DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(221) Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-29a Improves Ovarian Function of Mice with Primary Ovarian Insufficiency by Targeting HMG-Box Transcription Factor/Wnt/β-Catenin SignalingDisease markersJanuary 1, 2022Gao T, Cao Y, Hu M, Du Y.sample was conducted using a fluorescence microscope (AxioCam HRc; ZEISS) following the DAPI staining (ab228549, Abcam). 2.5. UCMSC and GC Transwell Coculture To investigate the biological functions of HUCMSC-derived

(222) Acupuncture Ameliorates Depressive Behaviors by Modulating the Expression of Hippocampal Iba-1 and HMGB1 in Rats Exposed to Chronic Restraint StressFrontiers in psychiatryJanuary 1, 2022Chen L, Jiang H, Bao T, Wang Y, Meng H, Sun Y, Liu P, Quan S, Li W, Qi S, et al.five times with PBS. Detection of nucleus was performed with DAPI staining solution (1:1000, Abcam, ab228549, USA) and then incubated at room temperature for 5 min. The slides were washed with PBS for 3 times,

(223) Blockage of Galectin-Receptor Interactions Attenuates Mouse Hepatic Pathology Induced by Toxoplasma gondii InfectionFrontiers in immunologyJanuary 1, 2022He J, Hou Y, Lu F.secondary antibody at room temperature for 60 min in a dark chamber, and then incubated with DAPI (ab228549, Abcam) for 30 min in a dark chamber. CD137+ cells were marked by green fluorescent signals. The sections

(224) MicroRNA miR-24-3p Mediates the Negative Regulation of Lipopolysaccharide-Induced Endometrial Inflammatory Response by Targeting TNF Receptor-Associated Factor 6 (TRAF6)Journal of inflammation researchJanuary 1, 2022Oladejo AO, Li Y, Imam BH, Ma X, Shen W, Wu X, Jiang W, Yang J, Lv Y, Ding X, et al.secondary antibodies (1:300) in the dark box for 1hour at 37°C. Finally, the nuclei were stained using DAPI(ab228549, Abcam, China) for 10 min, washed with PBS, sealed with an anti‐fluorescence quencher, and captured

(225) Smooth muscle cells affect differential nanoparticle accumulation in disturbed blood flow-induced murine atherosclerosisPLOS ONEDecember 9, 2021Hunter A. Miller, Morgan A. Schake, Badrul Alam Bony, Evan T. Curtis, Connor C. Gee, Ian S. McCue, Thomas J. Ripperda, Yiannis S. Chatzizisis, Forrest M. Kievit, Ryan M. Pedrigirabbit at 1:750, Abcam #ab150079). Both immunostains were counterstained with DAPI (0.0025%, Abcam #ab228549). Immunostained-sections were imaged at 20x magnification with a confocal microscope. Confocal parameters

(226) The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instabilityCell reportsDecember 7, 2021Ruggiano A, Vaz B, Kilgas S, Popović M, Rodriguez-Berriguete G, Singh AN, Higgins GS, Kiltie AE, Ramadan K.,Santa Cruz Biotechnology,Cat#sc-284628 Hoechst 33258,Sigma-Aldrich,Cat#B2883 DAPI staining solution,Abcam,Cat#ab228549 Propidium Iodide,Sigma-Aldrich,Cat#P4864 KaryoMAX Colcemid solution,ThermoFisher Scientific,Cat#15212012 Proteinase K

(227) Cytotoxicity of snake venom Lys49 PLA2-like myotoxin on rat cardiomyocytes ex vivo does not involve a direct action on the contractile apparatusScientific ReportsDecember 1, 2021Alfredo Jesús López-Dávila, Natalie Weber, Theresia Kraft, Faramarz Matinmehr, Mariela Arias-Hidalgo, Julián Fernández, Bruno Lomonte, José María GutiérrezThermo Fisher, Dreieich, Germany). Nuclei were stained with DAPI according to the manufacturer´s instructions (ab228549; Abcam, Berlin, Germany). Fluorescent images were acquired using an Olympus IX51 microscope and processed

(228) The TRPA1 Channel Amplifies the Oxidative Stress Signal in MelanomaCellsNovember 11, 2021De Logu F, Souza Monteiro de Araujo D, Ugolini F, Iannone LF, Vannucchi M, Portelli F, Landini L, Titiz M, De Giorgi V, Geppetti P, et al.Sections were coverslipped using a water-based mounting medium with 4′6′-diamidino-2-phenylindole (DAPI, #ab228549, Abcam). Negative controls were performed in tissue samples collected from the analyzed cases, in which

(229) Clinically relevant preservation conditions for mesenchymal stem/stromal cells derived from perinatal and adult tissue sourcesJournal of Cellular and Molecular MedicineNovember 1, 2021Anh T. L. Ngo, Hang M. Le, Nhung T. H. Trinh, Adriel Peng Guo Jun, Trung Q. Bach, Hue T. H. Bui, Van T. Hoang, Anh V. Bui, Liem T. Nguyen, Duc M. Hoangwere fixed with 4% paraformaldehyde (PFA) and stained with DAPI staining solution (1:5000, Abcam; #ab228549). The DAPI signals were captured using the ImageXpress® Micro Confocal High‐content Imaging System (Molecular Devices

(230) Clemastine improves electrophysiologic and histomorphometric changes through promoting myelin repair in a murine model of compression neuropathyScientific reportsOctober 22, 2021Lee JI, Park JW, Lee KJ, Lee DH.antibody (1:200, ab31851; Abcam) in 5% BSA. Incubation with 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, ab228549; Abcam) and fluorescent secondary antibodies (Alexa Fluor 488, and 594-conjugated antibodies [1:500,

