Human TNF-alpha Quantikine ELISA Kit DTA00D from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman TNF-alpha Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDTA00D
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range15.60 - 1000 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity6.23 pg/mL
Detection TargetIL-19
NCBI Full Gene Nameinterleukin 19
NCBI Gene AliasesIL-10C, MDA1, NG.1, ZMDA1
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Inflammatory, oxidative, and neurotrophic profiles in monozygotic twins discordant for pain-related TMDMol Biol RepMay 14, 2026Laís Valencise Magri, Melissa Oliveira Melchior, Cecília Nogueira Tavares Peixeiro, Vitória Carolina Rondon-Pereira, Margarete Ribeiro-Dasilva, Kranya Victoria Díaz-Serrano, Riccardo Lacchini, Edilaine Cristina da Silva Gherardi-Donato, Christie Ramos Andrade Leite-Panissiwere used for IL-6 (Catalog RDSY-D6050-96SW), IL-10 (Catalog RDSY-D1000B-96SW) and TNF-α (Catalog RDSY-DTA00D-9SW). For matrix remodeling, DuoSet kits were used for MMP-2 (Catalog RDSY-DY902-1KIT), MMP-9 (Catalog

(2) Fibrinogen-like protein 2 regulates inflammatory and metabolic reprogramming of airway smooth muscle cells through PI3K/Akt activationFront Med (Lausanne)May 12, 2026Bo Zhao, Yanran Cheby centrifugation to remove cellular debris. The concentrations of tumor necrosis factor-α (TNF-α; DTA00D), interleukin-1β (IL-1β; DLB50), and interleukin-6 (IL-6; D6050B) were quantified using commercially

(3) Clinical retrospective study on serum sCD163 and CXCL10 in the immune microenvironment of carotid atherosclerosis: association with inflammatory cytokines, immune cell subsets, and disease progressionFront ImmunolMay 7, 2026Hong Pan, Huijun Zheng, Mi LiCat# DLB50): detection range 3.9–250 pg/mL; sensitivity 1.0 pg/mL. ⑤TNF-α ELISA (R&D Systems, Cat# DTA00D): detection range 15.6-1,000 pg/mL; sensitivity 5.5 pg/mL.Flow cytometry analysis of peripheral blood

(4) Fc-mediated antibody functions are associated with disease severity in COVID-19Front ImmunolMay 1, 2026Sungim Choi, Jong Su Kang, Jaehwan You, Man-Seong Park, Hee Bum Jo, Ji-Soo Kwon, Sung-Han Kim, Seong Yeon Parkfactor (TNF)-α levels were quantified using commercial ELISA kits (Koma Biotech, K0331194; R&D Systems, DTA00D) according to the manufacturer’s instructions. For IL-6 measurement, diluted plasma samples (1:3) were

(5) MiR-200a-3p protects against myocardial ischemia-reperfusion injury via KEAP1-NRF2 signalingFront PhysiolMay 1, 2026Yanbo Zhao, Lulu Liu, Xiaohua Shen, Min Wang, Meihui Wang, Lingling Sun, Kai Zhangmin to obtain serum. Cytokines were quantified using commercially available ELISA kits (human TNF-α: DTA00D; human IL-1β: DLB50; mouse TNF-α: MTA00B; mouse IL-1β: MLB00C; R&D Systems) according to the manufacturers’

(6) Adherence to the Mediterranean diet, inflammatory biomarkers and cognitive status in older Italian adultsFrontiers in NutritionApril 29, 2026Margherita Grasso, Francesca L’Episcopo, Annamaria Fidilio, Marco Antonio Olvera-Moreira, Giuseppe Toscano, Stefano Muratore, Margherita Drago, Sabrina Musso, Veronica Bentivegna, Lucrezia Costanzo, et al.concentrations of TNF-α were determined using the human TNF-α ELISA kit (Bio-Techne, R&D system, cod. DTA00D) following the protocol detailed by the manufacturer. The optical density of each well was determined

(7) Serum FGL2 is correlated with the severity and psychological status of diarrhea-predominant irritable bowel syndromeFrontiers in MedicineApril 28, 2026Fubing Wang, Xin Li, Peipei Zhao(FGL2; Cat#ml063599, Shanghai Enzyme-linked Biotechnology), tumor necrosis factor-alpha (TNF-α; Cat#DTA00D, R&D Systems), interleukin-1 beta (IL-1β; Cat#DY240, R&D Systems), interleukin-6 (IL-6; Cat#D6050B,

