GoTaq Probe 1-Step RT-qPCR System A6121 from Promega

Product Specs
ItemGoTaq Probe 1-Step RT-qPCR System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6121
Quantity12.5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Exploring the timeline and network interplay of immune mediators in COVID-19 patients according to disease outcomeFront ImmunolMarch 16, 2026Gabriel Macedo Costa Guimarães, Christiane Costa-Pereira, Renan da Silva Faustino, Lilian Santos Alves, Fabiana Rabe Carvalho, Thalia Medeiros, Joaquim Pedro Brito-de-Sousa, Laurence Rodrigues Amaral, Vanessa Peruhype-Magalhães, Ana Carolina Campi-Azevedo, et al.Integrated DNA Technologies, IDT, USA), in combination with the GoTaq® Probe 1-Step RT-qPCR System (Catalog #A6121, Promega Corporation, USA). Reactions were run on the 7500 Real-Time PCR System (Applied Biosystems,

(2) GW-9662, a peroxisome proliferator-activated receptor γ (PPARγ) inhibitor, impairs early embryonic development in zebrafishComparative Biochemistry and Physiology Part C: Toxicology & PharmacologyDecember 1, 2025Sunil Sharma, Kanchaka Senarath Pathirajage, Tyler Johnson, Jabez Raju Battu, Subham Dasguptatranscription polymerase chain reaction (RT-qPCR) The GoTaq® 1-Step RT-qPCR kit from Promega (CAT no. A6121) and Bio-Rad CFX real-time PCR detection system were used to perform RT-qPCR for measuring gene expression

(3) Portable molecular diagnostic platform for rapid point-of-care detection of mpox and other diseasesNat CommunMarch 24, 2025Matthew L. Cavuto, Kenny Malpartida-Cardenas, Ivana Pennisi, Marcus J. Pond, Sohail Mirza, Nicolas Moser, Mark Comer, Isobel Stokes, Lucy Eke, Sian Lant, et al.origin of the sample and the quality of the extraction. The Promega GoTaq Probe 1-step RT-qPCR (Cat. No. A6121, Promega) was used at a final volume of 20 µL per reaction following this protocol: 2x GoTaq qPCR master mix

(4) At what cycle threshold level are dogs able to detect SARS-CoV-2 in humans?PLoS OneJanuary 17, 2025Víctor M. Vidal-Martínez, Juan Manuel Mancilla-Tapia, Lilia C. Soler-Jiménez, Iván Velázquez-Abunader, Matilde Jiménez-Coello, Antonio Ortega-Pacheco, David Hernández-MenaSARS-CoV-2 virus and a human control gene (RP). The enzyme used was GoTaq® Probe 1-Step RT-qPCR System, A6121 (Promega Corporation, Wisconsin, USA). All nasopharyngeal samples were processed using a CFX Opus 96

(5) Evidence of SARS-CoV-2 infection in companion animals from owners who tested positive for COVID-19 in the Valley of MexicoMolecular Biology ReportsDecember 1, 2024Edith A. Fernández-Figueroa, Deborah V. Espinosa-Martínez, Haydee Miranda-Ortiz, Erika Ruiz-García, Juan M. Figueroa-Esquivel, Miriam L. Becerril-Moctezuma, Anallely Muñoz-Rivas, César A. Ríos-Muñozprobes (nucleocapsid protein gene) were included through the GoTaq Probe 1-Step RT-qPCR System (Cat. A6121, Promega) in a reaction volume of 20 μL containing: 10 μL GoTaq, 1.5 μL probe (N1, N2 or RNAse P), 0.4

(6) SARS-CoV-2 Genomic Variants and Their Relationship with the Expressional and Genomic Profile of Angiotensin-Converting Enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2)MicroorganismsNovember 14, 2024Henrique Borges da Silva Grisard, Marcos André Schörner, Fernando Hartmann Barazzetti, Julia Kinetz Wachter, Vilmar Benetti Filho, Rafael Emmanuel Godoy Martinez, Christinni Machado Venturi, Gislaine Fongaro, Maria Luiza Bazzo, Glauber Wagnercontrol to validate the reaction. For amplification, together with GoTaq® Probe 1-Step RT-qPCR (Cat. No. A6121, Promega, Madison, Wisconsin, USA®), specific primers and probes, i.e., PrimePCRTM Probe Assay: ACE2,

