Cytek Aurora™ CS System from Cytek Biosciences

Product Specs
ItemCytek Aurora^™ CS System
CompanyCytek Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberAurora CS
QuantityEA
Excitation Wavelength355 nm (20 mW), 405 nm (100 mW), 488 nm (50 mW), 561 nm (50 mW), 640 nm (80 mW)
Detector3 scatter detectors (FSC, SSC-B, SSC-V). And up to 64 high-resolution fluorescent detectors using Avalanche Photo Diodes (APDs)
Size(s)Four nozzles sizes including 70, 85, 100, and 130µm. All nozzles sizes are able to sort 6-ways into 1.5 and 5mL tubes, and 2-ways into 15 ml tubes. Able to sort into 96-well and 384-well plates, with the option to index sort.
ThroughputAble to sort 64 levels deep into the gating hiearchy and can sort up to 40 populations across 6 tubes; multiple populations can be sorted into any given tube.
Flow Rate10 to 80 µL/min
Dimensions75 x 57 x 65 cm
DetectionLasers with flat-top laser beam profile with a narrow vertical beam width; CWDM semiconductor array
Measuring Range64 fluorescence channels
CapacityTubes (5 mL, 15 mL)
Data Analysis25,000 events/sec

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Cytek Biosciences

47215 Lakeview Blvd.

Fremont, CA 94538

United States

Phone: (877) 922-9835

Email: products@cytekbio.com

Company Profile

Website: https://cytekbio.com/

(61) GFI1-driven transcriptional and epigenetic programs maintain CD8+ T cell stemness and persistenceNat ImmunolMay 15, 2025M. Zeeshan Chaudhry, Evelyn Chen, Hiu On Man, Aneesha Jones, Renae Denman, Huiyang Yu, Qiutong Huang, Adrian Ilich, Jaring Schreuder, Severine Navarro, et al.and dead cells. Cells were then flow-cytometrically sorted on a BD FACSAria II (BD Biosciences) or Aurora CS Cell Sorter (Cytek Biosciences), and 1 × 104 cells were transferred into each recipient. The secondary

(62) Monocytes can efficiently replace all brain macrophages and fetal liver monocytes can generate bona fide SALL1+ microgliaImmunityMay 13, 2025Jonathan Bastos, Carleigh O'Brien, Mónica Vara-Pérez, Myrthe Mampay, Lynn van Olst, Liam Barry-Carroll, Daliya Kancheva, Sophia Leduc, Ayla Line Lievens, Leen Ali, et al.eBeads (Thermofisher, 01-2222-42). Flow cytometry experiments were performed on the LSRFortessa (BD) and Aurora CS 5 laser system (Cytek). FACS sorting was performed on the FACSAria III cell sorter (BD), FACSMelody cell sorter

(63) Human skeletal development and regeneration are shaped by functional diversity of stem cells across skeletal sitesCell Stem CellMay 1, 2025Thomas H. Ambrosi, Sahar Taheri, Kun Chen, Rahul Sinha, Yuting Wang, Ethan J. Hunt, L. Henry Goodnough, Matthew P. Murphy, Holly M. Steininger, Malachia Y. Hoover, et al.CD164 (BioLegend, Cat#324808), and CD73 (BioLegend, Cat#344016). A FACS Aria II (BD Biosciences) or AuroraCS (CYTEK) was used for flow cytometric analysis and sorting of hSSCs. Gating was established with IgG

(64) ER-phagy mediates the anti-tumoral synergism between HDAC inhibition and chemotherapyCell Commun SignalApril 26, 2025Felix J. Gössl, Pierfrancesco Polo, Frederik Helmprobst, André Menzenbach, Alexander Visekruna, Thomas M. Gress, Till Adhikary, Matthias Lauthμg/ml; 1.8 μM) for the mKeima-reporter. The in-house FACS Core Facility sorted these cells using an Aurora CS (Cytek). Sorted cells were cultured in complete growth medium containing the respective antibiotic. Flow cytometry

(65) A novel acridine flow cytometry marker to track post‐transfusion amustaline/glutathione pathogen‐reduced red blood cell survival in sickle cell disease patientsTransfusionApril 18, 2025Richard J. Benjamin, John P. Pitman, Christopher Karim, Shengnan Huang, Anna Erickson, Laurence Corash, Nina Mufti, John D. Roback, Patricia E. Zerra, Ross M. Fasano, et al.deglycerolized in patient‐specific batches at Cerus Corporation and analyzed in triplicate on a Cytek Aurora CS flow cytometer (Cytek, Fremont, CA). Triplicate results were averaged and background negative‐Control

