LDH-Glo Cytotoxicity Assay J2380 from Promega

Product Specs
ItemLDH-Glo Cytotoxicity Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ2380
Quantity10ml

Add to Compare List

Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Preclinical assessment of a novel human antibody VH domain targeting mesothelin as an antibody-drug conjugateMolecular Therapy - OncolyticsDecember 19, 2023Zehua Sun, Xiaojie Chu, Cynthia Adams, Tatiana V. Ilina, Michel Guerrero, Guowu Lin, Chuan Chen, Dontcho Jelev, Rieko Ishima, Wei Li, et al.trials. Cell viability assays Cell viability was measured using CellTiter-Glo (G7570, Promega) or LDH-Glo (J2380, Promega). Briefly, MSLN-positive or -negative cells were plated into 96 wells, allowing attachment

(2) Single-Cell Transcriptomics and In Vitro Lineage Tracing Reveals Differential Susceptibility of Human iPSC-Derived Midbrain Dopaminergic Neurons in a Cellular Model of Parkinson's DiseaseCellsDecember 18, 2023Lucia F. Cardo, Jimena Monzón-Sandoval, Zongze Li, Caleb Webber, Meng Libuffer. LDH detection reagent and quantification were performed following the manufacturer’s instruction (J2380, Promega, Hampshire, UK). The essay was carried out using 3 technical replicates per culture well. Luminescence

(3) Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicityFront ImmunolDecember 8, 2023Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin, et al.from co-culture of these cells with NK cells using the LDH-Glo™ Cytotoxicity Assay kit (Promega, Cat.: J2380) according to the manufacturer’s instructions. 2.12 Analysis of cytokine concentration in cell culture

(4) INPP5D regulates inflammasome activation in human microgliaNature CommunicationsNovember 29, 2023Vicky Chou, Richard V. Pearse, Aimee J. Aylward, Nancy Ashour, Mariko Taga, Gizem Terzioglu, Masashi Fujita, Seeley B. Fancher, Alina Sigalov, Courtney R. Benoit, et al.cytotoxicity, LDH-Glo Cytotoxicity Assay was used as per the manufacturer’s instructions (Promega, J2380). For INPP5D overexpression, iMGs were transduced with either control or INPP5D OE (Origene, RC212176L1V;

(5) Lower cardiotoxicity of CPX-351 relative to daunorubicin plus cytarabine free-drug combination in hiPSC-derived cardiomyocytes in vitroScientific ReportsNovember 29, 2023Marie C. Fortin, Andrew S. LaCroix, Tom N. Grammatopoulos, Lei Tan, Qi Wang, Dino MancaGraphPad Prism as relative luminescence units. LDH release The LDH-Glo Cytotoxicity Assay (Promega, J2380) was used to assess LDH release from hiPSC-CM cultures according to the manufacturer’s guidelines. Briefly,

(6) Infection of liver sinusoidal endothelial cells with Muromegalovirus muridbeta1 involves binding to neuropilin-1 and is dynamin-dependentFrontiers in Cellular and Infection MicrobiologyNovember 9, 2023Ingelin Kyrrestad, Anett Kristin Larsen, Javier Sánchez Romano, Jaione Simón-Santamaría, Ruomei Li, Karen Kristine Sørensencytotoxicity assay Release of LDH to medium was analyzed with the Promega LDH Glo Cytotoxic assay (Cat. No J2380). LSECs (0.5 x 106 cells/well) in fibronectin-coated 24-well tissue culture plates (Sarstedt) were maintained

(7) Anti-tumor activity of a recombinant measles virus against canine lung cancer cellsScientific reportsOctober 24, 2023Tamura K, Fujiyuki T, Moritoh K, Akimoto H, Iizuka K, Sato H, Asano K, Yoneda M, Kai C.co-culture, cytotoxicity assay was performed by using LDH-Glo Cytotoxicity Assay kit (Promega, Cat#J2381). The cytotoxicity was calculated as described in the manufacturer’s protocol. Analysis of gene expression

