yang, Shujin Guo, Liqiong Wangprocedures as previously reported [21, 22]. The antibodies and reagents used in this process, such as DAPI (#ab228549, Abcam, 1:1000), anti-CD4 (Rabbit, #ab133616, Abcam, 1:200), anti-CD25 (Rabbit, #ab9496, Abcam, 1:200),

(47) Ginsenoside Rk1 Enhances Chemosensitivity of Gastric Cancer Through Activating the AMPK/mTOR PathwayDose ResponseAugust 5, 2025Yaqin Huang, Jian Wu, Li Zheng, Gen Huang, Bin Li, Lihong Gan, Ling YaoBrdU (Cat.No. ab220074, Abcam, Cambridge, UK). 4′-6-diamidino-2-phenylindole (DAPI) solution (Cat.No. ab228549, Abcam, Cambridge, UK) was used to stain the nuclei for 5 minutes. Finally, a fluorescence microscope

(48) Transcriptome-wide regulation of folate on neural mRNA m6A methylome via carbon metabolism in Drosophila and mammalsCommun BiolAugust 4, 2025Anrui Liu, Zhengyi Huo, Zihe Xu, Sisi Liu, Yuting Jiao, Rui Li, Yi Lu, Meng Yang, Jia Huang, Chen Huang, et al.Invitrogen) for 1 h. The slides were mounted with nail polish and stained with DAPI Staining Solution (#ab228549, Abcam). A FLUOVIEW FV3000 confocal laser scanning microscope (Olympus, Shinjuku, Japan) was used to

(49) N6-methyladenosine (m6A) dysregulation contributes to network excitability in temporal lobe epilepsyJCI InsightJuly 22, 2025Justine Mathoux, Marc-Michel Wilson, Sujithra Srinivas, Gabrielle Litovskich, Leticia Villalba Benito, Cindy Tran, Jaideep Kesavan, Aileen Harnett, Theresa Auer, Amaya Sanz-Rodriguez, et al.buffer along with secondary antibodies. Coverslips were mounted using mounting medium with DAPI (Abcam, ab228549) and imaged using a Zeiss LSM 800 confocal microscope. For PSD95 and dendritic spine quantification,

(50) TNF‑α induces premature senescence in tendon stem cells via the NF‑κB and p53/p21/cyclin E/CDK2 signaling pathwaysInt J Mol MedJuly 10, 2025Hua Guo, Haixia Cao, Qian Lu, Zhifeng Gu, Guijuan FengAbcam] at 1:250 were added for 2 h at room temperature. Nuclei were counterstained with DAPI (cat. no. ab228549, Abcam) at 1:500 at room temperature for 30 min and cells were examined using a fluorescence microscope

(51) Paradoxical regulation of IGF2 in promoting lipid metabolism in adipose tissuesCommun BiolJuly 9, 2025Caiping Zhou, Weiwei Gui, Weifen Zhu, Hanxin Zhao, Dingting Wu, Fang Wu, Gangliang Wang, Xihua Linfluorescent (FITC-conjugated IgG) secondary antibodies. Nuclear staining was executed using DAPI (Abcam, ab228549). Three sections at least 160 μm apart were subjected to insulin immunohistochemistry staining and scanned

(52) Deciphering the tumor immune microenvironment: single-cell and spatial transcriptomic insights into cervical cancer fibroblastsJ Exp Clin Cancer ResJuly 5, 2025Zhiheng Lin, Youwei Zhou, Zhenran Liu, Wenyang Nie, Hengjie Cao, Shengnan Li, Xuanling Li, Lijun Zhu, Guangyao Lin, Yanyu Ding, et al.[ab150116, 1:500], Abcam) were applied for 1 h at RT in the dark. Nuclei were counterstained with DAPI (ab228549, 1:1000, Abcam) for 10 min before mounting with Fluoromount-G (SouthernBiotech, USA). Imaging was performed

(53) Intermediate cell states in spermatogonial stem cell differentiation: enhancing neurogenesis and angiogenesis in spinal cord injuryEur J Med ResJuly 2, 2025Xinyu Guo, Hao Yang, Haihong Zhanganti-mouse IgG antibody, 1:1000) incubation was performed at room temperature for 1 h. DAPI (1:1000; Abcam, ab228549) was added before microscopic observation. Fluorescent signals were documented using a dedicated fluorescence imaging

