ChromaFlash One Step ChIP Kit P-2025 from EpiGentek

Product Specs
ItemChromaFlash One Step ChIP Kit
CompanyEpiGentek
Price Supplier Page View Company Product Page
Catalog NumberP-2025
Quantity48 Reactions, 96 Reactions
Sample TypeChromatin
Research AreaChromatin & Transcription

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956

Email: info@epigentek.com
sales@epigentek.com
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support@epigentek.com

Company Profile

Website: www.epigentek.com

(1) HELZ is a RNA-DNA helicase that resolves R loops to facilitate homologous recombination repairNat CommunJuly 23, 2026Ramona Haji-Seyed-Javadi, Allyson E. Koyen, Sandip K. Rath, Bo Wu, Matthew Z. Madden, Yingzi Hou, Priya Kapoor-Vazirani, Roshika Roshika, Meili Aiello, Nho Cong Luong, et al.analysis by FlowJo software. ChIP assay ChIP assay was performed using ChromaFlashTM One-Step ChIP Kit (P-2025, Epigentek, Farmingdale, NY, USA) according to the manufacturer’s instructions. Briefly, DIvA cells

(2) TIGAR deficiency enhances cardiac resilience through epigenetic programming of Parkin expressionJCI InsightApril 22, 2026Yan Tang, Stanislovas S. Jankauskas, Li Liu, Xujun Wang, Alus M. Xiaoli, Fajun Yang, Gaetano Santulli, Daorong Feng, Jeffrey E. Pessin1,000 bp. Chromatin immunoprecipitation (ChIP) was performed using the ChromaFlash One-Step ChIP Kit (P-2025, EpigenTek) following the manufacturer’s instructions. DNA-protein complexes were immunoprecipitated

(3) MRE11 deacetylation by SIRT2 promotes DNA binding to facilitate DNA end resection and ATM-dependent signalingJournal of Clinical InvestigationMarch 2, 2026Fatmata Sesay, Hui Zhang, Priya Kapoor-Vazirani, Andrew T. Jung, Mark E. Essien, Amanda J. Bastien, Nho C. Luong, Xu Liu, PamelaSara E. Head, Duc M. Duong, et al.(MilliporeSigma, T6074) antibodies. ChIP assay. ChIP was performed using the ChromaFlash One-Step ChIP Kit (P-2025, Epigentek) following the manufacturer’s protocol. Briefly, Fok1 cells were transfected with the SFB-MRE11

(4) MRE11 deacetylation by SIRT2 promotes DNA binding to facilitate DNA end resection and ATM-dependent signalingJ Clin InvestJanuary 8, 2026Fatmata Sesay, Hui Zhang, Priya Kapoor-Vazirani, Andrew T. Jung, Mark E. Essien, Amanda J. Bastien, Nho C. Luong, Xu Liu, PamelaSara E. Head, Duc M. Duong, et al.(MilliporeSigma, T6074) antibodies. ChIP assay. ChIP was performed using the ChromaFlash One-Step ChIP Kit (P-2025, Epigentek) following the manufacturer’s protocol. Briefly, Fok1 cells were transfected with the SFB-MRE11

(5) NEK7 induces lactylation in Alzheimer's disease to promote pyroptosis in BV-2 cellsMolecular brainNovember 19, 2024Cheng J, Zhao H.polymerase II) and a negative control non-immune IgG were used to validate the efficacy of the kit reagents (P-2025-48, Epigentek Group). The immunoprecipitated DNA was then purified, released, and eluted. The eluted

(6) DNA-PK is activated by SIRT2 deacetylation to promote DNA double-strand break repair by non-homologous end joiningNucleic acids researchAugust 25, 2023Head PE, Kapoor-Vazirani P, Nagaraju GP, Zhang H, Rath SK, Luong NC, Haji-Seyed-Javadi R, Sesay F, Wang SY, Duong DM, et al.immunoprecipitation (ChIP) ChIP assay was performed using ChromaFlash One-Step ChIP Kit (Epigentek, P-2025) according to the manufacturer's instruction. Briefly, Fok1-expressing U2OS-265 Fok1 cells were fixed

(7) SAMHD1 deacetylation by SIRT1 promotes DNA end resection by facilitating DNA binding at double-strand breaksNature communicationsNovember 7, 2022Kapoor-Vazirani P, Rath SK, Liu X, Shu Z, Bowen NE, Chen Y, Haji-Seyed-Javadi R, Daddacha W, Minten EV, Danelia D, et al.independent experiments. ChIP assay ChIP assay was performed using ChromaFlashTM One-Step ChIP Kit (P-2025, Epigentek, Farmingdale, NY, USA) according to the manufacture’s instruction. Briefly, DIvA cells were

(8) Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasisJournal of cellular and molecular medicineApril 1, 2020Gao L, Dou J, Zhang B, Zeng J, Cheng Q, Lei L, Tan L, Zeng Q, Ding S, Guo A, et al.IgG was used as a negative control demonstrate the efficacy of the kit reagents (Epigentek Group Inc, P‐2025‐48). The immunoprecipitated DNA was eluted and examined for fold‐enrichment according to the methods

(9) HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5pCell Death & DiseaseMarch 1, 2020Yufan Zhou, Yun Huang, Kuan Hu, Zeyu Zhang, Jiajin Yang, Zhiming WangII) and a negative control nonimmune IgG were used to demonstrate the efficacy of the kit reagents (P-2025-48, Epigentek Group Inc., NY, USA). The immunoprecipitated DNA was subsequently cleaned, and eluted.

