Rat IL-1β(Interleukin 1 Beta) ELISA Kit CKE-IL-1B-042R from Creative Diagnostics

Product Specs
ItemRat IL-1β(Interleukin 1 Beta) ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberCKE-IL-1B-042R
Quantity96T
ApplicationsELISA
Detection Range31.25-2000pg/ml
ReactivityRat
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Detection TargetIL1B
TemperatureCapture Antibody: 200µg of antigen-affinity purified goat anti-rat IL-1β+ 2.5mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 mL sterile water for a concentration of 200µg/mL.
Detection Antibody: 50µg of biotinylated antigen-affinity purified goat Anti-rat IL-1β+2.5mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100µg/mL.
Rat IL-1β Standard: 1µg of recombinant rat IL-1β+ 2.2mg BSA+11.0mg D-mannitol. Centrifuge vial prior to opening. Reconstitute in 1 mL sterile water for a concentration of 1µg/mL.
NOTE: The reconstituted components are stable for 2 weeks when stored at 2-8°C. Components that have been reconstituted and aliquoted can be stored at -20°C for up to 6 months.
Avidin-HRP Conjugate: 60µL vial. Upon receipt, avidin-HRP conjugate should be aliquoted into ten 6µL vials and stored at <-20°C. Aliquots stored frozen at <-20°C are stable for up to 2 years from date of receipt.
Avoid more than one freeze-thaw cycle
ComponentsAll solutions should be at ambient temperature prior to use.
PBS: Dilute 10xPBS to 1xPBS, pH 7.20 in sterile water.
Wash Buffer: 0.05% Tween-20 in PBS
Block Buffer: 1% BSA in PBS *
Diluent: 0.05% Tween-20, 0.1% BSA in PBS *
* Sterile filter and store at 4oC for up to 1 week.
ELISA microplates (Nunc Maxisorp Prod. #442404);
Tween-20 (Sigma Cat. # P-7949);
BSA (Sigma Cat # A-7030);
TMB HRPO Microwell Substrate Standard Kinetic
Dulbecco's PBS [10x] (Gibco BRL Cat.#14200-075)

Add to Compare List

Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

Be the First to Write a Review!

Rat IL-1β(Interleukin 1 Beta) ELISA Kit from Creative Diagnostics

Description

Creative Diagnostics

Reviews

Rat IL-1β(Interleukin 1 Beta) ELISA Kit from Creative Diagnostics

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map