(8) Elevated serum sortilin reflects inflammatory burden and coagulation dysfunction in ulcerative colitisFrontiers in MedicineApril 21, 2026Jiangtao Ding, Haizhou Xualiquoted and stored at −80°C until analysis. Serum concentrations of Tumor Necrosis Factor alpha (TNF-α; DTA00D, R&D Systems), Interleukin-1 beta (IL-1β; DLB50, R&D Systems), Interleukin-6 (IL-6; D6050B, R&D Systems),

(9) Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivationPLoS OneApril 17, 2026Aiyuan Cai, Zilong Li, Ran Liu, Hailong Huang, Ping Liu, Ruizhong Zhang, Jing Xiao, Yuanhong Lin, Qingpeng Hu, Haixia Wuthe specific pull-down of the P2RY12 protein. 2.6 ELISA experiment ELISA kits (R&D Systems, USA, DTA00D for TNF-α detection; R&D Systems, USA, DLB50 for IL-1β detection) were used to detect the levels of

(10) Single nuclei/cell transcriptomics reveal DMD driven cell dynamics and mechanisms of fibroblast inflammatory tissue priming in human dystrophic muscleResearch SquareApril 15, 2026M. Carrie Chesmore, Florian Barthélémy, Deirdre Scripture-Adams, Deirdre D. Scripture-Adams, Annabel Little, Richard Nasif, Shirley Nieves-Rodriguez, Richard T. Corona, Shirley Nieves-Rodriguez, Rosario I. Corona, et al.used to measure the secretion of GZMK (Abcam, ab31436), GZMB (R&D system DY290605), TNFα (R&D system DTA00D), and IFNγ (Thermofisher ENEHIFNG5) by ELISA. Antibody labeling for feature barcode Antibodies with

(11) Astragaloside IV from Astragalus membranaceus Fisch. ex Bunge Mitigates DSS-Induced Colitis via Anti-Inflammatory and Antioxidant Modulation of the Gut–Liver–Brain AxisAntioxidants (Basel)April 10, 2026Woo-Gyun Choi, Byung Joo Kimusing commercial sandwich ELISA kits (R&D Systems, Minneapolis, MN, USA; IL-1β, Cat. DY201; TNF-α, Cat. DTA00D) according to the manufacturer’s instructions. Nitric oxide metabolite (nitrite/nitrate) levels were

(12) p65 enhanced METTL3-mediated m6A methylation of HMGB1 to promote microglia M1 polarization in sepsis-associated encephalopathyCentral European Journal of ImmunologyMarch 31, 2026Xiaowen Wu, Jie Wang, Jun Li, Guosheng Yao, Linyun Wei, Xuebin LiEnzyme-linked immunosorbent assay (ELISA) Concentrations of inflammatory cytokines, such as tumor necrosis factor α (TNF-α, #DTA00D), interleukin 1β (IL-1β, #DLB50), and IL-6 (#D6050), were assessed using ELISA kits from R&D Systems

(13) A machine learning model integrating gut microbiota biomarkers for predicting neurological recovery after cerebral hypoxia-ischemia: a single-center study augmented with public dataFrontiers in PharmacologyMarch 31, 2026Ling Kong, Chunhua Wangquantified using commercial enzyme-linked immunosorbent assay (ELISA) kits (Human TNF-α ELISA Kit, Cat. No. DTA00D; Human IL-6 ELISA Kit, Cat. No. D6050; Human IFN-γ ELISA Kit, Cat. No. DIF50C; R&D Systems, Minneapolis,

(14) Anti-HPV gel versus interferon α-2b gel as adjuvant therapy after LEEP for HSIL with HR-HPV infection on clinical outcomes, vaginal microecology, and biomarkers of proliferation and immunityFrontiers in OncologyMarch 25, 2026Xu Long, Mengjun Luo, Chunrong Yang, Fengying Yan, Hongou Pu, Xiaohong Lienzyme-linked immunosorbent assay (ELISA) kits (e.g., IL-17: Cat# D1700, R&D Systems, Minneapolis, MN, USA; TNF-α: Cat# DTA00D, R&D Systems; IFN-γ: Cat# DIF50, R&D Systems; TGF-β: Cat# DB100B, R&D Systems; Survivin: Cat# ab119892,

