EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit P-3001 from EpiGentek

Product Specs
ItemEpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
CompanyEpiGentek
Price Supplier Page View Company Product Page
Catalog NumberP-3001
Quantity48 Assays, 96 Assays
Sample TypeDNA
Research AreaDNA Methylation

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956

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Company Profile

Website: www.epigentek.com

(1) Ethanol Alters DNMT1/3a/3b Expression Profile, Promotes Persistent DNA Hypomethylation in Human Brain Endothelial Cells and Impairs Late Cortical AngiogenesisJ NeurochemFebruary 10, 2026Michele Siqueira, Matheus Barros, Paula Lacerda Almeida, Luiza Dos Santos Heringer, Henrique Rocha Mendonça, Flávia Carvalho Alcantara Gomes, Joice Stipurskyactivity using the EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay kit (Epigentek, Cat. P‐3001‐1), according to the manufacturer's recommendation. 2.9 Methylated DNA Immunoprecipitation (MeDIP) HBMEC

(2) Downregulation of ECRG4 by DNMT1 promotes EC growth via IRF3/IFN-γ/miR-29b/DNMT1/ECRG4 positive feedback loopiScienceJanuary 17, 2025Yang K, Chai S, Song H, Cao S, Gao F, Zhou C, Li L.activity with the EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Epigentek, No. P-3001-1) in accordance with the manufacturer’s instructions. Quantification and statistical analysis Data

(3) Milk derived extracellular vesicle uptake in human microglia regulates the DNA methylation machinery : Short title: milk-derived extracellular vesicles and the epigenetic machineryScientific reportsNovember 19, 2024Wijenayake S, Eisha S, Purohit MK, McGowan PO.activity was measured using the EpiQuick DNA Methyltransferase Activity/Inhibition Assay Kit (Epigentek; P-3001-96) as per manufacturer’s instructions. Total soluble protein lysates used for western immunoblotting

(4) Apigenin and its combination with Vorinostat induces apoptotic-mediated cell death in TNBC by modulating the epigenetic and apoptotic regulators and related miRNAsScientific ReportsApril 25, 2024Snehal Nimal, Navanath Kumbhar, Saruchi, Shriya Rathore, Nitin Naik, Sneha Paymal, Rajesh N. Gacchewas determined using the EpiQuik DNA methyltransferase activity assay kit (Epigentek, Base Catalog # P-3001) according to the manufacturer's protocol (https://www.epigentek.com/docs/P-3001.pdf) and earlier described

(5) Diabetic Retinopathy and Regulation of Mitochondrial Glutathione–Glutathione Peroxidase Axis in HyperhomocysteinemiaAntioxidants (Basel)February 20, 2024Pooja Malaviya, Renu A. Kowlurunuclear fraction (5 μg of protein) by an EpiQuik™ DNA Methyltransferase Activity assay kit (Cat. No. P-3001, EPIGENTEK) by quantifying methylated DNA using a 5-methylcytosine (5mC) antibody, as described previously

(6) Host DNA Demethylation Induced by DNMT1 Inhibition Up-Regulates Antiviral OASL Protein during Influenza a Virus InfectionVirusesJuly 28, 2023Zhao Z, Li J, Feng Y, Kang X, Li Y, Chen Y, Li W, Yang W, Zhao L, Huang S, et al.enzymatic activity was measured using an EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit (P-3001, Epigentek). The absorbance was measured at 450 nm using a microplate reader, and DNMT enzymatic activity

(7) DNA methylation changes underlie the long-term association between periodontitis and atherosclerotic cardiovascular diseaseFrontiers in Cardiovascular MedicineApril 21, 2023Mohamed Omar, Maria Alexiou, Umar R. Rekhi, Konrad Lehmann, Aneesh Bhardwaj, Cole Delyea, Shokrollah Elahi, Maria FebbraioTo determine DNMT activity, we used the EpiQuik DNA Methyltransferase Activity/Inhibition Assay Kit (P-3001-2, Epigentek) and followed the manufacturer's protocol. BMDM nuclear extracts were used in this assay.

(8) Enriched environment causes epigenetic changes in hippocampus and improves long-term cognitive function in sepsisScientific reportsJuly 7, 2022Córneo E, Michels M, Abatti M, Vieira A, Gonçalves RC, Gabriel FF, Borges H, Goulart A, da Silva Matos N, Dominguini D, et al.detection) according to the manufacturer's instructions (Epigentek cod. HAT P-4003-96; HDAC P-4002-96; DNMT P-3001-2). A standard curve was performed with serial dilutions of subtracts from each kit and positive and

(9) Luteolin Causes 5′CpG Demethylation of the Promoters of TSGs and Modulates the Aberrant Histone Modifications, Restoring the Expression of TSGs in Human Cancer CellsInternational Journal of Molecular SciencesApril 6, 2022Sreepoorna Pramodh, Ritu Raina, Arif Hussain, Sali Abubaker Bagabir, Shafiul Haque, Syed Tasleem Raza, Mohammad Rehan Ajmal, Shalini Behl, Deepika Bhagavatula(Catalogue number OP-0002; Epigentek, NY, USA). The EpiQuik DNMT activity assay kit (Catalogue number P-3001; Epigentek, NY, USA) was used to check the effect of the luteolin on DNMT activity. In the sample wells

