LDH-Glo Cytotoxicity Assay J2380 from Promega

Product Specs
ItemLDH-Glo Cytotoxicity Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ2380
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

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Website: http://www.promega.com

(1) Targeted Clearance of Senescent Endothelium by GPNMB-Specific CAR-T CellsJ Immunol ResAugust 5, 2026Peijie Yu, Jie Du, Yan Liu, Mei Zhoudehydrogenase (LDH) released by injured target cells was determined using the LDH Cytotoxicity Assay Kit (J2380, Promega) according to the manufacturer’s instructions. Specific lysis was calculated as ([experimental

(2) Protocol to study inflammasome activation in induced pluripotent stem cell-derived macrophagesSTAR ProtocJuly 28, 2026Chloe M. McKee, Melanie Cranston, Emma C. McKay, Mohammad Arefian, Ben C. Collins, Rebecca C. Collassays ,, Cytotoxicity Detection Kit Roche,Sigma-Aldrich,11644793001 LDH-Glo Cytotoxicity Assay,Promega,J2381 Human IL-1 beta/IL-1F2 DuoSet ELISA,R&D Systems,DY201 Human TNF-alpha DuoSet ELISA,R&D Systems,DY210 Human IL-18 ELISA kit

(3) Automation of 3D liver spheroid generation and acetaminophen dose-response on the MO:BOT enhances assay robustness and precisionSci RepJuly 21, 2026Dana Hellmold, Daniel S. Ziemianowicz, Frowin Ellermann, Philipp Depperschmidt, Max Appold, Ahmed S. Omar, Jonathan Kurz, Frank Krieg-Schneider, Thorben Pascal Hoppe, David Hackenberger, et al.with APAP from both handling conditions was analyzed using the LDH-Glo Cytotoxicity Assay (Promega, J2380) following the manufacturer’s instructions. Cell culture plates were equilibrated to RT for approximately

(4) Binding to Albumin and Off-Target Toxicity Confound the Use of LRRC8/VRAC Channel Blockers in Cell Physiology AssaysbioRxivJuly 19, 2026Mateo A. Boulos, Aayan M. Afghan, Alena Rudkouskaya, Antonio M. Fidaleo, Harmaan S. Sidhu, Meraj T. Khan, Alexander A. Monginassay To assess the cytotoxic effects of VRAC inhibitors, we used the LDH-Glo Cytotoxicity Assay (cat. #J2380, Promega, Madison, WI). Cells were plated in 96-well plates at a density of 8,000 cells per well and

(5) Maturation is required to model ischemia-reperfusion injury in engineered human cardiac tissuesFront Bioeng BiotechnolJuly 13, 2026Trevor R. Nash, Roberta I. Lock, Vanessa Yi Ran Li, Morgan J. Lamberti, Youngbin Kim, Eloy Sanchez, Sharon Fleischer, Daniel Naveed Tavakol, Gordana Vunjak-Novakovicquantify the amount of LDH present in the tissue supernatant using LDH-Glo Cytotoxicity Assay (Promega, J2380), carried out following manufacturer’s instructions. Statistical analysis All analyses were conducted

(6) Ready-to-use 3D bioprinted scaffolds from natural materials loaded with patient's PRGF for personalized skin regenerationiScienceJune 19, 2026Lidia Maeso, Eduardo Anitua, Roberto Tierno, Mohammad Hamdan Alkhraisat, Edurne Alonso, Jon Luzuriaga, Jon Zarate, Felipe Goñi, Tatiane Eufrásio-da-Silva, Mohammadsadegh Nadimifar, et al.viability/cytotoxicity kit,Fisher Scientific,Cat# L3224 Lactate dehydrogenase (LDH) cytotoxicity assay,Promega,Cat# J2380 Fastin™ Elastin kit,Biocolor,F2000 Sircol™ Collagen kit,Biocolor,S2000 ,, Experimental models: Cell

