Human GM-CSF ELISA Development Kit CKERS-CSF2-023H from Creative Diagnostics

Product Specs
ItemHuman GM-CSF ELISA Development Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberCKERS-CSF2-023H
Quantity5 plates;15 plates
ApplicationsELISA
Temperature##Capture Antibody: ## 360 µg of antigen-affinity purified goat anti-GM-CSF. Centrifuge vial prior to opening. Reconstitute in 1.0 mL PBS for a concentration of 360 µg/mL. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
##Detection Antibody: ##90 µg of biotinylated antigen-affinity purified goat anti-GM-CSF. Centrifuge vial prior to opening. Reconstitute in 1.0 mL sterile 1.0% BSA in PBS for a concentration of 90 µg/mL. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
##Human GM-CSF Standard: ##55 µg of recombinant GM-CSF. Centrifuge vial prior to opening. Reconstitute in 0.5 mL sterile water for a concentration of 100 ng/mL. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer.
##UltraAvidin-HRP Conjugate: ##40 µL vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
##TMB Liquid Substrate: ##Aspirate and wash plate 4 times. Add 100 µL of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitorcolor development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570 nm. The reaction may be stopped after 15-20 minutes by adding 100 µL of 2 M sulfuric acid to each well
ComponentsPBS: Dilute 10xPBS to 1xPBS, pH 7.2 in sterile water.
Wash Buffer: 0.05% Tween-20 in PBS
Block Buffer: 1.0% BSA in PBS
Diluent: 1.0% BSA in PBS
ELISA microplates (Thermo Fisher Cat. # 456529);
BSA (Sigma Cat. # A-7030);
Stop Solution 2 M Sulfuric Acid (Sigma Cat. # 339741);
Dulbecco's PBS [10x] (Gibco BRL Cat. # 14200-075)
Sample TypeTBD
ReactivityHuman
Detection TargetCSF2

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Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

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Website: https://www.creative-diagnostics.com/

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Human GM-CSF ELISA Development Kit from Creative Diagnostics

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Human GM-CSF ELISA Development Kit from Creative Diagnostics

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