Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) P-4034 from EpiGentek

Product Specs
ItemEpigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric)
CompanyEpiGentek
Price Supplier Page View Company Product Page
Catalog NumberP-4034
Quantity48 Assays, 96 Assays
Sample TypeNuclear Extracts, Purified Enzyme
Research AreaHistone Acetylation

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
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Website: www.epigentek.com

(1) Epigenetically driven impairment of BDNF-ARC signaling contributes to circadian and cognitive disarray in a mouse model of postoperative deliriumAlzheimers DementJune 15, 2026Hari Prasad Osuru, Navya Atluri, Tam Le, Jinny Park, Michal Jedrusiak, Elzbieta Dulko, Meghana Illendula, Nadia LunardiActivity Assay Kit (Cat#P‐4003), and HDAC activity was measured using the HDAC Activity Assay Kit (Cat#P‐4034), both from Epigentek (New York, USA). Assays were performed according to the manufacturer's protocols

(2) Metabolite mimicry identifies butyrate analogs with select protective functions in the intestinal mucosaProceedings of the National Academy of SciencesApril 14, 2026Alfredo Ornelas, Jacob A. Countess, Ji Yeon Kim, Rachel H. Cohen, Brittany D. Gomez, Rebecca L. Roer, Faiz Minhajuddin, Kiranmayee Yenugudhati Vijaya Yenugudhati Vijaya Sai, Liheng Zhou, Julia L. M. Dunn, et al.individual plots. HDAC inhibition assay- HDAC activity/inhibition were measured using Epigentek kits (P-4034-96) following their protocol without modifications. Briefly, Caco-2 cells were grown to confluency in

(3) Epigenetic Suppression of RASAL1 by HDAC3 and Cofactor YY1 Promotes Fibroblast–Myofibroblast Transition and Renal FibrosisResearch (Wash D C)January 29, 2026Fang Chen, Lijun Zhang, Weiying Liu, Bingbing Zhang, Shuren Wang, Zhengdong Zhou, Wei Wang, Jiansong Shen, Yijun Deng, Wangsen Caoof kidney tissues were assessed using an HDAC activity/inhibition direct assay kit (colorimetric) (P-4034, Epigentek, USA). Following the manufacturer’s instructions, the acetylated substrates were incubated

(4) Effects of Methylglyoxal on Intestinal Cells: Insights on Epigenetic Regulatory EnzymesIUBMB LifeDecember 8, 2025Camilla Morresi, Giulia Feliziani, Luisa Bellachioma, Christian Giommi, Rosita Gabbianelli, Laura Bordoni, Gianna Ferretti, Tiziana Bacchetti, Elisabetta DamianiKit (P‐3086, Epigentek, Farmingdale, NY, USA), Epigenase HDAC Activity/Inhibition Direct Assay Kit (P‐4034, Epigentek), or EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (P‐3139, Epigentek), respectively [41,

(5) Epigenetic Reprogramming Via Sodium Butyrate Induces Corneal Myofibroblast Dedifferentiation In Vitro and Inhibits Fibrosis In VivoInvestigative Ophthalmology & Visual ScienceNovember 24, 2025Swati Sood, Nishant R. Sinha, Anil Tiwari, Eva Ruterschmidt, Ratnakar Tripathi, Suneel Gupta, Rajiv R. Mohanthe nuclear extract with Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric; Cat no. P-4034; EpigenTek, Farmingdale, NY, USA) following manufacturer's instructions. This kit allows measurement

(6) Stress during puberty and adulthood pregnancy impact histone acetylation regulators in the hypothalamusNeuroscienceMay 14, 2025Laiklyn A.M. Luther, Samantha L. Higley, Kathleen E. MorrisonHDAC activity was measured using the Epigenase HDAC Activity/Inhibition Direct Assay Kit (Epigentek, P-4034). Assays were conducted without deviation from manufacturer’s instructions. A range of acceptable protein

