Lactate-Glo Assay J5021 from Promega

Product Specs
ItemLactate-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ5021
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

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Website: http://www.promega.com

(1) Class I HDAC inhibition enhances the stem-like memory properties of CRISPR-engineered CAR T cells in neuroblastomaMol Ther OncolJune 18, 2026Dan Cappabianca, Mahmoud Toulany, Seth Zima, Amanda Shea, Jolanta Vidugiriene, Anthony Lauer, Kayla Sylvester, Varun Gnanasekar, Sean Foster, Rithvik Turaga, et al.glutamate levels were measured using Lactate-Glo, Malate-Glo, and Glutamine/Glutamate-Glo (cat. nos. J6021, J5021, JE9100, and J8021; Promega, Madison, WI), respectively. For each donor, inhibitor-treated samples were

(2) Metabolic assessment of iPSC-derived neurons under ketone-enriched condition: Ketone sensor development and BHB-driven metabolic adaptationiScienceMay 15, 2026Rohollah Nasiri, Farzaneh Fayazbakhsh, Reyhaneh Sanei, Tingting Wu, Nayere Taebnia, Rouhollah Habibey, Omid Fotouhi, John Cognetti, Volker M. Lauschke, Aman Russom, et al.,Thermo Fisher Scientific,Cat# P11496 Glucose Glo Assay Kit,Promega,Cat# J6021 Lactate Assay Kit,Promega,Cat# J5021 TaqMan Gene Expression Assays,Thermo Fisher Scientific, SLC16A1,Thermo Fisher Scientific,Hs00560375_g1 OXCT1,Thermo

(3) β-Glucan training induces stimulus-dependent immune and metabolic modulation in head kidney leukocytes that persists for several days in Atlantic salmon (Salmo salar L.)Front ImmunolMay 7, 2026Thinh Hoang Nhan, Lluis Tort, Carlo C. Lazadolactate quantification Intracellular lactate was quantified using the Lactate-Glo™ Assay (Promega; Cat: J5021) according to the manufacturer’s protocol for cell lysates. After secondary stimulation (see section

(4) Calcium signals shape metabolic control of H3K27ac and H3K18la to regulate EGAbioRxivApril 25, 2026Virginia Savy, Paula Stein, Don Delker, Martín A. Estermann, Brian N. Papas, Zongli Xu, Lenka Radonova, Carmen J. WilliamsIndex Kit v2 Set B (96 Indices, 384 Samples)",Illumina,15052164 TG-131–2001 Lactate-Glo™ Assay,Promega,J5021 Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro,Vazyme,TD904 TruePrep® Index Kit V2 for Illumina,Vazyme,TD202 VAHTS

(5) Discovery and Preclinical Validation of a Clinically Optimized Mitochondrial Complex I Modulator for Alzheimer’s DiseasebioRxivApril 17, 2026Sergey Trushin, Thi Kim Oanh Nguyen, Mark Ostroot, Alexander Galkin, Toshihiko Nambara, Wenyan Lu, Takahisa Kanekiyo, Graham Johnson, Eugenia TrushinaGSH/GSSG-Glo™ Assay (Promega, V6612). Lactate levels were determined using the Lactate-Glo™ Assay (Promega, J5021). Antioxidant capacity of C273 was determined using the Antioxidant Assay kit (Sigma-Aldrich, MAK334). In

(6) Aging murine peritoneal macrophages undergo female-specific remodeling driven by hormone-dependent and independent mechanismsbioRxivApril 12, 2026Ryan J. Lu, Shiqi Chen, Minhoo Kim, Nirmal K. Sampathkumar, Evelyn H. Lee, Amy Christensen, Eric E. Wang, Isabella Y. Lau, Shivay Parihar, Charan K. Ravikumar, et al.mitochondrial function148. To measure the amount of lactate in the condition media, Lactate-Glo (Promega #J5021) was used following to the manufacturer’s protocol and luminescence values were collected using Promega

