Mitochondrial Superoxide Assay Kit (Fluorometric) ab219943 from Abcam

Product Specs
ItemMitochondrial Superoxide Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab219943
Quantity200 Test
Sample TypeAdherent cells, Suspension cells

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(1) Multiomic Analysis Reveals Molecular Pathways Associated with Intestinal Aggregation of α-SynucleinACS Chemical BiologyJanuary 16, 2026Julia M. Balsamo, Ying Yan, Dylan Thai, Stephanie M. Cologna, Elizabeth N. Bessinvestigated as a function of superoxide generation using a MitoSOX fluorescence-based assay kit (Abcam, ab219943). STC-1 cells were seeded onto a 96-well clear-bottom black-wall plate (Greiner) at a density of 6.5

(2) Astragalus membranaceus injection activates mitophagy and protects mitochondrial function in chronic heart failure via inhibiting AKT/mTOR pathwayScientific ReportsNovember 28, 2025Sinai Li, Juju Shang, Wenlong Xing, Mingxue Zhou, Shenglei Qiu, Weihong Liu, Lei Zhang, Haoyue Shi, Huanning Niu, Songtao Kuang, et al.manufacturer’s protocol. Mitochondrial ROS were detected using mitochondrial superoxide assay kit (ab219943, Abcam) according to the manufacturer’s protocols. Assessment of mitochondrial morphology and mitophagy Cardiomyocytes

(3) Detrimental Synergistic Effects of Atmospheric Ozone and Polystyrene Nanoparticle Exposure on Human Adult Myogenic Progenitor CellsBiochemistry Research InternationalNovember 14, 2025Cristina Purcaro, Ester Sara Di Filippo, Cecilia Paolini, Piero Di Carlo, Stefania Fullesuperoxide levels were assessed through the Mitochondrial Superoxide Detection Kit (Fluorometric, #ab219943, Abcam, UK), according to the manufacturer's instructions. huMPCs were seeded in a 96-well black-bottom

(4) The MEK inhibitor trametinib incurs mitochondrial injury and induces innate immune responses in the mouse heartbioRxivSeptember 16, 2025Kelsey H. Fisher-Wellman, Richard D. Lutze, Logan G. Kirkland, Ju Youn Beak, Samantha M. Morrissey, Peyton B. Sandroni, Wei Huang, Julian D. Bailon, Melissa A. Schroder, Lars A. Albrecht, et al.representative of MEKi’s, we measured superoxide production using a Mitochondrial Superoxide Assay Kit (Abcam, ab219943) on NRVMs exposed to Trm, cobimetinib or selumetinib. All three MEK inhibitors induced a roughly 50%

(5) Multiomic Analysis Reveals Molecular Pathways Associated with Intestinal Aggregation of α-SynucleinbioRxivSeptember 11, 2025Julia M. Balsamo, Ying Yan, Dylan Thai, Stephanie M. Cologna, Elizabeth N. Bessinvestigated as a function of superoxide generation using a MitoSOX fluorescence-based assay kit (Abcam, ab219943). STC-1 cells were seeded onto a 96-well clear-bottom black-wall plate (Greiner) at a density of 6.5

(6) Investigating the Potential of Propranolol as an Anti-Tumor Agent in Colorectal Cancer Cell LinesInt J Mol SciAugust 4, 2025Shiekhah Mohammad Alzahrani, Huda Abdulaziz Al Doghaither, Hind Ali Alkhatabi, Mohammad Abdullah Basabrain, Peter Natesan Pushparajapoptosis was mediated by mitochondria via excess formation of Mito ROS using the MitoROS 580 kit (ab219943, Abcam, Cambridge, UK) [44]. Briefly, 1 × 104 cells/well of CRC cell lines were seeded in 96-well plates

(7) Propranolol and Capecitabine Synergy on Inducing Ferroptosis in Human Colorectal Cancer Cells: Potential Implications in Cancer TherapyCancers (Basel)April 27, 2025Shiekhah Mohammad Alzahrani, Huda Abdulaziz Al Doghaither, Hind Ali Alkhatabi, Mohammad Abdullah Basabrain, Peter Natesan PushparajMitoROS assay was performed according to the manufacturer’s instructions using the MitoROS 580 kit (ab219943, Abcam, Cambridge, UK). Red fluorescence intensity was measured using a SpectraMax i3 fluorometric microplate

