Lambda DNA from Promega

Product Specs
ItemLambda DNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberD1501
Quantity250microg
Nucleic Acid TypeLambda DNA
SpeciesBacteriophage
Size Range48.5 Kbp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Spontaneously directed loop extrusion in SMC complexes emerges from broken detailed balance and anisotropic DNA searchNucleic Acids ResearchAugust 1, 2025Andrea Bonato, Jae-Won Jang, Do-Gyun Kim, Kyoung-Wook Moon, Davide Michieletto, Je-Kyung Ryuand imaging In order to visualize DNA structures, bound by condensin using AFM, we incubated λ-DNA (D1501, Promega) at a concentration of 3 ng/\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym}

(2) Temporally distinct 3D multi-omic dynamics in the developing human brainNatureNovember 14, 2024Matthew G. Heffel, Jingtian Zhou, Yi Zhang, Dong-Sung Lee, Kangcheng Hou, Oier Pastor-Alonso, Kevin D. Abuhanna, Joseph Galasso, Colin Kern, Chu-Yi Tai, et al.containing proteinase K and about 0.05 pg lambda DNA isolated from dcm+ Escherichia coli (Promega number D1501). Single-nucleus DNA methylome library preparation with snmC-seq3 snmC-seq3 is a modification of snmC-seq243

(3) Tet-mediated DNA methylation dynamics affect chromosome organizationNucleic Acids ResearchApril 24, 2024Hao Tian, Pengfei Luan, Yaping Liu, Guoqiang Libisulfite conversion with EZ DNA Methylation-Gold Kit (Zymo, D5005). Unmethylated lambda DNA (Promega, D1501) was sonicated and ligated with the same adapter for the Methyl-HiC sample and then was spiked in at

(4) Chronic graft-versus-host disease is characterized by high levels and distinctive tissue-of-origin patterns of cell-free DNAiScienceNovember 17, 2023Yifan Pang, Temesgen E. Andargie, Moon Kyoo Jang, Hyesik Kong, Woojin Park, Thomas Hill, Neelam Redekar, Yi-Ping Fu, Desai A. Parth, Noa G. Holtzman, et al.cycles),Illumina,20028313 Quant-iT PicoGreen dsDNA assay kit,Thermo Fisher Scientific,P11496 Lambda DNA,Promega,D1501 iTaq Universal SYBR Green Supermix,Bio-Rad,1725122 Primers for Alu115/Alu247,Customized (Available

(5) Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particlesBMC BiologyDecember 1, 2021Marc D. Hein, Prerna Arora, Pavel Marichal-Gallardo, Michael Winkler, Yvonne Genzel, Stefan Pöhlmann, Klaus Schughart, Sascha Y. Kupke, Udo Reichlassay (# P7581; Life Technologies GmbH; Darmstadt, Germany). The standard curve was made with λ DNA (#D1501; Promega; Madison, USA) in the range of 4–250 ng/mL and had a limit of detection of 1.6 ng/mL. The RSD

(6) Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration systemApplied Microbiology and BiotechnologyOctober 1, 2021Marc D. Hein, Anshika Chawla, Maurizio Cattaneo, Sascha Y. Kupke, Yvonne Genzel, Udo Reichlpreviously (Opitz et al. 2007). The standard calibration curve was made using lambda DNA (Promega, D1501). Calculations The total amount of produced DI244 particles for each cultivation was calculated using

(7) DoMY-Seq: A yeast two-hybridâ€“based technique for precision mapping of proteinâ€“protein interaction motifsJournal of Biological ChemistryJanuary 1, 2021Pau Castel, Ann Holtz-Morris, Yongwon Kwon, Bernhard P. Suter, Frank McCormick

(8) A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate studentsJournal of Biological EducationOctober 19, 2020Grant R. Brown, Claire Dagen, Ben Reilly O’Donnell, Jeff A. Gravesexperience each tube of mytaq will yield ca. 30 aliquots of 40µl. Lambda DNA (pack size 250ug) Item code: D1501 PROMEGA £61.20 Oligo's X 4 CHR-2 and LCO1490 sequences INTEGRATED DNA TECHNOLOGIES INC £11.02 Proteinase

(9) Pluripotent stem cells with low differentiation potential contain incompletely reprogrammed DNA replicationJournal of Cell BiologySeptember 7, 2020Theodore Paniza et al.(69506). One microgram of genomic DNA was spiked with 5 ng/ul of unmethylated cl857 Sam7 Lambda DNA (D1501; Promega) to later compute non-conversion rate. The DNA was fragmented to 150–200 bp using a Covaris

(10) Lineage and Parent-of-Origin Effects in DNA Methylation of Honey Bees (Apis mellifera) Revealed by Reciprocal Crosses and Whole-Genome Bisulfite SequencingGenome Biology and EvolutionAugust 1, 2020Xin Wu, David A Galbraith, Paramita Chatterjee, Hyeonsoo Jeong, Christina M Grozinger, Soojin V Yioverhangs and methylated adapters. We added 10 ng of unmethylated lambda phage DNA (Promega, Cat. No. D1501) to the input DNA of each library as a control for bisulfite conversion rates. Bisulfite treatment of