(7) Aerobic glycolysis and lactate regulate histone H3K18Lactylation occupancy to fine-tune gene expression in developing and mature retinabioRxivApril 12, 2026Mohita Gaur, Xulong Liang, Matthew J. Brooks, Ke Jiang, Anjani Kumari, Milton A. English, Paolo Cifani, Maria C. Panepinto, Jacob Nellissery, Robert N. Fariss, et al.performed on different age groups of mice (P2, P6, P10, P14, P28) using Promega Lactate-Glo (Promega J5021) assay according to the manufacturer’s instructions. Retina samples was homogenized in 1ml homogenization

(8) Lactate and histone H3K18 lactylation are associated with metabolic control of gene expression in the retinaPLoS GenetApril 8, 2026Mohita Gaur, Matthew J. Brooks, Xulong Liang, Ke Jiang, Anjani Kumari, Milton A. English, Paolo Cifani, Maria C. Panepinto, Jacob Nellissery, Robert N. Fariss, et al.P28) as well as on the conditioned medium in the retinal explants using Promega Lactate-Glo (Promega J5021) assay according to the manufacturer’s instructions. Retina samples was homogenized in 1 ml homogenization

(9) Hepatitis C Virus (HCV)—Mediated Activation of Hexokinase Domain-Containing Protein 1 (HKDC1) Promotes Hexokinase Activity and Metabolic ReprogrammingVirusesMarch 31, 2026Hope K. Fiadjoe, Amani Doyle, In-Woo Park, Pankaj ChaudharyPyruvate Assay Extracellular lactate and pyruvate levels were measured using the Lactate-Glo™ Assay (#J5021, Promega Corporation, Madison, WI, USA) and Pyruvate-Glo™ Assay (#J4051, Promega Corporation, Madison,

(10) Glycogen and lactate metabolism in mouse fetal Sertoli cells sustain the germ lineCell ReportsMarch 24, 2026Martín A. Estermann, Joseph Sheheen, Sara A. Grimm, Boris Tezak, Yu-Ying Chen, Tsuyoshi Morita, Humphrey H.-C. H.-C. Yao, Blanche Capelp-value <0.05. Lactate measurement Lactate concentration was measured using the Lactate-Glo Assay (J5021, Promega). Briefly, single cultured gonads or E12.5 Ppp1r3c control or cKO gonadal pairs were dissected

(11) Extracellular matrix signals promote actin-dependent mitochondrial elongation and activityiScienceMarch 20, 2026Priya Gatti, Pritha Mukherjee, Priyanka Dey Talukdar, Wesley Freppel, Anahita Kasmaie, Joseph Kanou, Laurent Chatel-Chaix, Urmi Chatterji, Marc Germainduplicate and the volume adjusted to 50 μL with PBS. Subsequently, 50 μL of Lactate reagent from Promega (#J5021) was added and incubated for 10 min at room temperature. The resulting absorbance was recorded at a

(12) NSD1-Mediated PPARγ Methylation Enhances PTEN Activity to Suppress Glycolysis and Tumor Progression in Endometrial CancerCancer ResMarch 2, 2026Han Tao, Ye Gu, Xiaojun Wang, Yeli Sun, Luhui Wang, Yilin Dai, Mengyue Zhu, Zhiyi Hu, Yiran Li, Mengwen Kong, et al.normalized to total cell number. For tissue-based lactate detection, the Lactate-Glo Assay (Promega, cat. #J5021) was employed. Tissue samples were homogenized and processed according to the manufacturer’s instructions,

(13) Brief pulses of high-level fluid shear stress enhance metastatic potential and rapidly alter the metabolism of cancer cellsCell ReportsFebruary 24, 2026Amanda N. Pope, Devon L. Moose, Guy O. Hudson, Hank R. Weresh, Marion R. Dykstra, Aabha Y. Joshi, Patrick Breheny, Eric B. Taylor, Michael D. Henryvalues. Lactate production The rate of lactate production was measured using Lactate-Glo assay kit (Promega, Cat#J5021). Our cancer cell lines were exposed to FSS or held in static conditions, washed with PBS, then immediately

