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ELISpot Flex: Mouse IgG (HRP)
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ELISpot Flex: Mouse IgG (HRP)
ELISpot Flex: Mouse IgG (HRP) from Mabtech AB
Be the first to write a review!
Citations:
(17)
Figures:
(4)
Supplier Page
Supplier Page from
Mabtech AB for
ELISpot Flex: Mouse IgG (HRP)
Description
Mouse IgG ELISpot Flex enables enumeration of B cells secreting total mouse IgG as well as antigen-specific mouse IgG. It contains capture and detection antibodies, Streptavidin-HRP, and polyclonal activators
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ELISpot Flex: Mouse IgG (HRP)
Company
Mabtech AB
Price
$505.00
Supplier Page
View Company Product Page
Catalog Number
3825-2H
Quantity
for 4 plate
Applications
ELISpot
Reactivity
mouse
Conjugate/Tag/Label
HRP
Format
without plates
Detection Target
IgG
NCBI Full Gene Name
immunoglobulin heavy constant gamma 2A, Immunoglobulin heavy constant gamma 3, immunoglobulin heavy constant gamma 2C, immunoglobulin heavy constant gamma 2B, immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Gene Aliases
IgG2b, VH7183, Igh-1a, Igh-1b, IgG1, IgG3, gamma2b, 1810060O09Rik, Igh-4, Igh-3, Igh-1
Type
Streptavidin-HRP
Components
Capture Ab (anti-IgG),Biotinylated detection Ab (anti-IgG),Streptavidin-HRP,R848,Recombinant mouse IL-2
Add to Compare List
Mabtech AB
Box 1233
Nacka Strand
,
SE-131 28
Sweden
Phone
: +46 8 716 27 00
+46 8 716 27 01
Company Profile
Website:
http://www.mabtech.com
Citations (17)
(1)
Foot-and-mouth disease virus-like particle mRNA vaccine induces strong humoral and cellular immunity
Appl Microbiol Biotechnol
April 6, 2026
Haiyun Liu, Yun Zhang, Suyu Mu, Fengping Hou, LiXin Jiang, Zi Wang, Hu Dong, Muhammad Muntazir Mehdi, Huichen Guo, Shiqi Sun
Biotinylated anti-IgG antibody was added, followed by a streptavidin-peroxidase conjugation reaction (
Mabtech
,
3825-2H
). Substrate solution was added for spot development, and color development was stopped by thorough rinsing
(2)
Single-B cell analysis correlates high-lactate secretion with stress and increased apoptosis
Scientific reports
April 12, 2024
Bucheli OTM, Rodrigues D, Portmann K, Linder A, Thoma M, Halin C, Eyer K.
individually.
ELISpot
The number of B cells secreting IgG was quantified using a
mouse
ELISpot
kit (
Mabtech
,
3825-2H
). The isolated cells were counted using the
Countess Automated Cell Counter
II (
ThermoFisher
) and re-suspended
(3)
Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells
Nature biotechnology
September 1, 2023
Oh S, Mao X, Manfredo-Vieira S, Lee J, Patel D, Choi EJ, Alvarado A, Cottman-Thomas E, Maseda D, Tsao PY,
et al
.
cells in the
spleen
of
MuSK
-immunized
mice
were conducted using Mouse IgG ELISpotbasic kit (
Mabtech
,
3825-2H
) according to the manufacturer’s protocol. Briefly, splenocytes were prestimulated with a mixture of
(4)
An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission
bioRxiv : the preprint server for biology
November 24, 2022
Li W, Wang T, Rajendrakumar AM, Acharya G, Miao Z, Varghese BP, Yu H, Dhakal B, LeRoith T, Tuo W,
et al
.
signals. For measuring S-specific antibody-secretin cells (ASCs) in
bone
marrow, an
ELISpot
kit (
MabTech
, Cat#
3825–2H
) was used. The 96-well
ELISpot
plates (
MabTech
, Cat#
3654-TP-10
) were pre-wetted with 35% ethanol and
(5)
Intranasal nanoemulsion adjuvanted S-2P vaccine demonstrates protection in hamsters and induces systemic, cell-mediated and mucosal immunity in mice
PloS one
January 1, 2022
Ganesan S, Acosta H, Brigolin C, Orange K, Trabbic K, Chen C, Lien CE, Lin YJ, Lin MY, Chuang YS,
et al
.
PVDF
ELISpot
filter plates coated with anti-mouse IgG or IgA capture antibody (
Mabtech
, Cat# BASIC
3825-2H
and BASIC 3835-2H). The plates were incubated at 37°C for 24 hours, following which the cells were stained
(6)
C9orf72 deficiency promotes microglial-mediated synaptic loss in aging and amyloid accumulation
Neuron
July 21, 2021
Lall D, Lorenzini I, Mota TA, Bell S, Mahan TE, Ulrich JD, Davtyan H, Rexach JE, Muhammad AKMG, Shelest O,
et al
.