(54) Mucosal Mixed Lymphocyte Reaction Assay Using Intestinal Lymphocytes as a Biomarker for Intestinal Transplant Tolerance DevelopmentTransplantationJuly 1, 2025M. Esad Gunes, Satyajit Patwardhan, Julie Hong, Elin Manell, Philip Jordache, Ishit Chauhan, Ahmed Almesallmy, Jianing Fu, Megan Sykes, Joshua WeinerUSA, Cat: 124260250). 5μL of DAPI (10mM) diluted 1:10000 with wash buffer (Abcam, Waltham, MA, Cat: ab228549) was added immediately before acquisition to distinguish dead cells. Flow Cytometry Acquisition The

(55) Harnessing cell-encapsulated hydrogels to study astrocyte mechanoresponse in 4DbioRxivJune 27, 2025Ana N. Strat, Suhani Patel, Minh-Tri Ho Thanh, Alexander Kirschner, Souvik Ghosh, Michael P. Geiss, Mariano Viapiano, Yutao Liu, Alison E. Patteson, Samuel Herberg, et al.antibody to rabbit IgG, 1:500, Abcam, Ab150077) for 1 h; nuclei were counterstained with DAPI (Abcam, Ab228549). Sections were mounted with ProLong™ Gold Antifade (Thermo Fisher Scientific, P36930, Waltham, MA,

(56) Utidelone Suppresses PDAC Growth and Enhances Gemcitabine Therapy by Inducing Immunogenic Cell DeathiScienceJune 20, 2025Jingyi Zhou, Kangnan Zhang, Chuan Liu, Jiang Long, Haitao Gu, Hanguang Dong, Chuntao Wu, Ling Xu, Fan Wang, Qi LiCalf Serum,Sigma-Aldrich,F4135 ʟ-Glutamine–Penicillin–Streptomycin,Sigma-Aldrich,G6784 cell counting kit-8 (CCK-8) reagent,DOJINDO,CK04 DAPI,Abcam,ab228549 phorbol myristate acetate (50 ng/mL),Sigma-Aldrich,P8139 monensin (10 μg/mL),Sigma-Aldrich,475897 ionomycin

(57) Molecular signatures of regional vulnerability to tauopathy in excitatory cortical neuronsActa NeuropathologicaJune 7, 2025Diede W. M. Broekaart, Abhijeet Sharma, Aarthi Ramakrishnan, Anjalika Chongtham, Dorothee M. Günther, Saraswathi Subramaniyan, Minghui Wang, Vishwendra Patel, Bin Zhang, Lea T. Grinberg, et al.washed with PBS and incubated with 4’−6-diamidino-2-phenylindole (DAPI) staining solution (1:10.000, ab228549, Abcam) for 5 minutes before being coverslipped with Prolong Diamond Antifade Mountant (P36970, Invitrogen,

(58) Polystyrene nanoplastics trigger pyroptosis in dopaminergic neurons through TSC2/TFEB-mediated disruption of autophagosome-lysosome fusion in Parkinson’s diseaseJ Transl MedJune 5, 2025Xiaomei Liang, Yaqi Zeng, Piao Zhang, Baoyu Zhu, Jiezhu Feng, Tongtong Deng, Zhongling Fu, Chengshuai Liu, Chengyu Chen, Yuhu Zhangand Alexa Fluor 594-conjugated (A-11037, Thermo Fisher Scientific, USA) secondary antibodies. DAPI (ab228549, Abcam, UK) was then applied to stain nuclei. Fluorescence signals were visualized using a confocal

(59) KSR1 Mediates Small Cell Lung Carcinoma Tumor Initiation and Cisplatin ResistanceMolecular Cancer ResearchJune 3, 2025Deepan Chatterjee, Robert A. Svoboda, Dianna H. Huisman, Benjamin J. Drapkin, Heidi M. Vieira, Chaitra Rao, James W. Askew, Kurt W. Fisher, Robert E. LewisAssay For colony formation assays, SCLC cells were dissociated by gentle pipetting. Cells were DAPI (Abcam #ab228549) stained for viability determination and live GFP+/RFP+ cells were single cell sorted as one cell per

(60) KSR1 mediates small-cell lung carcinoma tumor initiation and cisplatin resistanceMol Cancer ResJune 3, 2025Deepan Chatterjee, Robert A. Svoboda, Dianna H. Huisman, Benjamin J Drapkin, Heidi M. Vieira, Chaitra Rao, James W. Askew, Kurt W. Fisher, Robert E. LewisAssay For colony formation assays, SCLC cells were dissociated by gentle pipetting. Cells were DAPI (Abcam #ab228549) stained for viability determination and live GFP+/RFP+ cells were single cell sorted as one cell per

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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