Description
Background/Introduction: Glycolate oxidase is a member of the superfamily of the a-hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals. It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. The assay is initiated with the enzymatic oxidization of the Glycolic acid by Glycolate oxidase. The enzyme catalysed reaction product Glyoxylic acid react with Phenylhydrazine, glyoxylate phenylhydrazone can be measured at a colorimetric readout at 324 nm