Lactate-Glo Assay J5021 from Promega

Product Specs
ItemLactate-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ5021
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

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Website: http://www.promega.com

(1) Class I HDAC inhibition enhances the stem-like memory properties of CRISPR-engineered CAR T cells in neuroblastomaMol Ther OncolJune 18, 2026Dan Cappabianca, Mahmoud Toulany, Seth Zima, Amanda Shea, Jolanta Vidugiriene, Anthony Lauer, Kayla Sylvester, Varun Gnanasekar, Sean Foster, Rithvik Turaga, et al.glutamate levels were measured using Lactate-Glo, Malate-Glo, and Glutamine/Glutamate-Glo (cat. nos. J6021, J5021, JE9100, and J8021; Promega, Madison, WI), respectively. For each donor, inhibitor-treated samples were

(2) Lactate and histone H3K18 lactylation are associated with metabolic control of gene expression in the retinaPLoS GenetApril 8, 2026Mohita Gaur, Matthew J. Brooks, Xulong Liang, Ke Jiang, Anjani Kumari, Milton A. English, Paolo Cifani, Maria C. Panepinto, Jacob Nellissery, Robert N. Fariss, et al.P28) as well as on the conditioned medium in the retinal explants using Promega Lactate-Glo (Promega J5021) assay according to the manufacturer’s instructions. Retina samples was homogenized in 1 ml homogenization

(3) Hepatitis C Virus (HCV)—Mediated Activation of Hexokinase Domain-Containing Protein 1 (HKDC1) Promotes Hexokinase Activity and Metabolic ReprogrammingVirusesMarch 31, 2026Hope K. Fiadjoe, Amani Doyle, In-Woo Park, Pankaj ChaudharyPyruvate Assay Extracellular lactate and pyruvate levels were measured using the Lactate-Glo™ Assay (#J5021, Promega Corporation, Madison, WI, USA) and Pyruvate-Glo™ Assay (#J4051, Promega Corporation, Madison,

(4) Extracellular matrix signals promote actin-dependent mitochondrial elongation and activityiScienceMarch 20, 2026Priya Gatti, Pritha Mukherjee, Priyanka Dey Talukdar, Wesley Freppel, Anahita Kasmaie, Joseph Kanou, Laurent Chatel-Chaix, Urmi Chatterji, Marc Germainduplicate and the volume adjusted to 50 μL with PBS. Subsequently, 50 μL of Lactate reagent from Promega (#J5021) was added and incubated for 10 min at room temperature. The resulting absorbance was recorded at a

(5) Brief pulses of high-level fluid shear stress enhance metastatic potential and rapidly alter the metabolism of cancer cellsCell ReportsFebruary 24, 2026Amanda N. Pope, Devon L. Moose, Guy O. Hudson, Hank R. Weresh, Marion R. Dykstra, Aabha Y. Joshi, Patrick Breheny, Eric B. Taylor, Michael D. Henryvalues. Lactate production The rate of lactate production was measured using Lactate-Glo assay kit (Promega, Cat#J5021). Our cancer cell lines were exposed to FSS or held in static conditions, washed with PBS, then immediately

(6) KRAS-dependent glycolytic reprogramming of endothelial cells in sporadic arteriovenous malformationsEMBO Molecular MedicineFebruary 18, 2026Ruilin Wu, Negar Khosraviani, Ann Mansur, Emilie Boudreau, Gabrielle E Largoza, Suejean Park, Dakota Gustafson, Sneha Raju, Crizza Ching, Amira Klip, et al.condition. Lactate-Glo™ assay Extracellular lactate was measured using the Lactate-Glo™ Assay Kit (Promega, Cat #: J5021) following the manufacturer’s instructions. Following serum/glucose starvation as described above for

(7) Differential roles of the type I and II secretion systems for the intracellular ABC141 Acinetobacter baumannii infection, which elicits an atypical hypoxia response in endothelial cellsPLOS PathogensFebruary 9, 2026Charline Debruyne, Landon Hodge, Karsten Hokamp, Anna S. Ershova, Carsten Kröger, Suzana P. Salcedolevels were determined with the Glucose-Glo Assay (Promega, J6021) and the Lactate-GloTAssay (Promega, J5021) following the manufacturer’s instructions. Briefly, after 24 h infection, cells were washed with cold