(14) KRAS-dependent glycolytic reprogramming of endothelial cells in sporadic arteriovenous malformationsEMBO Molecular MedicineFebruary 18, 2026Ruilin Wu, Negar Khosraviani, Ann Mansur, Emilie Boudreau, Gabrielle E Largoza, Suejean Park, Dakota Gustafson, Sneha Raju, Crizza Ching, Amira Klip, et al.condition. Lactate-Glo™ assay Extracellular lactate was measured using the Lactate-Glo™ Assay Kit (Promega, Cat #: J5021) following the manufacturer’s instructions. Following serum/glucose starvation as described above for

(15) Differential roles of the type I and II secretion systems for the intracellular ABC141 Acinetobacter baumannii infection, which elicits an atypical hypoxia response in endothelial cellsPLOS PathogensFebruary 9, 2026Charline Debruyne, Landon Hodge, Karsten Hokamp, Anna S. Ershova, Carsten Kröger, Suzana P. Salcedolevels were determined with the Glucose-Glo Assay (Promega, J6021) and the Lactate-GloTAssay (Promega, J5021) following the manufacturer’s instructions. Briefly, after 24 h infection, cells were washed with cold

(16) Targeting metabolic vulnerabilities: REV-ERB agonist SR9009 potentiates sorafenib efficacy in liver cancerCell Death DiscoveryJanuary 19, 2026Silvia Sabbioni, Paola Guerriero, Ram C. Shankaraiah, Laura Masatti, Angelo Michilli, Cristian Bassi, Lucilla D’Abundo, Farzaneh Moshiri, Bruno De Siena, Edi Simoni, et al.associated with changes in cell membrane integrity and cellular ATP levels. The Lactate-Glo™ Assay (#J5021, Promega, Madison, WI, USA), a bioluminescent detection system that couples lactate oxidation and NADH

(17) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega, J5021). Thymocytes were isolated from untreated and TBI-treated mice and incubated in RPMI with 5 mM sodium

(18) Patient-derived colon epithelial organoids reveal lipid-related metabolic dysfunction in pediatric ulcerative colitisNat CommunDecember 10, 2025Babajide A. Ojo, Ying Zhu, Lyong Heo, Sejal R. Fox, Yanli Qiao, Amanda Waddell, Maria E. Moreno-Fernandez, Marielle Gibson, Tracy Tran, Ashley L. Dunn, et al.glucose and lactate were measured using the Glucose-Glo (Promega, #J6021) and Lactate-Glo (Promega, #J5021) assays, respectively. Samples were diluted 50X in ice-cold PBS, and the measurements continued according

(19) Microbial metabolite indole-3-propionic acid drives mitochondrial respiration in CD4(+) T cells to confer protection against intestinal inflammationNature metabolismDecember 1, 2025Li Q, de Oliveira Formiga R, Puchois V, Creusot L, Ahmad AH, Amouyal S, Campos-Ribeiro MA, Zhao Y, Harris DMM, Lasserre F, et al.collected and analysed for lactate concentration using a commercially available assay kit (Promega, J5021), following the manufacturer’s protocol. MitoTracker Green/Deep Red and ROS production For mitochondria

(20) Small Extracellular Vesicles Secreted by Cisplatin-Resistant Neuroblastoma Cells Increase Lactate Secretion and Alter Metabolic Pathways in Primary Human Umbilical Vein Endothelial Cells (HUVECs)J Pers MedDecember 1, 2025Thomas Frawley, Lin Ma, Muhammad Zainul Arifin, Dan Wu, Alysia Scott, Brenton Cavanagh, Donal F. O'Shea, Vadim Zhernovkov, Mi Liu, Marco P. Monopoli, et al.1.53f51). 2.14. Lactate Secretion The lactate secretion assay utilised the Lactate-Glo™ Assay (Promega, Cat #J5021, Madison, WI, USA) according to the manufacturer’s guidelines. Conditioned media were collected from

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1/25/2024Rapid Assay From Promega for Detecting Lactate Secretion Kateryna MurlanovaWe measure cell culture lactate (secreted extracellular lactate) to assess energy metabolism changes, specifically glycolysis, in cultured immunomagnetically isolated astrocytes.

Lactate-Glo Assay

Lactate-Glo Assay from Promega

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Lactate-Glo Assay from Promega

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