DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) 14209S from Cell Signaling Technology

Product Specs
ItemDNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag)
CompanyCell Signaling Technology
Price $131.00 Inquire
Catalog Number14209S
Quantity1 Kit
Research AreaChromatin Regulation / Nuclear Function
Nucleic Acid TypeChIP DNA
ApplicationsChIP, C&R, C&T
FormatSpin column

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Prolonged KRAS-MAPK Inhibition Induces Interferon Signaling that Promotes Cell State Transition and Confers Therapeutic VulnerabilitiesCancer ResApril 20, 2026Ashenafi Bulle, Yali Chen, Huaping Li, Timothy Hung-Po Hung-Po Chen, Iftikhar Ali Khawar, Lin Li, Yu Wang, Peng Liu, Vikas Kumar Somani, Richard Kurupi, et al.fragments, which were then purified using the DNA Spin Column Kit (Cell Signaling Technology, Cat# 14209). Sequencing libraries were prepared using the DNA Library Prep Kit for Illumina Systems (Cell Signaling Technology

(2) The transcription elongation factors Spt4 and Spt6 promote dermal adipocyte differentiationAdipocyteMarch 27, 2026Julian Gomez, Samiksha Mahapatra, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Neha Quadir, George L. SenDNA was purified using DNA purification buffers and spin columns kit (Cell Signalling Technology, #14209). Input samples were mixed with Extraction Buffer (Proteinase K+RNAse A) and incubated at 55°C for 1

(3) Identification of cis-regulatory elements provides insights into tissue-specific gene regulation in the sheep genomeNat CommunMarch 16, 2026Shangqian Xie, Kimberly M. Davenport, Mazdak Salavati, Alex Caulton, Emily L. Clark, Alan L. Archibald, Shannon M. Clarke, Rudiger Brauning, Alisha T. Massa, Michelle R. Mousel, et al.Buffers and Spin Columns Kit following manufacturer’s instructions (Cell Signaling Technologies, cat. #14209, Danvers, MA, USA). Purified DNA was quantified using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific,

(4) An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progressioniScienceFebruary 20, 2026Amber J. Kiliti, Ghada M. Sharif, Megan E. McNamara, Raneen Rahhal, Susan Prewitt, Marcel O. Schmidt, Ci Wu, Junfeng Ma, Eric Glasgow, Anton Wellstein, et al.hours. DNA was purified using DNA Purification Buffers and Spin Columns (Cell Signaling Technology, 14209) and libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645).

(5) Epigenetic and Transcriptional Programs Define Osteosarcoma Subtypes and Establish Targetable VulnerabilitiesCancer DiscoveryFebruary 6, 2026Eunice López-Fuentes, Andrew S. Clugston, Alex G. Lee, Leanne C. Sayles, Natalie Sorensen, Maria V. Pons Ventura, Stanley G. Leung, Truc Dinh, Marcus R. Breese, E. Alejandro Sweet-Corderosamples was purified using the DNA Purification Buffers and Spin Columns kit (Cell Signaling Technology, #14209). DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs

(6) Multi-Omics Analysis of Human Blood Cells Reveals Unique Features of Age-Associated Type 2 CD8 Memory T CellsAging CellFebruary 1, 2026Hiroyuki Matsui, Marlene Cervantes, Mir M. Khalid, Alan Tomusiak, Varun Dwaraka, Jorge Landgrave-Gomez, Prasanna Vadhana Ashok Kumaar, Qingwen Chen, Jessica Lasky-Su, Jake Stone, et al.with the addition of proteinase K and RNAse A. Fragmented DNA was purified using Spin Columns (CST#14209) per manufacturer's protocol. 5.1.10 RNA‐seq Data Processing and Analysis Quality control and trimming

