MojoSort™ Human Pan Monocyte Isolation Kit 480059 from BioLegend

Product Specs
ItemMojoSort™ Human Pan Monocyte Isolation Kit
CompanyBioLegend
Price $249.00Supplier Page View Company Product Page
Catalog Number480059
Quantity20 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityHuman
Cell Type (standardized)Monocyte

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Harnessing extracellular vesicles for stabilized and functional IL-10 delivery in macrophage immunomodulationExtracellular VesicleJune 29, 2026Najla A. Saleh, Matthew A. Gagea, Xheneta Vitija, Sadhana Kilangodi, Ahmed A. Zarea, Tomas Janovic, Jens C. Schmidt, Cheri X. Deng, Masamitsu Kanadafrom the PBMCs by magnetic separation using the MojoSort™ Human Pan Monocyte Isolation Kit (BioLegend, 480059) following the manufacturer’s instruction. All cells were incubated at 37 °C in a 5 % CO2 atmosphere.

(2) USP1-mediated lipophagy-lipogenesis axis drives cholangiocarcinoma progression and immune evasionJ Immunother CancerJune 8, 2026Yananlan Chen, Xiao Xu, Shenye Shao, Jiawei Zhang, Ziyang Wang, Changan Chen, Wangjie Jiang, Jiang Chang, Ruixiang Chen, Tao Zhou, et al.density gradient centrifugation, followed by monocyte enrichment with the MojoSort Human Pan Monocyte Isolation Kit (#480059, BioLegend). Purified monocytes were subsequently cultured for 7 days in medium containing 100 ng/mL

(3) Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapyFront ImmunolApril 16, 2026Xuanyou Liu, Iris Kemler, David DingliCD14-positive cells prior to differentiation using the human pan monocyte isolation kit (Biolegend, Cat #480060) according to the manufacturer’s instructions. The resulting monocytes were cultured at 1×106 cells/ml

(4) Aroclor 1254 inhibits anti-inflammatory macrophage polarization through an AhR-dependent mechanismJournal of the Endocrine SocietyJanuary 7, 2026Riley M Behan-Bush, Elizabeth Kilburg, Jesse N Liszewski, Michael V Schrodt, Edward A Sander, Aloysius J Klingelhutz, James A Ankrummonocytes were isolated from PBMCs using the MojoSort Human Pan Monocyte Isolation Kit (BioLegend, Cat#: 480060), which is a magnetic bead-based negative selection isolation kit. PBMCs were first washed in 1× MojoSort

(5) Polychlorinated biphenyls induce immunometabolic switch of anti-inflammatory macrophages toward an inflammatory phenotypePNAS NexusMarch 27, 2025Riley M Behan-Bush, Michael V Schrodt, Elizabeth Kilburg, Jesse N Liszewski, Laura M Bitterlich, Karen English, Aloysius J Klingelhutz, James A Ankrumisolation, the monocytes were isolated using the MojoSort Human Pan Monocyte Isolation kit (BioLegend, cat#: 480060), a magnetic bead-based negative selection kit. The PBMCs were first washed with 1 × MojoSort Buffer

(6) Conventional and antibody-enhanced DENV infection of human macrophages induces differential immunotranscriptomic profilesJournal of VirologyMarch 18, 2025Céline S. C. Hardy, Adam D. Wegman, Mitchell J. Waldran, Gary C. Chan, Adam T. Waickmanused to isolate monocytes by negative selection according to the manufacture’s protocol (Biolegend, #480059) and cells were resuspended in RPMI medium supplemented with 100 ng/mL M-CSF (Peprotech, # 300-25),

(7) CD4+ T cell–innate immune crosstalk is critical during Staphylococcus aureus craniotomy infectionJCI InsightFebruary 24, 2025Gunjan Kak, Zachary Van Roy, Rachel W. Fallet, Lee E. Korshoj, Tammy KielianAB_2562053), and autologous monocytes were enriched using a MojoSort human pan monocyte isolation kit (480060, BioLegend). To assess how human CD4+ T cells affect monocyte bactericidal activity, gentamicin protection

(8) Scavenger Receptor CD36 in Tumor-Associated Macrophages Promotes Cancer Progression by Dampening Type I Interferon SignalingCancer ResearchFebruary 1, 2025Ziyan Xu, Alexandra Kuhlmann-Hogan, Shihao Xu, Hubert Tseng, Dan Chen, Shirong Tan, Ming Sun, Victoria Tripple, Marcus Bosenberg, Kathryn Miller-Jensen, et al.cell separation, then enriched for monocytes using MojoSort™ Human Pan Monocyte Isolation Kit (Biolegend, # 480059). Purified monocytes were differentiated to macrophages in culture with 100 ng/mL M-CSF (Biolegend #

(9) Scavenger Receptor CD36 in Tumor-Associated Macrophages Promotes Cancer Progression by Dampening Type I Interferon SignalingCancer ResFebruary 1, 2025Ziyan Xu, Alexandra Kuhlmann-Hogan, Shihao Xu, Hubert Tseng, Dan Chen, Shirong Tan, Ming Sun, Victoria Tripple, Marcus Bosenberg, Kathryn Miller-Jensen, et al.cell separation, then enriched for monocytes using MojoSort™ Human Pan Monocyte Isolation Kit (Biolegend, # 480059). Purified monocytes were differentiated to macrophages in culture with 100 ng/mL M-CSF (Biolegend #

(10) Harnessing Extracellular Vesicles for Stabilized and Functional IL-10 Delivery in Macrophage ImmunomodulationbioRxivJanuary 18, 2025Najla A. Saleh, Matthew A. Gagea, Xheneta Vitija, Tomas Janovic, Jens C. Schmidt, Cheri X. Deng, Masamitsu Kanadafrom the PBMCs by magnetic separation using the MojoSort™ Human Pan Monocyte Isolation Kit (BioLegend, 480059) following the manufacturer’s instruction. All cells were incubated at 37°C in a 5% CO2 atmosphere.

