rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit AS-FS-004 from Arraystar, Inc

Product Specs
ItemrtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit
CompanyArraystar, Inc
Price $205.00Supplier Page View Company Product Page
Catalog NumberAS-FS-004
TypetRNA
ApplicationscDNA Synthesis
Quantity12RXNs

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Arraystar, Inc

9430 Key West Avenue

Suite 128

Rockville, Maryland 20850

United States

Phone: 888-416-6343
240-314-0388
Fax: 240-238-9860

Company Profile

Website: http://www.arraystar.com

(1) Targeting tRNA-dependent tyrosine usage unveils a metabolic vulnerability in hepatocellular carcinomaNat CommunMarch 6, 2026Hongli Zhang, Zixuan Wang, Yiqi Zhao, Xiya Deng, Jian Zhang, Fei Shang, Hongjie Zhang, Yan Liu, Hongbo Wang, Yating Yang, et al.A260/A280 ratio. Total tRNA quantification was performed using a tRNA quantification kit (Arraystar, AS-FS-004). Briefly, purified RNA was treated with demethylase AlkB to remove methylation. Following RNA purification

(2) Context-specific Angiogenin-mediated tRNA fragments (tDRs) biogenesis shapes the mitochondrial stress responseRedox BiologyMarch 1, 2026Shadi Al-Mesitef, Daisuke Ando, Tomoya Saigasaki, Yuki Sakaguchi, Shunya Akagi, Abdulrahman Mousa, Sherif Rashad, Kuniyasu NiizumaAlkB demethylase treatment (rtStar tRNA-optimized First-Strand cDNA Synthesis Kit, ArrayStar, Cat# AS–FS–004) at RT for 2 h to mitigate tRNA methylation. Purification of RNA employed the Zymo Directzol RNA micro

(3) PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcomaCellMay 15, 2025Bhargab Kalita, Gerard Martinez-Cebrian, Justina McEvoy, Melody Allensworth, Michelle Knight, Alessandro Magli, Rita C.R. Perlingeiro, Michael A. Dyer, Elizabeth Stewart, Brian David Dynlachtfirst-strand cDNA synthesis using rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit (Arraystar Inc., Cat# AS-FS-004) according to manufacturer’s protocol. Individual tRNA expression was normalized to U6 and was scored

(4) Decoding Codon Bias: The Role of tRNA Modifications in Tissue-Specific TranslationInt J Mol SciJanuary 15, 2025Daisuke Ando, Sherif Rashad, Thomas J. Begley, Hidenori Endo, Masashi Aoki, Peter C. Dedon, Kuniyasu Niizumademethylase (rtStar tRNA-optimized First-Strand cDNA Synthesis Kit, ArrayStar, Rockville, MD, USA, #AS-FS-004) at ambient temperature for 2 h to reduce tRNA methylation. RNA was purified using a Zymo Directzol

(5) Non-coding RNAs secreted by renal cancer include piR_004153 that promotes migration of mesenchymal stromal cellsCell Commun SignalJanuary 3, 2025Joanna Bogusławska, Małgorzata Grzanka, Piotr Popławski, Weronika Zarychta-Wiśniewska, Anna Burdzinska, Karolina Hanusek, Helena Kossowska, Roksana Iwanicka-Nowicka, Alex Białas, Beata Rybicka, et al.was performed by using the rtStar™ tRNA - optimized First-Strand cDNA Synthesis Kit (Arraystar, Cat#: AS-FS-004, Rockville, MD, USA). The manufacturer’s protocol of RNA demethylation was modified by reducing the

(6) Nucleotidyltransferase toxin MenT extends aminoacyl acceptor ends of serine tRNAs to control Mycobacterium tuberculosis growthNature CommunicationsNovember 6, 2024Xibing Xu, Roland Barriot, Bertille Voisin, Tom J. Arrowsmith, Ben Usher, Claude Gutierrez, Xue Han, Carine Pagès, Peter Redder, Tim R. Blower, et al.modifications in the tRNAs, the total RNA samples were pretreated using the demethylase kit (Arraystar, cat#: AS-FS-004, Rockville, MD, USA), followed by trizol isolation. Notably, during the demethylation reaction (pH 7.5–8)58,59,

(7) Valine aminoacyl-tRNA synthetase promotes therapy resistance in melanomaNature cell biologyJuly 1, 2024El-Hachem N, Leclercq M, Susaeta Ruiz M, Vanleyssem R, Shostak K, Körner PR, Capron C, Martin-Morales L, Roncarati P, Lavergne A, et al.reprecipitation and first-strand cDNA synthesis (rtStar tRNA Pretreatment and First-Strand cDNA Synthesis kit, AS-FS-004, Arraystar). These steps were performed according to the manufacturer’s recommendations. Quantitative evaluation

(8) The m5 C methyltransferase NSUN2 promotes codon-dependent oncogenic translation by stabilising tRNA in anaplastic thyroid cancerClinical and Translational MedicineNovember 20, 2023Peng Li, Wenlong Wang, Ruixin Zhou, Ying Ding, Xinying Lisamples were reprecipitated and resuspended in RNase‐free water. Then rtStar™ tRNA Pretreatment Kit (#AS‐FS‐004; Arraystar) was used to demethylate. Two micrograms of RNA harvested was used to perform qRT‐PCR using

