Glucose-Glo Assay J6021 from Promega

Product Specs
ItemGlucose-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ6021
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Class I HDAC inhibition enhances the stem-like memory properties of CRISPR-engineered CAR T cells in neuroblastomaMol Ther OncolJune 18, 2026Dan Cappabianca, Mahmoud Toulany, Seth Zima, Amanda Shea, Jolanta Vidugiriene, Anthony Lauer, Kayla Sylvester, Varun Gnanasekar, Sean Foster, Rithvik Turaga, et al.glutamate levels were measured using Lactate-Glo, Malate-Glo, and Glutamine/Glutamate-Glo (cat. nos. J6021, J5021, JE9100, and J8021; Promega, Madison, WI), respectively. For each donor, inhibitor-treated samples

(2) A tissue-engineered human psoriatic skin model: targeting inflammation and glucose metabolism dysregulation in psoriasis using microneedle patchesFront Mol BiosciJune 9, 2026Yasmine Ruel, Fatma Moawad, Sergio Cortez Ghio, Davide Brambilla, Roxane Pouliotthe Human Insulin ELISA Kit (cat. KAQ1251, ThermoFisher Scientific) and the Glucose-Glo™ Assay (cat. J6021, Promega Corporation, Fitchburg, WI, USA), respectively. For each assay, a supernatant volume of 50

(3) Increased Glucose Availability Sensitizes Pancreatic Cancer to Macrophage-Targeting ImmunotherapiesCancer Res CommunJune 1, 2026Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud, Goutam Dey, Hallie J. Graor, Katie E. Blise, Dove Keith, Shamilene Sivagnanam, Shakti P. Pattanayak, et al.2-mm pieces, and subsequently homogenized. Glucose was quantified using a Promega Glucose-Glo assay (J6021). Flow cytometry Mice with confirmed tumors were first randomized to 7 days of D30 or standard water

(4) Combating VEGFA‐siRNA‐Induced Metabolic Reprogramming via Glucose Utilization DeprivationAdvanced ScienceJune 1, 2026Lulu Zheng, Shuai Guo, Yingjixing Luo, Pengfei Wu, Haiyin Yang, Yingqiu Xie, Yuchuan Fan, Qing Liu, Bo Hu, Jia Huang, et al.Oxygen Species (ROS) Assay Kit (S0033S) were purchased from Beyotime (China). Glucose Assay Reagent (J6021) was purchased from Promega (USA). 4.2 Animals BALB/c and BALB/c‐nu mice were obtained from SPF (Beijing)

(5) Metabolic assessment of iPSC-derived neurons under ketone-enriched condition: Ketone sensor development and BHB-driven metabolic adaptationiScienceMay 15, 2026Rohollah Nasiri, Farzaneh Fayazbakhsh, Reyhaneh Sanei, Tingting Wu, Nayere Taebnia, Rouhollah Habibey, Omid Fotouhi, John Cognetti, Volker M. Lauschke, Aman Russom, et al.Quant iT PicoGreen dsDNA Assay Kit,Thermo Fisher Scientific,Cat# P11496 Glucose Glo Assay Kit,Promega,Cat# J6021 Lactate Assay Kit,Promega,Cat# J5021 TaqMan Gene Expression Assays,Thermo Fisher Scientific, SLC16A1,Thermo

(6) Sex-specific systemic and brain metabolic responses to a standardized ketogenic diet in miceLab Anim (NY)May 12, 2026Charlotte Lorin, Giacomo Diaceri, Miguel Garcia, Emmanuelle Logette, Henry Markram, Daniel KellerLactate, glucose, glutamine and glutamate were measured using specific kits (lactate-J5021, Glucose-J6021, Glutamine/Glutamate-J8021, Promega). Metabolite concentrations were calculated from standard curves

(7) SPNS2 exports sphingosine-1-phosphate and imports glucoseNat CommunApril 20, 2026Cynthia Weigel, Md Lokman Hossen, Ryan D. R. Brown, Can E. Senkal, Christopher D. Green, Jason Newton, Sumit Saha, Elisa N. D. Palladino, Bin Ni, Francesco S. Celi, et al.levels in perfused tissues, feces, urine and lymph fluid were measured with the Glucose-Glo Assay (#J6021, Promega). 3–15 mg of tissue or feces were homogenized in 1 ml of 50 mM Tris (pH 7.5) pre-mixed with

