Lactate-Glo Assay J5022 from Promega

Product Specs
ItemLactate-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ5022
Quantity50ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Mitochondrial membrane junction-mediated ATP channeling drives activity-dependent glucose metabolismbioRxivDecember 19, 2025Dengbao Yang, Gemma Molinaro, Nadine Nijem, Chuanhai Zhang, Dylen Penny, Meijuan Bai, Mei-Jung Lin, Xiaodong Wen, Salvador Pena, Janaka Wansapura, et al.cells and the buffer. The lactate levels in buffer were measured by a Lactate-Glo Assay kit (Promega, J5022) following manufacturer’s protocol. Nucleotide quantitation Primary brown adipocytes suspended in

(2) Bovine adipose mitochondrial adaptation and a potential lactate-ketone toggle in early lactationFront Vet SciDecember 3, 2025Nial J. O'Boyle, Miguel Chirivi, Ursula Abou-Rjeileh, David Salcedo-Tacuma, José M. Dos Santos Neto, Crystal Prom, Jair Parales-Girón, Adam L. Lock, Reinhard Stöger, Lisa Chakrabarti, et al.further analysis. L-Lactate detection and quantification was performed using the Lactate-Glo Assay (J5022; Promega) following manufacturer’s protocol. Briefly, plasma samples were thawed on ice and diluted

(3) Effects of 810 nm treatments in acute myofiber contraction of C2C12 myotubesPloS oneJanuary 1, 2025Cheema N, Pham L, Nazarian A, Ghag N, Wise E, Fuchs C, Anderson RR, Tam J.and LDH release. Lactate levels were immediately determined via the Lactate-Glo kit (Promega Cat # J5022) and LDH levels were measured via Cytotoxicity Detection Kit (LDH) (Roche Cat # 11644793001). Glucose

(4) A pH-Sensitive Fluorescent Chemosensor Turn-On Based in a Salen Iron (III) Complex: Synthesis, Photophysical Properties, and Live-Cell Imaging ApplicationMoleculesOctober 24, 2023Nicole Nilo, Mauricio Reyna-Jeldes, Alejandra A. Covarrubias, Claudio Coddou, Vania Artigas, Mauricio Fuentealba, Luis F. Aguilar, Marianela Saldías, Marco Melladowas removed, and intracellular lactate measurements were performed using the Lactate-Glo Assay kit (J5022; Promega, Madison, WI, USA) following manufacturer’s instructions. To establish intracellular lactate

(5) Small molecule anion carriers facilitate lactate transport in model liposomes and cellsiScienceOctober 20, 2023Daniel Alonso-Carrillo, Alain Arias-Betancur, Israel Carreira-Barral, Pere Fontova, Vanessa Soto-Cerrato, María García-Valverde, Ricardo Pérez-Tomás, Roberto Quesadacommercial assays,Critical commercial assays,Critical commercial assays ,, Lactate-Glo™ Assay Kit,Promega,J5022 Pierce™ BCA Protein Assay Kit,Thermo Fisher Scientific,23227 ,, Deposited data,Deposited data,Deposited

(6) HSP90B1-mediated plasma membrane localization of GLUT1 promotes radioresistance of glioblastomasThe Journal of Biomedical ResearchSeptember 28, 2023Yanhui Li, Yuqian Ge, Mengjie Zhao, Fangshu Ding, Xiuxing Wang, Zhumei Shi, Xin Ge, Xiefeng Wang, Xu Qiancounted in a duplicate sample. Lactate production assay A lactate production assay kit (Promega, J5022) was used following the manufacturer's instructions. Briefly, the attached GBM cells (1 × 10 4) and

(7) Paralog-dependent isogenic cell assay cascade generates highly selective SLC16A3 inhibitorsCell chemical biologyAugust 17, 2023Dvorak V, Casiraghi A, Colas C, Koren A, Tomek T, Offensperger F, Rukavina A, Tin G, Hahn E, Dobner S, et al.CellTiter-Glo® Luminescent Cell Viability Assay,Promega,Cat# G7572 Nano-Glo® HiBiT Lytic Detection System,Promega,Cat# N3040 Lactate-Glo™ Assay,Promega,Cat# J5022 Q5® Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0554S ,, Deposited data,Deposited data,Deposited

(8) MCT4-dependent lactate secretion suppresses antitumor immunity in LKB1-deficient lung adenocarcinomaCancer cellJuly 10, 2023Qian Y, Galan-Cobo A, Guijarro I, Dang M, Molkentine D, Poteete A, Zhang F, Wang Q, Wang J, Parra E, et al.and resuspended in PBS. Lactate level and glucose level were detected with Lactate-Glo™ Assay kit (J5022) and Glucose-Glo™ Assay (J6021, Promega) according to the manufactures’ instruction. Metabolic assays Cell

(9) Role of myeloid cells in system-level immunometabolic dysregulation during prolonged successful HIV-1 treatmentAIDSJune 1, 2023Sara Svensson Akusjärvi, Shuba Krishnan, Anoop T. Ambikan, Flora Mikaeloff, Sivasankaran Munusamy Ponnan, Jan Vesterbacka, Magda Lourda, Piotr Nowak, Anders Sönnerborg, Ujjwal Neogicells were used for metabolite detection in duplicates for each sample. Lactate-Glo assay (Promega, #J5022; Promega, Madison, Wisconsin, USA), Glutamate-Glo assay (Promega, #J7022), and Glucose-Glo assay (Promega,

