LDH-Cytox™ Assay Kit 426401 from BioLegend

Product Specs
ItemLDH-Cytox™ Assay Kit
CompanyBioLegend
Price $229.00Supplier Page View Company Product Page
Catalog Number426401
Quantity500 tests
ApplicationsCyt - Quality tested, FA, BA - Reported in the literature, not verified in house
FormatThis solution contains WST

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Lipid droplet-induced T cell death sustains autoimmune tissue inflammationCell MetabolismApril 7, 2026Jitendra Kumar, Yoshinori Takashima, Jose Morales, Brandon Wong, Lina Werner, Isabel N. Goronzy, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jörg J. Goronzy, et al.EasySep™ Human T Cell Isolation Kit,STEMCELL,#100-0695 ELISA MAX™ Deluxe Set Human IL-1β,BioLegend,# 437004 LDH-Cytox™ Assay Kit,BioLegend,#426401 Lipid Droplet Isolation Kit,"Cell Biolabs, Inc.",# MET-5011 MojoSort™ Human CD4 T Cell Isolation Kit,BioLegend,#480130 NE-PER™

(2) Tracking tau and cellular responses in human iPSC-microglia: from uptake to seedable secretion, including in extracellular vesiclesAlzheimers DementApril 6, 2026Maria Kreger Karabova, Anna Del Ser-Badia, Anne Hedegaard, Sam J. Washer, Zeynep Baykam, Darragh P. O'Brien, Iolanda Vendrell, Svenja S. Hester, Roman Fischer, Errin Johnson, et al.brain tau seed preparation was assessed using a lactate dehydrogenase (LDH) detection kit (Catalog No. 426401, Biolegend), following the manual for “Assay using cell supernatant.” In short, macrophage precursors

(3) Stem cell-derived brain-like endothelial cells to interrogate Streptococcus pneumoniae interaction with brain endotheliumVirulenceOctober 3, 2025Henry D. Mauser, Taryn E. Keyzer, Jessica M. Surma, Natalie G. Alexander, William D. Cutts, Sarah F. Hathcock, Kimberly H. Lackey, Daryl W. Lam, Justin A. Thornton, Nadine Vollmuth, et al.cytotoxicity of pneumococcus toward iBECs was measured using the LDH-Cytox™ Assay Kit (Biolegend cat#426401). iBECs in a standard 96-well plate were infected as previously described with 62.5 μL of EC -/- containing

(4) A novel 2-oxopyrrolidine derivative (LN-53) efficiently induces Nrf-2 signaling pathway activation in human epidermal keratinocytesPharmacological reports : PROctober 1, 2025Sarac BE, Nissim L, Karaguzel D, Arik G, Kahremany S, Korshin EE, Gruzman A, Karaaslan C.(LDH) activity measurement Cytotoxic effects of LN-53 were assessed by LDH-Cytox™ Assay Kit (cat. no. 426401) (Biolegend, USA) as previously described [29]. Lysis buffer containing Triton X-100 supplied by the

(5) Red Cabbage Anthocyanin-Loaded Bacterial Cellulose Hydrogel for Colorimetric Detection of Microbial Contamination and Skin Healing ApplicationsPolymers (Basel)July 31, 2025Hanna Melnyk, Olesia Havryliuk, Iryna Zaets, Tetyana Sergeyeva, Ganna Zubova, Valeriia Korovina, Maria Scherbyna, Lilia Savinska, Lyudmila Khirunenko, Evzen Amler, et al.in a 5% CO2 at 37 °C. LDH activity was measured in the supernatant using the LDH CytoxTM Assay Kit (426401; BioLegend, San Diego, CA, USA) according to the manufacturer’s protocol and read at 490 nm on a BioRad

(6) Caspase-11 drives macrophage hyperinflammation in models of Polg-related mitochondrial diseaseNat CommunMay 20, 2025Jordyn J. VanPortfliet, Yuanjiu Lei, Muthumeena Ramanathan, Camila Guerra Martinez, Jessica Wong, Tim J. Stodola, Brian R. Hoffmann, Kathryn Pflug, Raquel Sitcheran, Stephen C. Kneeland, et al.measured in the supernatant of PAO1 challenged macrophages using a LDH-Cytox Assay Kit (Biolegend, 426401) according to manufacturer’s recommendations. A set of cells was treated with 1% Triton X-100, and the

