Fig 1: Acute and chronic Daxx loss lead to perturbations of haematopoiesis.a, Total number of cells (n = 5 WT and 7 Daxx-KO mice). b, Frequency and total cell number of stem, multipotent and progenitor populations (n = 4 WT and 5 Daxx-KO mice). c, Frequency and total cell number of mature lymphoid and myeloid cells(n = 6 WT and 7 Daxx-KO mice). d, Frequency and total cell numbers of B cell-progenitor populations (n = 4 WT and 5 Daxx-KO mice). e, Frequency and total counts of mature lymphoid and myeloid cells (n = 4 mice per genotype). f, Frequencies of apoptotic B cells (n = 4 WT and 5 Daxx-KO mice). g, Frequency and total cell numbers of B cells and neutrophils at 2 w.p.i. (n = 3 mice per genotype). h, Representative images of haematoxilin and eosin (H&E) staining of bones (n = 2 independent experiments). Scale bars, 100 µm (left) and 20 µm (right; higher-magnification images). i, Frequencies of immature and mature neutrophils (Gr1+CD11b+ cells; n = 3 mice per genotype). j, Examples of the flow cytometry analysis of myeloid progenitor cells. k,l, Frequency (k) and total count (l) of haematopoietic progenitors (n = 3 mice per genotype). m, Concentration of different cytokines in plasma (n = 6 mice per genotype; analysis of variance and corresponding non-parametric Conover’s test). TPO, thrombopoietin. n, Frequencies of neutrophils (n = 6 mice per genotype; non-parametric Mann–Whitney test). o, Number of neutrophils (n = 5 mice per genotype; non-parametric Mann–Whitney test). p, Cxcr2 levels measured in mature neutrophils (n = 3 mice per genotype). MFU, mean fluorescent units. q, Examples of flow cytometry analysis of B cell progenitors and frequencies of B cells and progenitors (right; n = 6 mice per genotype; non-parametric Mann–Whitney test). a–g, RosaCreERT2 mice. h–q, Mx1Cre mice. a–d,i–l,n–q, BM. e–g, Spleen. a–g,i,k,l,p, Student’s t-test. b–g,i,k–o, Boxplots show the minimum and maximum values (box boundaries) and the mean (horizontal line). a,p, Data are the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 and NS, not significant. Daxx F/F, Daxx KO and Daxx +/+, Daxx WT. Exact P values and numerical source data are provided.Source data