(66) Translation of unspliced retroviral genomic RNA in the host cell is regulated in both space and timeJ Cell BiolApril 7, 2025Felipe Leon-Diaz, Célia Chamontin, Sébastien Lainé, Marius Socol, Edouard Bertrand, Marylène Mougel100 µg/ml. 1 wk later, transfected cells were sorted by fluorescence-activated cell sorting (Cytek Aurora CS; FACS). While maintaining hygromycin pressure, 1.5 × 106 cells were seeded on a 10-cm plate and transfected

(67) Orchestrating T and NK cells for tumor immunotherapy via NKG2A-targeted delivery of a de novo designed IL-2Rβγ agonistDrug DelivApril 2, 2025Jie Chen, Enhui Ren, Ze Tao, Hongyu Lu, Yunchuan Huang, Jing Li, Yuzhe Chen, Zhuo Chen, Tianshan She, Hao Yang, et al.effector/memory T cells, respectively (Chen et al., 2020). Samples were analyzed on BD LSRfortessa (BD, NJ) or Cytek® Aurora CS (Cytek®, CA) or BD FACADiscover S8 (BD, NJ). Data were processed using Flow jo 10.1 software and GraphPad

(68) Kupffer cell and recruited macrophage heterogeneity orchestrate granuloma maturation and hepatic immunity in visceral leishmaniasisNat CommunApril 1, 2025Gabriela Pessenda, Tiago R. Ferreira, Andrea Paun, Juraj Kabat, Eduardo P. Amaral, Olena Kamenyeva, Pedro Henrique Gazzinelli-Guimaraes, Shehan R. Perera, Sundar Ganesan, Sang Hun Lee, et al.CD11int CD64+ macrophages or CD45.2+ F4/80hi CD11int CD64+CLEC4F-TIM-4+ KCs were sorted using the Cytek® Aurora CS sorter and collected for RNA extraction and library preparation using the Chromium Next GEM Single Cell

(69) MAIT cell deficiency exacerbates neuroinflammation in P301S human tau transgenic miceJ NeuroinflammationMarch 20, 2025Yuanyue Zhang, Zhi Yang, Na Jiang, Xiaosheng Tan, Peng Jiang, Gaoyuan Cao, Qi Yang4-laser LSRII (BD Biosciences) or a 4-laser Cytek Aurora, and cell sorting was performed on a Cytek Aurora CS cell sorter. Immunofluorescence staining For immunofluorescence staining, mice were first perfused

(70) Machine learning-based prediction reveals kinase MAP4K4 regulates neutrophil differentiation through phosphorylating apoptosis-related proteinsPLoS Comput BiolMarch 17, 2025Guihua Wang, Dan Zhang, Zhifeng He, Bin Mao, Xiao Hu, Li Chen, Qingxin Yang, Zhen Zhou, Yating Zhang, Kepan Linghu, et al.washing to remove excess antibodies, BMCs, splenocytes, and peripheral blood cells were analyzed using an Aurora CS Flow Cytometer (CYTEK), and Hl-60 cells were analyzed using an Attune Nxt (Life Technologies) flow cytometer.

(71) Cells that survive acute SARS-CoV-2 infection contribute to inflammation and lung regeneration in micemBioMarch 12, 2025Pan R, Meyerholz DK, Perlman S.Cytofix/Cytoperm Solution (BD Biosciences) for 24 h at 4°C. All flow cytometric data were acquired on an Aurora CS system (Cytek Biosciences). The following antibodies were used for flow cytometry: CD45-eFluor605 (Invitrogen,

(72) Transcriptional reprogramming triggered by neonatal UV radiation or Lkb1 loss prevents BRAFV600E-induced growth arrest in melanocytesOncogeneMarch 8, 2025Kimberley McGrail, Paula Granado-Martínez, Roberto Orsenigo, Ginevra Caratù, Paula Nieto, Holger Heyn, Berta Ferrer, Javier Hernández-Losa, Eva Muñoz-Couselo, Vicenç García-Patos, et al.green fluorescence (expressing BRAFV600E and UPP1, CHTF18, or BNC1) were sorted by flow cytometry using Aurora CS spectral technology (Cytek). The raw sequencing data (fastq format) were processed using 10× Genomics

(73) Apolipoprotein E is a marker of all chondrocytes in the growth plate resting zoneBone ResMarch 3, 2025Joe Kodama, Takeshi Oichi, Kevin J. Wilkinson, Joshua M. Abzug, Takashi Kaito, Motomi Enomoto-Iwamoto, Masahiro Iwamoto, Satoru Otsurusurface markers and subjected to flow cytometrical analysis or cell sorting using an Aurora 4 UV or an Aurora CS (Cytek Biosciences): CD45-APC (#103112, BioLegend), CD45-BV421 (#103134, BioLegend), CD31-BV421 (#102424,