(7) CDH17-targeting CAR-NK cells synergize with CD47 blockade for potent suppression of gastrointestinal cancersActa Pharmaceutica Sinica BMay 1, 2025Liuhai Zheng, Youbing Ding, Xiaolong Xu, Huifang Wang, Guangwei Shi, Yang Li, Yuanqiao He, Yue Gong, Xiaodong Zhang, Jinxi Wei, et al.folic acid (#F8758-5G) were purchased from Sigma–Aldrich (St. Louis, MO, USA). LDH-Cytox Assay Kit (#426401) was purchased from BioLegend (San Diego, CA, USA). Lenti-X Concentrator (#631231) was purchased from

(8) Transcriptomic analysis of coxsackievirus B3 infection in induced pluripotent stem cell-derived brain-like endothelial cellsJournal of VirologyJanuary 31, 2025Sarah F. Hathcock, Julia Mamana, Taryn E. Keyzer, Nadine Vollmuth, Mohammad-Reza Shokri, Henry D. Mauser, Robert N. Correll, Daryl W. Lam, Brandon J. Kim, Jon Sinwas measured using the LDH-Cytox Assay Kit according to the manufacturer’s instructions (Biolegend; 426401). iBECs were grown in a 24-well plate and infected as previously described at an MOI of 10 for 2 or

(9) Sulfated glycosaminoglycans are host epithelial cell targets of the Candida albicans toxin candidalysinNature microbiologyOctober 1, 2024Lin J, Miao J, Schaefer KG, Russell CM, Pyron RJ, Zhang F, Phan QT, Solis NV, Liu H, Tashiro M, et al.repeated three times, with 2–3 replicates each. In some experiments, an LDH-Cytox assay (Biolegend, #426401) was used to measure epithelial cell damage. In these experiments, the epithelial cells were grown in

(10) Hypersensitivity to type I interferon as a cause of hydrocephalus developmentBrain ResearchOctober 1, 2024Kei-ichiro Arimoto, Yue Zhang, Shinobu Matsuura, Sayuri Miyauchi, Dong-Er Zhangdehydrogenase (LDH) released from cells into culture supernatants was measured using the LDH Cytox™ Assay Kit (426401; BioLegend) according to the manufacturer’s protocol. 2.9. Histology Whole brain was fixed by 10%

(11) A nonadjuvanted HLA-restricted peptide vaccine induced both T and B cell immunity against SARS-CoV-2 spike proteinScientific ReportsSeptember 4, 2024Yi-Ru Lee, Chiung-Wen Liou, I-Hua Liu, Jia-Ming Changfor 5 days at 37 °C and 5% CO2. Cytotoxicity was measured by using an LDH-Cytox™ assay kit (Cat No. 426401, BioLegend, CA, USA). SARS-CoV-2 spike-expressing cells without PBMCs and peptide treatment were used

(12) Combination of compound screening with an animal model identifies pentamidine to prevent Chlamydia trachomatis infectionCell Reports MedicineJuly 16, 2024Katja Knapp, Romana Klasinc, Anna Koren, Magdalena Siller, Ruth Dingelmaier-Hovorka, Mathias Drach, Juan Sanchez, David Chromy, Marlene Kranawetter, Christoph Grimm, et al.Violet,Thermo Fisher Scientific,Cat# C34557 7-AAD,BD,Cat# 559925 LDH-Cytox™ Assay Kit,Biolegend,Cat# 426401 QIAamp DNA mini kit,Qiagen,Cat# 51306 Phalloidin AF594,Invitrogen,Cat# A12381 Luna® Universal Probe qPCR Master Mix

(13) A novel model to study mechanisms of cholestasis in human cholangiocytes reveals a role for the SIPR2 pathwayHepatology CommunicationsMarch 1, 2024Diana Islam, Izza Israr, Mohamed A. B. Taleb, Aditya Rao, Robel Yosief, Rukhsar Sultana, Fotios Sampaziotis, Olivia C. Tysoe, Michael Trauner, Saul J. Karpen, et al.dehydrogenase. Cytotoxicity Lactate dehydrogenase (LDH) release is a hallmark of cell death. An LDH-Cytox Assay Kit (426401, BioLegend) was used to access cytotoxicity by measuring LDH activity in cell culture media following