(7) Glimpse into the role of Kupffer cells in a spheroid model of metabolic dysfunction-associated steatohepatitis (MASH)Biology OpenJune 15, 2026Niklas Bogovic, Maria Drießlein, Marie Keller, Hans J. Schlitt, Edward K. Geissler, Lydia Schneider, Philipp Kreiner, Henrik Junger, Elke Eggenhoferviability Cell damage was assessed using the LDH-Glo™ Cytotoxicity Assay (Promega, Madison, WI, USA, cat. J2380). The supernatants were diluted 1:25 before analysis. Immunostaining All images were captured using

(8) Targeting ATF4 overcomes dual resistance to chemo-immunotherapy in gastric cancer by disrupting the DNA damage responseJ Immunother CancerJune 8, 2026Huili Ye, Wentao Zhang, Hongtai Cao, Zhiyi Peng, Tong Xu, Zhenhua Xia, Long Li, Na He, Yali Zhou, Junjie Qin, et al.measuring lactate dehydrogenase (LDH) release using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (#J2381, Promega) according to the manufacturer’s instructions. Briefly, target cells were seeded at 1×10⁵ cells/mL

(9) Bridging the gap: organotypic models to study late-onset group B streptococcus infectionMicrobiology SpectrumJune 2, 2026Alexia N. Pearah, Nichol John-Lewis Edwards, Rico R. Carter, Leigh-Anne M. Worthington, Madison R. Huszar, Kristen Domínguez, Hannah L. Wapshott-Stehli, April K. Lindon, Sophie E. Darch, Thao T. B. Ho, et al.measure the release of lactate dehydrogenase using the LDH-Glo Cytotoxicity assay kit (Promega Cat #J2380) and was followed according to the manufacturer’s instructions. To determine the amount of translocated

(10) A precision-cut lung slice platform for evaluating respiratory virus replication dynamicsbioRxivMay 29, 2026Jovanna A. Fusco, Min Liu, Devra Huey, Emily M. King, Amanda R. Panfil, Kara N. Corps, Ian C. Davis, Andrew S. Bowman, Cody J. Warrenmock-infected wells. Following the manufacturer’s instructions (LDH-Glo™ Cytotoxicity Assay, Promega, Cat# J2380), 2 μL of culture medium was collected from three replicate wells at 1-, 24-, 72-, and 96-hour timepoints

(11) A multi-agent system for automating scientific discoveryNatureMay 19, 2026Ali Essam Ghareeb, Benjamin Chang, Ludovico Mitchener, Angela Yiu, Caralyn J. Szostkiewicz, Dmytro Shved, Gavin J. Gyimesi, Jon M. Laurent, Samantha M. Wright, Muhammed T. Razzak, et al.assay was conducted according to the manufacturer’s protocol using the LDH-Glo Cytotoxicity Assay (J2381, Promega). To measure the maximal LDH activity, a positive control was created by lysing cells with

(12) De novo design of a macrocycle-induced dimerization system for cellular controlNature CommunicationsMay 18, 2026Stephanie Hanna, Patrick J. Salveson, Basile Wicky, Madison A. Kennedy, Derrick R. Hicks, Carolina Moller, Suna Cheng, Xinting Li, Mohamad Abedi, Brian Coventry, et al.Fig. 5). We also assessed the cytotoxicity of MC1 in HEK293T cells using an LDH-Glo assay (Promega J2380). HEK293T cells treated with MC1 titrated 1 in 2 from 50 uM to 50 nM and aliquots of cell media were

(13) In vitro modeling of renal injury-induced cardiac effects using human iPSC-derived organoidsCell Communication and SignalingMay 6, 2026Beatrice Gabbin, James Gallant, Fangchen Liu, Hailiang Mei, Berend J. van Meer, Ton J. Rabelink, Christine L. Mummery, Jessica M. Vanslambrouck, Cathelijne W. van den Berg, Viviana Meraviglia, et al.opaque white 96-wells plate (Costar, Cat. No: 3912). The LDH-Glo™ Cytotoxicity Assay (Promega, Cat. No: J2380) was also performed, where LI-BPEL culture supernatant and the kit’s reagent were mixed in a 1:1 ratio