(8) Protective Action of 3,5-Diiodo-L-Thyronine on Cigarette Smoke-Induced Mitochondrial Dysfunction in Human Alveolar Epithelial CellsBiomedicinesApril 22, 2025Francesca Panico, Davida Mirra, Giuseppe Petito, Giuseppe Spaziano, Vitale Del Vecchio, Renata Esposito, Rosalba Senese, Vincenzo Desiderio, Antonia Lanni, Bruno D’AgostinoA549 cells, superoxide anion species were measured with the Mitochondrial Superoxide Detection Kit (ab219943, Abcam, Cambridge, UK), as described by Yang et al. [35] and following the instructions of the manufacturer.

(9) Mitochondrial hyperactivity and reactive oxygen species drive innate immunity to the yellow fever virus-17D live-attenuated vaccinePLOS PathogensApril 21, 2025Samantha G. Muccilli, Benjamin Schwarz, Byron Shue, Forrest Jessop, Jeffrey G. Shannon, Charles L. Larson, Adam Hage, Seon-Hui Hong, Eric Bohrnsen, Thomas Hsu, et al.poly-L-lysine. ROS production was measured at 48 hpi with the Mitochondrial Superoxide Detection Kit (Abcam, ab219943). Cells were loaded for 30 min with MitoROS 580 and 0.1 ug/ml Hoechst 33342 (Thermo) in growth medium

(10) Acidic Nanoparticles Restore Lysosomal Acidification and Rescue Metabolic Dysfunction in Pancreatic β-Cells under Lipotoxic ConditionsACS NanoJune 18, 2024Chih Hung Lo, Lance M. O’Connor, Gavin Wen Zhao Loi, Eka Norfaishanty Saipuljumri, Jonathan Indajang, Kaitlynn M. Lopes, Orian S. Shirihai, Mark W. Grinstaff, Jialiu Zengmitochondrial superoxide release was measured with the Mitochondrial Superoxide Assay Kit (Abcam, Cat# ab219943), according to the manufacturer’s instructions. Measurements were acquired using a Synergy H1 plate

(11) Ohwia caudata aqueous extract attenuates senescence in aging adipose-derived mesenchymal stem cellsHeliyonMay 15, 2024Tsung-Jung Ho, Bruce Chi-Kang Tsai, Goswami Debakshee, Marthandam Asokan Shibu, Chia-Hua Kuo, Chih-Hsueh Lin, Pi-Yu Lin, Shinn-Zong Lin, Wei-Wen Kuo, Chih-Yang Huang× 100 %. 2.6 Mitochondrial superoxide assay A mitochondrial superoxide assay kit (fluorometric) (ab219943, Abcam) was used to measure mitochondrial superoxide levels in hADMSCs. Following 24 h of doxorubicin

(12) TREM1 disrupts myeloid bioenergetics and cognitive function in aging and Alzheimer disease mouse modelsNature neuroscienceMay 1, 2024Wilson EN, Wang C, Swarovski MS, Zera KA, Ennerfelt HE, Wang Q, Chaney A, Gauba E, Ramos Benitez JA, Le Guen Y, et al.37°C and 5% CO2. Media was replaced the following day. Mitochondrial Superoxide Assay Kit (Abcam, Cat# ab219943) and MitoTracker Deep Red FM (Invitrogen, M22426) were used to measure mitochondrial superoxide production

(13) Thyroid hormone protects human lung epithelial cells from cold preservation and warm reperfusion-induced injuryJ Transl MedMarch 1, 2024Dejan Bojic, Tanroop Aujla, Junichi Sugihara, Aaron Wong, Shaf Keshavjee, Mingyao LiuIR was simulated as described above. Mitochondrial Superoxide Detection Kit (Fluorometric) (Abcam, ab219943) reagents were thawed at room temperature and protected from light in preparation. MitoROS 580 Stain