(14) Determination of plastic polyester oligomers in real samples and their bioeffectsEFSA JournalDecember 4, 2023Sara Di Lonardo, Dimitra Diamantidou, Aikaterini‐Theodora Chatzitaki, Dimitris Fatouros, Georgios Theodoridisanalysis by flow cytometry. Cytotoxicity was tested by lactate dehydrogenase assay (Biolegend, Cat# 426401). The determination of cytotoxicity has been performed by measuring lactate dehydrogenase activity released

(15) Expansion of interferon inducible gene pool via USP18 inhibition promotes cancer cell pyroptosisNature communicationsJanuary 17, 2023Arimoto KI, Miyauchi S, Troutman TD, Zhang Y, Liu M, Stoner SA, Davis AG, Fan JB, Huang YJ, Yan M, et al.dehydrogenase (LDH) released from cells into culture supernatants was measured using the LDH CytoxTM Assay Kit (426401; BioLegend) according to the manufacturer’s protocol. The percentage of LDH release (Cytotoxicity) was

(16) Antigens Expressed by Breast Cancer Cells Undergoing EMT Stimulate Cytotoxic CD8(+) T Cell ImmunityCancersSeptember 9, 2022Camp FA, Brunetti TM, Williams MM, Christenson JL, Sreekanth V, Costello JC, Hay ZLZ, Kedl RM, Richer JK, Slansky JE.either 3 days for flow cytometry proliferation assays or 24 h for LDH cytotoxic assays (Biolegend Cat. #426401) and IFNγ ELISA (ThermoFisher, Cat. #88-7314-22) following manufacture’s protocol. 3. Results 3.1.

(17) Interferon-γ Preferentially Promotes Necroptosis of Lung Epithelial Cells by Upregulating MLKLCellsFebruary 6, 2022Hao Q, Shetty S, Tucker TA, Idell S, Tang H.dehydrogenase (LDH) activities in cell supernatants were determined by using the LDH-Cytox™ assay kit (no. 426401, Biolegend, San Diego, CA, USA) according to the manufacturer’s protocol. Relative changes in the release

(18) Characterization of the COPD alveolar niche using single-cell RNA sequencingNature communicationsJanuary 25, 2022Sauler M, McDonough JE, Adams TS, Kothapalli N, Barnthaler T, Werder RB, Schupp JC, Nouws J, Robertson MJ, Coarfa C, et al.microplate reader). Cytotoxicity was assayed by using a lactate dehydrogenase (LDH)-cytox kit (Biolegend #426401). In brief, cells were seeded into 96-well plates and incubated with the indicated treatments. 50 μL

(19) A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic strokeNature biomedical engineeringAugust 1, 2021Lyu Z, Park J, Kim KM, Jin HJ, Wu H, Rajadas J, Kim DH, Steinberg GK, Lee W.Viability kit (ThermoFisher Scientific, Cat. No.: L3224) and LDH-Cytox Assay™ kit (BioLegend, Cat. No.: 426401), respectively. Cell viability was calculated as the number of viable cells divided by the total number

(20) MSCs loaded with oncolytic reovirus: migration and in vivo virus delivery potential for evaluating anti-cancer effect in tumor-bearing C57BL/6 miceCancer cell internationalMay 1, 2021Seyed-Khorrami SM, Soleimanjahi H, Soudi S, Habibian A.Other steps were performed according to the manufacturer’s instruction of LDH kit (BioLegend, Cat. No: 426401). Finally, all wells absorbance was measured at 490 nm. Cytokine assay The enzyme-linked immunosorbent assay

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4/17/2023Sensitive LDH Assay from BioLegend Soheil TavakolpourLDH-Cytox Assay™ Kit is a kit for determination of cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells. LDH catalyzes dehydrogenation of lactate to pyruvate thereby ... Read Review

LDH-Cytox™ Assay Kit from BioLegend

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LDH-Cytox™ Assay Kit from BioLegend

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