DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(221) pH-Responsive Polymer Nanoparticles for Efficient Delivery of Cas9 Ribonucleoprotein With or Without Donor DNAAdvanced materials (Deerfield Beach, Fla.)June 1, 2022Xie R, Wang X, Wang Y, Ye M, Zhao Y, Yandell BS, Gong S.stained by secondary antibodies (Abcam, ab150113, ab150080, ab150155, all 1:1,000) and DAPI (Abcam, ab228549, 1:1,000) for visualization. After mounting on glass slides, the sections were imaged using a Nikon

(222) Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension TestFrontiers in Behavioral NeuroscienceMay 31, 2022Liuchang Zhou, Dan Liu, Zedan Xie, Di Deng, Guoqi Shi, Jinlan Zhao, Shasha Bai, Lei Yang, Rong Zhang, Yafei Shifrozen sections with cryostats (Leica CM1860 UV, Germany). The electrode locations were reconstructed DAPI (ab228549, Abcam) staining and imaging on a confocal microscope (Leica SP8, Germany). The distribution of serotonergic neurons

(223) Targeting Adiponectin Receptor 1 Phosphorylation Against Ischemic Heart FailureCirc ResMay 25, 2022Di Zhu, Zhen Zhang, Jianli Zhao, Demin Liu, Lu Gan, Wayne Bond Lau, Dina Xie, Zhijun Meng, Peng Yao, Jumpei Tsukuda, et al.kit,Sigma-Aldrich,https://www.sigmaaldrich.com/US/en/product/sigma/duo94001 DAPI,Abcam,https://www.abcam.com/dapi-staining-solution-ab228549.html In-Fusion HD cloning kit,Takara,https://www.takarabio.com/search-results?term=in-Fusion+HD+cloning+kit&tab=product Superfect

(224) Microvascular Dysfunction in Skeletal Muscle Precedes Myocardial Vascular Changes in Diabetic Cardiomyopathy: Sex-Dependent DifferencesFrontiers in Cardiovascular MedicineMay 18, 2022Sadi Loai, Xuetao Sun, Mansoor Husain, Michael A. Laflamme, Herman Yeger, Sara S. Nunes, Hai-Ling Margaret Chengperformed on cardiac and skeletal tissues. Staining for CD31, αSMA (Abcam, ab220179), and DAPI (Abcam, ab228549) was conducted to identify arterioles and capillaries. CD31 was conjugated to Alexa Fluor 647 (Fisher,

(225) Post-treatment with propofol inhibits inflammatory response in LPS-induced alveolar type II epithelial cellsExperimental and therapeutic medicineApril 1, 2022Yang X, Ma L.secondary antibodies with incubation at 37˚C for 45 min. Nuclei were counterstained with DAPI (cat. no. ab228549; Abcam) and cells were visualized using confocal microscopy (magnification, x400; FV1000; Olympus Corporation). ELISA Cell culture

(226) A physiologically relevant, estradiol-17β [E2]-responsive in vitro tissue-engineered model of the vaginal epithelium for vaginal tissue researchNeurourology and urodynamicsApril 1, 2022Shafaat S, Mangir N, Chapple C, MacNeil S, Hearnden V.Abcam) for 1 h at RT and counterstained with 4′,6‐diamidino‐2‐phenylindole (λex 359 nm, λem 457 nm) (ab228549) (1:800 dilution; Abcam), mounted with DPX, and imaged with an epifluorescence microscope (Olympus IX73). 2.8.1

(227) Fibroblast growth factor 21 reverses high-fat diet-induced impairment of vascular function via the anti-oxidative pathway in ApoE knockout miceJournal of cellular and molecular medicineApril 1, 2022Huang WP, Chen CY, Lin TW, Kuo CS, Huang HL, Huang PH, Lin SJ.for 1 h. After 1 h, the samples were carefully washed with PBS and then stained with DAPI (1:1000; ab228549; Abcam, USA) for 15 min at room temperature. The cover glasses were mounted with mounting medium (Dako,

(228) Combined Prussian Blue Nanozyme Carriers Improve Photodynamic Therapy and Effective Interruption of Tumor MetastasisInternational Journal of NanomedicineMarch 25, 2022Wenhao Shen, Gaohua Han, Lei Yu, Song Yang, Xiangyi Li, Wei Zhang, Pei Peistained with Ki67 (A11390, Abclonal), Myc-Tag (AE070, Abclonal), anti-HIF-1α (ab179483, Abcam) and DAPI (ab228549, Abcam) antibodies at day 4. The c-Myc and HIF-1α levels of regulatory T cells in tumor cells were also

(229) Combined Prussian Blue Nanozyme Carriers Improve Photodynamic Therapy and Effective Interruption of Tumor MetastasisInt J NanomedicineMarch 25, 2022Wenhao Shen, Gaohua Han, Lei Yu, Song Yang, Xiangyi Li, Wei Zhang, Pei Peistained with Ki67 (A11390, Abclonal), Myc-Tag (AE070, Abclonal), anti-HIF-1α (ab179483, Abcam) and DAPI (ab228549, Abcam) antibodies at day 4. The c-Myc and HIF-1α levels of regulatory T cells in tumor cells were also

(230) Human umbilical cord mesenchymal stem cells contribute to the reconstruction of bladder function after acute spinal cord injury via p38 mitogen-activated protein kinase/nuclear factor-kappa B pathwayBioengineeredMarch 1, 2022Jue Li, Jiliang Huang, Ling Chen, Wei Ren, Wenzhi Caigoat anti-mouse IgG H&L (Alexa Fluor® 594, ab150120, Abcam, UK). Next, the nuclei were counterstained with DAPI Staining Solution (ab228549, Abcam, UK). The model group functioned as the negative control group. After the sections were mounted

