Invitrogen™ Bigfoot™ Cell Sorter from Thermo Fisher Scientific

Product Specs
ItemInvitrogen™ Bigfoot™ Cell Sorter
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberBigfoot Spectral
QuantityEA
Speed> 70,000 eps with all parameters enabled*
Dimensions39 x 39 x 67 in
Power100 to 240VAC 50/60Hz
Excitation Wavelength349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm, 594 nm, 640 nm, 785 nm

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sortingSTAR ProtocApril 24, 2026Hemanta Sarmah, Grazia Iannello, Ryan Wantroba, Christopher Wu, Juan Idiarte, Adonary Munoz, Olivier Déry, Elena Rubio de la Torre, Ekaterina Lebayle, Serena Seminara, et al.WOLF G2®) (Figure 1). Alternative instruments, such as the BD FACSAria, BD Discovery S8, ThermoFisher Bigfoot Spectral Cell Sorter, or other advanced automated platforms may also be used. To optimize post-sorting survival

(2) Ciliated Cells Drive Critical STING-Mediated Tumor Suppression in the Fallopian Tube EpitheliumCancer ResApril 22, 2026Jose A. Colina, Maria Sol Recouvreux, Alexander M Sobeck, Benjamin K. Johnson, Yinzhi Lin, Sreeja C. Sekhar, Rita A. Avelar, Gabriela Rivera-Gonzalez, Yali Zhai, Harini Ram, et al.(ThermoScientific Cat# V13241B), according to the manufacturer’s instructions. Flow cytometry was performed using a Bigfoot Spectral Cell Sorter, and GFP-positive cells were gated to exclude MOE STING-OE cells. Apoptosis was quantified

(3) A structure-informed deep learning framework for modeling TCR-peptide-HLA interactionsbioRxivApril 11, 2026Kai Cao, Rui Li, Martin Strazar, Eric M. Brown, Phuong N.U. Nguyen, Marie-Madlen Pust, Jihye Park, Daniel B. Graham, Orr Ashenberg, Caroline Uhler, et al.were stained with PE-conjugated anti-human TCRαβ (BioLegend; 306708). TCRαβ− cells were sorted with a Bigfoot™ Spectral Cell Sorter (Thermo Fisher Scientific). Lentiviral vector production and transduction HEK293T cells

(4) CMAH-Targeted Knock-In of Inducible Heme Oxygenase-1 and Constitutive CD47 in GGTA1-Knockout Pigs for XenotransplantationXenotransplantationMarch 30, 2026Haesun Lee, Sang Eun Kim, Won Kil Lee, Miae Oh, Seunghoon Lee, Jin-Gu No, Mi-Ryung Park, Seokho Kim, Min Hwa Do, Keon Bong Ohallophycocyanin APC‐conjugated anti‐CD47 antibody (Invitrogen). Flow cytometry was performed using a Bigfoot Spectral Cell Sorter (Invitrogen), and data were analyzed using FlowJo10.10.0 software (FlowJo LLC, Ashland,

(5) Genome-scale functional mapping of the mammalian whole brain with in vivo Perturb-seqbioRxivMarch 20, 2026Tuo Shi, Maria Korshunova, Seoyeon Kim, David DeTomaso, Xinhe Zheng, Lavanya Vishvanath, Thokozile Nyasulu, Nhan Huynh, Alexander Sun, Patrick C. Thompson, et al.and 0.2 U/μL RNase Inhibitor (Fisher #NC1081844) using the MA900 (Sony), MoFlo Astrios (Beckman), or Bigfoot Spectral Cell sorter (Thermo Fisher) with 100 μm sorting chips. Sorted nuclei were then resuspended in 1x Quenching

(6) Structural insights into antibody responses against influenza A virus in its natural reservoirbioRxivMarch 13, 2026Huibin Lv, Walter N. Harrington, Wenkan Liu, Dalia Naser, Yang Wei Huan, Emma Thames, Pradeep Chopra, Tossapol Pholcharee, Patrick Seiler, Edgardo Ayala, et al.Single BB515+PE+ B cells were sorted into 5 mL tubes each containing 2 mL of 1× PBS with 2% FBS using a Bigfoot Spectral Cell Sorter. The sorted cells were counted by using 0.4% (w/v) trypan blue stain solution (Thermo Fisher Scientific

(7) aGPCR-HEK: A Stable High-Expression Inducible Mammalian Cell Expression System for Adhesion GPCR Structural Biology ApplicationsBIO-PROTOCOLMarch 5, 2026David M. Favara, Christopher G. TateLife Technologies EVOS FL microscope (Life Technologies, catalog number: AMF4300) 8. FACs cell sorter: Invitrogen Bigfoot Cell Sorter (Invitrogen, catalog number: PL00304) 9. Flow cytometer (Sony, model: ID7000 Spectral Cell Analyzer) 10.

