Phase-Flow™ FITC BrdU Kit 370704 from BioLegend

Product Specs
ItemPhase-Flow™ FITC BrdU Kit
CompanyBioLegend
Price $703.00Supplier Page View Company Product Page
Catalog Number370704
Quantity100 tests
ApplicationsICFC - Quality tested
IsotypeMouse IgG1, kappa
Clone3D4
Detection TargetBrdU
ImmunogenIodouridine-conjugated ovalbumin
Source/Expression SystemMouse
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
TypeFlow Kit

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Heme enhances B-cell proliferation and plasma cell formation through reduced p21 and Rb expressionJ ImmunolMarch 18, 2026Herbey O Padilla-Quirarte, Anna K Kania, Nicolas V Janto, Bagdeser Akdogan-Ozdilek, Sakeenah L Hicks, Carly J Roman, Mansi Gupta, Jeremy M Boss, Christopher D Scharer(BioLegend: 103905) was used to stain dead cells, and Phase-Flow kit for proliferating cells (BioLegend: 370704). Intracellular staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (Invitrogen:

(2) ATM-dependent DNA damage response constrains cell growth and drives clonal hematopoiesis in telomere biology disordersJ Clin InvestApril 15, 2025Christopher M. Sande, Stone Chen, Dana V. Mitchell, Ping Lin, Diana M. Abraham, Jessie Minxuan Cheng, Talia Gebhard, Rujul J. Deolikar, Colby Freeman, Mary Zhou, et al.cytogenomic arrays. Cell cycle was analyzed with BrdU incorporation assay (Phase-Flow FITC BrdU Kit, catalog 370704; BioLegend). ATM knockdown was performed using 10 nM ON-TARGETplus-SMARTpool siRNAs to ATM or the Non-Targeting

(3) SUMOylation inhibition potentiates the glucocorticoid receptor to program growth arrest of acute lymphoblastic leukemia cellsOncogeneFebruary 14, 2025Emma Valima, Vera Varis, Kseniia Bureiko, Joanna K. Lempiäinen, Anna-Mari Schroderus, Laura Oksa, Olli Lohi, Tuure Kinnunen, Markku Varjosalo, Einari A. Niskanen, et al.normal rabbit IgG (12-370) was from Merck Millipore (Burlington, MA, USA); anti-BrdU A647 was from Biolegend (#370704, San Diego, CA, USA). Cell culture and compounds Human pre-B cell line NALM6 (ACC-128, DSMZ, Germany)

(4) WDR20 prevents hepatocellular carcinoma senescence by orchestrating the simultaneous USP12/46-mediated deubiquitination of c-MycProceedings of the National Academy of SciencesOctober 29, 2024Li Jiang, Xuxin Qi, Minshan Lai, Jiahao Zhou, Meng Yuan, Jieqiong You, Qiang Liu, Jinchang Pan, Luyao Zhao, Meidan Ying, et al.are listed in SI Appendix, Table S1. The 5-bromodeoxyuridine (BrdU) cell proliferation kit (Cat. No. 370704) for flow cytometry was obtained from Biolegend (CA, USA). The human cytokine array kit (Cat. No. ARY005B)

(5) Novel Methionine Aminopeptidase 2 Inhibitor M8891 Synergizes with VEGF Receptor Inhibitors to Inhibit Tumor Growth of Renal Cell Carcinoma ModelsMolecular cancer therapeuticsFebruary 1, 2024Friese-Hamim M, Ortiz Ruiz MJ, Bogatyrova O, Keil M, Rohdich F, Blume B, Leuthner B, Czauderna F, Hahn D, Jabs J, et al.cells were stained for 1 hour with 3.26 mmol/L BrdUrd using the Phase-Flow FITC BrdUrd Kit (BioLegend, #370704). Next, cells were fixed/stained according to the manufacturer's instructions using reagents supplied

(6) Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cellsMolecular psychiatryJune 1, 2023Simchi L, Gupta PK, Feuermann Y, Kaphzan H.with anti-BrdU antibody and 7-AAD utilizing the Phase-Flow™ BrdU Cell proliferation Kit (BioLegend #370704) according to the manufacturer’s protocol. We monitored the fluorescence labeling by FACS and analyzed

(7) Graft-versus-host disease is locally maintained in target tissues by resident progenitor-like T cellsImmunityFebruary 14, 2023Sacirbegovic F, Günther M, Greco A, Zhao D, Wang X, Zhou M, Rosenberger S, Oberbarnscheidt MH, Held W, McNiff J, et al.Cytofix/Cytoperm Kit with GolgiPlug,BD Biosciences,Cat#: BD555128 Phase Flow FITC BrdU Kit,BioLegend,Cat#: 370704 Chromium Single Cell 3’ Library and Gel Bead Kit,10X Genomics,Cat#: PN-1000075 Deposited data,Deposited