(11) Distinct epigenomic and transcriptomic modifications associated with Wolbachia-mediated asexualityPLOS PathogensMarch 18, 2020Xin Wu, Amelia R. I. Lindsey, Paramita Chatterjee, John H. Werren, Richard Stouthamer, Soojin V. Yi64˚C. As a control for bisulfite conversion, 10ng of unmethylated lambda phage DNA (Promega, Cat. No. D1501) was added to the 1μg of input DNA. Bisulfite-treated DNA fragments remain single-stranded as they are

(12) Cell type-specific epigenetic links to schizophrenia risk in the brainGenome BiologyDecember 1, 2019Isabel Mendizabal et al.and data processing As a control for bisulfite conversion, 10 ng of unmethylated lambda phage DNA (#D1501, Promega) was added to the 1 μg of input DNA. Libraries were made with an in-house Illumina sequencer-compatible

(13) Joint profiling of DNA methylation and chromatin architecture in single cellsNature MethodsOctober 1, 2019Guoqiang Li, Yaping Liu, Yanxiao Zhang, Naoki Kubo, Miao Yu, Rongxin Fang, Manolis Kellis, Bing Renbisulfite conversion with EZ DNA Methylation-Gold™ Kit (Zymo, D5005). Unmethylated lambda DNA (Promega, D1501) was sonicated and ligated with the same adapter for Methyl-HiC sample and then was spiked in at 0.5%

(14) Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodologyAIMS MicrobiologyJanuary 22, 2019Sugenendran Supramani, 1 Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia, Rahayu Ahmad, Zul Ilham, Mohamad Suffian Mohamad Annuar, Anita Klaus, Wan Abd Al Qadr Imad Wan-Mohtar, 2 Halal Action Laboratory, Kolej Permata Insan, University Sains Islam Malaysia, Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia, 3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia, 4 Institute for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbiatriphosphates (dNTPs, 200 μM each) (Promega no. U1511, Madison, OH, USA), 0.5 U DNA polymerase (Promega no. D1501, Madison, OH, USA), supplied PCR buffer (ThermoFisher no. 14966123, Platinum II Green PCR Buffer) and

(15) Global DNA methylation remodeling during direct reprogramming of fibroblasts to neuronseLifeJanuary 15, 2019Luo C, Lee QY, Wapinski O, Castanon R, Nery JR, Mall M, Kareta MS, Cullen SM, Goodell MA, Chang HY, et al.were prepared as previously described (Urich et al., 2015), except regular lambda DNA (Promega cat. # D1501) isolated from dcm +E.Coli was spiked into samples as the control for non-conversion rate. MethylC-seq

(16) Environmental enrichment increases transcriptional and epigenetic differentiation between mouse dorsal and ventral dentate gyrusNature CommunicationsDecember 1, 2018Tie-Yuan Zhang et al.in-house using the Wisegene kit as described in Yu et al.22. Three spike-in control DNAs, lambda DNA (Cat# D1501, Promega), 5 mC (Cat# S001, Wisegene) and 5hmC (Cat# S002, Wisegene) were added to each sample (2.5

(17) Purification of influenza virus-like particles using sulfated cellulose membrane adsorbersJournal of Chemical Technology & BiotechnologyJuly 1, 2018Sofia B Carvalho, A Raquel Fortuna, Michael W Wolff, Cristina Peixoto, Paula M Alves, Udo Reichl, Manuel JT CarrondoQuant‐iT™ Picogreen® dsDNA reagent (P7581, Molecular Probes, USA). The standard curve was prepared with λ‐DNA (#D1501, Promega GmbH, Germany). The assay was carried out in a black 96‐well microplate, flat transparent (3915,

(18) Optimization of cell culture-derived influenza A virus particles purification using sulfated cellulose membrane adsorbersEngineering in Life SciencesJanuary 1, 2018Ana Raquel Fortuna, Florian Taft, Louis Villain, Michael W. Wolff, Udo ReichlThe samples were dialyzed as described in Section 2.6.2. The calibration standard was prepared with λ-DNA (#D1501, Promega GmbH, Mannheim, Germany) in the same dialysis buffer, in the range of 4 to 1000 ngDNA mL−1

(19) MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencingNature ProtocolsMarch 1, 2015Mark A Urich, Joseph R Nery, Ryan Lister, Robert J Schmitz, Joseph R EckerQiagen plant DNeasy and the Qiagen blood and tissue kitsUnmethylated λ phage DNA (Promega, cat. no. D1501)Agencourt AMPure XP (Beckman Coulter, cat. no. A63880)Ethyl alcohol (200 proof, for molecular biology;

(20) DNA target sequence identification mechanism for dimer-active protein complexesNucleic Acids ResearchFebruary 1, 2013Markita P. Landry, Xueqing Zou, Lei Wang, Wai Mun Huang, Klaus Schulten, Yann R. Chemlawas synthesized by filling in the cos ends of λ-DNA with biotinylated dNTPs. Briefly, 1 µg of λ-DNA (D1501, Promega, Madison, WI, USA) was incubated at room temperature for 10 min with 1 U of DNA Polymerase I Large Klenow Fragment

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Lambda DNA

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Lambda DNA from Promega

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