(74) Ultra-high dose rate radiotherapy overcomes radioresistance in head and neck squamous cell carcinomaSignal Transduct Target TherMarch 3, 2025Hong-Shuai Li, Ruo Tang, Hua-Shan Shi, Zi-Jian Qin, Xiao-Yang Zhang, Yun-Fei Sun, Zhi-Gong Wei, Chao-Fan Ma, Liu Yang, Ye Chen, et al.Biotechnology, China). Mouse immune cells, BMDMs, and SPTCs samples were analyzed using spectral flow cytometry (Aurora CS, Cytek, USA) with a panel of antibodies including Ms CD16/CD32 Pure 2.4G2, APC-Cy7 Rat Anti-Mouse CD5

(75) Myelin-reactive CD8+ T cells influence conventional dendritic cell subsets towards a mature and regulatory phenotype in experimental autoimmune encephalomyelitisJournal of NeuroinflammationFebruary 28, 2025Mohit Upadhye, Connor R. Wilhelm, Kai J. Rogers, Chakrapani Vemulawada, Nicholas Borcherding, Alexander W. Boyden, Kevin L. Legge, Nitin J. Karandikarmechanisms. cDC subsets, cDC1 and CD11b+ cDC were sorted using Fluorescence-Activated Cell Sorting (Cytek Aurora CS) from PLP-immunized mice 10 days post-immunization. For the trans-well assay, CD11b+ cDC (80,000) were

(76) Characterizing the antibody response to amustaline/glutathione pathogen‐reduced red blood cellsTransfusionFebruary 1, 2025Christopher Karim, Anil Panigrahi, Ronald G. Pearl, Neel R. Sodha, Thomas M. Beaver, J. Peter R. Pelletier, Gregory A. Nuttall, T. Brett Reece, Anna Erickson, Teresa Hedrick, et al.of the study. 6 PRRBC surface‐bound acridine and surface‐bound human IgG were analyzed on a Cytek Aurora CS flow cytometer (Cytek, Fremont, CA). Briefly, surface‐bound human IgG analysis utilized a commercial

(77) Monoallelic expression can govern penetrance of inborn errors of immunityNatureJanuary 30, 2025O’Jay Stewart, Conor Gruber, Haley E. Randolph, Roosheel Patel, Meredith Ramba, Enrica Calzoni, Lei Haley Huang, Jay Levy, Sofija Buta, Angelica Lee, et al.sorted for Live+CD3+CD4+ or Live+CD3+CD8+ into 96-well v-bottom plates (Invitrogen) using the Cytek Aurora CS. Each well of the 96-well plate contained 10,000 CD3-autologous PBMC irradiated feeder cells in 50 μl

(78) Multivalent S2 subunit vaccines provide broad protection against Clade 1 sarbecoviruses in female miceNat CommunJanuary 7, 2025Peter J. Halfmann, Raj S. Patel, Kathryn Loeffler, Atsuhiro Yasuhara, Lee-Ann Van De Velde, Jie E. Yang, Jordan Chervin, Chloe Troxell, Min Huang, Naiying Zheng, et al.in 1 × PBS supplemented with 0.2% BSA and 2 mM Pierce biotin for downstream cell sorting using the Aurora CS Cell Sorter (Cytek Biosciences). BB220 + /IgM-/IgD-/antigen-PE-positive cells were sorted as probe positive. Single-cell RNA-seq

(79) Obesity prolongs the pro-inflammatory response and attenuates bone healing on titanium implantsActa BiomaterialiaJanuary 1, 2025Derek Avery, Lais Morandini, Luke Sheakley, Asmaa Alajmi, Leah Bergey, Henry J. Donahue, Rebecca K. Martin, Rene Olivares-Navarretecontrols and fluorescent minus one, as previously described [27]. The samples were analyzed using a Cytek Aurora CS Cytometer (Cytek Biosciences, Fremont, CA). A minimum of 200,000 events were collected per sample, and

(80) Reduced thymic IL-4 impairs negative T cell selection in nonobese diabetic miceJournal of Clinical InvestigationDecember 2, 2024Alexis N. Cattin-Roy, Kimberly G. Laffey, Luan B. Le, Adam G. Schrum, Habib Zaghouanisample. Cell sorting Cell sorting was performed on a Beckman Coulter MoFlo XDP cell sorter or on a Cytek Aurora CS. Cell purity was routinely checked, and only sorts with a purity of > 95% were used in this study. ETPs. ETPs

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Cytek Aurora^™ CS System

Cytek Aurora^™ CS System from Cytek Biosciences

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Cytek Biosciences

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Cytek Aurora^™ CS System from Cytek Biosciences

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