BD FACSymphony™ S6 Cell Sorter from BD Biosciences

Product Specs
ItemBD FACSymphony™ S6 Cell Sorter
CompanyBD Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberFACSymphony S6
QuantityEA
Detection50 detection parameters (including FSC and SSC), featuring decagon arrays for up to 10 parameters on a single laser line. Select and configure up to a maximum of 10 lasers from various wavelengths with multiple power ratings
Absorbance / Excitation355 nm, 375 nm, 405 nm, 420 nm, 445 nm, 458 nm, 460 nm, 473 nm, 488 nm, 505 nm, 514 nm, 532 nm, 552 nm, 561 nm, 568 nm, 588 nm, 592 nm, 628 nm, 637 nm, 640 nm, 647 nm, 660 nm, 685 nm, 730 nm, 785 nm, 980 nm
Sample Type5.0-mL polystyrene and polypropylene tubes, optional high throughput sampler to acquire samples from a 96- or 384-well microtiter plate.
SensitivityFluorescence sensitivity may vary dependent on the combination of laser and filter selections
TypeCell Soreter
Plate Formats1.5-, 2.0- and 5.0-mL tubes; optional high througput sampler to acquire samples from a 96- or 384-well microtiter plate.

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BD Biosciences

2350 Qume Drive

San Jose, California 95131

United States

Company Profile

Website: http://www.bdbiosciences.com/

(1) Omicron variants escape the persistent SARS-CoV-2-specific antibody response in 2-year COVID-19 convalescents regardless of vaccinationEmerging Microbes & InfectionsDecember 31, 2023Miao Wang, Bing Zhou, Qing Fan, Xinrong Zhou, Xuejiao Liao, Jingyan Lin, Zhenghua Ma, Jingke Dong, Haiyan Wang, Xiangyang Ge, et al.using the LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen). Flow cytometric data were acquired on an FACSymphony S6 flow cytometer (BD Biosciences) and analyzed using FlowJo software (TreeStar). SARS-CoV-2 pseudovirus-based

(2) Prior viral infection primes cross-reactive CD8+ T cells that respond to mouse heart allograftsFront ImmunolDecember 8, 2023M. Eyad Khorki, Tiffany Shi, Eileen E. Cianciolo, Ashley R. Burg, P. Chukwunalu Chukwuma, Jennifer L. Picarsic, Mary K. Morrice, E. Steve Woodle, Jonathan S. Maltzman, Autumn Ferguson, et al.after transplantation. Hearts were digested as described above and pool together for cell staining. The FACSymphony S6 (BD Biosciences) was used to sort for the following 6 CD8+ T cell populations: CD44hiGP33+GFP+, CD44hiGP33+GFP-,

(3) Interaction dynamics between innate and adaptive immune cells responding to SARS-CoV-2 vaccination in non-human primatesNat CommunDecember 2, 2023Chaim A. Schramm, Damee Moon, Lowrey Peyton, Noemia S. Lima, Christian Wake, Kristin L. Boswell, Amy R. Henry, Farida Laboune, David Ambrozak, Samuel W. Darko, et al.buffer (Qiagen) with 1% β-mercaptoethanol for sequencing by SmartSeq. All sorts were performed using a BD FACSymphony S6 Cell Sorter (BD Biosciences) with BD FACSDiva Software version 9.5.1 (BD Biosciences) and data were analyzed using

(4) Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular moleculesNature CommunicationsNovember 17, 2023Shodai Komatsu, Hirohisa Ohno, Hirohide Saitopuromycin (Invivogen, #ant-pr-1). The fluorescence-positive cells were then sorted and enriched using FACSymphony S6 cell sorter (BD Biosciences). The sorted cells were maintained in the medium containing 0.5 µg/mL puromycin. Luciferase

(5) Tau accumulation in degradative organelles is associated to lysosomal stressScientific ReportsOctober 21, 2023Ester Piovesana, Claudia Magrin, Matteo Ciccaldo, Martina Sola, Manolo Bellotto, Maurizio Molinari, Stéphanie Papin, Paolo Paganetti40 μm cell strainer (15-1040-2, Biologix) and resuspended in 1 mL cold MACS buffer before sorting (FACSymphony S6, BD Biosciences). Live cells were gated using DAPI channel (ex: 350 nm, em: 460 nm). Successively, selected