(8) LYPLAL1 Rare Loss-of-Function Variants in Humans and Deletion in Human Hepatoma Cells Protect Against MASLDJournal of Lipid ResearchMarch 3, 2026Rawdat Hussain, Chinmay Raut, Ponnandy Prabhu, Akram Irshaid, Yash Hegde, Yue Chen, Asmita Pant, Antonino Oliveri, Xiaochao Wei, Lishi Yin, et al.#CMAX00003). After growing for a week, single cells were sorted into 96 well plates with a ThermoFisher Bigfoot Spectral Cell Sorter. Knockout of LYPLAL1 was confirmed via sequencing along with RT-qPCR and Western blot analysis. Overexpression

(9) Characterization of the clonal hierarchy and immunophenotype of PTPN11 mutations in acute myeloid leukemiaJCI InsightFebruary 23, 2026Sydney Fobare, Chia Sharpe, Kate Quinn, Kinsey Bryant, Linde A. Miles, Robert L. Bowman, Carolyn Cheney, Casie Furby, Marissa Long, Kaytlynn Fyock, et al.into a secondary NCG recipient. For the LSK and lineage+ engraftment, splenocytes were sorted on the BigFoot Spectral Cell Sorter (Thermo Fisher Scientific), and LSKs or lineage+ populations (total CD45+, CD11c+ [CD45+CD3–CD19–Nk1.1–Ly6G–CD11c+]

(10) Coronaviruses reprogram the tRNA epitranscriptome to favor viral protein expressionNature CommunicationsFebruary 19, 2026Elena Muscolino, Mireia Puig-Torrents, Jaime Buigues Bisquert, Diogo Correa Mendonca, Marc Talló-Parra, Gemma Perez-Vilaro, Omar Caño-Prades, Gavin R. Meehan, Karen Kerr, Vanessa Herder, et al.were washed, detached with phenol-free trypsin, and immediately analyzed by flow cytometry using a BigFoot Spectral Cell Sorter (ThermoFisher Scientific). Fluorescence was collected in the FITC channel (488), and ROS

(11) A Multimodal Single-Cell Epigenomic and 3D Genome Atlas of the Human Basal GangliabioRxivFebruary 17, 2026Wubin Ding, Amit Klein, Cindy Tatiana Báez-Becerra, Jonathan A. Rink, Anna Bartlett, Qiurui Zeng, Ruoxuan Wang, Rosa Gomez Castanon, Joseph R. Nery, Emma Osgood, et al.1:1000 for 5 min at 4°C.Processed nuclei were brought on ice for sorting into 384-well plates using a Bigfoot Spectral Cell Sorter (Thermo Fisher Scientific, cat. no. PL00299) with a 100 μm nozzle, sheath pressure of 30

(12) Decidual CD4 Effector Memory and Resident Memory T Cells Respond to Cord Blood Allo‐AntigensAmerican Journal of Reproductive ImmunologyFebruary 1, 2026Zachary T. Koenig, Shruti Eswar, Sayaka Tsuda, Nathan Schuldt, Claire A. Chougnet, Braxton Forde, Sandra Andorf, Tamara Tilburgsand permeabilized using the eBiosciences FOXP3 kit (eBiosciences). Cell sorting was performed using a Bigfoot spectral cell sorter or a BD S6/S6SE cell sorter. For immune phenotyping, data acquisition was done using a 5‐laser

(13) CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistanceCell Reports MedicineJanuary 20, 2026Alex Watterson, Gabriele Picco, Vivien Veninga, Youhani Samarakoon, Chiara M. Cattaneo, Sara F. Vieira, Emre Karakoc, Shriram Bhosle, Thomas W. Battaglia, Sarah Consonni, et al.containing their respective advanced media (advanced-RPMI and advanced-DMEM; Thermo Fisher) using a Bigfoot Spectral Cell Sorter (Thermo Fisher). Clones were expanded and assessed for genomic JAK1 or JAK2 KO by PCR of