(14) Non-destructive transcriptomics via vesicular exportNat CommunApril 25, 2026Niklas Armbrust, Martin Grosshauser, Julian Geilenkeuser, Luisa Stroppel, Mattea Jozinovic, Hella Levermann, Tobias Panne, Jannick Wißmann, Lukas Goelitz, Sebastian Schmidt, et al.CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9241), as well as LDH-Glo Cytotoxicity Assay (Promega, J2380), was performed in triplicate according to the manufacturer’s protocol 48 hours post-induction. Characterization

(15) Impact of dipeptidyl peptidase I and neutrophil serine proteases on neutrophil functional responsesFrontiers in PharmacologyApril 24, 2026Dedong Li, Kuan-Ju Chen, Nandita Niranjan, Jessica Basso, Daniel LaSala, Mei-Fong Pang, Craig Smith, Tam Nguyen, Vanessa de Carvalho Oliveira, David C. Cipolla, et al.activity in lavage fluid supernatants was quantified using the LDH-Glo Cytotoxicity Assay (Promega #J2381) according to the manufacturer’s instructions. Clarified supernatants were added to white 96-well plates,

(16) New Mechanistic Evidence for Perfluorodecanoic Acid (PFDA) Teratogenicity via CYP26A1-Mediated Retinoic Acid Metabolism and SignalingChemical Research in ToxicologyApril 20, 2026Michaela Hvizdak, Sylvie E. Kandel, Jed N. Lampeprotocols. Cytotoxicity was assessed morphologically and via the LDH-Glo cytotoxicity-assay kit (catalog no. J2380) from Promega (Madison, WI). Recombinant CYP In Vitro atRA Oxidation Protein concentration and reaction time

(17) A protocol to establish and maintain organotypic cerebellar slice culture (OCerSC) from aged micePLoS OneApril 17, 2026Michael F. Almeida, Kaitlan Smith, Michael A. Garris, Rebekah Sanchez-Hodge, Meagan Colie, Jonathan C. Schislerserving as a dependable marker of cytotoxicity [32]. We used the LDH-Glo™ Cytotoxicity Assay (Promega, J2380), a luminescent test that measures LDH activity through linked enzymatic reactions, ultimately producing

(18) Translational strategy to support the first-in-human study of a TCR-like T cell bispecific with an in vitro-based safety approachFront ImmunolApril 17, 2026Angelique Augustin, Blandine Avignon, Christophe Boetsch, Ekaterina Breous-Nystrom, Olaf Broders, Lauriane Cabon, Karen Dernick, Evelyne Durr, Miro Julian Eigenmann, Susanne Fischer, et al.release Target cell lysis was quantified via LDH release using the LDH-Glo Cytotoxicity Assay (Cat: J2380, Promega) or Caspase 3/7 cleavage using the CellEvent Caspase-3/7 Green Detection Reagent (Cat: C10423,

(19) Specific inhibition of NLRP3 inflammasome by a Smurf1 inhibitor in vitro and in vivoOpen Med (Wars)April 9, 2026Lifeng Meng, Hongmei Xuan, Ping Zhou, Weifeng Xu, Wei Bian, Yunxiao Lin, Ying GuanLDH level in the cell culture supernatants was measured by LDH Cytotoxicity Assay Kit (Promega, Cat # J2380) following the manufacturer’s instructions. Immunoblot analysis The cells were lysed with cell lysis

(20) Human Assembloid Model of Emergent Neurotropic EnterovirusesbioRxivApril 8, 2026Christine E. Peters, Jimena Andersen, Min-Yin Li, Lauren Varanese, Mayuri Vijay Thete, Se-Jin Yoon, Taylor Pio, Nicholas Thom, Xiaoyu Chen, Wenjie Qiao, et al.lactate dehydrogenase (LDH) released into the cell culture supernatant using LDH-Glo Cytotoxicity Assay (J2381; Promega). Luciferase readings were taken one hour after reagent addition and read using a GloMax 20/20 Luminometer

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LDH-Glo Cytotoxicity Assay

LDH-Glo Cytotoxicity Assay from Promega

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LDH-Glo Cytotoxicity Assay from Promega

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