(6) An engineered cell line with a hRpn1-attached handle to isolate proteasomesThe Journal of biological chemistryAugust 1, 2023Negi H, Osei-Amponsa V, Ibrahim B, Evans CN, Sullenberger C, Loncarek J, Chari R, Walters KJ.treated with DNA-staining DAPI (D3571; Thermo Fisher Scientific, Inc) prior to sorting by FACS with a BD FACSymphony S6 Cell Sorter (BD Sciences). Forward scatter (FSC)-area and side scatter (SSC)-area settings were used to gate for

(7) Cysteine-rich intestinal protein 1 is a novel surface marker for human myometrial stem/progenitor cellsCommunications BiologyJuly 3, 2023Emmanuel N. Paul, Tyler J. Carpenter, Sarah Fitch, Rachael Sheridan, Kin H. Lau, Ripla Arora, Jose M. Teixeiradiscrimination. Cells were sorted by the flow cytometry core at Van Andel Research Institute (VARI) using a FACSymphony S6 cytometer (BD Biosciences) and analyzed with FlowJo Software (BD Biosciences, version 10.8.1). The side

(8) A viral pan-end RNA element and host complex define a SARS-CoV-2 regulonNature CommunicationsJune 9, 2023Debjit Khan, Fulvia Terenzi, GuanQun Liu, Prabar K. Ghosh, Fengchun Ye, Kien Nguyen, Arnab China, Iyappan Ramachandiran, Shruti Chakraborty, Jennifer Stefan, et al.without IFN-γ (500 U/ml) for 24 h. Treated and untreated GFP+ cells were collected by sorting using the BD FACSymphony™ S6 Cell Sorter (BD Biosciences). RNA Immunoprecipitation (RIP)-qPCR GFP+ 293 T cells were lysed in ~300 μL IP buffer

(9) The capture of extracellular vesicles endogenously released by xenotransplanted tumours induces an inflammatory reaction in the premetastatic nicheJournal of Extracellular VesiclesMay 17, 2023Laurence Blavier, Rie Nakata, Paolo Neviani, Khounish Sharma, Hiroyuki Shimada, Aitor Benedicto, Irina Matei, David Lyden, Yves A. DeClerckdetected with an antibody against desmin). Data acquisition was done on a LSRII flow cytometer and a FACSymphony S6 Cell Sorter (BD Biosciences). A reading <10−4 events was considered below the limit of the sensitivity

(10) Engineered cytokine/antibody fusion proteins improve delivery of IL-2 to pro-inflammatory cells and promote antitumor activitybioRxivMay 9, 2023Elissa K. Leonard, Jakub Tomala, Joseph R. Gould, Michael I. Leff, Jian-Xin Lin, Peng Li, Mitchell J. Porter, Eric R. Johansen, Ladaisha Thompson, Shanelle D. Cao, et al.induced. The final three rounds of selection were performed by fluorescence-activated cell sorting (FACS) on a FACSymphony S6 cell sorter (Becton Dickinson), using decreasing amounts of biotinylated IL-2 in the presence of a large

(11) SEACells infers transcriptional and epigenomic cellular states from single-cell genomics dataNature BiotechnologyMarch 27, 2023Sitara Persad, Zi-Ning Choo, Christine Dien, Noor Sohail, Ignas Masilionis, Ronan Chaligné, Tal Nawy, Chrysothemis C. Brown, Roshan Sharma, Itsik Pe’er, et al.for 20 minutes at 4 °C. Cells were washed twice with CSB before fluorescence-activated cell sorting (FACSymphony S6, BD Biosciences) where CD3− cells were collected. Sorted cells were concentrated, and count and viability

(12) Heterologous SARS-CoV-2 spike protein booster elicits durable and broad antibody responses against the receptor-binding domainNature CommunicationsMarch 15, 2023Tomohiro Takano, Takashi Sato, Ryutaro Kotaki, Saya Moriyama, Shuetsu Fukushi, Masahiro Shinoda, Kiyomi Kabasawa, Nagashige Shimada, Mio Kousaka, Yu Adachi, et al.Biosciences, #612798). The cells were washed with B cell medium, resuspended in the medium, and analyzed using FACSymphony S6 (BD Biosciences) and FACSDiva v.9.1.2 software (BD Biosciences) or Symphony A3 cytometer (BD Biosciences)