GolgiStain™ Kit,"FD Neuro Technologies, Inc.",Cat #PK401 Mouse IgG
ELISpot
kit (HRP),
Mabtech
,Cat #
3825–2H
Mouse
IFNg ELISPOT Set,
BD Biosciences
,Cat #
551083
Neural Tissue dissociation kit,
Miltenyi Biotech
,Cat
(7)
Microbiota-derived butyrate limits the autoimmune response by promoting the differentiation of follicular regulatory T cells
EBioMedicine
August 1, 2020
Daisuke Takahashi et al.
Enzyme-Linked immunospot (ELISpot
) CII-specific antibody-forming cells were detected by
ELISpot
using a Mouse IgG ELISpotBASIC HRP Kit (Cat. #
3825–2H
;
Mabtech
, Nacka Strand, Sweden) according to the manufacturer's instructions. Briefly, a MultiScreenHTS
(8)
Donor-specific chimeric antigen receptor Tregs limit rejection in naive but not sensitized allograft recipients
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
June 1, 2020
Sicard A, Lamarche C, Speck M, Wong M, Rosado-Sánchez I, Blois M, Glaichenhaus N, Mojibian M, Levings MK.
(9)
Malt1 Protease Deficiency in Mice Disrupts Immune Homeostasis at Environmental Barriers and Drives Systemic T Cell–Mediated Autoimmunity
The Journal of Immunology
December 1, 2019
Kea Martin et al.
cell strainer. IgG- and IgE-secreting cells were detected using the Mouse IgG
ELISpot
BASIC Kit (no.
3825-2H
;
Mabtech
) following the manufacturer’s protocol. For detection of IgE-secreting cells, the anti-IgG
(10)
Boosting subdominant neutralizing antibody responses with a computationally designed epitope-focused immunogen
PLOS Biology
February 21, 2019
Fabian Sesterhenn et al.
Data.
ELISpot
B cell ELISpot
assays
were performed using the Mouse IgG
ELISpot
HRP kit (
Mabtech
, #
38252H
) according to the manufacturer’s instructions. Briefly,
mouse
spleens were isolated and pressed through
(11)
Immunological responses following administration of a genotype 1a/1b/2/3a quadrivalent HCV VLP vaccine
Scientific reports
April 24, 2018
Christiansen D, Earnest-Silveira L, Chua B, Meuleman P, Boo I, Grubor-Bauk B, Jackson DC, Keck ZY, Foung SKH, Drummer HE,
et al
.
(12)
Immunization Elicits Antigen-Specific Antibody Sequestration in Dorsal Root Ganglia Sensory Neurons
Frontiers in immunology
January 1, 2018
Gunasekaran M, Chatterjee PK, Shih A, Imperato GH, Addorisio M, Kumar G, Lee A, Graf JF, Meyer D, Marino M,
et al
.
(13)
In vivo dendritic cell targeting cellular vaccine induces CD4+ Tfh cell-dependent antibody against influenza virus
Scientific Reports
December 1, 2016
Satoru Yamasaki, Kanako Shimizu, Kohei Kometani, Maki Sakurai, Masami Kawamura, Shin-ichiro Fujii
(14)
CEACAM1 mediates B cell aggregation in central nervous system autoimmunity
Scientific reports
July 20, 2016
Rovituso DM, Scheffler L, Wunsch M, Kleinschnitz C, Dörck S, Ulzheimer J, Bayas A, Steinman L, Ergün S, Kuerten S.
(15)
Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration
Scientific reports
July 14, 2016
Li QH, Jin G, Wang JY, Li HN, Liu H, Chang XY, Wang FX, Liu SL.
(16)
Lethal Coinfection of Influenza Virus and Streptococcus pneumoniae Lowers Antibody Response to Influenza Virus in Lung and Reduces Numbers of Germinal Center B Cells, T Follicular Helper Cells, and Plasma Cells in Mediastinal Lymph Node
Journal of virology
February 15, 2015
Yuet Wu, Wenwei Tu, Kwok-Tai Lam, Kin-Hung Chow, Pak-Leung Ho, Yi Guan, Joseph S Malik Peiris, Yu-Lung Lau
(17)
B cells promote insulin resistance through modulation of T cells and production of pathogenic IgG antibodies
Nature medicine
May 1, 2011
Winer DA, Winer S, Shen L, Wadia PP, Yantha J, Paltser G, Tsui H, Wu P, Davidson MG, Alonso MN,
et al
.
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ELISpot Flex: Mouse IgG (HRP) from Mabtech AB
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$505.00
Product Specs
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