(8) Inflammasome-mediated glucose limitation induces antibiotic tolerance in Staphylococcus aureusiScienceOctober 20, 2023Jenna E. Beam, Nikki J. Wagner, Kuan-Yi Lu, Joshua B. Parsons, Vance G. Fowler, Sarah E. Rowe, Brian P. Conlonassays ,, Caspase-Glo 1 Inflammasome Assay,Promega,Cat# G9951 LDH-Glo Cytotoxicity Assay,Promega,Cat# J2380 CellTiter-Blue Cell Viability Assay,Promega,Cat# G8080 Glucose Uptake-Glo,Promega,Cat# J1341 Seahorse

(9) FSH and ApoE4 contribute to Alzheimer’s disease-like pathogenesis via C/EBPβ/δ-secretase in female miceNature CommunicationsOctober 18, 2023Jing Xiong, Seong Su Kang, Mengmeng Wang, Zhihao Wang, Yiyuan Xia, Jianming Liao, Xia Liu, Shan-Ping Yu, Zhaohui Zhang, Vitaly Ryu, et al.C586, anti-AT8, anti-Tau N368 and anti-ApoE. The supernatants were collected for LDH assay (Promega J2380). Differentiation of human iPSC-derived NSCs into neurons NSCs derived from human induced pluripotent stem cells

(10) Technical report: liquid overlay technique allows the generation of homogeneous osteosarcoma, glioblastoma, lung and prostate adenocarcinoma spheroids that can be used for drug cytotoxicity measurementsFrontiers in Bioengineering and BiotechnologyOctober 6, 2023Camille Jubelin, Javier Muñoz-Garcia, Denis Cochonneau, Emilie Ollivier, François Vallette, Marie-Françoise Heymann, Lisa Oliver, Dominique Heymannadded to 15 µL of LDH Storage buffer and 25 µL Enzyme Mix from the LDH-Glo™ Cytotoxicity Assay kit (J2380, Promega, Charbonnières-les-Bains, France). Each sample was put in the well of an opaque white 96-well

(11) MARCH5-dependent NLRP3 ubiquitination is required for mitochondrial NLRP3-NEK7 complex formation and NLRP3 inflammasome activationThe EMBO journalOctober 4, 2023Park YJ, Dodantenna N, Kim Y, Kim TH, Lee HS, Yoo YS, Heo J, Lee JH, Kwon MH, Kang HC, et al.Health,https://imagej.nih.gov/nih‐image/ Biorender,N/A,https://biorender.com/ Ubpred,N/A,http://ubpred.org/ Other,Other,Other LDH‐Glo™ Cytotoxicity Assay,Promega,Cat# J2380 Caspase‐Glo® 1 Inflammasome Assay,Promega,Cat# G9951l Lipofectamine 2000,Invitrogen,Cat# 11668‐019 ELISA

(12) Tumor microenvironment in a minipig model of spinal cord gliomaJournal of Translational MedicineSeptember 27, 2023Muhibullah S. Tora, Stewart G. Neill, Yuliya Lakhina, Hemza Assed, Michelle Zhang, Purva P. Nagarajan, Thais Federici, Juanmarco Gutierrez, Kimberly B. Hoang, Yuhong Du, et al.evaluated for total ATP and LDH release using ATP Assay Kit (ab83355, ABCAM, USA) and LDH assay kits (J2380, Promega Corporation, USA) according to the protocols and read using a microplate reader (BioTek Instruments

(13) Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer’s disease tauCell & BioscienceSeptember 18, 2023Lenka Hromadkova, Chae Kim, Tracy Haldiman, Lihua Peng, Xiongwei Zhu, Mark Cohen, Rohan de Silva, Jiri G. SafarATP disodium salt hydrate (Millipore Sigma) as an inter-plate control. LDH-Glo Cytotoxicity Assay (J2380, Promega) measures the amount of LDH released to media by a bioluminescent method as an indication of