(8) Targeting metabolic vulnerabilities: REV-ERB agonist SR9009 potentiates sorafenib efficacy in liver cancerCell Death DiscoveryJanuary 19, 2026Silvia Sabbioni, Paola Guerriero, Ram C. Shankaraiah, Laura Masatti, Angelo Michilli, Cristian Bassi, Lucilla D’Abundo, Farzaneh Moshiri, Bruno De Siena, Edi Simoni, et al.associated with changes in cell membrane integrity and cellular ATP levels. The Lactate-Glo™ Assay (#J5021, Promega, Madison, WI, USA), a bioluminescent detection system that couples lactate oxidation and NADH

(9) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega, J5021). Thymocytes were isolated from untreated and TBI-treated mice and incubated in RPMI with 5 mM sodium

(10) Cooperative CCL2/CCR2 and HGF/MET signaling enhances breast cancer growth and invasion associated with metabolic reprogrammingCancer Biology & TherapyDecember 31, 2025Wei Fang, Yuuka Kozai, Diana S. Acevedo, Rebecca Brodine, Haasini S. Gorrepati, Nizhoni Arviso, Paige Cote, Alala Thompson, Zachary Gerdes, Ashley Espinoza, et al.instructions. Intracellular lactate levels were measured using the Lactate-Glow Assay (Promega, cat no.J5021), according to manufacturer’s instructions. Luciferase activity was measured using a Tecan Infinite

(11) Patient-derived colon epithelial organoids reveal lipid-related metabolic dysfunction in pediatric ulcerative colitisNat CommunDecember 10, 2025Babajide A. Ojo, Ying Zhu, Lyong Heo, Sejal R. Fox, Yanli Qiao, Amanda Waddell, Maria E. Moreno-Fernandez, Marielle Gibson, Tracy Tran, Ashley L. Dunn, et al.glucose and lactate were measured using the Glucose-Glo (Promega, #J6021) and Lactate-Glo (Promega, #J5021) assays, respectively. Samples were diluted 50X in ice-cold PBS, and the measurements continued according

(12) Small Extracellular Vesicles Secreted by Cisplatin-Resistant Neuroblastoma Cells Increase Lactate Secretion and Alter Metabolic Pathways in Primary Human Umbilical Vein Endothelial Cells (HUVECs)J Pers MedDecember 1, 2025Thomas Frawley, Lin Ma, Muhammad Zainul Arifin, Dan Wu, Alysia Scott, Brenton Cavanagh, Donal F. O'Shea, Vadim Zhernovkov, Mi Liu, Marco P. Monopoli, et al.1.53f51). 2.14. Lactate Secretion The lactate secretion assay utilised the Lactate-Glo™ Assay (Promega, Cat #J5021, Madison, WI, USA) according to the manufacturer’s guidelines. Conditioned media were collected from

(13) NADPH Oxidase Isoform NOX-2 Deficiency Affects Mitochondrial Oxygen Consumption and Metabolic FlexibilityRedox BiologyNovember 26, 2025Kannathasan Thetchinamoorthy, Diana Wierzbicka, Emilia Waraksa-Zasada, Michalina Kazek, Adrian Konopko, Mariusz Z. Ratajczak, Magdalena KuciaNox2-KO BM cells was measured using the Lactate-Glo® Assay (Promega, Madison, WI, USA, Catalog No. J5021) following the manufacturer's instructions. Control cells (untreated) and cells treated with H2O2 (10

(14) Defined metabolic states shape T cell fate and function across culture conditionsFrontiers in ImmunologyNovember 6, 2025Kayla Sylvester, Natasha Karassina, Anthony C. Lauer, Gediminas Vidugiris, Jolanta VidugirieneHandler (Beckman Coulter) and analyzed using Promega kits for glucose (Cat. #J6021), lactate (Cat. #J5021), and malate (Cat. #J8021). Extracellular metabolite concentrations were determined by comparing sample

