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LDH-Glo Cytotoxicity Assay
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LDH-Glo Cytotoxicity Assay
LDH-Glo Cytotoxicity Assay
LDH-Glo Cytotoxicity Assay from Promega
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Citations:
(197)
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LDH-Glo Cytotoxicity Assay
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Description
A simple, bioluminescence-based method to determine cytotoxicity by measuring lactate dehydrogenase (LDH) released into culture media
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
LDH-Glo Cytotoxicity Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
J2380
Quantity
10ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (197)
(1)
Non-destructive transcriptomics via vesicular export
Nat Commun
April 25, 2026
Niklas Armbrust, Martin Grosshauser, Julian Geilenkeuser, Luisa Stroppel, Mattea Jozinovic, Hella Levermann, Tobias Panne, Jannick Wißmann, Lukas Goelitz, Sebastian Schmidt,
et al.
CellTiter-Glo 2.0
Cell Viability Assay
(
Promega
,
G9241
), as well as
LDH-Glo Cytotoxicity Assay
(
Promega
,
J2380
), was performed in triplicate according to the manufacturer’s protocol 48 hours post-induction. Characterization
(2)
Impact of dipeptidyl peptidase I and neutrophil serine proteases on neutrophil functional responses
Frontiers in Pharmacology
April 24, 2026
Dedong Li, Kuan-Ju Chen, Nandita Niranjan, Jessica Basso, Daniel LaSala, Mei-Fong Pang, Craig Smith, Tam Nguyen, Vanessa de Carvalho Oliveira, David C. Cipolla,
et al.
activity in
lavage
fluid supernatants was quantified using the
LDH-Glo Cytotoxicity Assay
(
Promega
#
J2381
) according to the manufacturer’s instructions. Clarified supernatants were added to white 96-well plates,
(3)
New Mechanistic Evidence for Perfluorodecanoic Acid (PFDA) Teratogenicity via CYP26A1-Mediated Retinoic Acid Metabolism and Signaling
Chemical Research in Toxicology
April 20, 2026
Michaela Hvizdak, Sylvie E. Kandel, Jed N. Lampe
protocols. Cytotoxicity was assessed morphologically and via the
LDH-Glo cytotoxicity-assay
kit (catalog no.
J2380
) from
Promega
(Madison, WI). Recombinant CYP
In Vitro
atRA Oxidation Protein concentration and
reaction time
(4)
Translational strategy to support the first-in-human study of a TCR-like T cell bispecific with an in vitro-based safety approach
Front Immunol
April 17, 2026
Angelique Augustin, Blandine Avignon, Christophe Boetsch, Ekaterina Breous-Nystrom, Olaf Broders, Lauriane Cabon, Karen Dernick, Evelyne Durr, Miro Julian Eigenmann, Susanne Fischer,
et al.
release Target
cell lysis
was quantified via LDH release using the
LDH-Glo Cytotoxicity Assay
(Cat:
J2380
,
Promega
) or
Caspase 3
/7 cleavage using the
CellEvent Caspase-3/7 Green Detection Reagent
(Cat:
C10423
,
(5)
A protocol to establish and maintain organotypic cerebellar slice culture (OCerSC) from aged mice
PLoS One
April 17, 2026
Michael F. Almeida, Kaitlan Smith, Michael A. Garris, Rebekah Sanchez-Hodge, Meagan Colie, Jonathan C. Schisler
serving as a dependable marker of cytotoxicity [32]. We used the
LDH-Glo™ Cytotoxicity Assay
(
Promega
,
J2380
), a luminescent test that
measures
LDH activity through linked enzymatic reactions, ultimately producing
(6)
Specific inhibition of NLRP3 inflammasome by a Smurf1 inhibitor in vitro and in vivo
Open Med (Wars)
April 9, 2026
Lifeng Meng, Hongmei Xuan, Ping Zhou, Weifeng Xu, Wei Bian, Yunxiao Lin, Ying Guan
LDH level in the
cell culture
supernatants was measured by LDH Cytotoxicity Assay Kit (
Promega
, Cat #
J2380
) following the manufacturer’s instructions.