(7) Comparative Bioinformatic Analysis Reveals Conserved Regions in SARS-CoV-2 Genome for RAPID Pandemic ResponseInternational Journal of Molecular SciencesMay 25, 2024Marcela Viviana Karpuj, D. R. Shaytov, Yonat Shemer-Avni, Michael Gideon, Zakharia M. Frenkel, Sarit Sivansynthetic RNA fragment employing the GoTaq® Probe 1-Step RT-qPCR System from Promega (Catalog # A6120 and A6121). It is important to note that our in-silico analysis (illustrated in Figure 1, steps 1–3) resulted

(8) DeepSARS: simultaneous diagnostic detection and genomic surveillance of SARS-CoV-2BMC GenomicsDecember 1, 2022Alexander Yermanos, Kai-Lin Hong, Andreas Agrafiotis, Jiami Han, Sarah Nadeau, Cecilia Valenzuela, Asli Azizoglu, Roy Ehling, Beichen Gao, Michael Spahr, et al.performing RT-qPCR of a virus specific amplicon (N1) with the GoTaq® Probe 1- Step RT-qPCR System (Promega, A6121) based on methods from CDC. DeepSARS sequencing library preparation RT was performed using 1 μL of

(9) A quantitative RT‐qLAMP for the detection of SARS‐CoV ‐2 and human gene in clinical applicationMicrobial BiotechnologyOctober 1, 2022Yejiong Yu, Johnny X. Y. Zhou, Binbin Li, Mengmeng Ji, Yun Wang, Emma Carnaby, Monique I. Andersson, Wei E. Huang, Zhanfeng Cuisample were obtained. qPCR assay and digital PCR assay We used the GoTaq Probe one‐step RT‐qPCR kit (A6121; Promega) with a 1.5 μl primer mix to assess the C t values of the samples. CDC N1 primer set (1006770;

(10) The investigation of diverse physiological and therapeutic impact of cellular-based products derived from human cumulus cellsTurkish Journal of BiologySeptember 19, 2022DERYA BURUKÇU, SEMA YILMAZ, OYA ALAGÖZ, CUMHUR KAAN YALTIRIK, NECİP ERKUT ATTAR, FİKRETTİN ŞAHİN, ESRA AYDEMİR Aydemirtemperature of 60 °C. HNPCs were also characterized by gene expression using Taqman PCR master mix (#A6121, Promega, USA) and performed by a conventional PCR method according to the manufacturer’s protocol.

(11) Acute kidney injury associated to COVID-19 leads to a strong unbalance of circulant immune mediatorsCytokineSeptember 1, 2022Thalia Medeiros, Gabriel Macedo Costa GuimarÃ£es, Fabiana Rabe Carvalho, Lilian Santos Alves, Renan Faustino, Ana Carolina Campi-Azevedo, Vanessa Peruhype-MagalhÃ£es, AndrÃ©a Teixeira-Carvalho, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, et al.Integrated DNA Technologies, Inc - IDT, Iowa, USA) and the GoTaq® Probe 1-Step RT-qPCR (Catalog no. A6121, Promega Corporation, Wisconsin, USA). Amplification was performed using the 7500 System (Applied Biosystems,

(12) SARS-CoV-2 infects an in vitro model of the human developing pancreas through endocytosisiScienceJuly 15, 2022Szlachcic WJ, Dabrowska A, Milewska A, Ziojla N, Blaszczyk K, Barreto-Duran E, Sanak M, Surmiak M, Owczarek K, Grzanka D, et al.Reverse Transcription Kit,Thermo Fisher,Cat# 4368814 GoTaq Probe 1-Step RT-qPCR System,Promega,Cat# A6121 Human XL Cytokine Luminex Performance Assay Fixed Panel,R&D,Cat# LKTM014 DreamTaq Green PCR Master Mix (2X

(13) No molecular evidence of SARS‐CoV‐2 infection in companion animals from Veracruz, MexicoTransboundary and Emerging DiseasesJuly 1, 2022Sokani Sánchez‐Montes et al.N1 and N2 (Cat. 10006770. 2019‐nCov CDC EUA Kit, IDT), using GoTaq Probe 1‐Step RT‐qPCR System (Cat. A6121, Promega) in a reaction volume of 20 μl containing: 10 μl GoTaq, 1.5 μl probe (N1, N2), 4 μl GoScript,