(231) Imaging-guided bioreactor for de-epithelialization and long-term cultivation of ex vivo rat tracheaLab on a ChipMarch 1, 2022Seyed Mohammad Mir, Jiawen Chen, Meghan R. Pinezich, John D. O'Neill, Sarah X. L. Huang, Gordana Vunjak-Novakovic, Jinho KimS3). Lastly, the sections were washed, incubated with DAPI solution (4’,6-diamidino-2-phenylindole; ab228549, Abcam) for 15min, rewashed, and mounted with mounting medium (Permount™, Cat. No. SP15-100, Fisher

(232) ASCL1 phosphorylation and ID2 upregulation are roadblocks to glioblastoma stem cell differentiationScientific reportsFebruary 11, 2022Azzarelli R, McNally A, Dell'Amico C, Onorati M, Simons B, Philpott A.were incubated for two hours at room temperature. Cells were then stained with 1 μg/mL DAPI (Abcam ab228549) for 20 min at room temperature. Images were acquired with a confocal microscope (Zeiss invert LSM510

(233) EW-7197 Attenuates the Progression of Diabetic Nephropathy in db/db Mice through Suppression of Fibrogenesis and InflammationEndocrinology and metabolism (Seoul, Korea)February 1, 2022Ha KB, Sangartit W, Jeong AR, Lee ES, Kim HM, Shim S, Kukongviriyapan U, Kim DK, Lee EY, Chung CH.with cells for 1 hour at 22°C to 24°C. A 4′,6-diamidino-2-phenylindole (DAPI; 1 mg/mL stock solution; ab228549, Abcam, Cambridge, UK) was diluted to 1 mL/0.5 μL and incubated with cells for 15 minutes at 22°C to

(234) Crosstalk between the ancestral type VII secretion system ESX-4 and other T7SS in Mycobacterium marinumiScienceJanuary 21, 2022Wang Y, Tang Y, Lin C, Zhang J, Mai J, Jiang J, Gao X, Li Y, Zhao G, Zhang L, et al.and stained with Alexa fluor 488-conjugated phalloidin (Thermo scientific A12379) and DAPI (Abcam, ab228549) for 20 min following the protocol recommended by the manufacturer. Stained cells were protected with

(235) Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contractionCommunications biologyJanuary 20, 2022Fan T, Hou Y, Ge W, Fan T, Feng X, Guo W, Song X, Gao R, Wang J.Invitrogen, Cat#: A11037). The nuclei were stained with 4′,6-diamidino-2-phenylindole (Abcam, Cat#: ab228549). Images were photographed using a Leica optical microscope (Leica Microsystems, Germany). Real-time polymerase chain reaction

(236) SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egressScience advancesJanuary 7, 2022Scherer KM, Mascheroni L, Carnell GW, Wunderlich LCS, Makarchuk S, Brockhoff M, Mela I, Fernandez-Villegas A, Barysevich M, Stewart H, et al.meant for expansion microscopy were counterstained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam, ab228549) diluted 1:1000 in PBS for 15 min at RT and mounted on Fisherbrand glass microscope slides (Fisher Scientific,

(237) Acupuncture Ameliorates Depressive Behaviors by Modulating the Expression of Hippocampal Iba-1 and HMGB1 in Rats Exposed to Chronic Restraint StressFrontiers in psychiatryJanuary 1, 2022Chen L, Jiang H, Bao T, Wang Y, Meng H, Sun Y, Liu P, Quan S, Li W, Qi S, et al.five times with PBS. Detection of nucleus was performed with DAPI staining solution (1:1000, Abcam, ab228549, USA) and then incubated at room temperature for 5 min. The slides were washed with PBS for 3 times,

(238) Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-29a Improves Ovarian Function of Mice with Primary Ovarian Insufficiency by Targeting HMG-Box Transcription Factor/Wnt/β-Catenin SignalingDisease markersJanuary 1, 2022Gao T, Cao Y, Hu M, Du Y.sample was conducted using a fluorescence microscope (AxioCam HRc; ZEISS) following the DAPI staining (ab228549, Abcam). 2.5. UCMSC and GC Transwell Coculture To investigate the biological functions of HUCMSC-derived

(239) MicroRNA miR-24-3p Mediates the Negative Regulation of Lipopolysaccharide-Induced Endometrial Inflammatory Response by Targeting TNF Receptor-Associated Factor 6 (TRAF6)Journal of inflammation researchJanuary 1, 2022Oladejo AO, Li Y, Imam BH, Ma X, Shen W, Wu X, Jiang W, Yang J, Lv Y, Ding X, et al.secondary antibodies (1:300) in the dark box for 1hour at 37°C. Finally, the nuclei were stained using DAPI(ab228549, Abcam, China) for 10 min, washed with PBS, sealed with an anti‐fluorescence quencher, and captured

(240) ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory StressFrontiers in endocrinologyJanuary 1, 2022Chi A, Yang B, Cao X, Wang Z, Liu H, Dai H, Deng C, Zhang M.ab150080, 1:500). Alexa Fluor 594-conjugated goat anti-mouse (Abcam, ab150116, 1:500), DAPI (Abcam, ab228549, 1:1000). Images were acquired using a confocal microscope (LSM800, Zeiss). To investigate the testis

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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