Immunoblot
analysis The cells were lysed with
cell lysis
(7)
Human Assembloid Model of Emergent Neurotropic Enteroviruses
bioRxiv
April 8, 2026
Christine E. Peters, Jimena Andersen, Min-Yin Li, Lauren Varanese, Mayuri Vijay Thete, Se-Jin Yoon, Taylor Pio, Nicholas Thom, Xiaoyu Chen, Wenjie Qiao,
et al.
lactate dehydrogenase (LDH) released into the
cell culture
supernatant using
LDH-Glo Cytotoxicity Assay
(
J2381
;
Promega
). Luciferase readings were taken one hour after reagent addition and read using a
GloMax 20/20 Luminometer
(8)
ATP13A2 Loss of Function-Driven Polyamine Dysregulation Induces SAM Depletion and Epigenetic Astrocyte Toxicity
bioRxiv
April 6, 2026
Elena Coccia, Gustavo Morrone Parfitt, Sundas Ijaz, Aisha Sati, Julia Gesner, Andrea Perez Arevalo, Jennifer Strong, Anna Bright, Soha Sohail, Thea Meimoun,
et al.
Cytotoxicity Assay To quantify cell death and cytotoxicity, the LDG-Glo™ Cytotoxicity Assay (
Promega
, #
J2380
) was employed. Briefly, 2μl of conditioned media was harvested and diluted in 298μl of storage buffer
(9)
Human Nasal Cells in Nanofibrillar Cellulose Hydrogel: Viability, Function, and Implications for Bone Tissue Regeneration
Cells
April 2, 2026
Marijana Sekulic, Alina Korah, Simona Negoias, Daniel Bodmer, Vesna Petkovic
reference. 2.3. Cytotoxicity Assay The
LDH-Glo™ Cytotoxicity Assay
(
Promega
, Madison, WI, USA; cat. #
J2381
) was used to assess cytotoxicity by quantifying lactate dehydrogenase (LDH) released from damaged cells
(10)
Breathing lung-on-chip: a versatile tool for assessing respiratory toxicity across multiple therapeutic modalities
Archives of Toxicology
April 1, 2026
Linnea Johansson, Giulia Raggi, James Cartwright, Johnny Lindqvist, Laurène Froment, Patrik Andersson, Catherine Betts, Jorrit J. Hornberg, Nina Hobi, Anna Ollerstam,
et al.
vehicle. Lactate dehydrogenase (LDH) analysis LDH activity was assessed using the LDH-Glo
assay
kit (
J2380
,
Promega
). Medium was sampled on days 1 and 3 for SA treatments, and on days 1, 3, 8 for RA in 2D experiments,
(11)
“Seipin mediates Perilipin-1 recruitment to lipid droplets to preserve human adipocyte identity”
bioRxiv
March 30, 2026
Denise Zhong, Ioanna Stavrakaki, Anand Desai, Tiffany DeSouza, Sabine Linskey, Shannon Joyce, Courtney Hatton, Danna Garcia, Reyan Kassam, Gregory Hendricks,
et al.
in storage buffer. LDH activity was then measured using the
Promega
LDH-Glo™ Cytotoxicity Assay
Kit (
J2381
) according to the manufacturer’s instructions. In vivo
adipose tissue
development
Progenitor cells
(12)
Human NLRP3 inflammasome activation leads to formation of condensate at the microtubule organizing center
Sci Adv
March 27, 2026
Jue Wang, Man Wu, Le Xiao, Gang Du, Muyuan Chen, Venkat G. Magupalli, Peter D. Dahlberg, Hao Wu, Grant J. Jensen
supernatants were analyzed for LDH activity using the
LDH-Glo Cytotoxicity Assay
Kit (
Promega
, catalog no.
J2381
) according to the manufacturer’s guidelines.
Immunofluorescence
and live-cell imaging
To detect protein
(13)
Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications
Advanced Science
March 17, 2026
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu,
et al.