(14) The alpha-1A adrenergic receptor regulates mitochondrial oxidative metabolism in the mouse heartJournal of Molecular and Cellular CardiologyFebruary 1, 2024Peyton B. Sandroni, Melissa A. Schroder, Hunter T. Hawkins, Julian D. Bailon, Wei Huang, James T. Hagen, McLane Montgomery, Seok J. Hong, Andrew L. Chin, Jiandong Zhang, et al.2-minute intervals over a 60 minute time period using the Mitochondrial Superoxide Assay Kit (Abcam, ab219943) with MitoROS 580 dye using a plate reader (BMG Labtech, CLARIOstar). Fluorescence (Ex/Em 540/590) was

(15) Electron Leak From the Mitochondrial Electron Transport Chain Complex I at Site I(Q) Is Crucial for Oxygen Sensing in Rabbit and Human Ductus ArteriosusJournal of the American Heart AssociationJuly 4, 2023Read AD, Bentley RET, Martin AY, Mewburn JD, Alizadeh E, Wu D, Lima PDA, Dunham-Snary KJ, Thébaud B, Sharp W, et al.cells were loaded with 10 μmol/L of MitoROS 580 dye using the Mitochondrial Superoxide Detection Kit (ab219943; Abcam). MitoROS was incubated with the cells for 30 minutes at 37 °C in a hypoxic incubator, in the

(16) Role of Cannabidiol and Tetrahydrocannabivarin on Paclitaxel-induced neuropathic pain in rodentsInternational immunopharmacologyJune 1, 2022Kumar Kalvala A, Bagde A, Arthur P, Kumar Surapaneni S, Ramesh N, Nathani A, Singh M.Cat# 17502048), NGF (Sigma Aldrich, MO, USA, Cat# N8133), MITOSOX Stain (Abcam, Boston, MA, USA, Cat# ab219943), Neurite outgrowth assay kit (Thermo Fischer scientific, Waltham, MA, USA, Cat# A15001), TPER (Thermo Fischer scientific

(17) AXL Promotes Metformin-Induced Apoptosis Through Mediation of Autophagy by Activating ROS-AMPK-ULK1 Signaling in Human Esophageal AdenocarcinomaFrontiers in oncologyJanuary 1, 2022Hong J, Maacha S, Pidkovka N, Bates A, Salaria SN, Washington MK, Belkhiri A.Oxygen Species Detection Assay (ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator kits (ab219943) were purchased from ABCAM (Cambridge, United Kingdom). Metformin (NDC 50228-105-01) was obtained from

(18) Effect of Estrogen on Heteronemin-Induced Anti-proliferative Effect in Breast Cancer Cells With Different Estrogen Receptor StatusFrontiers in Cell and Developmental BiologyJuly 26, 2021Yu-Chen S. H. Yang et al.fluorescence-based assay. Mitochondrial ROS was detected with a Mitochondrial Superoxide Detection Kit (ab219943, Abcam, Cambridge, United Kingdom). MCF-7 cells were cultured as previously described. After being starved

(19) The anti-malarial drug atovaquone potentiates platinum-mediated cancer cell death by increasing oxidative stressCell Death DiscoveryDecember 1, 2020James T. T. Coates, Gonzalo Rodriguez-Berriguete, Rathi Puliyadi, Thomas Ashton, Remko Prevo, Archie Wing, Giovanna Granata, Giacomo Pirovano, Gillies W. McKenna, Geoff S. HigginsThe viability of controls was set to 100%. Live fluorescent mitochondrial ROS indicators MitoSOX (ab219943, Abcam) and MitoPY1 (SML0734, Sigma) were stored at −80 °C and dissolved in DMSO to stock concentrations

(20) Dietary magnesium supplementation improves lifespan in a mouse model of progeriaEMBO Molecular MedicineOctober 7, 2020Ricardo Villa‐Bellostaproduction of mitochondrial superoxide radical in live cells, the Mitochondrial Superoxide Detection Kit (ab219943, Abcam) was used, according to the manufacturer's protocols. Superoxide was measured using fluorescence

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Mitochondrial Superoxide Assay Kit (Fluorometric) from Abcam

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Mitochondrial Superoxide Assay Kit (Fluorometric) from Abcam

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