(10) NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-κB signalingCell Death & DiseaseDecember 2, 2019Xueliang Chen, Ganglei Liu, Yuanyuan Yuan, Guotao Wu, Shalong Wang, Lianwen Yuannon-immune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group, NY, USA, P-2025-48). The immunoprecipitated DNA was subsequently cleaned, released, and eluted. The eluted DNA was used

(11) miR-381 modulates human bone mesenchymal stromal cells (BMSCs) osteogenesis via suppressing Wnt signaling pathway during atrophic nonunion developmentCell Death & DiseaseJuly 1, 2019Haitao Long, Yong Zhu, Zhangyuan Lin, Jun Wan, Liang Cheng, Min Zeng, Yifu Tang, Ruibo Zhaononimmune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc., NY, USA, P-2025-48). The fold enrichment (FE) was calculated as the ratio of the amplification efficiency of the ChIP

(12) lncRNA-NKILA/NF-κB feedback loop modulates laryngeal cancer cell proliferation, invasion, and radioresistanceCancer MedicineMay 1, 2018Tao Yang, Shisheng Li, Jiajia Liu, Danhui Yin, Xinming Yang, Qinglai TangIgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc., Farmingdale, NY, P‐2025‐48). The immunoprecipitated DNA was subsequently cleaned, released, and eluted. The eluted DNA was used

(13) The lncRNA XIST interacts with miR-140/miR-124/iASPP axis to promote pancreatic carcinoma growthOncotargetDecember 26, 2017Shuai Liang, Xuejun Gong, Gewen Zhang, Gengwen Huang, Yebin Lu, Yixiong Linon-immune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc., NY, USA, P-2025-48). The immunoprecipitated DNA was subsequently cleaned, released, and eluted. The eluted DNA was used

(14) MALAT1-miR-101-SOX9 feedback loop modulates the chemo-resistance of lung cancer cell to DDP via Wnt signaling pathwayOncotargetNovember 7, 2017Wei Chen, Wei Zhao, Li Zhang, Lixin Wang, Jipeng Wang, Zongren Wan, Yongqing Hong, Liang Yunon-immune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc., NY, USA, P-2025-48). The immunoprecipitated DNA was subsequently cleaned, released, and eluted. The eluted DNA was used

(15) Wild-type and mutant p53 differentially modulate miR-124/iASPP feedback following pohotodynamic therapy in human colon cancer cell lineCell Death & DiseaseOctober 1, 2017Kuijie Liu, Weidong Chen, Sanlin Lei, Li Xiong, Hua Zhao, Dong Liang, Zhendong Lei, Nanjiang Zhou, Hongliang Yao, Ying Liangwere used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc., Framingdale, NY, USA, P-2025-48). The immunoprecipitated DNA was subsequently cleaned, released and eluted. The eluted DNA was used

(16) Ferulic Acid Improves Functional Recovery after Acute Spinal Cord Injury in Rats by Inducing Hypoxia to Inhibit microRNA-590 and Elevate Vascular Endothelial Growth Factor ExpressionsFrontiers in Molecular NeuroscienceJune 8, 2017Zhenjie Li, Shengyun Wang, Wenfang Li, Hongbin Yuancontrol non-immune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc, P-2025-48). Binding efficiency (Bound/RPII%) was calculated by using a ratio of amplification efficiency of

(17) The miR-124-p63 feedback loop modulates colorectal cancer growthOncotargetApril 25, 2017Kuijie Liu, Hongliang Yao, Sanlin Lei, Li Xiong, Haizhi Qi, Ke Qian, Jiqiang Liu, Peng Wang, Hua Zhaonon-immune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc., NY, USA, P-2025-48). The immunoprecipitated DNA was subsequently cleaned, released, and eluted. The eluted DNA was used

(18) Nuclear factor-κB-dependent microRNA-130a upregulation promotes cervical cancer cell growth by targeting phosphatase and tensin homologArchives of biochemistry and biophysicsMay 15, 2016Feng Y, Zhou S, Li G, Hu C, Zou W, Zhang H, Sun L.

(19) TNF-α-induced NF-κB activation upregulates microRNA-150-3p and inhibits osteogenesis of mesenchymal stem cells by targeting β-cateninOpen BiologyMarch 4, 2016Nan Wang, Zubin Zhou, Tianyi Wu, Wei Liu, Peipei Yin, Chenhao Pan, Xiaowei Yucontrol non-immune IgG were used to demonstrate the efficacy of the kit reagents (Epigentek Group Inc, P-2025-48). Immunoprecipitated DNA is then cleaned, released and eluted. Eluted DNA can be used for downstream

(20) The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formationGenome biologyFebruary 22, 2016Guo H, Liu J, Ben Q, Qu Y, Li M, Wang Y, Chen W, Zhang J.mouse IgG, and GAPDH primers were used as controls to demonstrate the efficacy of the kit reagents (P-2025-48, Epigentek Group, Brooklyn, NY, USA). Purified chromatin was quantified by qRT-PCR using PowerUp™ SYBR® Green Master Mix

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ChromaFlash One-Step ChIP Kit

ChromaFlash One Step ChIP Kit from EpiGentek

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ChromaFlash One Step ChIP Kit from EpiGentek

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