(15) Deficiency of interleukin-40 prevents intestinal damage in experimental necrotizing enterocolitis by inhibiting NETosisCell Communication and SignalingMarch 7, 2026Yunfei Zhang, Cuilian Ye, Xin Zhong, Xionghui Ding, Yihang Yang, Yue Ma, Chunbao GuoName,Source,Identifier Human IL-1β,R&D systems,Catalog #: DLB50 Human TNF-α,R&D systems,Catalog #: DTA00D Human IL-6,R&D systems,Catalog #: QK206 Human Procalcitonin,R&D systems,Catalog #: DY8350-05 Human

(16) Vascular STING activation facilitates NK cell anti-tumor immunity in small cell lung cancerCancer CellMarch 5, 2026Marco Campisi, Tatsuya Osaki, Ian Dryg, Carla Stornante, Jacqueline Wolff, Jason Weirather, Nicholas Weaver, Mubin Tarannum, Ian Gillanders, Alan Bers, et al.collected after 72 hours. Human CXCL10 ELISA (R&D Systems, Cat# SIP100), human TNFα (R&D Systems, Cat# DTA00D) and human IFNβ (R&D Systems, Cat# DIFNB0) were performed according to manufacturer’s instructions.

(17) Upregulation of IL-32γ in endothelial cells promotes macrophage recruitment and inflammatory progression in atherosclerosisScientific ReportsFebruary 27, 2026Zicong Yang, Mengzhen Liu, Yin Shi, Zhengde Lu, Zhao Lu, Yingzhong Lin, Qingwei Ji, Ling LiuCo., Ltd., MO-P31364R). IL-1β and TNF-α were measured using ELISA kits from R&D Systems (DLB50 and DTA00D). All assays were performed according to manufacturers’ instructions. Cell proliferation assay Cell

(18) Epigenetic reactivation of the tumor suppressor ZBTB7A by KDM4 inhibition in human acute myeloid leukemiaScience Translational MedicineFebruary 25, 2026Alexander Arnuk, Cuijuan Han, Abimbola E. Lawal, Bofei Wang, Sadik Karma, Zhiping Zhang, Mhd Yousuf Yassouf, Sakthi Harini Rajendran, Harshpreet Chandok, Madeline L. Eller, et al.hematopoietic lineages in bone marrow and peripheral blood. ELISA assays Concentrations of human TNF-α (Cat. #DTA00D), IL-1β (Cat. #DLB50), MMP-9 (Cat. #DMP900), and MPO (Cat. #DMYE00B) in cell culture media were quantified

(19) Telomere shortening correlates with immune dysregulation and severity in pulmonary tuberculosisBMC Pulmonary MedicineFebruary 25, 2026Fanny Fachrucha, Sita Andarini, Erlina Burhan, Heri Wibowo, Aria Kekalih, Moulid Hidayat, Galoeh Adyasiwi, Farhana Ibrahim Syuaib, Wiwien Heru Wiyonoinitiation of ATT. Quantification was performed with R&D Systems Quantikine ELISA kits (TNF-α, catalog no. DTA00D; IL-10, catalog no. D1000B) according to the manufacturer’s instructions. Telomere length measurement Leukocyte

(20) LncRNA RNF144A-AS1 increases P300-mediated H4K5 acetylation of PDE4 to promote keloid fibrosisBioMedical Engineering OnLineFebruary 14, 2026Yujing Liu, Suihai Wang, Shanshan Wei, Xianwen Qiu, Yijie Mei, Lu Yantumor necrosis factor-α (TNF-α), and interleukin (IL)-1β levels were measured using ELISA kits (DLAP00/DTA00D/QK201, Bio-techne, Minneapolis, MN, USA) according to the manufacturer’s instructions. Absorbance at

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(1)

5/17/2022Kit Didn't Work, but Likely User Error Stephanie BaringerTried to use for media from astrocytes activated by amyloid beta. I did not detect any TNF-a, however, I discovered after the fact that astrocytes often do not secrete much TNF-a in response to ... Read Review
(1)

8/16/2021Most Accurate Human TNF-alpha Dr. Wei XuTo detect human TNF-a production, we tried many different kits. This kit give great reading and accuracy in TNF-a level from cell supernatant.

TNF-alpha ELISA