(7) Viscoelastic extracellular matrix enhances epigenetic remodeling and cellular plasticityNat CommunApril 30, 2025Yifan Wu, Yang Song, Jennifer Soto, Tyler Hoffman, Xiao Lin, Aaron Zhang, Siyu Chen, Ramzi N. Massad, Xiao Han, Dongping Qi, et al.the HAT activity/inhibition assay (EpiGentek, P-4003-048), HDAC activity/inhibition assay (EpiGentek, P-4034-096), HMT (H3K4 specific) activity/inhibition assay (EpiGentek, P-3002-1), and HDM (H3K4 specific) activity/inhibition

(8) Gestational Inulin Supplementation in Low-/High-Fat Sow Diets: Effects on Growth Performance, Lipid Metabolism, and Meat Quality of Offspring PigsFoodsApril 10, 2025Pan Zhou, Yachao Wu, Jianbo Shen, Tao Duan, Long Che, Yong Zhang, Yang Zhao, Honglin Yanmeasured using the Epigenase™ HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) (Base Catalog #P-4034; Epigentek, Farmingdale, NY, USA) according to the manufacturer’s protocol. The principle of the assay

(9) Reversal of epithelial to mesenchymal transition in triple negative breast cancer through epigenetic modulations by dietary flavonoid Galangin and its combination with SAHACell Commun SignalApril 2, 2025Snehal Nimal, Navanath Kumbhar, Manasi S. Pote, Rahul Bankar, Mahemud Shaikh, Rajesh Gacche(Cat No. OP-0002-1). HDAC activity was measured using the Epigentek HDAC Assay Kit (Base Catalog # P-4034) per manufacturer instructions. The activity was calculated using the formula: HDAC Activity (OD/min/mg)

(10) Unveiling the mechanism of broad‐spectrum blast resistance in rice: The collaborative role of transcription factor OsGRAS30 and histone deacetylase OsHDAC1Plant Biotechnology JournalJune 1, 2024Jiaqi Hou, Huangzhuo Xiao, Peng Yao, Xiaoci Ma, Qipeng Shi, Jin Yang, Haoli Hou, Lijia LiHDAC activity assays using the Epigenase HDAC activity/inhibition direct assay kit (Epigentek, Cat. # P‐4034, Farmingdale, NY, USA) in accordance with the manufacturer's instructions. After the reactions were

(11) Apigenin and its combination with Vorinostat induces apoptotic-mediated cell death in TNBC by modulating the epigenetic and apoptotic regulators and related miRNAsScientific ReportsApril 25, 2024Snehal Nimal, Navanath Kumbhar, Saruchi, Shriya Rathore, Nitin Naik, Sneha Paymal, Rajesh N. GaccheHDAC activity measurement was performed using a fluorometric HDAC assay kit (Epigentek, Base Catalog # P-4034) according to the manufacturer’s instructions (https://www.epigentek.com/docs/P-4034.pdf). Briefly,

(12) Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver diseaseeBioMedicineMarch 1, 2024Carlos Jose Pirola, Adrian Salatino, Tomas Fernández Gianotti, Gustavo Osvaldo Castaño, Martin Garaycoechea, Silvia SookoianEpiQuick HAT Activity/Inhibition Assay Kit (P-4003) and the EpiQuick HDAC Activity/Inhibition Assay Kit (P-4034) from Epigentek, Inc. (Farmingdale, NY 11735, USA) in accordance with the manufacturer’s protocol. The

(13) HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progressionRedox BiologyDecember 1, 2023Lijun Zhang, Fang Chen, Jian Dong, Rong Wang, Guangyu Bi, Daoliang Xu, Yingwei Zhang, Yijun Deng, Wenjun Lin, Zhongzhou Yang, et al.activities of renal tissues were analyzed with an HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) (P-4034, Epigentek, USA) according to the manufactory's protocol. In brief, the acetylated substrates were incubated

