pNLF1-N [CMV/Hygro] Vector from Promega

Product Specs
ItempNLF1-N [CMV/Hygro] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1351
Quantity20microg
Nucleic Acid TypeReporter Vectors
Expression System (standardized)Mammalian
Vector NamepNLF1-N [CMV/Hygro]

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Resistance to CDK7 inhibitors directed by acquired mutation of a conserved residue in cancer cellsThe EMBO JournalSeptember 8, 2025Chun-Fui Lai, Victoria I Cushing, Ellen Olden, Charlotte L Bevan, R Charles Coombes, Basil J Greber, Laki Buluwela, Simak Ali,Thermo Fisher Scientific,Cat#11496015 Recombinant DNA,Recombinant DNA,Recombinant DNA pNLF1-N NanoLuc Protein Fusion Vector,Promega,Cat#N1351 Rc/CMV cyclin D1 HA,Addgene,Cat#8948 438Sn-CDK7 D97N,This study, 438C-MAT1Δ219-cyclin H,Greber et

(2) LncRNA CTD-2555A7.2 promotes bone formation with LncRNA-specific cascade amplification strategySci RepJuly 1, 2025Fanjin Meng, Kaiyuan Zheng, Meng Deng, Yuwen Ma, Yang Yu, Junxiong Li, Hong Chen, Shan Meng, Bin Guo, Xiaolan Guo, et al.constructed, respectively. For analyze activities of osteogenic transcription factors, PNL1.1 plasmid (N1351, Promega, Fitchburg, WI) were inserted with optimized motif sequence of each transcription factors23.

(3) A Luciferase Immunosorbent Assay Based on Attachment Glycoprotein for the Rapid and Easy Detection of Nipah Virus IgG AntibodiesMicroorganismsMay 14, 2024Xinyue Li, Yuting Fang, Xinyi Huang, Yongkun Zhao, Chengsong Wanprotein assay kit was purchased from Beyotime (Shanghai, China). The pNLF1-N (Promega, Madison, WI, USA, N1351) plasmid was maintained in our laboratory for subsequent vector construction. A Nano-Glo luciferase

(4) A rapid and accurate method for evaluating the degradation of pan-Akt in cells by PROTACs using NanoLuc luciferaseBiol Methods ProtocMarch 5, 2024Xiaojun Ji, Lei Miao, Yebin Wu, Tingli Zhao, Yaxuan Si, Xiaoyun Tan, Qiuhua Zhou, Rui Zuo, Junjie Pei, Jian Wu, et al.in Fig. 1. Materials and methods Materials and reagents The pNLF1-N [CMV/Hygro] Vector (pNLF1-N, N1351), pNLF1-C [CMV/Hygro] Vector (pNLF1-C, N1361), FuGENE HD Transfection Reagent (E2312), and Nano-Glo Luciferase Assay

(5) Lysine deserts and cullin-RING ligase receptors: Navigating untrodden paths in proteostasisiScienceNovember 17, 2023Natalia A. Szulc, Małgorzata Piechota, Lilla Biriczová, Pankaj Thapa, Wojciech Pokrzywatemplates, respectively. NanoLuc fusion vectors were constructed using the parental vectors pNLF1-N (N1351, Promega) and pNLF1-C (N1361, Promega) to obtain N-terminal and C-terminal NanoLuc-tagged protein fusions,

(6) Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stabilityNature structural & molecular biologyNovember 1, 2023Benabdallah NS, Dalal V, Scott RW, Marcous F, Sotiriou A, Kommoss FKF, Pejkovska A, Gaspar L, Wagner L, Sánchez-Rivera FJ, et al.plasmids7. NanoBRET plasmids pHTN-HaloTag-CMV-neo (Promega, G7721) and pNLF1-N-CMV-Hygro (Promega, N1351) were obtained from Promega. Histone H2A cDNAs were amplified by PCR from pCDNA3.1-Flag-H2A and pCDNA3.1-Flag-H2A

(7) REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein BiosensorsBiosensors (Basel)July 28, 2023Katrijn Boon, Nathan Vanalken, Eef Meyen, Dominique Schols, Tom Van Loywere purchased from cDNA Resource Centre and are in the pcDNA3.1(+) backbone. The pNLF1-N vector (#N1351) and pGlosensor-22F cAMP plasmid (#E2301) were purchased from Promega. Pertussis toxin (PTX; #3097)

(8) Cellular Electrical Impedance as a Method to Decipher CCR7 Signalling and Biased AgonismInternational Journal of Molecular SciencesAugust 10, 2022Nathan Vanalken, Katrijn Boon, Jordi Doijen, Dominique Schols, Tom Van Loykindly provided by Dr. A. Inoue (Tohoku University, Sendai, Japan) [34,35,36,37]. pNLF1-N vector (#N1351), pGlosensor-22F cAMP plasmid (#E2301), and pBiT1.1-C and pBiT2.1-N (#N2014) were purchased from Promega.

(9) Luciferase Immunosorbent Assay Based on Multiple E Antigens for the Detection of Chikungunya Virus-Specific IgG AntibodiesMicrobiology SpectrumApril 27, 2022Xiaoxia Li, Xuan Wan, Jinyue Liu, Haiying Wang, Anan Li, Changwen Ke, Shixing Tang, Wei Zhao, Shaoxi Cai, Chengsong Wanfusion protein. CHIKV E1 and E2 DNA sequence fragments were inserted into the pNLF1-N (Promega, USA, N1351) plasmid, and the recombinant plasmid was transfected into 293T cells to express the recombinant protein.

(10) Phase-separated protein droplets of amyotrophic lateral sclerosis-associated p62/SQSTM1 mutants show reduced inner fluidityThe Journal of biological chemistryDecember 1, 2021Faruk MO, Ichimura Y, Kageyama S, Komatsu-Hirota S, El-Gowily AH, Sou YS, Koike M, Noda NN, Komatsu M.and LC3B and FIP200 into the EcoRI and XbaI and EcoRI and NotI sites of pNLF1-N (CMV/Hygro) vector (N1351, Promega), respectively. HEK293T p62-knockout cells were cotransfected with Halo-tagged p62 and NanoLuc-tagged

(11) MACF1 alleviates aging-related osteoporosis via HES1Journal of cellular and molecular medicineJuly 1, 2021Yin C, Tian Y, Hu L, Yu Y, Wu Z, Zhang Y, Wang X, Miao Z, Qian A.of HES1 motif sequence to the promoter region of NanoLuc luciferase gene sequence in PNL1.1 plasmid (N1351; Promega). MACF1 overexpression or MACF1 knockdown MC3T3‐E1 cells and primary BMSCs were seeded on a

(12) miR-129-5p Inhibits Bone Formation Through TCF4Frontiers in cell and developmental biologyJanuary 1, 2020Yin C, Tian Y, Yu Y, Yang C, Su P, Zhao Y, Wang X, Zhang K, Pei J, Li D, et al.sequence (AGATCAAAGG) to the promoter region of nanoluc luciferase gene sequence in PNL1.1 plasmid (N1351, Promega, Fitchburg, WI). MC3T3-E1 cells were seeded in a 6-well plate at 1 × 105 cells per well, and

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