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Assay Kits
Assay Kit
Phase-Flow™ FITC BrdU Kit
Phase-Flow™ FITC BrdU Kit from BioLegend
Be the first to write a review!
Citations:
(16)
Supplier Page
Supplier Page from
BioLegend for
Phase-Flow™ FITC BrdU Kit
Description
Phase-Flow™ FITC BrdU Kit [3D4];
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Phase-Flow™ FITC BrdU Kit
Company
BioLegend
Price
$703.00
Supplier Page
View Company Product Page
Catalog Number
370704
Quantity
100 tests
Applications
ICFC - Quality tested
Isotype
Mouse IgG1, kappa
Clone
3D4
Detection Target
BrdU
Immunogen
Iodouridine-conjugated ovalbumin
Source/Expression System
Mouse
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Type
Flow Kit
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BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (16)
(1)
Heme enhances B-cell proliferation and plasma cell formation through reduced p21 and Rb expression
J Immunol
March 18, 2026
Herbey O Padilla-Quirarte, Anna K Kania, Nicolas V Janto, Bagdeser Akdogan-Ozdilek, Sakeenah L Hicks, Carly J Roman, Mansi Gupta, Jeremy M Boss, Christopher D Scharer
(
BioLegend
:
103905
) was used to stain dead cells, and Phase-Flow kit for proliferating cells (
BioLegend
:
370704
). Intracellular
staining
was performed using the Foxp3/Transcription Factor Staining Buffer Set (Invitrogen:
(2)
ATM-dependent DNA damage response constrains cell growth and drives clonal hematopoiesis in telomere biology disorders
J Clin Invest
April 15, 2025
Christopher M. Sande, Stone Chen, Dana V. Mitchell, Ping Lin, Diana M. Abraham, Jessie Minxuan Cheng, Talia Gebhard, Rujul J. Deolikar, Colby Freeman, Mary Zhou,
et al.
cytogenomic arrays. Cell cycle was analyzed with BrdU incorporation
assay
(
Phase-Flow FITC BrdU Kit
, catalog
370704
;
BioLegend
). ATM
knockdown
was performed using 10 nM ON-TARGETplus-SMARTpool siRNAs to ATM or the Non-Targeting
(3)
SUMOylation inhibition potentiates the glucocorticoid receptor to program growth arrest of acute lymphoblastic leukemia cells
Oncogene
February 14, 2025
Emma Valima, Vera Varis, Kseniia Bureiko, Joanna K. Lempiäinen, Anna-Mari Schroderus, Laura Oksa, Olli Lohi, Tuure Kinnunen, Markku Varjosalo, Einari A. Niskanen,
et al.
normal rabbit IgG
(
12-370
) was from
Merck Millipore
(Burlington, MA, USA); anti-BrdU A647 was from
Biolegend
(#
370704
, San Diego, CA, USA).
Cell culture
and compounds
Human
pre-B cell
line NALM6 (ACC-128, DSMZ, Germany)
(4)
WDR20 prevents hepatocellular carcinoma senescence by orchestrating the simultaneous USP12/46-mediated deubiquitination of c-Myc
Proceedings of the National Academy of Sciences
October 29, 2024
Li Jiang, Xuxin Qi, Minshan Lai, Jiahao Zhou, Meng Yuan, Jieqiong You, Qiang Liu, Jinchang Pan, Luyao Zhao, Meidan Ying,
et al.
are listed in SI Appendix, Table S1. The 5-bromodeoxyuridine (BrdU) cell proliferation kit (Cat. No.
370704
)
for flow cytometry
was obtained from
Biolegend
(CA, USA). The
human
cytokine array kit (Cat. No.
ARY005B
)
(5)
Novel Methionine Aminopeptidase 2 Inhibitor M8891 Synergizes with VEGF Receptor Inhibitors to Inhibit Tumor Growth of Renal Cell Carcinoma Models
Molecular cancer therapeutics
February 1, 2024
Friese-Hamim M, Ortiz Ruiz MJ, Bogatyrova O, Keil M, Rohdich F, Blume B, Leuthner B, Czauderna F, Hahn D, Jabs J,
et al
.
cells were stained for 1 hour with 3.26 mmol/L BrdUrd using the Phase-Flow FITC BrdUrd Kit (
BioLegend
, #
370704
). Next, cells were fixed/stained according to the manufacturer's instructions using reagents supplied
(6)
Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cells
Molecular psychiatry
June 1, 2023
Simchi L, Gupta PK, Feuermann Y, Kaphzan H.
with anti-BrdU antibody and 7-AAD utilizing the Phase-Flow™ BrdU Cell proliferation Kit (
BioLegend
#
370704
) according to the manufacturer’s protocol. We monitored the
fluorescence
labeling
by FACS and analyzed
(7)
Graft-versus-host disease is locally maintained in target tissues by resident progenitor-like T cells
Immunity
February 14, 2023
Sacirbegovic F, Günther M, Greco A, Zhao D, Wang X, Zhou M, Rosenberger S, Oberbarnscheidt MH, Held W, McNiff J,
et al
.