(14) HSV-2 triggers upregulation of MALAT1 in CD4+ T cells and promotes HIV latency reversalJournal of Clinical InvestigationJune 1, 2023Carl A. Pierce, Lip Nam Loh, Holly R. Steach, Natalia Cheshenko, Paula Preston-Hurlburt, Fengrui Zhang, Stephanie Stransky, Leah Kravets, Simone Sidoli, William M Philbrick, et al.nuclear extract was used to determine HDAC activity (Epigenase HDAC Activity/Inhibition Direct Assay Kit, P-4034-96, EpigenTek). Absorbance was measured using a SpectraMax M5e microplate reader and SoftMax Pro 7.1 GxP software

(15) HSV-2 triggers upregulation of MALAT1 in CD4+ T cells and promotes HIV latency reversalJ Clin InvestJune 1, 2023Carl A. Pierce, Lip Nam Loh, Holly R. Steach, Natalia Cheshenko, Paula Preston-Hurlburt, Fengrui Zhang, Stephanie Stransky, Leah Kravets, Simone Sidoli, William Philbrick, et al.nuclear extract was used to determine HDAC activity (Epigenase HDAC Activity/Inhibition Direct Assay Kit, P-4034-96, EpigenTek). Absorbance was measured using a SpectraMax M5e microplate reader and SoftMax Pro 7.1 GxP software

(16) Differential histone deacetylase inhibitor‐induced perturbations of the global proteome landscape in the setting of high‐grade serous ovarian cancerPROTEOMICSFebruary 1, 2023Jolene M. Duda, Carly A. I. Twigg, Stefani N. ThomasSelleck Chemicals. Nuclear Extraction kit (40010) was purchased from Active Motif. HDAC Activity Assay kit (P-4034–96) was purchased from Epigentek. MTT assay kit was purchased from Abcam (ab211091). TMTpro 16plex Label

(17) Histone deacetylase-2 controls IL-1β production through the regulation of NLRP3 expression and activation in tuberculosis infectioniScienceAugust 19, 2022Moreira JD, Iakhiaev A, Vankayalapati R, Jung BG, Samten B.assays,Critical commercial assays ,, Epigenase HDAC Activity/Inhibition Direct Assay Kit,Epigentek,Cat: P-4034 Human IL-1β ELISA BASIC kit (HRP),Mabtech,Cat: 3416-1H-20 ELISA MAX™ Standard Set Human IL-6,Biolegend,"Cat:

(18) Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibitionTheranosticsJanuary 1, 2022Huang G, Cheng Z, Hildebrand A, Wang C, Cimini M, Roy R, Lucchese AM, Benedict C, Mallaredy V, Magadum A, et al.protein was used to measure HDAC activity according to the manufacturer's instructions (Epigentek, P-4034-48). RNA extraction, reverse transcription, and RT-PCR RNA was isolated from cells using miRNeasy Mini kit

(19) Environmental enrichment improves lifelong persistent behavioral and epigenetic changes induced by early-life stressJournal of Psychiatric ResearchJune 1, 2021Laura A. Borba, Lia D.R. Broseghini, Luana M. Manosso, Airam B. de Moura, Maria Eduarda M. Botelho, Camila O. Arent, João Paulo Behenck, Amanda Hilsendeger, Letícia H. Kammer, Samira S. Valvassori, et al.manufacturer’s instructions. Briefly, nuclear extract samples were mixed with HDAC (EPIGENTEK; Base catalog #P-4034) or DNMT (EPIGENTEK; Base catalog #P-3009) assay buffer, more assay substrate of the same enzymes, in

(20) Acute high folic acid treatment in SH-SY5Y cells with and without MTHFR function leads to gene expression changes in epigenetic modifying enzymes, changes in epigenetic marks, and changes in dendritic spine densitiesPLOS ONEJanuary 7, 2021Daniel F. Clark, Rachael Schmelz, Nicole Rogers, Nuri E. Smith, Kimberly R. Shorter(H3K4) (Cat. No. P-3002-1), HATs (Cat. No. P-4003-48), TETs (Cat. No. P-3086-48), and HDACs (Cat. No. P-4034-48) were used. Nuclear extracts were loaded in equal amounts into the plates in duplicate. Antibodies

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Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric)

Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) from EpiGentek

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Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) from EpiGentek

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