(9) MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin bindingNat CommunAugust 17, 2023Xibing Xu, Ben Usher, Claude Gutierrez, Roland Barriot, Tom J. Arrowsmith, Xue Han, Peter Redder, Olivier Neyrolles, Tim R. Blower, Pierre Genevauxmodification in the tRNAs, the total RNA samples were pretreated using the demethylase kit (Arraystar, cat#: AS-FS-004, Rockville, MD, USA), followed by trizol isolation. The 3p-v4 oligo was 5′ adenylated using 5′ DNA Adenylation Kit

(10) N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicingNucleic Acids ResearchAugust 11, 2023Can Wang, Nathalie Ulryck, Lydia Herzel, Nicolas Pythoud, Nicole Kleiber, Vincent Guérineau, Vincent Jactel, Chloé Moritz, Markus T Bohnsack, Christine Carapito, et al.reverse transcription were performed with rtStar tRNA-optimized First-Strand Synthesis Kit (Arraystar, #AS-FS-004). qPCR for tRNAs was performed using the nrStar Human tRNA PCR Array (Arraystar, #AS-NR-001–1) and Arraystar

(11) GANT-61 induces cell cycle resting and autophagy by down-regulating RNAP III signal pathway and tRNA-Gly-CCC synthesis to combate chondrosarcomaCell Death & DiseaseJuly 24, 2023Yifeng Sun, Qiongxuan Fang, Wei Liu, Yi Liu, Chunming Zhangprimer sets perform superbly with the rtStar™ tRNA Pretreatment & First-Strand cDNA Synthesis Kit (CAT# AS-FS-004) and Arraystar SYBR Green qPCR Master Mix. Transmission electron microscopy Fresh tumor tissues were

(12) A specific tRNA half, 5’tiRNA-His-GTG, responds to hypoxia via the HIF1α/ANG axis and promotes colorectal cancer progression by regulating LATS2Journal of Experimental & Clinical Cancer ResearchDecember 1, 2021En-Wei Tao, Hao-Lian Wang, Wing Yin Cheng, Qian-Qian Liu, Ying-Xuan Chen, Qin-Yan GaotRNA-His-GTG was reverse transcribed to cDNA using rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit (#: AS-FS-004, Arraystar). Then, qPCR was performed with SYBR Premix Ex Taq (Takara). The expression levels of tsRNAs

(13) Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cellsPLoS geneticsNovember 1, 2021Chen M, Long Q, Borrie MS, Sun H, Zhang C, Yang H, Shi D, Gartenberg MR, Deng W.reverse transcription were performed with rtStar tRNA-optimized First-Strand Synthesis Kit (Arraystar AS-FS-004). qPCR for tRNAs was performed using the nrStar Human tRNA PCR Array (Arraystar AS-NR-001-1) and Arraystar

(14) Mistranslation Drives Alterations in Protein Levels and the Effects of a Synonymous Variant at the Fibroblast Growth Factor 21 LocusAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2021Bayoumi A, Elsayed A, Han S, Petta S, Adams LA, Aller R, Khan A, García-Monzón C, Arias-Loste MT, Miele L, et al.rtStar tRNA‐optimized First‐Strand cDNA Synthesis Kit (Arraystar, Inc. Rockville, MD 20850, USA, Cat#: AS‐FS‐004) according to the manufacturer's instructions. This kit used reverse transcriptase at high temperature

(15) Neurons Release Serine to Support mRNA Translation in Pancreatic CancerCellNovember 25, 2020Banh RS, Biancur DE, Yamamoto K, Sohn ASW, Walters B, Kuljanin M, Gikandi A, Wang H, Mancias JD, Schneider RJ, et al.,R&D Systems,DY256 NAD/NADH-Glo™ Assay,Promega,G9071 rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit,Array Star,AS-FS-004 ,, Deposited Data,, Ribosomal Profiling,This study,GSE139910 tRNA-seq,This study,GSE139910 Experimental Models

(16) tRNA-derived fragments (tRFs) regulate post-transcriptional gene expression via AGO-dependent mechanism in IL-1β stimulated chondrocytesOsteoarthritis and CartilageAugust 1, 2020J. A. Green, M. Y. Ansari, H.C. Ball, T. M. HaqqiFirst-Strand cDNA Synthesis: The rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit (ArrayStar Cat # AS-FS-004) was used to create cDNA libraries from tRNAs. The tRNA modifications such as 3’-aminoacyl and 3’-cP

(17) Gene-Specific Control of tRNA Expression by RNA Polymerase IIMolecular cellMay 21, 2020Gerber A, Ito K, Chu CS, Roeder RG.using the reagents included in the rtStar tRNA-optimized first-strand cDNA synthesis kit (Arraystar #AS-FS-004) following manufacturer’s instructions except first-strand cDNA synthesis was omitted and, instead,

(18) Schlafen 11 Restricts Flavivirus ReplicationJournal of virologyAugust 1, 2019Valdez F, Salvador J, Palermo PM, Mohl JE, Hanley KA, Watts D, Llano M.by ﬁrst-strand cDNA synthesis (rtStar tRNA-optimized ﬁrst-strand cDNA synthesis kit, catalog number AS-FS-004; Arraystar). Real-time PCR was then performed using a proprietary human tRNA repertoire PCR array that

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rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit from Arraystar, Inc

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Arraystar, Inc

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rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit from Arraystar, Inc

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