Fig 1: Effect of B. coagulans spores, GBRS and synbiotic on immune markers in colon tissues and blood serum. Protein levels of cytokines including a IL-1α, b IL-1β, c IL-6, d IL-12, e TNF-α, f IFN-γ in proximal and distal colon explants as well as cytokine levels of g IL-1β, h IL-10, and i IL-12 in blood serum were analysed by Bio-plex. iNOS activity in colon tissues j measured by NOS activity assay and CRP levels in serum k by ELISA. Statistical significance among groups evaluated by one-way ANOVA followed by Tukey’s test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. DSS-control group and data expressed as mean ± SEM (n = 3 per group)
Fig 2: Effect of B. coagulans spores, PSCF and synbiotic on immune markers in colon tissues and blood serum. Protein levels of cytokines including (A) IL-1α, (B) IL-1β, (C) IL-6, (D) IL-12, (E) TNF-α, (F) IFN-γ in proximal and distal colon explants as well as cytokine levels of (G) IL-1β, (H) IL-10, and (I) IL-12 in blood serum were analysed by Bio-plex. iNOS activity in colon tissues (J) measured by NOS activity assay and CRP levels in serum (K) by ELISA. Statistical significance among groups evaluated by one-way ANOVA followed by Tukey’s test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. DSS-colitic group and data expressed as mean ± SEM (n = 3 per group).
Supplier Page from Abcam for Nitric Oxide Synthase Activity Assay Kit (Fluorometric)