ELISpot Flex: Human IgG (ALP) 3850-2A from Mabtech AB

Product Specs
ItemELISpot Flex: Human IgG (ALP)
CompanyMabtech AB
Price $505.00Supplier Page View Company Product Page
Catalog Number3850-2A
Quantityfor 4 plate
ApplicationsELISpot
ReactivityHuman,Non-human primates
Detection TargetIgG
Conjugate/Tag/LabelALP
NCBI Full Gene Nameimmunoglobulin heavy constant gamma 2 (G2m marker), immunoglobulin heavy constant gamma 3 (G3m marker), immunoglobulin heavy constant gamma 1 (G1m marker)
NCBI Gene AliasesIgG3
TypeStreptavidin-ALP
Formatwithout plates
ComponentsCapture mAbs (MT91/145),Biotinylated detection mAbs (MT78/145),Streptavidin-ALP,R848,Recombinant human IL-2

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) Robust effector memory features of human T-bethi B cells induced by repeated mRNA vaccinationiScienceFebruary 20, 2026Jeongsoo Lee, Seunghwan Son, Youseung Chung, Sung-Dong Cho, Ji-Soo Kwon, Seongman Bae, Joon Seok, Baekgyu Choi, Inkyung Jung, Ji Eun Oh, et al.Amplification Kit, 16 rxns",10X Genomics,Cat# PN-1000253 ELISpot Flex: Human IgG (ALP),MABTECH,Cat# 3850-2A SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Kit,GenScript,Cat# L00847-A SARS-CoV-2 Spike

(2) Integrating Single-Cell Biophysical and Transcriptomic Features to Resolve Functional Heterogeneity in Mantle Cell LymphomabioRxivMay 28, 2025Ye Zhang, Lydie Debaize, Adam Langenbucher, Jenalyn Weekes, Ioulia Vogiatzi, Teemu P. Miettinen, Mingzeng Zhang, Emily Sumpena, Huiyun Liu, Sarah M. Duquette, et al.and the production of total IgG at day 7 was measured using Human IgG ELIspot Basic (Mabtech, Inc. # 3850–2A). On days 7 and 10 to 14, the cells were sorted on a FACSAria II SORP fluorescence-activated cell sorter

(3) Immunogenicity and safety of an Entamoeba histolytica adjuvanted protein vaccine candidate (LecA+GLA-3M-052 liposomes) in rhesus macaquesHuman Vaccines & ImmunotherapeuticsDecember 31, 2024Mayuresh M. Abhyankar, Feifan Xu, Deborah Chavez, Anna Goodroe, Elda Mendoza, Christopher Chen, Dhiraj K. Singh, Fernando Varnador, Sandra J. Sivananthan, Robert Kinsey, et al.also quantitated by coating separate sets of plates with 15 μg/mL anti-human/monkey IgG (Mabtech, Cat# 3850-2A) or anti-human/monkey IgA (Mabtech, Cat# 3860M-2A). Week 14 PBMCs were activated using the B-Poly-S

(4) Enhanced Immune Response Against Echinococcus Granulosus Through a CTLA-4/B7 Affinity-Based VaccineVaccines (Basel)December 20, 2024Yuejie Zhu, Yueyue He, Ziyue Yin, Na Chen, Xingxing Qi, Jianbing Ding, Yujiao Li, Fengbo Zhangantibody-secreting cells (ASCs) were detected at the conclusion of the procedure. The ELISpot flex-human IgG (ALP) Kit (3850-2A, Mabtech, Stockholm, Sweden) was used for the ELISPOT experiment. 2.13. In Vivo Experiment In this

(5) Increased Natural Killer (NK)-cell cytotoxicity and Trypanosoma cruzi-specific memory B cells in subjects with discordant serology for Chagas diseaseBiochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseAugust 1, 2024María J. Elias, Gonzalo Cesar, María B. Caputo, Ana M. De Rissio, María G. Alvarez, Bruno Lococo, María A. Natale, Constanza López Albizu, Ariel Podhorzer, Cecilia Parodi, et al.antibody-secreting cells (ASCs) and memory B cells were conducted using the human IgG ELISPOT Basic kit (cat# 3850–2A, Mabtech, Sweden) according to the manufacturer’s recommendations and as previously reported [22]. Sterile

(6) Evaluation of an RBD-nucleocapsid fusion protein as a booster candidate for COVID-19 vaccineiScienceJuly 19, 2024Bruno Vinicius Santos Valiate, Julia Teixeira de Castro, Tomás Gazzinelli Marçal, Luis Adan Flores Andrade, Livia Isabela de Oliveira, Gabriela Barbi Freire Maia, Lídia Paula Faustino, Natalia S. Hojo-Souza, Marconi Augusto Aguiar Dos Reis, Flávia Fonseca Bagno, et al.assays,Critical commercial assays,Critical commercial assays ,, Human IgG ELISpotbasic kit,Mabtech,3850-2A OptEIA Human IFN-gamma,BD,555142 Mouse IFN-gamma DuoSet ELISA,R&D Systems,DY485 ,, Experimental models

(7) MVA-based vaccine candidates encoding the native or prefusion-stabilized SARS-CoV-2 spike reveal differential immunogenicity in humansnpj VaccinesJanuary 26, 2024Leonie Mayer, Leonie M. Weskamm, Anahita Fathi, Maya Kono, Jasmin Heidepriem, Verena Krähling, Sibylle C. Mellinghoff, My Linh Ly, Monika Friedrich, Svenja Hardtke, et al.S2 subunit (S1: 0.1 µg/ml, S2: 0.2 µg/ml; SinoBiological) and anti-IgG detection antibody (1 µg/ml; #3850-2A; Mabtech) were added to detect antigen-specific and total IgG-secreting B cells, respectively. For spot

