Fig 1: BIGH3 levels in supernatant of human macrophages treated with LPS and TGFβ (A) and human pancreatic fibroblasts treated with TGFβ (B). CD14 + monocytes were cultured in the presence of M-CSF alone, M-CSF + TGFβ or M-CSF + LPS for 10 days. Pancreatic fibroblasts were cultured in the SiaJ setup with or without TGFβ for up to 13 days. The data are representative of 6 individual replicates plotted as mean +/- SEM. Statistical analysis was conducted using the Brown-Forsythe and Welch one-way ANOVA test (assuming normal distribution, but non-equal SDs). * = p < 0.05, ** = p < 0.01, *** = p < 0.001.
Fig 2: Accumulation of PRO-C3 levels in supernatant from human fibroblasts. A): pancreatic fibroblasts or B) lung fibroblasts (B) treated with 0-200 nM recombinant BIGH3. C): pancreatic fibroblast treated with 50 nM recombinant BIGH3 +/- anti-BIGH3 antibody or isotype control IgG. The data are representative of 6 individual replicates plotted as mean +/- SEM. Statistical analysis was conducted using the Brown-Forsythe and Welch one-way ANOVA test (assuming normal distribution, but non-equal SDs). * = p < 0.05, ** = p < 0.01, *** = p < 0.001.
Supplier Page from Abcam for Human Beta IG-H3 ELISA Kit