(7) Regulatory role for tumor suppressor REST on estrogen receptor (ESR1) expression and leiomyoma pathophysiologyJournal of Biological ChemistryJanuary 1, 2026Skylar G. Bird, Sumedha Gunewardena, Ashley Cloud, Sornakala Ganeshkumar, Vargheese M. Chennathukuzhiconfluency, both the cells and biopulverized tissue underwent standard ChIP kit protocol (Cell Signaling, #14209S, Cell Signaling Technology RRID:SCR_002071). Antibodies used for ChIP included rabbit IgG and H3XP as

(8) Menin-driven mTOR signaling sustains taxane resistance in CRPC and reveals a targetable vulnerability for combination therapyCell Communication and SignalingDecember 18, 2025Ipek Bulut, Buse Cevatemre, Neslihan Yuksel-Catal, Hamzah Syed, Tamer Onder, Ceyda AcilanDNA was then purified using spin columns, following the protocol of the CST DNA Purification Kit (#14209). Sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

(9) A cholesterol-responsive hepatic tRNA-derived small RNA regulates cholesterol homeostasis and atherosclerosis developmentNat CommunDecember 15, 2025Xiuchun Li, Rebecca Hernandez, Xudong Zhang, Sijie Tang, Xiaohong Yuan, Jing Wu, Kathy Pham, Hukam C. Rawal, Erica C. Heinrich, Shenglong Zhang, et al.precipitated genomic DNA was purified using spin column purification kit (Cell Signaling Technology; 14209) followed by PCR analysis with the primers targeting different promoters including SREBP2 promoter containing

(10) The formation and propagation of human Robertsonian chromosomesNatureNovember 27, 2025Leonardo Gomes de Lima, Andrea Guarracino, Sergey Koren, Tamara Potapova, Sean McKinney, Arang Rhie, Steven J. Solar, Chris Seidel, Brandon L. Fagen, Brian P. Walenz, et al.DNA purification was performed using a DNA purification with spin columns kit (Cell Signaling Technology, 14209). DNA concentration was assessed using the Qubit dsDNA HS kit for the Qubit Fluorometer. CUT&Tag library

(11) IL-3/STAT5/miR-155-5p axis supports stem-related pathway reprogramming in TNBCBreast Cancer ResearchNovember 3, 2025Alessandro Sarcinella, Sharad Kholia, Saveria Femminò, Massimo Cedrino, Marta Tapparo, Xiaofen Wen, Maria Rosaria De Miglio, Vincenzo Salemme, Andrea Scavuzzo, Matteo Poncina, et al.DNA purification was performed using DNA Purification Buffers and Spin Columns (Cell Signalling Technology #14209). Binding of phospho-STAT5 to the BIC promoter was quantified by qRT-PCR using the SimpleChIP Universal qPCR Master mix

(12) Nelfb promotes dermal white adipose tissue formation through RNA Polymerase II mediated adipogenic gene regulationDevelopmentOctober 15, 2025Samiksha Mahapatra, Julian Gomez, Uyanga Batzorig, Ye Liu, Celia Fernández-Méndez, Yifang Chen, George L. Sencrosslinks. DNA was purified using DNA purification buffers and spin columns kit (Cell Signaling Technology, #14209). Input samples were mixed with Extraction Buffer (Proteinase K+RNAse A) and incubated at 55°C for 1

(13) Coronary artery disease-associated variants regulate vascular smooth muscle cell gene expressionNature Cardiovascular ResearchOctober 7, 2025Nicolas Barbera, Lily Lei, Alexia Wallace, Faruk Erin, R. Noah Perry, Hester M. den Ruijter, Mete Civelekh. After MNase-induced cleavage, genomic DNA fragments were extracted using DNA spin columns (CST, #14209). Samples were prepared for sequencing with the DNA Library Prep Kit for Illumina (CST, #56795) combined