(8) Antibody and memory B cell responses to the dengue virus NS1 antigen in individuals with varying severity of past infectionImmunologySeptember 1, 2023Shyrar Tanussiya Ramu, Madushika Dissanayake, Chandima Jeewandara, Farha Bary, Michael Harvie, Laksiri Gomes, Ayesha Wijesinghe, Dinuka Ariyaratne, Graham S. Ogg, Gathsaurie Neelika Malavige50,000 cells/well were added to the positive control wells. A Human IgG ELISpot BASIC kit (Mabtech 3850‐2A) was used according to the manufacturer's guidelines to quantify IgG‐secreting cells specific to DENV

(9) Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis SystemVaccinesMarch 26, 2023Tsai DY, Wang CH, Schiro PG, Chen N, Tseng JY.at a density of 1 × 106 cells/mL and stimulated with R848 (1 μg/mL; MabTech, Human IgG ELISpot kit, 3850-2A), IL-2 (10 ng/mL; MabTech, Nacka Strand, Sweden, Human IgG ELISpot kit, 3850-2A), IL-21 (100 ng/mL;

(10) Immune responses following the first dose of the Sputnik V (Gam-COVID-Vac)Scientific ReportsDecember 1, 2022Chandima Jeewandara et al.added. 50,000 cells/well were added to the positive control wells. A Human IgG ELISpot kit (Mabtech 3850-2A) was used according to the manufacturer’s instructions to quantify IgG-secreting cells specific to SARS-COV2

(11) Immune responses to Sinopharm/BBIBP‐CorV in individuals in Sri LankaImmunologyOctober 1, 2022Chandima Jeewandara, Inoka Sepali Aberathna, Pradeep Darshana Pushpakumara, Achala Kamaladasa, Dinuka Guruge, Ayesha Wijesinghe, Banuri Gunasekera, Shyrar Tanussiya, Heshan Kuruppu, Thushali Ranasinghe, et al.Fifty thousand cells/well were added to the positive control wells. A human IgG ELISpot kit (Mabtech 3850‐2A) was used according to the manufacturer's instructions to quantify IgG‐secreting cells specific to SARS‐COV2

(12) Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccineCell reports. MedicineJuly 19, 2022Weskamm LM, Fathi A, Raadsen MP, Mykytyn AZ, Koch T, Spohn M, Friedrich M, MVA-MERS-S Study Group, Haagmans BL, Becker S, et al.well Enzymatic ELISpot Kit,ImmunoSpot,Cat#hIFNg-3M/5 Human IgG ELISpot BASIC kit (ALP),Mabtech,Cat#3850-2A ,, Software and algorithms,Software and algorithms,Software and algorithms ,, FlowJo v10.8.0,"FlowJo,

(13) Persistence of immune responses to the Sinopharm/BBIBP‐CorV vaccineImmunity, Inflammation and DiseaseJune 1, 2022Chandima Jeewandara et al.total of 50,000 cells/well were added to the positive control wells. A Human IgG ELISpot kit (Mabtech 3850‐2A) was used according to the manufacturer's instructions to quantify IgG‐secreting cells specific to SARS‐COV2

(14) Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B CellsFrontiers in immunologyJanuary 1, 2019Zimmermann M, Rose N, Lindner JM, Kim H, Gonçalves AR, Callegari I, Syedbasha M, Kaufmann L, Egli A, Lindberg RLP, et al.HA-Specific, IgG-Secreting Human B Cells 96-well plates (Human IgG B cell ELISpot kit (Mabtech, Sweden, Code: 3850-2A) were coated overnight at 4°C with hemagglutinin (Sino Biological, Influenza A H1N1 (A/California/04/2009) hemagglutinin (HA) Protein (His Tag

(15) Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infectionNature communicationsAugust 20, 2018Song P, Zheng N, Liu Y, Tian C, Wu X, Ma X, Chen D, Zou X, Wang G, Wang H, et al.

(16) Persistence of memory B-cell and T-cell responses to the quadrivalent HPV vaccine in HIV-infected childrenAIDS (London, England)April 24, 2018Weinberg A, Huang S, Moscicki AB, Saah A, Levin MJ, IMPAACT P1085 Protocol Team..

(17) Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis PatientsJournal of immunology (Baltimore, Md. : 1950)February 15, 2017Bankó Z, Pozsgay J, Szili D, Tóth M, Gáti T, Nagy G, Rojkovich B, Sármay G.

(18) DNA-MVA-protein vaccination of rhesus macaques induces HIV-specific immunity in mucosal-associated lymph nodes and functional antibodiesVaccineFebruary 7, 2017Chege GK, Burgers WA, Müller TL, Gray CM, Shephard EG, Barnett SW, Ferrari G, Montefiori D, Williamson C, Williamson AL.

(19) Comparative phenotypical analysis of B cells in fresh and cryopreserved mononuclear cells from blood and tissue of rhesus macaquesJournal of immunological methodsJune 1, 2016Klippert A, Neumann B, Stahl-Hennig C.

(20) The forkhead transcription factor FOXP1 represses human plasma cell differentiationBloodOctober 29, 2015van Keimpema M, Grüneberg LJ, Mokry M, van Boxtel R, van Zelm MC, Coffer P, Pals ST, Spaargaren M.

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ELISpot Flex: Human IgG (ALP)

ELISpot Flex: Human IgG (ALP) from Mabtech AB

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Mabtech AB

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ELISpot Flex: Human IgG (ALP) from Mabtech AB

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