(8) Taurine and betaine promote lactate clearance in canine hepatocytes by enhancing gluconeogenesisFront Vet SciApril 13, 2026Yan Ma, Jiaxi Li, Yunliang Li, Lei Lvon ice. Cellular glucose levels were quantified using the Glucose-Glo™ Assay Kit (Promega, Cat No. J6021) and phosphoenolpyruvate levels were quantified using the phosphoenolpyruvate Assay Kit (Coibo Cat No.

(9) Proteomic Profiling of Primary Hippocampal Neurons Reveals Noncanonical GFAP Expression and Metabolic Adaptations in Glia‐Free CulturePROTEOMICSApril 10, 2026Dominika Drulis‐Fajdasz, Kinga Gostomska‐Pampuch, Natalia Pudełko‐Malik, Mariusz Fleszar, Agnieszka Gizak, Jacek R. Wiśniewski, Dariusz Rakuslevels in culture media were measured using a modified protocol based on the Glucose‐Glo Assay Kit (J6021, Promega, USA). Reagents were thawed on ice (Luciferin Detection Solution at room temperature) and mixed

(10) Brief pulses of high-level fluid shear stress enhance metastatic potential and rapidly alter the metabolism of cancer cellsCell ReportsFebruary 24, 2026Amanda N. Pope, Devon L. Moose, Guy O. Hudson, Hank R. Weresh, Marion R. Dykstra, Aabha Y. Joshi, Patrick Breheny, Eric B. Taylor, Michael D. Henryas: Rateoflactateproduction=(lactateincellculture)/((cellnumberwell−1)×24×60×60)). Glucose uptake The rate of glucose uptake was measured using Glucose-Glo assay kit (Promega, Cat#J6021). Our cancer cell lines were exposed to FSS or held in static conditions, washed with PBS, then immediately

(11) Differential roles of the type I and II secretion systems for the intracellular ABC141 Acinetobacter baumannii infection, which elicits an atypical hypoxia response in endothelial cellsPLOS PathogensFebruary 9, 2026Charline Debruyne, Landon Hodge, Karsten Hokamp, Anna S. Ershova, Carsten Kröger, Suzana P. Salcedocells Intracellular glucose and lactate concentration levels were determined with the Glucose-Glo Assay (Promega, J6021) and the Lactate-GloTAssay (Promega, J5021) following the manufacturer’s instructions. Briefly, after

(12) Patient-derived colon epithelial organoids reveal lipid-related metabolic dysfunction in pediatric ulcerative colitisNat CommunDecember 10, 2025Babajide A. Ojo, Ying Zhu, Lyong Heo, Sejal R. Fox, Yanli Qiao, Amanda Waddell, Maria E. Moreno-Fernandez, Marielle Gibson, Tracy Tran, Ashley L. Dunn, et al.thawed on ice, and extracellular glucose and lactate were measured using the Glucose-Glo (Promega, #J6021) and Lactate-Glo (Promega, #J5021) assays, respectively. Samples were diluted 50X in ice-cold PBS, and

(13) Defined metabolic states shape T cell fate and function across culture conditionsFrontiers in ImmunologyNovember 6, 2025Kayla Sylvester, Natasha Karassina, Anthony C. Lauer, Gediminas Vidugiris, Jolanta Vidugirienean Echo Acoustic Liquid Handler (Beckman Coulter) and analyzed using Promega kits for glucose (Cat. #J6021), lactate (Cat. #J5021), and malate (Cat. #J8021). Extracellular metabolite concentrations were determined

