ScanLater Western Blot Assay Kit from Molecular Devices

Product Specs
ItemScanLater Western Blot Assay Kit
CompanyMolecular Devices
Price Supplier Page View Company Product Page
Catalog NumberScanLater
QuantityEA

Add to Compare List

Molecular Devices

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: www.moleculardevices.com

(1) Does Ras/Rho Have Skin in the Game: The Importance of the Isoprenoid Biosynthesis Pathway in Merkel Cell Carcinoma Cell LinesCancers (Basel)May 13, 2026Louise N. Blaha, Nicole M. Derosia, Jeffrey D. Neighbors, Raymond J. Hohl1:1000: anti-RhoA (2117s), anti-Cdc42 (ab187643), anti-Rac1/2/3 (ab282581), and anti-vinculin (13901S). ScanLater Europium-conjugated anti-mouse or anti-rabbit secondary antibodies (Molecular Devices, San Jose, CA,

(2) Valproate independently activates Snf1, inhibits TORC1, and induces repression of INO1 transcription by increasing nuclear localization of Opi1Sci RepJuly 9, 2025Kendall C. Case, Lara Orkun, Miriam L. GreenbergImaging System (Invitrogen). Europium conjugated secondary antibodies were imaged with the SpectraMax i3 ScanLater cartridge (Molecular Devices); we found that dried membranes labeled with No-Stain Labeling Reagent

(3) PET imaging in rat brain shows opposite effects of acute and chronic alcohol exposure on phosphodiesterase-4B, an indirect biomarker of cAMP activityNeuropsychopharmacologyDecember 1, 2024Shiyu Tang, Sung Won Kim, Amanda Olsen-Dufour, Torben Pearson, Michael Freaney, Erick Singley, Madeline Jenkins, Nathaniel J. Burkard, Aaron Wozniak, Paul Parcon, et al.Bio-Rad Trans-Blot Turbo transfer system (Bio-Rad, San Francisco, CA). The membrane was blocked with the Scanlater blocking buffer (Molecular Devices, LLC, San Jose, California) for 1 hour at room temperature before

(4) Photo‐Responsive Decellularized Small Intestine Submucosa HydrogelsAdvanced Functional MaterialsSeptember 4, 2024Van Thuy Duong, Han Dang Nguyen, Ngoc Ha Luong, Chun‐Yi Chang, Chien‐Chi LinTrans-Blot Turbo Mini PVDF Transfer Packs (Biorad). Blotting procedures were carried out using the ScanLater™ Western Blot Assay Kit (Molecular Devices, USA). The membrane was blocked for an hour at RT using 1X blocking buffer. Subsequently,

(5) Viscoelastic stiffening of gelatin hydrogels for dynamic culture of pancreatic cancer spheroidsActa BiomaterialiaMarch 1, 2024Han D Nguyen, Chien-Chi LinCell Signaling Technology). After incubation with appropriate Europium-labeled secondary antibodies (Molecular Devices, ScanLater Eu-Goat anti-mouse IgG, R8208 or ScanLater Eu-Goat anti-Rabbit IgG, R8209), the membrane was scanned

(6) Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralizationCell chemical biologyJuly 20, 2023McConnell SA, Sachithanandham J, Mudrak NJ, Zhu X, Farhang PA, Cordero RJB, Wear MP, Shapiro JR, Park HS, Klein SL, et al.ChemiDoc Imaging System (BioRad). For Europium detection, membranes were imaged using the time-resolved fluorescence ScanLater Western Blot protocol on the SpectraMax iD5 (Molecular Devices). Indirect enzyme-linked immunosorbent assays

(7) RNAseq reveals extensive metabolic disruptions in the sensitive SF-295 cell line treated with schweinfurthinsScientific ReportsDecember 1, 2022J. S. Weissenrieder, J. D. Weissenkampen, J. L. Reed, M. V. Green, C. Zheng, J. D. Neighbors, D. J. Liu, Raymond J. Hohlintensity of color38,39. Western blotting Western blotting was carried out with the Molecular Devices ScanLater system (San Jose, CA), as described previously40. Briefly, cells were harvested in RIPA buffer with

(8) The PI3K inhibitor pictilisib and the multikinase inhibitors pazopanib and sorafenib have an impact on Rac1 level and migration of medulloblastoma in vitroJournal of Cellular and Molecular MedicineDecember 1, 2022Leonie F. Schoen, Rogerio B. Craveiro, Torsten Pietsch, Thomas Moritz, Anja Troeger, Silvia Jordans, Dagmar Dilloo(Bio‐Rad). Blots from Figure 1D were analysed with the SpectraMax i3X (Molecular Devices) equipped with a ScanLater module in high resolution scan. Densitometry of Western blots was performed using ImageJ 1.53c. 2.9

(9) Activation of glucagon-like peptide-1 receptors reduces the acquisition of aggression-like behaviors in male miceTranslational psychiatryOctober 13, 2022Vestlund J, Zhang Q, Shevchouk OT, Hovey D, Sebastian L, Westberg L, Jerlhag E.Country)",Dilution/incubation TPH2,"Rabbit anti-TPH2 LS‑C346253, Lifespan Bio, Seattle, WA, USA",1:500 in 5% skim milk/ overnight,"Scanlater Eu-labeled Goat Anti- Rabbit lgG R8029 Molecular Devices, Sunnyvale, CA, USA",1:5000 in 5% skim milk/

(10) Genetic and Drug Inhibition of LDH-A: Effects on Murine GliomasCancersMay 6, 2022Maeda M, Ko M, Mane MM, Cohen IJ, Shindo M, Vemuri K, Serganova I, Blasberg R.#A2103) at a 1:5000 dilution. Bound primary antibodies were visualized with Eu-labeled antibody using ScanLater Western Blot Assay kit and SpectraMax ID5 (Molecular Devices, San Jose, CA, USA). 2.4. LDH Enzyme Activity and Lactate-Glo