(11) Rapid unleashing of macrophage efferocytic capacity via transcriptional pause releaseNatureApril 1, 2024Tufan T, Comertpay G, Villani A, Nelson GM, Terekhova M, Kelley S, Zakharov P, Ellison RM, Shpynov O, Raymond M, et al.macrophage differentiation. Monocytes were separated from thawed PBMCs by negative selection (BioLegend, 480060) and plated on non-tissue culture coated dishes in RPMI containing 10% FBS, l-glutamine, penicillin-streptomycin

(12) Human CD4+CD25+ T cells expressing a chimeric antigen receptor (CAR) against aberrant superoxide dismutase-1 (SOD1) triggers antigen-specific immunomodulationCytotherapyFebruary 1, 2024David J. Graber, W. James Cook, Marie-Louise Sentman, Joana M. Murad-Mabaera, Charles L. Sentmanlineage cells were isolated from PBMCs using MojoSort™ Human Pan Monocyte Isolation Kit (Biolegend 480060). Cells were plated at 50,000 cells/0.1mL/well in XVIVO-15 media with 10% human sera in either 96-well

(13) Human placental cells are resistant to SARS-CoV-2 infection and replicationWellcome open researchJanuary 1, 2024Yoshida N, Thomas JR, Appios A, Brember MP, Aye ILMH, Edgar JR, Firth AE, Chung BYW, McGovern N, Stewart H.MojoSort™ Human Pan Monocyte Isolation Kit performed according to the manufacturer’s instructions (Biolegend, 480060) and cultured in media supplemented with M-CSF (Gibco, PHC9501) with further 50 % media changes at 3

(14) DENV-specific IgA contributes protective and non-pathologic function during antibody-dependent enhancement of DENV infectionPLOS PathogensAugust 28, 2023Adam D. Wegman, Mitchell J. Waldran, Lauren E. Bahr, Joseph Q. Lu, Kristen E. Baxter, Stephen J. Thomas, Adam T. Waickmanmonocytes were isolated by negative selection according to the manufacture’s protocol (Biolegend, #480059) and were resuspended in RPMI supplemented to with 100ng/mL M-CSF (Peprotech, # 300–25), 10% (v/v) FBS

(15) Macrophage Sprouty4 deficiency diminishes sepsis-induced acute lung injury in miceRedox biologyDecember 1, 2022Chen R, Cao C, Liu H, Jiang W, Pan R, He H, Ding K, Meng Q.from R&D Systems, Inc. (Minneapolis, MN, Canada). MojoSort™ Human Pan Monocyte Isolation Kit (CAT#: 480060) was purchased from BioLegend (USA). Human Macrophage Colony-Stimulating Factor (M-CSF) Recombinant

(16) Morphine disrupts macrophage functions even during HIV infectionJournal of leukocyte biologyNovember 1, 2022Barbaro JM, Jaureguiberry-Bravo M, Sidoli S, Berman JW.negatively isolated monocytes from collected PBMC by MojoSort Pan Monocyte Isolation (Biolegend Cat# 480060, San Diego, CA) according to manufacturer’s protocols for phagocytosis and ROS experiments. In both

(17) Methamphetamine Dysregulates Macrophage Functions and Autophagy to Mediate HIV NeuropathogenesisBiomedicinesMay 27, 2022Barbaro JM, Sidoli S, Cuervo AM, Berman JW.were negatively isolated from PBMC using the MojoSort Pan Monocyte Isolation Kit (Biolegend Cat. No.: 480060, San Diego, CA, USA) according to the manufacturer’s protocols. These are commonly used methods to generate

(18) HIV Increases the Inhibitory Impact of Morphine and Antiretrovirals on Autophagy in Primary Human Macrophages: Contributions to NeuropathogenesisCellsAugust 24, 2021Barbaro JM, Cuervo AM, Berman JW.were isolated by negative selection using the MojoSort Pan Monocyte Isolation Kit (Biolegend Cat. No.: 480060, San Diego, CA, USA), and 100,000 monocytes/well were plated on PDL-coated round glass coverslips (Corning)

(19) Manganese salts function as potent adjuvantsCellular & molecular immunologyMay 1, 2021Zhang R, Wang C, Guan Y, Wei X, Sha M, Yi M, Jing M, Lv M, Guo W, Xu J, et al.monocytes were isolated from human PBMCs with a MojoSort™ Human Pan Monocyte Isolation Kit (Biolegend, Cat# 480060) and cultured in RPMI-1640 (Gibco) medium supplemented with 10% FBS (Gibco), 100 ng/ml hIL-4 (Genscript),

(20) Caspase-11-Gasdermin D-Mediated Pyroptosis Is Involved in the Pathogenesis of AtherosclerosisFrontiers in pharmacologyJanuary 1, 2021Jiang M, Sun X, Liu S, Tang Y, Shi Y, Bai Y, Wang Y, Yang Q, Yang Q, Jiang W, et al.monocytes were isolated from the human PBMCs using the MojoSort™ Human Pan Monocyte Isolation Kit (480060, BioLegend, San Diego, CA, United States) according to the instructions. Isolation of Mouse Peritoneal Macrophages

Be the First to Write a Review!

MojoSort™ Human Pan Monocyte Isolation Kit from BioLegend

Description

BioLegend

Citations (22)

Reviews

MojoSort™ Human Pan Monocyte Isolation Kit from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map