(10) Novel Targeting of DNA Methyltransferase Activity Inhibits Ewing Sarcoma Cell Proliferation and Enhances Tumor Cell Sensitivity to DNA Damaging Drugs by Activating the DNA Damage ResponseFrontiers in endocrinologyJanuary 1, 2022Cristalli C, Manara MC, Valente S, Pellegrini E, Bavelloni A, De Feo A, Blalock W, Di Bello E, Piñeyro D, Merkel A, et al.Context DNMT activity was quantified with an EpiQuick DNA Methyltransferase Activity/Inhibition Assay Kit (P-3001-1, Epigentek Inc., Farmingdale, NY, U.S.). Cells were treated with MC3343 or 5azadC (3-10 µM) for 48

(11) The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturationPloS oneJanuary 1, 2021Sternbach S, West N, Singhal NK, Clements R, Basu S, Tripathi A, Dutta R, Freeman EJ, McDonough J.with an EpiQuick DNA Methyltransferase Activity/Inhibition Assay Kit (Epigentek Inc., Farmingdale, NY, P-3001-1). Briefly, MO3.13 cells were treated with either a scrambled or targeting siRNA oligonucleotide for

(12) Homocysteine Disrupts Balance between MMP-9 and Its Tissue Inhibitor in Diabetic Retinopathy: The Role of DNA MethylationInternational journal of molecular sciencesMarch 5, 2020Mohammad G, Kowluru RA.Activity/Inhibition and Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kits, respectively (Cat. Nos. P-3001 and P-3087; EPIGENTEK, Farmingdale, NY, USA), as described previously [19]. 4.7. Immunofluorescence The

(13) The DNMT1/miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like CellsStem Cells InternationalOctober 31, 2019Xiao Liang, Chang Xu, Wanchun Wang, Xiang Liactivity is nonradioactively measured by using a DNA Methyltransferase Activity/Inhibition Assay Kit (cat. P-3001; EpiGentek Group Inc., Farmingdale, NY, USA) according to the protocol. DNMT activity was calculated

(14) Reduced PPARγ2 expression in adipose tissue of male rat offspring from obese dams is associated with epigenetic modificationsFASEB journal : official publication of the Federation of American Societies for Experimental BiologyMay 1, 2018Lecoutre S, Pourpe C, Butruille L, Marousez L, Laborie C, Guinez C, Lesage J, Vieau D, Eeckhoute J, Gabory A, et al.activity 241 DNMT, TET, HAT and HDAC activities were studied using the EpiQuick DNMT 242 Assay kit (Cat # P-3001-2; Epigentek, Mundolsheim, France), TET Hydroxylase Activity 243 Quantification kit (Cat # ab156912;

(15) Characterization of Cytosine Methylation and the DNA Methyltransferases of Toxoplasma gondiiInternational Journal of Biological SciencesMarch 11, 2017Haixia Wei, Shichen Jiang, Longfei Chen, Cheng He, Shuizhen Wu, Hongjuan Pengassay. DNMT activity was measured using the EpiQuik DNA Methyltransferase Activity Kit (EpigenTek Cat. #P-3001, Colorimetric). Assays were conducted in triplicate on three independent preparations of detection samples

(16) Peperomin E reactivates silenced tumor suppressor genes in lung cancer cells by inhibition of DNA methyltransferaseCancer scienceOctober 1, 2016Wang XZ, Cheng Y, Wang KL, Liu R, Yang XL, Wen HM, Chai C, Liang JY, Wu H.USA). The DNMT activity was determined using an Epiquik DNMT activity/inhibition assay kit (Cat. No. P‐3001; Epigentek) following the manufacturer's instructions. Briefly, 10 μg A549 nuclear extract was added

(17) Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicalsEpigenomicsAugust 1, 2016Li Y, Buckhaults P, Cui X, Tollefsbol TO.

(18) Ensemble-Based Virtual Screening and Experimental Validation of Inhibitors Targeting a Novel Site of Human DNMT1Chemical biology & drug designJuly 1, 2016Manali Joshi, Shriram N Rajpathak, Santosh C Narwade, Deepti Deobagkar

(19) Reduction in promotor methylation utilizing EGCG (epigallocatechin-3-gallate) restores RXRα expression in human colon cancer cellsOncotargetJune 7, 2016Morris J, Moseley VR, Cabang AB, Coleman K, Wei W, Garrett-Mayer E, Wargovich MJ.directions. DNMT inhibition was analyzed using EpiQuik DNA Methyltransferase Activity/Inhibition Assay Kit (P-3001-2, Epigentek) following the manufacturer's directions. Statistical analysis P-values were calculated

(20) The green tea polyphenol EGCG alleviates maternal diabetes–induced neural tube defects by inhibiting DNA hypermethylationAmerican Journal of Obstetrics and GynecologyMarch 7, 2016Jianxiang Zhong, Cheng Xu, E. Albert Reece, Peixin Yang

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EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit from EpiGentek

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EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit from EpiGentek

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