(15) Adaptive Gene Expression Induced by a Combination of IL-1β and LPS in Primary Cultures of Mouse AstrocytesCellsNovember 5, 2025Thierry Coppola, Gwénola Poupon, Hélène Rangone, Stéphane Martin, Patricia Lebrunextracellular medium, we used the Lactate-Glo Assay from Promega, Charbonnières-les-Bains, France, (#J5021). Culture medium was collected at 0 and 5 days after treatment. 25 µL of extracellular medium was mixed

(16) Microbial metabolite indole-3-propionic acid drives mitochondrial respiration in CD4+ T cells to confer protection against intestinal inflammationNature MetabolismOctober 21, 2025Qing Li, Rodrigo de Oliveira Formiga, Virginie Puchois, Laura Creusot, Ahmad Haidar Ahmad, Salomé Amouyal, Márcio Augusto Campos-Ribeiro, Yining Zhao, Danielle M. M. Harris, Frederic Lasserre, et al.collected and analysed for lactate concentration using a commercially available assay kit (Promega, J5021), following the manufacturer’s protocol. MitoTracker Green/Deep Red and ROS production For mitochondria

(17) Nicotinamide phosphoribosyltransferase (Nampt) in Lateral Hypothalamus Maintains Skeletal Muscle Functions Through Lactate‐Mediated Calcium Signalling in Male MiceJournal of Cachexia, Sarcopenia and MuscleOctober 1, 2025Takahiro Eguchi, Keiko Kabetani, Naoki Itodescribed [10]. Intracellular lactate and serum insulin levels were measured using Lactate‐Glo (Promega, J5021) and insulin measurement kit (Takara, M1108), respectively. To measure the effect of formoterol, 0.2

(18) Reprogramming of cancer metabolism via photoresponsive nano-PROTAC enhances pyroptosis-mediated immunotherapySignal Transduction and Targeted TherapySeptember 26, 2025Byeongmin Park, Jiwoong Choi, Jae-Hyeon Lee, Yelee Kim, Woohyeong Lee, Ansoo Lee, In-Cheol Sun, Hong Yeol Yoon, Yongju Kim, Sun Hwa Kim, et al.above. Extracellular lactate in the culture medium was measured via a Lactate-Glo™ Assay Kit (Cat. No. J5021, Promega), whereas oxygen levels were assessed via CLSM imaging after staining with the oxygen indicator

(19) Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferationPLOS BiologySeptember 16, 2025Ana Luisa Dian, Lucilla Fabbri, Antoine Moya-Plana, Giuseppina Claps, Juliana C. Ferreira, Céline M. Labbé, Virginie Quidville, Sylvain Martineau, Dorothée Baille, Laetitia Besse, et al.measurement assay Lactate concentration in A375 cells was measured using the Lactate-Glo kit (Promega, #J5021) according to the manufacturer’s instructions. Cells were seeded in duplicates in six-well plates and

(20) Optineurin deficiency disrupts phosphorylated tau proteostasis and clusterin expression in human neuronsActa Neuropathologica CommunicationsSeptember 2, 2025Zachary M. Augur, Garrett M. Fogo, Mason R. Arbery, Andrew M. Stern, Courtney R. Benoit, Yi-Chen Hsieh, Tracy L. Young-Pearsediluted 1:25 in DPBS. Lactate content in samples was determined with the Lactate-Glo Assay (Promega Cat# J5021) according to manufacturer’s instructions. Luciferase signal was quantified by Infinite M Plex plate

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1/25/2024Rapid Assay From Promega for Detecting Lactate Secretion Kateryna MurlanovaWe measure cell culture lactate (secreted extracellular lactate) to assess energy metabolism changes, specifically glycolysis, in cultured immunomagnetically isolated astrocytes.

Lactate-Glo Assay

Lactate-Glo Assay from Promega

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Lactate-Glo Assay from Promega

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