(10) Characterizing the metabolic role of STAT3 in canine osteosarcomaVeterinary and Comparative OncologyDecember 1, 2022Heather L. Gardner, Joelle M. Fenger, Ryan D. Roberts, Cheryl A. LondonIntracellular lactate Intracellular lactate was determined enzymatically using a Lactate‐Glo Assay (Promega: #J5022) per manufacturer recommendations. Twenty‐thousand cells were seeded in opaque 96‐well plates and incubated

(11) Dose-related shifts in proteome and function of extracellular vesicles secreted by fetal neural stem cells following chronic alcohol exposureHeliyonNovember 1, 2022Dae D. Chung, Marisa R. Pinson, Amanda H. Mahnke, Nihal A. Salem, Khang T. Le, Elizabeth A. Payne, Tenley E. Lehman, Susan T. Weintraub, Rajesh C. Mirandathe alamarBlue assay. 4.13 Lactate-Glo glycolysis assay The Lactate-Glo Assay (Promega; Catalog #J5022; Madison, WI/USA) was used according to the manufacturer's instructions to measure the glycolysis rate

(12) H3K18 lactylation marks tissue-specific active enhancersGenome biologyOctober 3, 2022Galle E, Wong CW, Ghosh A, Desgeorges T, Melrose K, Hinte LC, Castellano-Castillo D, Engl M, de Sousa JA, Ruiz-Ojeda FJ, et al.measurement Intracellular and extracellular lactate concentration was measured using the Lactate GloTM Assay (Promega, J5022). Cells were seeded in a 96-well plate for lactate measurement. At the desired time point, media was

(13) Intestinal tissue-resident T cell activation depends on metabolite availabilityProceedings of the National Academy of Sciences of the United States of AmericaAugust 23, 2022Konjar Š, Ferreira C, Carvalho FS, Figueiredo-Campos P, Fanczal J, Ribeiro S, Morais VA, Veldhoen M.enzymatically in 96-well plates according to manufacturer’s specifications (Lactate-Glo Assay, Promega, J5022). Briefly, cells were lysed with 0.6N HCl. Cell lysates were neutralized with 1 M Tris-base and incubated

(14) Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signalingiScienceJanuary 21, 2022Akusjärvi SS, Ambikan AT, Krishnan S, Gupta S, Sperk M, Végvári Á, Mikaeloff F, Healy K, Vesterbacka J, Nowak P, et al.commercial assays,Critical commercial assays ,, Glucose-Glo,Promega,Cat#J6022 Lactate Glo,Promega,Cat#J5022 Glutamine/Glutamate Glo,Promega,Cat#J8021 ,, Deposited data,Deposited data,Deposited data ,, Metabolomics

(15) DHHC9-mediated GLUT1 S-palmitoylation promotes glioblastoma glycolysis and tumorigenesisNature communicationsOctober 7, 2021Zhang Z, Li X, Yang F, Chen C, Liu P, Ren Y, Sun P, Wang Z, You Y, Zeng YX, et al.levels of lactate and glucose in the culture medium were determined by kits obtained from Promega (#J5022) and Sigma (#GAGO20), respectively, according to the manufacturer’s instructions. Glucose consumption

(16) Genome-Wide Methylation Mapping Using Nanopore Sequencing Technology Identifies Novel Tumor Suppressor Genes in Hepatocellular CarcinomaInternational journal of molecular sciencesApril 11, 2021Davenport CF, Scheithauer T, Dunst A, Bahr FS, Dorda M, Wiehlmann L, Tran DDH.plates according to the manufacturer’s specifications (Lactate-Glo Assay, Promega (Madison, WI, USA), J5022). A total of 15000 cells/well were seeded for each measurement. Cell lysates were neutralized with 7.5

(17) Pathway-based classification of glioblastoma uncovers a mitochondrial subtype with therapeutic vulnerabilitiesNature cancerFebruary 1, 2021Garofano L, Migliozzi S, Oh YT, D'Angelo F, Najac RD, Ko A, Frangaj B, Caruso FP, Yu K, Yuan J, et al.was performed using Glucose Uptake-Glo Assay (PROMEGA, no. J1342), Lactate-Glo Assay (PROMEGA, no. J5022), Glutamine/Glutamate-Glo Assay (PROMEGA, no. J5022) and Triglyceride-Glo Assay (PROMEGA, no. J3161),

(18) Nitric Oxide Modulates Metabolic Remodeling in Inflammatory Macrophages through TCA Cycle Regulation and Itaconate AccumulationCell reportsJuly 2, 2019Bailey JD, Diotallevi M, Nicol T, McNeill E, Shaw A, Chuaiphichai S, Hale A, Starr A, Nandi M, Stylianou E, et al.hours. Lactate assay Extracellular lactate was determined using a Lactate-Glo assay kit (Promega, J5022). Media supernatants from 1x106 cells were diluted at 1:50 in PBS and 50 μL were plated in duplicate

(19) Dual Inhibition of the Lactate Transporters MCT1 and MCT4 Is Synthetic Lethal with Metformin due to NAD+ Depletion in Cancer CellsCell reportsDecember 11, 2018Benjamin D, Robay D, Hindupur SK, Pohlmann J, Colombi M, El-Shemerly MY, Maira SM, Moroni C, Lane HA, Hall MN.enzymatically in 96-well plates according to manufacturer’s specifications (Lactate-Glo Assay, Promega, J5022). 15000 cells/well or 30000 cells/well were seeded respectively for HeLa and HAP1. After compound incubation,

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Lactate-Glo Assay

Lactate-Glo Assay from Promega

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Lactate-Glo Assay from Promega

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