(14) Distinct p21 dynamics drive alternative routes to whole-genome duplication through a common CDK4/6-dependent polyploid G0 statebioRxivJanuary 18, 2026Yian Yang, Anuraag Bukkuri, Dante Poe, Janet McLaughlin, Sihan Hao, Daniel D. Brown, Katarzyna M. Kedziora, Wayne Stallaertstrainer to remove aggregates, and maintained on ice until analysis. Sorting was performed using the Bigfoot Spectral Cell Sorter (ThermoFisher Scientific) under sterile conditions, and sorted cells were collected into

(15) AKAP95 condensates regulate transcription and can be targeted in MLL-fusion driven oncogenesisNature CommunicationsJanuary 7, 2026Xiang Yan, Yongkun Luan, Yi Zhu, You Cai, Fanghua Ye, Liangchun Yang, Kaibo Zhu, Jing Hu, Shuangzi Luo, Jiazheng Lin, et al.(Life Technologies). After 4 days of selection against puromycin, cells were sorted on GFP signals on Bigfoot spectral cell sorter (Thermo Fisher Scientific). About 8000 GFP-positive cells were seeded in 6-well plates.

(16) Bmi-1 inhibition sensitizes head and neck cancer stem cells to cytotoxic chemotherapyTranslational OncologyJanuary 1, 2026Alexandra E. Herzog, Shirley Zheng, Kristy A. Warner, Jaqueline V. Vanini, Ritu Somayaji, Madelynn R. Johnson, Zhaocheng Zhang, Peter J. Polverini, Rogério M Castilho, Alexander T. Pearson, et al.stained with DAPI (Molecular Probes, Eugene, OR, USA). All flow cytometry analysis was conducted by the Bigfoot Spectral Cell Sorter (Invitrogen). Results were analyzed with FlowJo v10.10 software (BD Life Sciences, Franklin

(17) Treatment of Huntington’s disease with a pan-HTT-targeting CRISPR nucleasebioRxivDecember 24, 2025Katherine Tan, Daniela Del Bosque Siller, Alisha Y. Xiong, Anna X.A. Wang, Tristan X. McCallister, Sreya Mummadi, Luke A. St John, Tae Kyu Lee, Annika Carrillo, Daniel G. Renshaw, et al.polystyrene tubes with a 35 μm nylon mesh cell strainer snap cap (Falcon) and then sorted using the Bigfoot Spectral Cell Sorter (Thermo Fisher Scientific; Roy J. Carver Biotechnology Center, University of Illinois Urbana-Champaign).

(18) Fluoride triggers lysis in Streptococcus mutans by inhibition of the Clp protease complex, leading to an unabated competence cascadeProceedings of the National Academy of SciencesDecember 16, 2025Aditya Banerjee, Sophie Pickelner, Faith Smith, Livia M. A. Tenuta, Randy B. Stockbridge(FACS) Cells were stained with AnnexinV-FITC and propidium iodide (PI) (Invitrogen, USA) and sorted using a Bigfoot Spectral Cell Sorter (Thermo Fisher Scientific, USA) with λex 561 and 488 nm, and emission band-passes 625±15

(19) Competition between Der1 and ERAD-M substrates controls Hrd1 complex functionProceedings of the National Academy of SciencesDecember 9, 2025Jennifer E. Russ, Brian G. Peterson, Sophia Taylor, Ryan D. Baldridgetemperature, pelleted, washed in PBS, resuspended in PBS containing 1µM SytoxBlue, and sorted using a Bigfoot Spectral Cell Sorter (Invitrogen (formally Propel Labs)). Forward and side measurements were analyzed on the

(20) Multiplex neurodegeneration proteotoxicity platform reveals DNAJB6 promotes non-toxic FUS condensate gelation and inhibits neurotoxicityNature CommunicationsNovember 21, 2025Samuel J. Resnick, Seema Qamar, Pushya Krishna, Vladislav Korobeynikov, Hannes Ausserwoger, Alyssa Miller, Pietro Esposito, Juan A. Varela, Jenny Sheng, Lei Haley Huang, et al.during the outgrowth process. After 2 weeks, single cells were sorted into 96 well plates using the Bigfoot Spectral Cell Sorter (Thermo). To gate on single cells, forward scatter and side scatter were used to isolate

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