Phase-Flow(TM) FITC BrdU Kit 370704 from BioLegend

Product Specs
ItemPhase-Flow(TM) FITC BrdU Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number370704
Quantity100 tests
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
IsotypeIgG1, Kappa
Clone3D4
Antibody TypePrimary
Detection TargetBrdU
ImmunogenIodouridine-conjugated ovalbumin
Source/Expression SystemMouse

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BioLegend

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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cellsMolecular PsychiatryJune 1, 2023Lilach Simchi, Pooja Kri Gupta, Yonatan Feuermann, Hanoch Kaphzanwith anti-BrdU antibody and 7-AAD utilizing the Phase-Flow™ BrdU Cell proliferation Kit (BioLegend #370704) according to the manufacturer’s protocol. We monitored the fluorescence labeling by FACS and analyzed

(2) RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variabilityLife Science AllianceDecember 1, 2022Kristin Gehling, Swati Parekh, Farina Schneider, Marcel Kirchner, Vangelis Kondylis, Chrysa Nikopoulou, Peter Tessarz3.3.3 (Wickham, 2011). FACS analysis For the FACS analysis, we used the Phase-Flow FITC BrdU Kit (#370704; BioLegend) according to the manufacturer’s instructions with the following modifications. 0.5 μl/ml

(3) Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell deathNature communicationsNovember 25, 2022Scur M, Mahmoud AB, Dey S, Abdalbarri F, Stylianides I, Medina-Luna D, Gamage GS, Woblistin A, Wilson ANM, Zein HS, et al.previously to obtain the AM. The cells were then processed using the BioLegend Phase-Flow kit (Cat#370704) as per the manufacturer’s instruction with no modification. The samples were then run on a BD-Fortessa

(4) H3K27me3 Demethylase UTX Restrains Plasma Cell FormationThe Journal of ImmunologyApril 15, 2022Anna K. Kania, Madeline J. Price, Lou-Ella George-Alexander, Dillon G. Patterson, Sakeenah L. Hicks, Christopher D. Scharer, Jeremy M. Bossaccording to the manufactures’ protocols. BrdU analysis was performed using the Phase-Flow™ FITC BrdU Kit (370704; Biolegend). Flow cytometry was performed on a BD Fortessa X20 or BD Symphony A3 using FACSDiva and

(5) 5-aza-2′-deoxycytidine potentiates anti-tumor immunity in colorectal peritoneal metastasis by modulating ABC A9-mediated cholesterol accumulation in macrophagesTheranosticsJanuary 1, 2022Rongchen Shi et al.assay After two hours of Brdu pretreatment, cells were treated with the Phase-Flow™ FITC Brdu Kit (#370704, BioLegend). In brief, the cells were first fixed in Buffer A for 20 min and then washed with Buffer

(6) Inhibition of H3K27me3 Demethylases Promotes Plasmablast FormationImmunoHorizonsDecember 8, 2021Kania AK, Guo M, Scharer CD, Boss JM.analysis then performed using Phase-Flow FITC BrdU Kit following the manufacturer’s protocol (Biolegend 370704). Flow Cytometry Cells were resuspended at 106 per 100μl of FACS buffer (1X PBS, 1%BSA, and 2mM EDTA)

(7) An IRF4–MYC–mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In VivoThe Journal of ImmunologyOctober 1, 2021Dillon G. Patterson, Anna K. Kania, Madeline J. Price, James R. Rose, Christopher D. Scharer, Jeremy M. BossStaining of BrdU, Ki67, and 7AAD was achieved using the Phase-Flow BrdU Cell Proliferation Kit (Biolegend; 370704), substituting anti-BrdU for anti-Ki67 when desired. Intracellular pS6 staining was accomplished following

(8) SLAM family receptors control pro-survival effectors in germinal center B cells to promote humoral immunityThe Journal of experimental medicineMarch 1, 2021Zhong MC, Lu Y, Qian J, Zhu Y, Dong L, Zahn A, Di Noia JM, Karo-Atar D, King IL, Veillette A.62249). They were then processed for flow cytometry. For BrdU labeling, a kit from BioLegend (catalog no. 370704) was used. On day 7 after immunization, mice were injected i.p. with 1 mg BrdU (in 0.1 ml). After 2

(9) Combined immunodeficiency caused by a loss-of-function mutation in DNA polymerase delta 1Journal of Allergy and Clinical ImmunologyJanuary 1, 2020Ye Cui et al.mononuclear cells (PBMCs) was performed with the Phase-Flow™ FITC BrdU Kit from Biolegend (Catalog No. 370704) following the manufacturer’s instructions. The same kit included 7-aminoactinomycin D (7-AAD) for staining

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Phase-Flow(TM) FITC BrdU Kit from BioLegend

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Phase-Flow(TM) FITC BrdU Kit from BioLegend

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