(11) LDH-A-Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth ResponsesCancersMay 6, 2022Shindo M, Maeda M, Myat K, Mane MM, Cohen IJ, Vemuri K, Albeg AS, Serganova I, Blasberg R.an enhanced chemiluminescence reagent (Western Lightning-ECL) or with an Eu-labelled antibody using ScanLater Western Blot Assay kit and SpectraMax ID5 (Molecular Devices, San Jose, CA, USA). 2.4. LDH Enzyme Activity Total LDH enzyme

(12) Effect of Structural Changes Induced by Deletion of (54)FLRAPSWF(61) Sequence in αB-crystallin on Chaperone Function and Anti-Apoptotic ActivityInternational journal of molecular sciencesOctober 5, 2021Mahalingam S, Karmakar S, Santhoshkumar P, Sharma KK.incubated with primary protein-specific antibodies overnight at 4 °C. The membranes were incubated with the ScanLater Eu-labeled anti-rabbit or anti-mouse secondary antibodies (Molecular Devices) for 2 h. Blots were washed,

(13) Valproate activates the Snf1 kinase in Saccharomyces cerevisiae by decreasing the cytosolic pHJournal of Biological ChemistryOctober 1, 2021Michael Salsaa, Kerestin Aziz, Pablo Lazcano, Michael W. Schmidtke, Maureen Tarsio, Maik HÃ¼ttemann, Christian A. Reynolds, Patricia M. Kane, Miriam L. Greenbergdetected using a SpectraMax i3x multimode microplate reader with MiniMax 300 imaging cytometer and ScanLater Western blotting cartridge (Molecular Devices). Membranes were visualized with the SoftMax Pro software. ATP

(14) Antipsychotic drugs elicit cytotoxicity in glioblastoma multiforme in a calcium-dependent, non-D(2) receptor-dependent, mannerPharmacology research & perspectivesMay 1, 2021Weissenrieder JS, Reed JL, Moldovan GL, Johnson MT, Trebak M, Neighbors JD, Mailman RB, Hohl RJ.experiment). 2.6 Immunoblotting Western blot analysis was carried out as described previously using the ScanLater (Molecular Devices, San Jose, CA, USA) system. 13 Membranes were incubated with primary antibodies

(15) Effects of sub-chronic amylin receptor activation on alcohol-induced locomotor stimulation and monoamine levels in micePsychopharmacology (Berl)November 1, 2020Aimilia Lydia Kalafateli, Cajsa Aranäs, Elisabet Jerlhagfinal secondary antibody solutions were as follows: 1:5000 in TBS-T of Eu-labelled goat anti-mouse ScanLater™ (Molecular Devices, San Jose, CA, USA) was used against a-COXIV, and 1:5000 in TBS-T of Eu-labelled

(16) Low dose HSP90 inhibition with AUY922 blunts rapid evolution of metastatic and drug resistant phenotypes induced by TGF-β and paclitaxel in A549 cellsBiomedicine & PharmacotherapySeptember 1, 2020Nickolas A. Bacon, Isabel Larre, Abdalla A. Lawag, Carlen Merritt, Mackinzie Smith, Matthew Rosolen, Vincent E. Sollars(Fitzgerald Industries®, cat# 10R-G109a). Species-specific europium-conjugated secondary antibody (ScanLater goat anti mouse; part#: R7562; Molecular Devices®) was used. Afterward, membranes were washed with TBS-T

(17) IODVA1, a guanidinobenzimidazole derivative, targets Rac activity and Ras-driven cancer modelsPloS oneJanuary 1, 2020Gasilina A, Premnauth G, Gurjar P, Biesiada J, Hegde S, Milewski D, Ma G, Kalin TV, Merino E, Meller J, et al.processed with ECL (film or Bio-Rad chemiluminescent system) or imaged on the SpectraMax i3 platform with ScanLater module (Molecular Devices). Retrovirus production and transduction pBabe puro HRasG12V plasmid was

(18) The Dopamine D2 Receptor Contributes to the Spheroid Formation Behavior of U87 Glioblastoma CellsPharmacologyJanuary 1, 2020Weissenrieder JS, Reed JL, Green MV, Moldovan GL, Koubek EJ, Neighbors JD, Hohl RJ.spheroid counts. Immunoblotting Western blotting was performed as previously described [22] using the ScanLater (Molecular Devices, San Jose, CA, USA) visualization system, and 4–12% bis/tris gels (Life Technologies, Carlsbad,

(19) Unbiased peptoid combinatorial cell screen identifies plectin protein as a potential biomarker for lung cancer stem cellsScientific ReportsDecember 1, 2019Aaron C. Raymond, Boning Gao, Luc Girard, John D. Minna, D. Gomika Udugamasooriyawere transferred to a nitrocellulose membrane. The membrane was then blotted and visualized using the ScanLater Western Blot Kit (Molecular Devices, Sunnyvale, CA) as follows: the membrane is blocked for 1 hour in

(20) Dietary advanced glycation end-product consumption leads to mechanical stiffening of murine intervertebral discsDisease Models & MechanismsDecember 1, 2018Divya Krishnamoorthy, Robert C. Hoy, Devorah M. Natelson, Olivia M. Torre, Damien M. Laudier, James C. Iatridis, Svenja Illien-JüngerCA, USA) for 1 h at room temperature. After washing, the membranes were dried and scanned using the ScanLater (Molecular Devices) Western Blot cartridge in a SpectraMax i3 system (Molecular Devices). Membranes

Be the First to Write a Review!