(231) Treatment With Melatonin After Corneal Graft Attenuates RejectionFrontiers in PharmacologyOctober 19, 2021Ziqian Zhu, Ruiping Peng, Hongyi Shen, Lei Zhong, Siqi Song, Tao Wang, Shiqi Ling(Cell signaling Technology, Germany, 1:1,000) for 1 h at room temperature, followed by DAPI (Abcam, Ab228549) solution staining. Photographs were taken with a fluorescence microscope (DM 4000B). The number of

(232) LGR5-Positive Supporting Cells Survive Ototoxic Trauma in the Adult Mouse CochleaFrontiers in Molecular NeuroscienceOctober 5, 2021Natalia Smith-Cortinez, Rana Yadak, Ferry G. J. Hendriksen, Eefje Sanders, Dyan Ramekers, Robert J. Stokroos, Huib Versnel, Louise V. StraatmanInvitrogen, A-21207), donkey-anti Goat-Alexa 488 (1/200, Abcam, AB150129), and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Lastly, specimens were washed in PBS and mounted in Vectashield Antifade

(233) The molecular mechanism underlying mitophagy-mediated hippocampal neuron apoptosis in diabetes-related depressionJournal of cellular and molecular medicineAugust 1, 2021Liu J, Liu L, Han YS, Yi J, Guo C, Zhao HQ, Ling J, Wang YH.paraformaldehyde for 30 minutes and then incubated with 4′,6‐diamidino‐2‐phenylindole (DAPI) (1:800, ab228549, Abcam, USA) at room temperature for 15 minutes. Autophagosome, autolysosome and autophagic flux were

(234) Lamina-specific properties of spinal astrocytesGliaJuly 1, 2021Kronschläger MT, Siegert ASM, Resch FJ, Rajendran PS, Khakh BS, Sandkühler J.always covered with foil. Afterwards, slices were incubated in a DAPI‐staining solution (20 μM; Abcam ab228549) for 2 hr on a shaker. Slices were washed ×3 in 1× PBS for 15 min before mounting on glass sides using

(235) Photodynamic Therapy for Benign Cutaneous Neurofibromas Using Aminolevulinic Acid Topical Application and 633 nm Red Light IlluminationPhotobiomodulation, Photomedicine, and Laser SurgeryJune 1, 2021Brendan Quirk, Edit Olasz, Suresh Kumar, Donald Basel, Harry Whelan(DeadEnd Colorimetric TUNEL System, Promega, Madison, WI), and DAPI (4′,6-diamidino-2-phenylindole) staining (ab228549; Abcam, Cambridge, MA). Additional follow-up visits for measurement of lesions were made at periods

(236) Recombinant human bone morphogenetic protein-4 enhances tendon-to-bone attachment healing in a murine model of rotator cuff tearAnnals of Translational MedicineMay 14, 2021Huabin Chen, Zhanwen Wang, Li Zhou, Bing Wu, Hongbin Lu, Ciliu Zhang, Tao ZhangUK) at room temperature for 1 hour, and finally incubated with 4’,-diamidino-2-phenylindole (DAPI) (ab228549, Abcam). All images were observed and captured using a Zeiss Axio Imager (Zeiss, Solms, Germany). According

(237) High Glucose Attenuates Cardioprotective Effects of Glucagon-Like Peptide-1 Through Induction of Mitochondria Dysfunction via Inhibition of β-Arrestin-SignalingFrontiers in PhysiologyMay 13, 2021Xietian Pan, Chengxiang Li, Haokao GaoUnited States 15596018 MEBSTAIN Apoptosis TUNEL kit Takara, Japan MK500 DAPI Abcam, United Kingdom ab228549 Caspase-3 assay kit Clontech, CA 630217 ROS assay kit Abcam, United Kingdom ab113851 CCK-8 assay

(238) Uridine Diphosphate Promotes Rheumatoid Arthritis Through P2Y6 ActivationFrontiers in PharmacologyApril 19, 2021Hongxing Wang, Hui Wu, Kehua Fang, Xiaotian Changab150078) for 1 h in the dark. Nuclei were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI) (Abcam, ab228549). Images were acquired under a fluorescence microscope, and quantification of the signal density was

(239) Abnormal Glu/mGluR_2/3/PI3K pathway in the hippocampal neurovascular unit leads to diabetes-related depressionNeural regeneration researchApril 1, 2021Liu J, Han YS, Liu L, Tang L, Yang H, Meng P, Zhao HQ, Wang YH.SA00003-2; Proteintech) at 37°C for 30 minutes, and stained with 4′,6-diamidino-2-phenylindole (1:800; Cat# ab228549; Abcam) at room temperature for 15 minutes. A high-content analysis system was used to analyze protein expression

(240) Lipid Rafts Interaction of the ARID3A Transcription Factor with EZRIN and G-Actin Regulates B-Cell Receptor SignalingDiseases (Basel, Switzerland)March 20, 2021Schmidt C, Christian L, Smith TA, Tidwell J, Kim D, Tucker HO.goat anti-mouse IgG H&L (Alexa Fluor® 488; ab150117, Abcam), and stained with DAPI (Staining Solution ab228549, Abcam). Visualization is described below and shown in Figure 2B and Figure S4. Diseases 2021, 9,

Write a Review

(1)

6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

Abcam

Citations (276)

Reviews

DAPI Staining Solution from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map