(14) Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronavirusesCell chemical biologyMay 19, 2022Napolitano V, Dabrowska A, Schorpp K, Mourão A, Barreto-Duran E, Benedyk M, Botwina P, Brandner S, Bostock M, Chykunova Y, et al.AM1560 Crystallization kit,Natrix HT,Cat# HR2-131 GoTaq Probe 1-Step RT-qPCR System,Promega,Cat# A6121 ,, Deposited data,Deposited data,Deposited data ,, PLpro – ACF structure,This paper; Protein Data

(15) Marine Natural Products as Leads against SARS-CoV-2 InfectionJournal of Natural ProductsMarch 25, 2022Bhuwan Khatri Chhetri, Philip R. Tedbury, Anne Marie Sweeney-Jones, Luke Mani, Katy Soapi, Candela Manfredi, Eric Sorscher, Stefan G. Sarafianos, Julia KubanekN2, and RP (IDT, Coralville, IA, USA). Reactions were prepared with the GoTaq Probe RT-qPCR system (#A6121, Promega, Madison, WI, USA) in a final volume of 10 μL, with 1 μL of extracted RNA, and run on a PicoReal

(16) Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNAJournal of Virological MethodsSeptember 1, 2021William G. Dundon, Tirumala B.K. Settypalli, Katharina Spiegel, Adi Steinrigl, Sandra Revilla-Fernández, Friedrich Schmoll, Ivancho Naletoski, Charles E. Lamien, Giovanni Cattolireferred to below as the WHO Recommended Protocol (WRP). 2.4.5 GoTaq ® 1-Step RT-qPCR (Promega, Cat. #A6121) A 20 μL PCR final reaction volume contained 10 μL of 2X Reaction Mix, 0.5 μL of LightMix® Modular

(17) Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection programPLOS ONEAugust 18, 2021Haydee Miranda-Ortiz et al.human gene (Cat. 10006770. 2019-nCov CDC EUA Kit, IDT), using GoTaq Probe 1-Step RT-qPCR System (Cat. A6121, Promega) in a reaction volume of 20 μL containing: 10 μL GoTaq, 1.5 μL probe (N1, N2 or RNAse P), 0.4

(18) Smartphone screen testing, a novel pre-diagnostic method to identify SARS-CoV-2 infectious individualseLifeJune 22, 2021Rodrigo M Young et al.temperature and 3.3µl aliquots were used for RT- 331 PCR using Promega GoTaq Probe 1-Step RT-qPCR system (A6121) according to the manufacturer 332 instructions and supplemented with SARS-CoV-2 N2 probes and primers

(19) Serum ferritin at admission in hospitalized COVID-19 patients as a predictor of mortalityThe Brazilian Journal of Infectious DiseasesMarch 15, 2021Katia Lino, Gabriel Macedo Costa GuimarÃ£es, Lilian Santos Alves, Any Caroline Oliveira, Renan Faustino, Cintia Souza Fernandes, Gleiser TupinambÃ¡, Thalia Medeiros, Andrea Alice da Silva, Jorge Reis Almeida10006770, Integrated DNA Technologies, Inc – IDT, Iowa, USA) and GoTaq® Probe 1-Step RT-qPCR (Catalog no. A6121, Promega Corporation, Wisconsin, USA) the assay was performed in three separate reactions per specimen

(20) Rapid processing of SARS-CoV-2 containing specimens for direct RT-PCRPLOS ONEFebruary 10, 2021Piotr Chomczynski, Peter W. Chomczynski, Amy Kennedy, Michal Rymaszewski, William W. Wilfinger, Judith A. Heiny, Karol Mackeycat. no. 95133–500). GoTaq® Probe 1-Step RT-qPCR System (Promega Corporation, Madison WI, USA; cat. no A6121). KiCqStart® Probe qPCR ReadyMix™, ROX™ (MilliporeSigma, St. Louis, MO, USA; cat. no. KCQS06). The RT-qPCR

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GoTaq Probe 1-Step RT-qPCR System

GoTaq Probe 1-Step RT-qPCR System from Promega

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GoTaq Probe 1-Step RT-qPCR System from Promega

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