(14) Gtf2i-encoded transcription factor Tfii-i regulates myelination via Sox10 and Mbp regulatory elementsNature CommunicationsSeptember 26, 2025Gilad Levy, May Rokach, Inbar Fischer, Omri Kimchi-Feldhorn, Shiri Shoob, Ela Bar, Tali Rosenberg, Joanna Bartman, Hadar Parnas, Meitar Grad, et al.stand, and each sample underwent DNA cleaning and extraction using a DNA purification kit (catalog no. 14209, Cell Signaling). DNA was kept at −20 °C until library preparation. Library preparation and paired-end

(15) The muscle specific MEF2Dα2 isoform promotes muscle ketolysis and running capacity in miceEMBO ReportsSeptember 16, 2025Sushil Kumar, Xuan Ji, Hina Iqbal, Xiangnan Guan, Brittany Mis, Devanshi Dave, Suresh Kumar, Jacob Besler, Ranjan Dash, Zheng Xia, et al.for 6 h to reverse cross-links. DNA was purified using a spin column kit (Cell Signaling Technology 14209S). Quantitative PCR was performed with PowerTrack SYBR Green Master Mix (Sigma) on a CFX Opus 384 Real-Time PCR system

(16) Regulatory Role for Tumor Suppressor REST on Estrogen Receptor ( ESR1 ) Expression and Leiomyoma PathophysiologybioRxivSeptember 11, 2025Skylar G. Bird, Sumedha Gunewardena, Ashley S Cloud, Sornakala Ganeshkumar, Vargheese Mani Chennathukuzhiconfluency, both the cells and biopulverized tissue underwent standard ChIP kit protocol (Cell Signaling, #14209S, Cell Signaling Technology RRID:SCR_002071). Antibodies used for ChIP included rabbit IgG and H3XP as

(17) BRD2 Upregulation as a Pan-Cancer Adaptive Resistance Mechanism to BET InhibitionbioRxivSeptember 11, 2025Suyakarn Archasappawat, Juliette Jacques, EunJung Lee, Chang-il Hwangcolumns included in the kit according to the manufacturer’s instructions (Cell Signaling Technology, 14209S). Library preparation and sequencing Library preparation and sequencing for ChIP-seq and RNA-seq were

(18) NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial DamageInternational Journal of Molecular SciencesAugust 25, 2025Jingze Cong, Lihui Liu, Rui Shi, Mengting He, Yuchuan An, Xiaowei Feng, Xiaoyu Yin, Yingmin Li, Bin Cong, Weibo Shi3660S, Danvers, MA, USA), followed by addition of protein A/G magnetic beads (Cell Signaling Technology, 14209S, Danvers, MA, USA) to capture the antibody–protein–DNA complexes. After extensive washing to remove

(19) MYC Regulates a DNA Repair Gene Expression Program in Small Cell Carcinoma of the Ovary, Hypercalcemic TypeCancers (Basel)July 7, 2025James R. Evans, Jing Wang, Cinthia N. Reed, Joy H. Creighton, Kaylee B. Garrison, Abigail N. Robertson, Ashley Lira-Rivera, Diondre’ D. Baisden, William P. Tansey, Rafet Al-Tobasei, et al.free DNA. DNA was then purified for library preparation using the Cell Signaling DNA purification kit (14209). For IgG and MYC, the equivalent of three reactions was pooled during DNA purification per replicate.

(20) FGFR inhibitors promote the autophagic degradation of IFN-γ-induced PD-L1 and alleviate the PD-L1-mediated transcriptional suppression of FGFR3-TACC3 in non-muscle-invasive bladder cancerCell Death DisJuly 2, 2025Yu-Chen Lin, Cheng-Ying Chu, Tsung-Han Hsieh, Bo-Jyun Lin, Jing-Ping Liou, Yun Yen, Chun-Han Chena water bath, and the DNA fragments were de-crosslinked and purified using a DNA Purification kit (#14209, Cell Signaling Technology) according to the manufacturer’s instructions. For traditional PCR analysis,

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DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) from Cell Signaling Technology

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Cell Signaling Technology

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DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) from Cell Signaling Technology

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