Fig 1: CRC Secretome Drives Matrix Metalloproteinase Signaling and Collagen DepositionCCSF exposure changes collagen degradation and deposition behaviors for both pericytes and fibroblasts.(A–C) SW480 conditioned media significantly increases MMPs two and nine expressions by fibroblasts. All CRC media upregulate MMP13 expression, with HCT116 media inducing the largest change.(D–F) Pericyte MMP2 expression is significantly downregulated with exposure to CCSFs, and additionally correlates with CRC cell line aggression.Pericyte MMP13 is underexpressed with CCSF exposure, and MMP9 shows no clear trend relative to media type.(G and H) HCT116 conditioned media significantly increases the quantity of collagen deposition by fibroblasts; pericytes follow the same trend but have no significant changes. Significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Data are represented as mean ± SEM n ≥ 3.
Fig 2: Time line of experiment for chondrocyte investigations. Human articular chondrocytes were seeded for ex vivo experiments in culture medium with 10% of FCS and grown until they reached 80% confluence. Cells were then incubated for 24 h in culture medium in the presence of 10% of FCS, NHS, or EHS before analyses of viability (XTT-24 h). Cells were then incubated for an additional 24 h, still in the presence of 10% of FCS, NHS, or EHS (culture medium being changed) but with or without IL-1β (1 ng/mL). Analyses of viability (XTT-48 h), NO, PGE2, and MMP-13 were carried out at the end of this new incubation period. FCS: fetal calf serum, NHS: naïve human serum, EHS: enriched human serum, IL-1β: Interleukin-1 β, NO: nitric oxide: PGE2: Prostaglandin E2, MMP-13: Metalloprotease-13.
Fig 3: MMP-13 protein released by human primary chondrocytes upon FCS (A) or human serum incubation (B). Incubation with FCS 10% served as control conditions for the validation of both the inflammatory model and the human serum incubation. Inflammatory stress was induced by the addition of recombinant human IL-1β (1 ng/mL). Values are presented as box and whisker plots, with min to max and group means above. Differences were considered statistically significant at p < 0.05, with * for p < 0.05; ** for p < 0.01; **** for p < 0.0001; and ns for p > 0.05. FCS: fetal calf serum (in white), NHS: naïve human serum (in grey), EHS: enriched human serum (in orange).
Supplier Page from Abcam for Human MMP13 ELISA Kit