(14) Preclinical Characterization and Phase I Trial Results of INBRX-109, A Third-Generation, Recombinant, Humanized, Death Receptor 5 Agonist Antibody, in ChondrosarcomaClinical Cancer ResearchAugust 15, 2023Vivek Subbiah, Sant P. Chawla, Anthony P. Conley, Breelyn A. Wilky, Anthony Tolcher, Nehal J. Lakhani, David Berz, Vasily Andrianov, William Crago, Monica Holcomb, et al.dehydrogenase release was measured with the Bioluminescent LDH Release Toxicity Assay Kit (Promega, Catalog no. J2380), both according to InSphero AG internal technical operation procedures. The IC50, 95% CI, and Hill

(15) NS1 and PA-X of H1N1/09 influenza virus act in a concerted manner to manipulate the innate immune response of porcine respiratory epithelial cellsFrontiers in Cellular and Infection MicrobiologyJuly 26, 2023Robin Avanthay, Obdulio Garcia-Nicolas, Gert Zimmer, Artur Summerfieldlevel was monitored in the supernatant using the LDG-Glo-Cytotoxicity assay kit (Promega; cat. no. J2380) according to the manufacturer’s instructions. 2.9 Statistics Data analysis and figures were done

(16) Atezolizumab Induces Necroptosis and Contributes to Hepatotoxicity of Human HepatocytesInternational journal of molecular sciencesJuly 20, 2023Endo Y, Winarski KL, Sajib MS, Ju A, Wu WJ.dehydrogenase (LDH) assay. The LDH assay was performed using LDH-Glo cytotoxicity assay (Promega, cat# J2381, Madison, WI, USA) according to the manufacturer’s instruction. Luminescence was detected using plate

(17) Cisplatin exhibits superiority over MMC as a perfusion agent in a peritoneal mesothelioma patient specific organoid HIPEC platformScientific ReportsJuly 19, 2023Steven D. Forsythe, Richard A. Erali, Nicholas Edenhoffer, William Meeker, Nadeem Wajih, Cecilia R. Schaaf, Preston Laney, Cristian D. Vanezuela, Wencheng Li, Edward A. Levine, et al.and analyzed using Graph Pad Prism© v.8 (Graphpad, La Jolla, CA). LDH-GLO viability assay LDH-GLO (J2381; Promega) cytotoxicity assay was performed by preserving culture medium from post-treatment timepoints

(18) Per- and polyfluoroalkyl substances (PFAS) in mixtures show additive effects on transcriptomic points of departure in human liver spheroidsToxicological SciencesJune 28, 2023Gregory C Addicks, Andrea Rowan-Carroll, Anthony J F Reardon, Karen Leingartner, Andrew Williams, Matthew J Meier, Ivy Moffat, Richard Carrier, Luigi Lorusso, Barbara A Wetmore, et al.exclusion criteria Cytotoxicity was determined using a lactate dehydrogenase (LDH) assay (LDH-Glo, Promega J2380, Madison, Wisconsin), as per the manufacturer’s instructions. Briefly, at the end of PFAS exposures

(19) The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells' Differentiation StageBrain sciencesJune 4, 2023Jagečić D, Petrović DJ, Šimunić I, Isaković J, Mitrečić D.Detection Kit For the purposes of evaluating cellular damage, LDH-GloTM Cytotoxicity Assay (Promega, J2380, Madison, WI, USA) was used. Firstly, 5 µL of the medium was mixed with 95 µL of a storage buffer (200

(20) Fibroblast growth factor 21 attenuates ventilator-induced lung injury by inhibiting the NLRP3/caspase-1/GSDMD pyroptotic pathwayCritical CareMay 22, 2023Peng Ding, Rui Yang, Cheng Li, Hai-Long Fu, Guang-Li Ren, Pei Wang, Dong-Yu Zheng, Wei Chen, Li-Ye Yang, Yan-Fei Mao, et al.dehydrogenase (LDH) in the cell culture supernatant was measured by an LDH cytotoxicity assay kit (J2380, Promega). FGF21 administration Male C57BL/6 mice (6–8 weeks old, 20–24 g) were randomly divided into

Be the First to Write a Review!

LDH-Glo Cytotoxicity Assay

LDH-Glo Cytotoxicity Assay from Promega

Description

Promega

Citations (71)

Reviews

LDH-Glo Cytotoxicity Assay from Promega

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map