LDL
Assay
LDH‐Glo cytotoxicity assay
was performed according to manufacturer's protocol (
Promega
,
J2380
). Briefly, 25 µL of sample media per well was transferred to a white 96‐well plate (Greiner Bio‐One,
(14)
Autophagy Activators Normalize Aberrant Tau Proteostasis and Rescue Synapses in Human Familial Alzheimer's Disease iPSC‐Derived Cortical Organoids
Advanced Science
March 1, 2026
Sergio R. Labra, Jadon Compher, Akhil Prabhavalkar, Mireya Almaraz, Claudia Cedeño Kwong, Christine Baal, Maria Talantova, Nima Dolatabadi, Julian Piña‐Sanz, Yubo Wang,
et al.
assays
Papain Dissociation System
,
Worthington Biochemical Corp
.,
LK003150
LDH‐Glo Cytotoxicity Assay
,
Promega
,
J2381
Chromium Next GEM Single Cell 3’ Kit v3.1,10x Genomics,1000269 Chromium Next GEM Chip G Single Cell
(15)
Hydrogen sulfide rescues IFNγ/TNFα-induced intestinal epithelial barrier dysfunction by enhancing oxidative phosphorylation
Scientific Reports
February 23, 2026
Janaíne Prata de Oliveira, Matthias van Sligtenhorst, Cansu Akkaya, Astrid Verbiest, Victoria Francelino Alves, Marie Wallays, Schalk van der Merwe, Marc Ferrante, Tim Vanuytsel, John L. Wallace,
et al.
cell culture medium
. The toxic concentration was determined by employing the
LDH-Glo cytotoxicity assay
(
Promega
; cat.
J2380
). In short, Caco-2/TC7 cells were exposed to a range of sodium azide concentrations (3 mM – 300 mM in
(16)
Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profiles
Cell Reports Methods
February 23, 2026
Marie Sejberg Øhlenschlæger, Pia Jensen, Jesper Foged Havelund, Sissel Ida Schmidt, Fadumo Abdullahi Mohamed, Magdalena Sutcliffe, Sofie Blomberg Elmkvist, Lucrezia Criscuolo, Steven W. Wingett, Ilaria Chiaradia,
et al.
assays
,Critical commercial
assays
,Critical commercial
assays
,,
LDH-Glo™ Cytotoxicity Assay
,
Promega
,
J2380
ROS-Glo® H2O2 Assay
,
Promega
,
G8820
Seahorse XF Real-Time ATP Rate
Assay
Starter Pack,Agilent,103677–100 Seahorse
(17)
Circulating Clues in Ménière’s Disease: Elevated Cell-Free DNA and a Pro-Inflammatory Signature in Patients’ Blood
International Journal of Molecular Sciences
February 18, 2026
Marijana Sekulic, Swethiny Kobivasan, Stavros Giaglis, Daniel Bodmer, Vesna Petkovic
Assay Cytotoxicity was assessed using the
LDH-Glo™ Cytotoxicity Assay
(
Promega
, Madison, WI, USA, cat#
J2381
) according to the manufacturer’s instructions. Briefly, 2.5 µL of conditioned medium from treated or
(18)
Untargeted Metabolomics of Human Airway Epithelium Reveals Neuroactive Signatures Linked to Pulmonary Neuroendocrine Cell Enrichment and Allergen Exposure
Metabolites
February 17, 2026
Ritu Mann-Nüttel, Ayshna Diya, Paul Forsythe
Assay
LDH activity in the basolateral supernatants was measured using the
LDH-Glo Cytotoxicity Assay
(#
J2380
,
Promega
, Madison, WI, USA). For each sample, 10 μL of basolateral media was collected and diluted
1:100
(19)
Rift Valley fever virus activates multiple cell death pathways in neurons
Journal of Virology
February 17, 2026
Kaleigh A. Connors, Zachary D. Frey, Matthew J. Demers, Morgan Midgett, Connor Williams, Douglas S. Reed, Zachary P. Wills, Amy L. Hartman
(
Invitrogen
,
15596026
). Cell viability Neuronal viability was assayed using the LDH-Glo
assay
(
Promega
,
J2380
) as described in the manufacturer’s instructions. Briefly, for the quantification of LDH, supernatant
(20)
MicroRNA Signatures of Prostate Cancer Spheroids in Microfluidic Culture Under Hormone-Deprivation Conditions
Bioengineering (Basel)
February 11, 2026
Kamaldeep Saini, Theresa Kordaß, Zita Zena, Martin Burchardt, Cindy Roennau, Pedro Caetano Pinto
Assay
,Type,Supplier,Reference LDH release,Luminescent,
Promega
,
J2380
Caspase 3/7,Luminescent,
Promega
,
G8090
Presto Blue,Colorimetric,
Invitrogen
,
A13261
PSA,ELISA,
R&D Systems
,
DY1344
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LDH-Glo Cytotoxicity Assay from Promega
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