(8) Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell deathNature communicationsNovember 25, 2022Scur M, Mahmoud AB, Dey S, Abdalbarri F, Stylianides I, Medina-Luna D, Gamage GS, Woblistin A, Wilson ANM, Zein HS, et al.previously to obtain the AM. The cells were then processed using the BioLegend Phase-Flow kit (Cat#370704) as per the manufacturer’s instruction with no modification. The samples were then run on a BD-Fortessa

(9) RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variabilityLife science allianceAugust 1, 2022Gehling K, Parekh S, Schneider F, Kirchner M, Kondylis V, Nikopoulou C, Tessarz P.3.3.3 (Wickham, 2011). FACS analysis For the FACS analysis, we used the Phase-Flow FITC BrdU Kit (#370704; BioLegend) according to the manufacturer’s instructions with the following modifications. 0.5 μl/ml

(10) H3K27me3 Demethylase UTX Restrains Plasma Cell FormationJournal of immunology (Baltimore, Md. : 1950)April 15, 2022Kania AK, Price MJ, George-Alexander LE, Patterson DG, Hicks SL, Scharer CD, Boss JM.according to the manufactures’ protocols. BrdU analysis was performed using the Phase-Flow™ FITC BrdU Kit (370704; Biolegend). Flow cytometry was performed on a BD Fortessa X20 or BD Symphony A3 using FACSDiva and

(11) 5-aza-2'-deoxycytidine potentiates anti-tumor immunity in colorectal peritoneal metastasis by modulating ABC A9-mediated cholesterol accumulation in macrophagesTheranosticsJanuary 1, 2022Shi R, Zhao K, Wang T, Yuan J, Zhang D, Xiang W, Qian J, Luo N, Zhou Y, Tang B, et al.assay After two hours of Brdu pretreatment, cells were treated with the Phase-Flow™ FITC Brdu Kit (#370704, BioLegend). In brief, the cells were first fixed in Buffer A for 20 min and then washed with Buffer

(12) Inhibition of H3K27me3 Demethylases Promotes Plasmablast FormationImmunoHorizonsDecember 8, 2021Kania AK, Guo M, Scharer CD, Boss JM.analysis then performed using Phase-Flow FITC BrdU Kit following the manufacturer’s protocol (Biolegend 370704). Flow Cytometry Cells were resuspended at 106 per 100μl of FACS buffer (1X PBS, 1%BSA, and 2mM EDTA)

(13) An IRF4-MYC-mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In VivoJournal of immunology (Baltimore, Md. : 1950)October 1, 2021Patterson DG, Kania AK, Price MJ, Rose JR, Scharer CD, Boss JM.Staining of BrdU, Ki67, and 7AAD was achieved using the Phase-Flow BrdU Cell Proliferation Kit (Biolegend; 370704), substituting anti-BrdU for anti-Ki67 when desired. Intracellular pS6 staining was accomplished following

(14) SLAM family receptors control pro-survival effectors in germinal center B cells to promote humoral immunityThe Journal of experimental medicineMarch 1, 2021Zhong MC, Lu Y, Qian J, Zhu Y, Dong L, Zahn A, Di Noia JM, Karo-Atar D, King IL, Veillette A.62249). They were then processed for flow cytometry. For BrdU labeling, a kit from BioLegend (catalog no. 370704) was used. On day 7 after immunization, mice were injected i.p. with 1 mg BrdU (in 0.1 ml). After 2

(15) SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment PotentialBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow TransplantationApril 1, 2020Aerts-Kaya FSF, Visser TP, Pervin B, Mammadova A, Özyüncü Ö, Wagemaker G, Uçkan-Çetinkaya FD.

(16) Combined immunodeficiency caused by a loss-of-function mutation in DNA polymerase delta 1The Journal of allergy and clinical immunologyJanuary 1, 2020Cui Y, Keles S, Charbonnier LM, Julé AM, Henderson L, Celik SC, Reisli I, Shen C, Xie WJ, Schmitz-Abe K, et al.mononuclear cells (PBMCs) was performed with the Phase-Flow™ FITC BrdU Kit from Biolegend (Catalog No. 370704) following the manufacturer’s instructions. The same kit included 7-aminoactinomycin D (7-AAD) for staining

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Phase-Flow™ FITC BrdU Kit from BioLegend

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Phase-Flow™ FITC BrdU Kit from BioLegend

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