Cytofix/Cytoperm Kit with GolgiPlug,BD Biosciences,Cat#: BD555128
Phase Flow FITC BrdU Kit
,
BioLegend
,Cat#:
370704
Chromium Single Cell 3’ Library and Gel Bead Kit,10X Genomics,Cat#: PN-1000075 Deposited data,Deposited
(8)
Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death
Nature communications
November 25, 2022
Scur M, Mahmoud AB, Dey S, Abdalbarri F, Stylianides I, Medina-Luna D, Gamage GS, Woblistin A, Wilson ANM, Zein HS,
et al
.
previously to obtain the AM. The cells were then processed using the
BioLegend
Phase-Flow kit (Cat#
370704
) as per the manufacturer’s instruction with no modification. The samples were then run on a BD-Fortessa
(9)
RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variability
Life science alliance
August 1, 2022
Gehling K, Parekh S, Schneider F, Kirchner M, Kondylis V, Nikopoulou C, Tessarz P.
3.3.3 (Wickham, 2011). FACS analysis For the FACS analysis, we used the
Phase-Flow FITC BrdU Kit
(#
370704
;
BioLegend
) according to the manufacturer’s instructions with the following modifications. 0.5 μl/ml
(10)
H3K27me3 Demethylase UTX Restrains Plasma Cell Formation
Journal of immunology (Baltimore, Md. : 1950)
April 15, 2022
Kania AK, Price MJ, George-Alexander LE, Patterson DG, Hicks SL, Scharer CD, Boss JM.
according to the manufactures’ protocols. BrdU analysis was performed using the
Phase-Flow™ FITC BrdU Kit
(
370704
;
Biolegend
).
Flow cytometry
was performed on a BD Fortessa X20 or BD Symphony A3 using FACSDiva and
(11)
5-aza-2'-deoxycytidine potentiates anti-tumor immunity in colorectal peritoneal metastasis by modulating ABC A9-mediated cholesterol accumulation in macrophages
Theranostics
January 1, 2022
Shi R, Zhao K, Wang T, Yuan J, Zhang D, Xiang W, Qian J, Luo N, Zhou Y, Tang B,
et al
.
assay
After two hours of Brdu pretreatment, cells were treated with the
Phase-Flow™ FITC Brdu Kit
(#
370704
,
BioLegend
). In brief, the cells were first fixed in Buffer A for 20 min and then washed with Buffer
(12)
Inhibition of H3K27me3 Demethylases Promotes Plasmablast Formation
ImmunoHorizons
December 8, 2021
Kania AK, Guo M, Scharer CD, Boss JM.
analysis then performed using
Phase-Flow FITC BrdU Kit
following the manufacturer’s protocol (
Biolegend
370704
).
Flow Cytometry
Cells were resuspended at 106 per 100μl of FACS buffer (1X PBS, 1%BSA, and 2mM EDTA)
(13)
An IRF4-MYC-mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In Vivo
Journal of immunology (Baltimore, Md. : 1950)
October 1, 2021
Patterson DG, Kania AK, Price MJ, Rose JR, Scharer CD, Boss JM.
Staining
of BrdU,
Ki67
, and 7AAD was achieved using the Phase-Flow BrdU Cell Proliferation Kit (
Biolegend
;
370704
), substituting anti-BrdU for anti-
Ki67
when desired. Intracellular pS6
staining
was accomplished following
(14)
SLAM family receptors control pro-survival effectors in germinal center B cells to promote humoral immunity
The Journal of experimental medicine
March 1, 2021
Zhong MC, Lu Y, Qian J, Zhu Y, Dong L, Zahn A, Di Noia JM, Karo-Atar D, King IL, Veillette A.
62249
). They were then processed
for flow cytometry
. For
BrdU
labeling
, a kit from
BioLegend
(catalog no.
370704
) was used. On day 7 after
immunization
,
mice
were injected i.p. with 1 mg BrdU (in 0.1 ml). After 2
(15)
SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
April 1, 2020
Aerts-Kaya FSF, Visser TP, Pervin B, Mammadova A, Özyüncü Ö, Wagemaker G, Uçkan-Çetinkaya FD.
(16)
Combined immunodeficiency caused by a loss-of-function mutation in DNA polymerase delta 1
The Journal of allergy and clinical immunology
January 1, 2020
Cui Y, Keles S, Charbonnier LM, Julé AM, Henderson L, Celik SC, Reisli I, Shen C, Xie WJ, Schmitz-Abe K,
et al
.
mononuclear cells (PBMCs) was performed with the
Phase-Flow™ FITC BrdU Kit
from
Biolegend
(Catalog No.
370704
) following the manufacturer’s instructions. The same kit included 7-aminoactinomycin D (7-AAD) for
staining
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Phase-Flow™ FITC BrdU Kit from BioLegend
Supplier Page
View Company Product Page
$703.00
Product Specs
Company Info
Citations
Reviews
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