Caspase-1 (active) Staining Kit - Green Fluorescence ab219935 from Abcam

Product Specs
ItemCaspase-1 (active) Staining Kit - Green Fluorescence
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab219935
Quantity25 Test
Sample TypeAdherent cells, Suspension cells
TypeStain
ApplicationsEnzyme activity assay
Gene NameCASP1
NCBI Gene AliasesICE, IL1BC, P45
Research AreaImmunology & Infectious Disease
NCBI Full Gene Namecaspase 1

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.populations. Cells were further stained for caspase 1 cleavage with Caspase-1 (active) Staining Kit (Abcam, ab219935), or fixed for caspase 3 analysis using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate)

(2) USP7 overexpression prevents the progression of clear cell renal cell carcinoma by enhancing pyroptosis via TRIP12 deubiquitinationCancer Biology & TherapyDecember 31, 2025Hongsheng Li, Yao Ning, Junjie Yu, Yiju Chen, Qiang He, Juan Jinwashing with PBS, cells were suspended in 300 µL of binding buffer, and stained with caspase-1 staining (#ab219935, Abcam) and PI for 1 hat 25°C in dark. Subsequently, cells were measured by flow cytometry on a CytoFLEX S flow cytometer

(3) Baicalein Reduces Pyroptosis of Acinar Cells in Hyperlipidemic Acute Pancreatitis by Inhibiting M1 Polarization of Macrophages via the HMGB1/TLR4/NLRP3 PathwayInflammationMarch 5, 2025Xiangyang Wang, YaXiong Zhou, Yilei Liu, Tingting Mo, Zhiyuan Chen, Yu Zhang, Li Yang, Peng Liu(A00-1–1102, Beckman, USA) was used to detect macrophage polarization. A Pyroptosis/Caspase-1 Assay Green Kit (ab219935, Abcam, UK) was used to evaluate pyroptosis via flow cytometry according to the manufacturer’s instructions.

(4) Curcumin inhibits the activity of ubiquitin ligase Smurf2 to promote NLRP3‑dependent pyroptosis in non‑small cell lung cancer cellsInt J OncolFebruary 13, 2025Yunzhu Xi, Saili Zeng, Xiaowu Tan, Xiaoyu Dengand washed thoroughly. The cells were subsequently treated with caspase-1 working solution (cat. no. ab219935; Abcam) for 5 min at 22°C, followed by staining with PI for 30 min at 22°C. The mixture was subsequently

(5) T follicular helper cell is essential for M2 macrophage polarization and pulmonary vascular remodeling in hypoxia-induced pulmonary hypertensionRespiratory researchDecember 4, 2024Li C, Liu P, Zhu H, Yang H, Zha J, Yao H, Zhang S, Huang J, Li G, Jiang G, et al.using IF staining. Fizz1 (1:500, ab39626, Abcam), α-SMA (1:200, ab124964, Abcam) and caspase-1 (1:500, ab219935, Abcam) were assayed by ICC staining. Methods for IF and ICC staining were described in our previous

(6) Simvastatin induces pyroptosis via ROS/caspase-1/GSDMD pathway in colon cancerCell Communication and SignalingNovember 16, 2023Wei Xie, Mingjing Peng, Ying Liu, Bocheng Zhang, Liang Yi, Ying Longpurchased from ProteinTech Co. Ltd (Illinois, USA). For flow cytometry, active caspase-1 staining kit (ab219935) was purchased from Abcam Inc. (Cambridge, UK). Cell lines HCT116 cell line was a gift from Dr. Longzheng

(7) hASCs-derived exosomal miR-155-5p targeting TGFβR2 promotes autophagy and reduces pyroptosis to alleviate intervertebral disc degenerationJournal of orthopaedic translationMarch 1, 2023Chen D, Jiang X, Zou H.Cell pyroptosis The caspase-1+PI expression was detected using the caspase-1 pyroptosis assay kit (AB219935, Abcam). First, the dye solution was prepared, and 300 μL Washing buffer was added with 2 μL FAM-YVAD-FMK

(8) Damage-induced pyroptosis drives endogenous thymic regeneration via induction of Foxn1 by purinergic receptor activationbioRxivJanuary 25, 2023Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Antonella Cardinale, Lorenzo Iovino, Paul deRoos, Kayla S. Hopwo, Colton W. Smith, David Granadier, Lucas B. Sullivan, et al.populations. Cells were further stained for caspase 1 cleavage with Caspase-1 (active) Staining Kit (Abcam, ab219935), or fixed for caspase 3 analysis using Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate)

(9) Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytesFrontiers in ChemistryAugust 10, 2022Xiao Wang et al.assay Caspase-1 fluorescence staining was done using the Caspase-1 (active) Staining Kit-Green Fluorescence (#ab219935, Abcam Ltd., Waltham, MA). HaCaT cells were treated with various concentrations of complanatuside in

(10) Keratinocyte death by ferroptosis initiates skin inflammation after UVB exposureRedox BiologyNovember 1, 2021Kavita Vats, Oleg Kruglov, Alicia Mizes, Svetlana N. Samovich, Andrew A. Amoscato, Vladimir A. Tyurin, Yulia Y. Tyurina, Valerian E. Kagan, Yuri L. Bunimovichdetached with trypsin, and incubated in PBS with FAM-YVAD-FMK (Active Caspase-1 Staining Kit, Abcam, ab219935, 1:150), C11-BODIPY 581/591 (Invitrogen, D3861, 10 μM) or Red-DEVD-FMK (CaspGLOW Red Active Caspase-3 Staining Kit

(11) Inflammasome-Independent Leukotriene B(4) Production Drives Crystalline Silica-Induced Sterile InflammationJournal of immunology (Baltimore, Md. : 1950)May 15, 2018Hegde B, Bodduluri SR, Satpathy SR, Alghsham RS, Jala VR, Uriarte SM, Chung DH, Lawrenz MB, Haribabu B.confocal microscopy. For time-lapse studies, LPS-primed BMDMs were loaded with 1 mM BODIPY or active caspase-1 stain (ab219935; Abcam) along with nuclear stain Hoechst. After an hour, cells were stimulated with 35 mg/cm2 CS for different

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Caspase-1 (active) Staining Kit - Green Fluorescence from Abcam

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Caspase-1 (active) Staining Kit - Green Fluorescence from Abcam

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