(13) Distinct Tumor Necrosis Factor Alpha Receptors Dictate Stem Cell Fitness versus Lineage Output in Dnmt3a-Mutant Clonal HematopoiesisCancer DiscoveryDecember 2, 2022Jennifer M. SanMiguel, Elizabeth Eudy, Matthew A. Loberg, Kira A. Young, Jayna J. Mistry, Kristina D. Mujica, Logan S. Schwartz, Timothy M. Stearns, Grant A. Challen, Jennifer J. Trowbridgemarker mix, and a viability stain. Stained cells were sorted using a FACSAria II (RRID:SCR_018091) or a FACSymphony S6 Sorter (BD Biosciences), or analyzed on a FACSymphony A5 or LSR II (RRID:SCR_002159) with Diva software

(14) Differential expression of CD8 defines phenotypically distinct cytotoxic T cells in cancer and multiple sclerosisClinical and translational medicineDecember 1, 2022Burkard T, Herrero San Juan M, Dreis C, Kiprina A, Namgaladze D, Siebenbrodt K, Luger S, Foerch C, Pfeilschifter JM, Weigert A, et al.BD Biosciences, Heidelberg, Germany, RRID: AB_1645581) into CD8Low and CD8High T cell subsets with the FACSymphony S6 Cell sorter (BD Biosciences, San Diego, CA). A general gating strategy for the CD8+ T lymphocytes (Supplementary

(15) Combinatorial depletions of G-protein coupled receptor kinases in immune cells identify pleiotropic and cell type-specific functionsFrontiers in ImmunologyNovember 14, 2022Katharina M. Glaser, Teresa K. Tarrant, Tim LämmermannCD45+, B220+, CD23+) and marginal zone B cells (Lin-, CD45+, B220+, CD21+, CD23-) were sorted on a FACSymphony™ S6 or FACSAria™ Fusion flow cytometer (BD). Angiogenesis µ-slides (Ibidi) were coated overnight with 2

(16) Nanoparticle‐Based Follistatin Messenger RNA Therapy for Reprogramming Metastatic Ovarian Cancer and Ameliorating Cancer‐Associated CachexiaSmallNovember 1, 2022Tetiana Korzun, Abraham S. Moses, Jeonghwan Kim, Siddharth Patel, Canan Schumann, Peter R. Levasseur, Parham Diba, Brennan Olson, Katia Graziella De Oliveira De Oliveira Rebola, Mason Norgard, et al.Waltham, MA, USA) and GFP (APC Anti-GFP antibody, Abcam, Cambridge, UK). Flow cytometry was performed on BD FACSymphony S6 cell sorter (BD, Franklin lakes, NJ, USA) using laser excitation wavelength of 561 nm and 628 nm for HLA-ABC and

(17) Assessment of Fcγ receptor-dependent binding of influenza hemagglutinin vaccine-induced antibodies in a non-human primate modeliScienceOctober 21, 2022Masuta Y, Takahama S, Nogimori T, Moriyama S, Takahashi Y, Yamamoto T.treated with the serum for 4 h. Subsequently, the classical monocytes were sorted from the PBMCs using a FACSymphony S6 (BD Biosciences) based on the gating strategy shown in Figure S3A. mRNA was isolated using an RNeasy

(18) Long-primed germinal centres with enduring affinity maturation and clonal migrationNatureSeptember 29, 2022Jeong Hyun Lee et al.sorted on a FACSFusion (BD Biosciences); those from groups 2 and 3 were either acquired or sorted on a FACSymphony S6 (BD Biosciences). Indexed V(D)J, Feature Barcode and GEX libraries of sorted LN FNA samples were prepared

(19) Epigenetic reprogramming leads to down regulation of CD4 and functional changes in African green monkey memory CD4+ T cellsThe Journal of ImmunologyJuly 15, 2022Andrew R. Rahmberg, Tovah E. Markowitz, Joseph C. Mudd, Vanessa Hirsch, Jason M. Brenchleyand resuspended in complete RPMI with 10% FBS. For each population, 30,000 cells were sorted using a FACSymphony S6 cell sorter (BD). ATACseq processing: Tagmentation was performed by pelleting sorted cells and resuspending

(20) A shared disease-associated oligodendrocyte signature among multiple CNS pathologiesNature neuroscienceJuly 1, 2022Kenigsbuch M, Bost P, Halevi S, Chang Y, Chen S, Ma Q, Hajbi R, Schwikowski B, Bodenmiller B, Fu H, et al.except for enzymatic digestion. Single-cell sorting Cell populations were sorted using FACS-AriaIII or FACSymphony-S6 and FACS DIVA software (BD Biosciences, San Jose, CA). For the sorting of all the non-immune populations,

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