(14) ER Stress Ire1‐Xbp1s Pathway Maintains Youthful Epidermal Basal Layer Through the Regulation of Cell ProliferationAging CellOctober 12, 2025Daniel Semmy, Kota Abe, Mizuki Honda, Hiroko Omori, Shohei Ogamino, Tobias Clausen Mercurio, Kyosuke Asakawa, Emi K. Nishimura, Shinya Oki, Yasuyuki Ohkawa, et al.and the supernatant was collected, followed by a glucose assay using the Glucose‐Glo Assay (Cat no. J6021, Promega Co. Ltd., Madison, WI USA). Total protein was measured from the sample diluted in 0.5% Triton

(15) Cysteinyl leukotriene receptor 1 regulates cellular glucose levels in human retinal cellsMol VisSeptember 14, 2025Andreas Koller, Susanne Maria Brunner, Julia Preishuber-Pflügl, Daniela Mayr, Christian Runge, Herbert Anton Reitsamer, Andrea Trostinstructions (G7570; Promega, Madison, WI). Intracellular glucose was measured with a Glucose-Glo Assay (J6021; Promega) following the manufacturer’s instructions. Enzyme-linked immunosorbent assay To measure

(16) Nintedanib Induces Mesenchymal-to-Epithelial Transition and Reduces Subretinal Fibrosis Through Metabolic ReprogrammingInternational journal of molecular sciencesJuly 24, 2025Hughes D, Prestle J, Zippel N, McFetridge S, Szczepan M, Neubauer H, Xu H, Chen M.above. Three assays measuring four metabolite assessments were performed, including Glucose-Glo (Cat. J6021, Promega, Seattle, WA, USA), Lactate-Glo (Cat. J5021, Promega, Seattle, WA, USA), and Glutamine/Glutamate-Glo

(17) Pharmacologic ascorbate resistant pancreatic cancer demonstrates enhanced metastatic potential☆Redox BiologyJuly 1, 2025Amanda Pope, Brianne O'Leary, Juan Du, Garry R. Buettner, Michael Henry, Joseph J. Cullencurve. Glucose uptake – The rate of glucose uptake was measured using Glucose-Glo assay kit (Promega, J6021) and protocol based on manufacturer's guidelines. Cells were plated at 2 x 104 cells well−1 in 200 μL

(18) Dietary lipid overload creates a suppressive environment that impedes the antiviral functions of NK cellsiScienceMay 16, 2025Simone Schimmer, Leonie Kerkmann, Nele Kahlert, Shahd al Jubeh, Tanja Werner, Carrie Corkish, Hannah Prendeville, David K. Finlay, Kathrin Sutter, Ulf Dittmer, et al.commercial assays,Critical commercial assays,Critical commercial assays ,, Glucose-Glo Assay,Promega,Cat# J6021 Luna Universal qPCR Master Mix,NEB,M3003LM eBioscience Foxp3/Transcription Factor Staining Buffer

(19) Tumor cell-derived ISG15 promotes fibroblast recruitment in oral squamous cell carcinoma via CD11a-dependent glycolytic reprogrammingOncogenesisMarch 11, 2025Wang SH, Chen YL, Huang SH, Fu YK, Lin SF, Jiang SS, Liu SC, Hsiao JR, Chang JY, Chen YW.using a fluorescent microscope. Lactate production and glucose uptake Quantification of glucose (J6021, Promega, Madison, WI, USA) and L-lactate (J5021, Promega) assay kits were used according to manufacturer’s

(20) HOTAIR Participation in Glycolysis and Glutaminolysis Through Lactate and Glutamate Production in Colorectal CancerCellsMarch 6, 2025Laura Cecilia Flores-García, Verónica García-Castillo, Eduardo Pérez-Toledo, Samuel Trujano-Camacho, Oliver Millán-Catalán, Eloy Andrés Pérez-Yepez, Jossimar Coronel-Hernández, Mauricio Rodríguez-Dorantes, Nadia Jacobo-Herrera, Carlos Pérez-PlasenciaExtracellular glucose concentration was determined by the Glucose-Glo™ Assay kit (Promega Corporation, Cat. J6021, Madison, WI, USA) as described by the manufacturer. Extracellular lactate levels were quantified using

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Glucose-Glo Assay

Glucose-Glo Assay from Promega

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Glucose-Glo Assay from Promega

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